Restriction map for
pF1KB3356
(Circular) (Six-base) MAPSORT of: pF1KB3356.seq Check: 6466 from: 1 to: 5690
pF1KB3356 5690 bp
With 182 enzymes: SgfI *
December 26, 2011 13:17 ..
AccI GT'mk_AC
Cuts at: 642 2728 642
Size: 2086 3604
Acc65I G'GTAC_C
Cuts at: 2706 2706
Size: 5690
AclI AA'CG_TT
Cuts at: 5530 5530
Size: 5690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1859 2441 3515 3947 4694 4962 1859
Size: 582 1074 432 747 268 2587
Fragments arranged by size:
2587 1074 747 582 432 268
AfeI AGC'GCT
Cuts at: 2590 2590
Size: 5690
AflIII A'CryG_T
Cuts at: 880 3042 4146 5123 880
Size: 2162 1104 977 1447
Fragments arranged by size:
2162 1447 1104 977
AgeI A'CCGG_T
Cuts at: 4078 4078
Size: 5690
AlwNI CAG_nnn'CTG
Cuts at: 2995 4562 2995
Size: 1567 4123
ApaLI G'TGCA_C
Cuts at: 2291 4460 2291
Size: 2169 3521
ApoI r'AATT_y
Cuts at: 1357 1470 2694 5043 5299 1357
Size: 113 1224 2349 256 1748
Fragments arranged by size:
2349 1748 1224 256 113
AseI AT'TA_AT
Cuts at: 20 20
Size: 5690
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
AvaI C'yCGr_G
Cuts at: 2207 2710 2207
Size: 503 5187
AvrII C'CTAG_G
Cuts at: 849 849
Size: 5690
BamHI G'GATC_C
Cuts at: 403 2715 403
Size: 2312 3378
BanI G'GyrC_C
Cuts at: 2706 3120 3368 3403 2706
Size: 414 248 35 4993
Fragments arranged by size:
4993 414 248 35
BanII G_rGCy'C
Cuts at: 2704 3734 2704
Size: 1030 4660
BbeI G_GCGC'C
Cuts at: 3124 3372 3124
Size: 248 5442
BciVI GTATCCnnnnn_n'
Cuts at: 3588 4349 5690 3588
Size: 761 1341 3588
Fragments arranged by size:
3588 1341 761
BclI T'GATC_A
Cuts at: 612 984 3210 612
Size: 372 2226 3092
Fragments arranged by size:
3092 2226 372
BglI GCCn_nnn'nGGC
Cuts at: 1430 1430
Size: 5690
BglII A'GATC_T
Cuts at: 701 3205 5242 701
Size: 2504 2037 1149
Fragments arranged by size:
2504 2037 1149
BlpI GC'TnA_GC
Cuts at: 2802 2802
Size: 5690
Bme1580I G_kGCm'C
Cuts at: 2295 3315 3408 4464 2295
Size: 1020 93 1056 3521
Fragments arranged by size:
3521 1056 1020 93
BmgBI CAC'GTC
Cuts at: 1945 2275 1945
Size: 330 5360
BmrI ACTGGGnnnn_n'
Cuts at: 1272 1558 2902 2983 3318 5462 1272
Size: 286 1344 81 335 2144 1500
Fragments arranged by size:
2144 1500 1344 335 286 81
BmtI G_CTAG'C
Cuts at: 1849 1849
Size: 5690
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4057 4057
Size: 5690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 242 2466 2573 141
Size: 101 2224 107 3258
Fragments arranged by size:
3258 2224 107 101
Bpu10I CC'TnA_GC
Cuts at: 1769 1769
Size: 5690
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 2866 3745 4237 4535 1508
Size: 1358 879 492 298 2663
Fragments arranged by size:
2663 1358 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1974 5394 1974
Size: 3420 2270
BsaAI yAC'GTr
Cuts at: 3043 3043
Size: 5690
BsaBI GATnn'nnATC
Cuts at: 6 3204 5241 6
Size: 3198 2037 455
Fragments arranged by size:
3198 2037 455
BsaHI Gr'CG_yC
Cuts at: 3121 3369 4071 5567 3121
Size: 248 702 1496 3244
Fragments arranged by size:
3244 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2879 3400 4078 4352 4499 4910 2879
Size: 521 678 274 147 411 3659
Fragments arranged by size:
3659 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1973 2003 1973
Size: 30 5660
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1087 1733 1087
Size: 646 5044
BseYI C'CCAG_C
Cuts at: 175 2321 3115 3873 4450 4946 175
Size: 2146 794 758 577 496 919
Fragments arranged by size:
2146 919 794 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3232 3278 4486 104
Size: 3128 46 1208 1308
Fragments arranged by size:
