Restriction map for
pF1KB3348
(Circular) (Six-base) MAPSORT of: pF1KB3348.seq Check: 8476 from: 1 to: 5222
pF1KB3348 5222 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1086 1086
Size: 5222
AatII G_ACGT'C
Cuts at: 1526 1526
Size: 5222
AccI GT'mk_AC
Cuts at: 1388 2260 1388
Size: 872 4350
Acc65I G'GTAC_C
Cuts at: 2238 2238
Size: 5222
AclI AA'CG_TT
Cuts at: 5062 5062
Size: 5222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 377 553 3047 3479 3832 4494 160
Size: 217 176 2494 432 353 662 888
Fragments arranged by size:
2494 888 662 432 353 217 176
AflIII A'CryG_T
Cuts at: 2574 4374 4655 2574
Size: 1800 281 3141
Fragments arranged by size:
3141 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2171 2171
Size: 5222
AlwNI CAG_nnn'CTG
Cuts at: 1275 1383 1645 1662 1689 2040 2124 2208 2527
Size: 108 262 17 27 351 84 84 319
Cuts at: 2527 3965 1275
Size: 1438 2532
Fragments arranged by size:
2532 1438 351 319 262 108 84 84
27 17
ApaI G_GGCC'C
Cuts at: 184 1968 184
Size: 1784 3438
ApaLI G'TGCA_C
Cuts at: 4060 4060
Size: 5222
ApoI r'AATT_y
Cuts at: 1513 2052 2226 4575 4831 1513
Size: 539 174 2349 256 1904
Fragments arranged by size:
2349 1904 539 256 174
AseI AT'TA_AT
Cuts at: 20 610 20
Size: 590 4632
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5222
AvaI C'yCGr_G
Cuts at: 1961 2242 1961
Size: 281 4941
BamHI G'GATC_C
Cuts at: 779 2247 779
Size: 1468 3754
BanI G'GyrC_C
Cuts at: 2238 2652 2900 2935 2238
Size: 414 248 35 4525
Fragments arranged by size:
4525 414 248 35
BanII G_rGCy'C
Cuts at: 184 351 440 469 480 871 1319 1968 2236
Size: 167 89 29 11 391 448 649 268
Cuts at: 2236 3266 184
Size: 1030 2140
Fragments arranged by size:
2140 1030 649 448 391 268 167 89
29 11
BbeI G_GCGC'C
Cuts at: 2656 2904 2656
Size: 248 4974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 725 759 1114 1148 90
Size: 34 601 34 355 34 4164
Fragments arranged by size:
4164 601 355 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 867 3120 4176 5222 867
Size: 2253 1056 1046 867
Fragments arranged by size:
2253 1056 1046 867
BclI T'GATC_A
Cuts at: 2742 2742
Size: 5222
BglI GCCn_nnn'nGGC
Cuts at: 740 740
Size: 5222
BglII A'GATC_T
Cuts at: 2737 4774 2737
Size: 2037 3185
BlpI GC'TnA_GC
Cuts at: 439 865 964 1282 1408 2334 439
Size: 426 99 318 126 926 3327
Fragments arranged by size:
3327 926 426 318 126 99
Bme1580I G_kGCm'C
Cuts at: 184 1968 2016 2847 2940 4064 184
Size: 1784 48 831 93 1124 1342
Fragments arranged by size:
1784 1342 1124 831 93 48
BmrI ACTGGGnnnn_n'
Cuts at: 2434 2515 2850 4994 2434
Size: 81 335 2144 2662
Fragments arranged by size:
2662 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 873 3589 873
Size: 2716 2506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 494 525 2116 2149 247
Size: 247 31 1591 33 3320
Fragments arranged by size:
3320 1591 247 33 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 1549 2398 3277 3991 4289 188
Size: 1361 849 879 714 298 1121
Fragments arranged by size:
1361 1121 879 849 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 120 1208 1970 4926 120
Size: 1088 762 2956 416
Fragments arranged by size:
2956 1088 762 416
BsaAI yAC'GTr
Cuts at: 370 2575 370
Size: 2205 3017
BsaBI GATnn'nnATC
Cuts at: 6 640 2736 4773 6
Size: 634 2096 2037 455
Fragments arranged by size:
2096 2037 634 455
BsaHI Gr'CG_yC
Cuts at: 1523 2653 2901 3603 5099 1523
Size: 1130 248 702 1496 1646
Fragments arranged by size:
1646 1496 1130 702 248
BsaWI w'CCGG_w
Cuts at: 1777 2411 2932 3610 4021 4168 4442 1777
Size: 634 521 678 411 147 274 2557
Fragments arranged by size:
2557 678 634 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 290 320 971 1001 1018 1048 290
Size: 30 651 30 17 30 4464
Fragments arranged by size:
4464 651 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 752 789 1165 550
Size: 202 37 376 4607
Fragments arranged by size:
4607 376 202 37
BseYI C'CCAG_C
Cuts at: 243 282 378 2159 2647 3405 4070 4478 243
Size: 39 96 1781 488 758 665 408 987
Fragments arranged by size:
1781 987 758 665 488 408 96 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1240 1288 1396 1717 2044 985
Size: 255 48 108 321 327 4163
Fragments arranged by size:
4163 327 321 255 108 48
BsiEI CG_ry'CG
Cuts at: 104 2764 2810 4040 104
Size: 2660 46 1230 1286
Fragments arranged by size:
2660 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 375 871 920 1319 1565 1639 2132 2236 3014
Size: 496 49 399 246 74 493 104 778
Cuts at: 3014 3204 4064 375
Size: 190 860 1533
Fragments arranged by size:
1533 860 778 496 493 399 246 190
104 74 49
BsmBI CGTCTCn'nnnn_
Cuts at: 1530 1530
Size: 5222
Bsp1286I G_dGCh'C
Cuts at: 184 351 375 440 469 480 871 920 1319
Size: 167 24 65 29 11 391 49 399
Cuts at: 1319 1565 1639 1968 2016 2132 2236 2847 2940
Size: 246 74 329 48 116 104 611 93
Cuts at: 2940 3014 3204 3266 4064 184
Size: 74 190 62 798 1342
Fragments arranged by size:
1342 798 611 399 391 329 246 190
167 116 104 93 74 74 65 62
49 48 29 24 11
BspHI T'CATG_A
Cuts at: 3654 3654
Size: 5222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 560 1086 1244 1739 2272 2486 2788 3169 560
Size: 526 158 495 533 214 302 381 2613
Fragments arranged by size:
2613 533 526 495 381 302 214 158
BsrBI CCG'CTC
Cuts at: 50 312 3514 3568 5052 5217 50
Size: 262 3202 54 1484 165 55
Fragments arranged by size:
3202 1484 262 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1419 1796 1955 3134 1419
Size: 377 159 1179 3507
Fragments arranged by size:
3507 1179 377 159
BsrFI r'CCGG_y
Cuts at: 3220 3401 3220
Size: 181 5041
BsrGI T'GTAC_A
Cuts at: 1034 1034
Size: 5222
BssHII G'CGCG_C
Cuts at: 2109 2109
Size: 5222
BssSI C'ACGA_G
Cuts at: 3493 4201 3493
Size: 708 4514
BstAPI GCAn_nnn'nTGC
Cuts at: 1101 1302 2040 2599 1101
Size: 201 738 559 3724
Fragments arranged by size:
3724 738 559 201
BstBI TT'CG_AA
Cuts at: 3583 3583
Size: 5222
BstXI CCAn_nnnn'nTGG
Cuts at: 221 221
Size: 5222
BstYI r'GATC_y
Cuts at: 1 779 1067 2120 2247 2714 2737 3072 3318
Size: 778 288 1053 127 467 23 335 246
Cuts at: 3318 3624 3636 3722 4774 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1053 1052 778 467 449 335 306 288
246 127 86 23 12
BstZ17I GTA'TAC
Cuts at: 1389 1389
Size: 5222
BtgI C'CryG_G
Cuts at: 1981 2058 1981
Size: 77 5145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3172 3353 3516 3172
Size: 181 163 4878
Fragments arranged by size:
4878 181 163
BtsI GCAGTG_nn'
Cuts at: 298 1085 1722 2032 2465 298
Size: 787 637 310 433 3055
Fragments arranged by size:
3055 787 637 433 310
DraI TTT'AAA
Cuts at: 905 2221 905
Size: 1316 3906
DrdI GACnn_nn'nnGTC
Cuts at: 1984 2928 4272 1984
Size: 944 1344 2934
Fragments arranged by size:
2934 1344 944
EaeI y'GGCC_r
Cuts at: 320 1082 1952 2807 2981 3372 3399 4744 320
Size: 762 870 855 174 391 27 1345 798
Fragments arranged by size:
1345 870 855 798 762 391 174 27
EagI C'GGCC_G
Cuts at: 2807 2807
Size: 5222
EarI CTCTTCn'nnn_
Cuts at: 706 1627 3245 3455 706
Size: 921 1618 210 2473
Fragments arranged by size:
2473 1618 921 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1224 3430 4172 4318 5032 1224
Size: 2206 742 146 714 1414
Fragments arranged by size:
2206 1414 742 714 146
EcoICRI GAG'CTC
Cuts at: 869 1317 2234 869
Size: 448 917 3857
Fragments arranged by size:
3857 917 448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 247 377 494 525 553 2116 2149 3047
Size: 87 130 117 31 28 1563 33 898
Cuts at: 3047 3479 3832 4494 160
Size: 432 353 662 888
Fragments arranged by size:
1563 898 888 662 432 353 130 117
87 33 31 28
EcoNI CCTnn'n_nnAGG
Cuts at: 1999 1999
Size: 5222
EcoO109I rG'GnC_Cy
Cuts at: 180 181 233 362 816 1179 1615 1965 1986
Size: 1 52 129 454 363 436 350 21
Cuts at: 1986 2037 2178 2361 180
Size: 51 141 183 3041
Fragments arranged by size:
3041 454 436 363 350 183 141 129
52 51 21 1
EcoRI G'AATT_C
Cuts at: 2052 2226 2052
Size: 174 5048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1198 3241 1198
Size: 2043 3179
FspI TGC'GCA
Cuts at: 3003 3003
Size: 5222
HaeII r_GCGC'y
Cuts at: 1609 2656 2904 4134 4905 1609
Size: 1047 248 1230 771 1926
Fragments arranged by size:
1926 1230 1047 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 338 370 971 1003 2165 2197 3064 3094 3096
Size: 32 601 32 1162 32 867 30 2
Cuts at: 3096 3126 338
Size: 30 2434
Fragments arranged by size:
2434 1162 867 601 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2261 2261
Size: 5222
HindIII A'AGCT_T
Cuts at: 2152 2152
Size: 5222
KasI G'GCGC_C
Cuts at: 2652 2900 2652
Size: 248 4974
KpnI G_GTAC'C
Cuts at: 2242 2242
Size: 5222
MluI A'CGCG_T
Cuts at: 4655 4655
Size: 5222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1095 3981 4165 1095
Size: 2886 184 2