3128 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 127 198 276 2295 2704 3482 3672 4464 127
Size: 71 78 2019 409 778 190 792 1353
Fragments arranged by size:
2019 1353 792 778 409 190 78 71
BsmI GAATG_Cn'
Cuts at: 1191 1191
Size: 5690
Bsp1286I G_dGCh'C
Cuts at: 127 198 276 2295 2704 3315 3408 3482 3672
Size: 71 78 2019 409 611 93 74 190
Cuts at: 3672 3734 4464 127
Size: 62 730 1353
Fragments arranged by size:
2019 1353 730 611 409 190 93 78
74 71 62
BspEI T'CCGG_A
Cuts at: 4910 4910
Size: 5690
BspHI T'CATG_A
Cuts at: 4866 4866
Size: 5690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2740 2954 3256 3637 2740
Size: 214 302 381 4793
Fragments arranged by size:
4793 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3982 4036 5520 5685 50
Size: 3932 54 1484 165 55
Fragments arranged by size:
3932 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 895 1305 1419 3602 895
Size: 410 114 2183 2983
Fragments arranged by size:
2983 2183 410 114
BsrFI r'CCGG_y
Cuts at: 3688 3869 4078 3688
Size: 181 209 5300
Fragments arranged by size:
5300 209 181
BssSI C'ACGA_G
Cuts at: 3961 4319 3961
Size: 358 5332
BstAPI GCAn_nnn'nTGC
Cuts at: 3067 3067
Size: 5690
BstBI TT'CG_AA
Cuts at: 4051 4051
Size: 5690
BstXI CCAn_nnnn'nTGG
Cuts at: 653 1222 653
Size: 569 5121
BstYI r'GATC_y
Cuts at: 1 403 701 2452 2715 3182 3205 3540 3786
Size: 402 298 1751 263 467 23 335 246
Cuts at: 3786 4798 4884 4896 5242 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1751 1012 467 449 402 346 335 298
263 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 643 643
Size: 5690
BtgI C'CryG_G
Cuts at: 107 139 1237 2054 107
Size: 32 1098 817 3743
Fragments arranged by size:
3743 1098 817 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1562 3640 3821 3984 1562
Size: 2078 181 163 3268
Fragments arranged by size:
3268 2078 181 163
BtsI GCAGTG_nn'
Cuts at: 1589 2606 2933 1589
Size: 1017 327 4346
Fragments arranged by size:
4346 1017 327
DraI TTT'AAA
Cuts at: 818 1020 2689 818
Size: 202 1669 3819
Fragments arranged by size:
3819 1669 202
DraIII CAC_nnn'GTG
Cuts at: 280 280
Size: 5690
DrdI GACnn_nn'nnGTC
Cuts at: 3396 4254 3396
Size: 858 4832
EaeI y'GGCC_r
Cuts at: 3275 3449 3840 3867 5212 3275
Size: 174 391 27 1345 3753
Fragments arranged by size:
3753 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3275 3275
Size: 5690
EarI CTCTTCn'nnn_
Cuts at: 507 692 1728 2085 2400 2495 3713 3923 507
Size: 185 1036 357 315 95 1218 210 2274
Fragments arranged by size:
2274 1218 1036 357 315 210 185 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3898 4208 4354 5500 3898
Size: 310 146 1146 4088
Fragments arranged by size:
4088 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2702 2702
Size: 5690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 242 1859 2441 2466 2573 3515 3947 4694
Size: 101 1617 582 25 107 942 432 747
Cuts at: 4694 4962 141
Size: 268 869
Fragments arranged by size:
1617 942 869 747 582 432 268 107
101 25
EcoNI CCTnn'n_nnAGG
Cuts at: 1892 1892
Size: 5690
EcoO109I rG'GnC_Cy
Cuts at: 2829 2829
Size: 5690
EcoRI G'AATT_C
Cuts at: 2694 2694
Size: 5690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3709 3709
Size: 5690
FspI TGC'GCA
Cuts at: 3471 3471
Size: 5690
HaeII r_GCGC'y
Cuts at: 2592 3124 3372 4394 5373 2592
Size: 532 248 1022 979 2909
Fragments arranged by size:
2909 1022 979 532 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 532 564 2488 2520 3532 3562 3564 3594 532
Size: 32 1924 32 1012 30 2 30 2628
Fragments arranged by size:
2628 1924 1012 32 32 30 30 2
HincII GTy'rAC
Cuts at: 239 431 1103 2729 239
Size: 192 672 1626 3200
Fragments arranged by size:
3200 1626 672 192
HindIII A'AGCT_T
Cuts at: 1087 1657 1087
Size: 570 5120
KasI G'GCGC_C
Cuts at: 3120 3368 3120
Size: 248 5442
KpnI G_GTAC'C
Cuts at: 2710 2710
Size: 5690
MfeI C'AATT_G
Cuts at: 957 1332 1759 957
Size: 375 427 4888
Fragments arranged by size:
4888 427 375
MluI A'CGCG_T
Cuts at: 5123 5123
Size: 5690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 751 4361 4545 751
Size: 3610 184 1896
Fragments arranged by size:
3610 1896 184
MscI TGG'CCA
Cuts at: 3451 3451
Size: 5690
MslI CAynn'nnrTG
Cuts at: 536 1169 1644 3679 3806 536
Size: 633 475 2035 127 2420
Fragments arranged by size:
2420 2035 633 475 127
MspA1I CmG'CkG
Cuts at: 141 2801 3115 3475 4488 4733 141
Size: 2660 314 360 1013 245 1098
Fragments arranged by size:
2660 1098 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3871 3871
Size: 5690
NarI GG'CG_CC
Cuts at: 3121 3369 3121
Size: 248 5442
NcoI C'CATG_G
Cuts at: 107 1237 107
Size: 1130 4560
NgoMIV G'CCGG_C
Cuts at: 3869 3869
Size: 5690
NheI G'CTAG_C
Cuts at: 1845 1845
Size: 5690
NspI r_CATG'y
Cuts at: 884 2183 2743 3243 4150 884
Size: 1299 560 500 907 2424
Fragments arranged by size:
2424 1299 907 560 500
PciI A'CATG_T
Cuts at: 880 4146 880
Size: 3266 2424
PflMI CCAn_nnn'nTGG
Cuts at: 883 883
Size: 5690
PfoI T'CCnGG_A
Cuts at: 948 2550 948
Size: 1602 4088
PmeI GTTT'AAAC
Cuts at: 2689 2689
Size: 5690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4829 4861 4829
Size: 32 5658
PstI C_TGCA'G
Cuts at: 2737 3422 2737
Size: 685 5005
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5690
PvuII CAG'CTG
Cuts at: 3115 3475 3115
Size: 360 5330
RsrII CG'GwC_CG
Cuts at: 3885 3885
Size: 5690
SacI G_AGCT'C
Cuts at: 2704 2704
Size: 5690
SacII CC_GC'GG
Cuts at: 142 142
Size: 5690
SalI G'TCGA_C
Cuts at: 2727 2727
Size: 5690
SapI GCTCTTCn'nnn_
Cuts at: 3713 3923 3713
Size: 210 5480
SbfI CC_TGCA'GG
Cuts at: 2737 2737
Size: 5690
ScaI AGT'ACT
Cuts at: 5207 5207
Size: 5690
SexAI A'CCwGG_T
Cuts at: 964 964
Size: 5690
SfcI C'TryA_G
Cuts at: 33 333 2733 3418 4411 4602 33
Size: 300 2400 685 993 191 1121
Fragments arranged by size:
2400 1121 993 685 300 191
SfoI GGC'GCC
Cuts at: 3122 3370 3122
Size: 248 5442
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
SmaI CCC'GGG
Cuts at: 2712 2712
Size: 5690
SmlI C'TyrA_G
Cuts at: 1523 2845 3760 4252 4514 1523
Size: 1322 915 492 262 2699
Fragments arranged by size:
2699 1322 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5690
SphI G_CATG'C
Cuts at: 2743 3243 2743
Size: 500 5190
SspI AAT'ATT
Cuts at: 453 901 453
Size: 448 5242
StyI C'CwwG_G
Cuts at: 107 849 1237 2824 3178 107
Size: 742 388 1587 354 2619
Fragments arranged by size:
2619 1587 742 388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1500 3273 3406 3865 4074 1500
Size: 1773 133 459 209 3116
Fragments arranged by size:
3116 1773 459 209 133
TatI w'GTAC_w
Cuts at: 1648 5205 1648
Size: 3557 2133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 5207 5239 2222
Size: 32 2953 32 2673
Fragments arranged by size:
2953 2673 32 32
Tth111I GACn'n_nGTC
Cuts at: 3487 3487
Size: 5690
XbaI T'CTAG_A
Cuts at: 550 2721 550
Size: 2171 3519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3185 3185
Size: 5690
XmaI C'CCGG_G
Cuts at: 2710 2710
Size: 5690
XmnI GAAnn'nnTTC
Cuts at: 1358 1796 1358
Size: 438 5252
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbsI BbvCI BcgI BfrBI BsgI BsiWI
BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI EcoRV
FseI FspAI HpaI NdeI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI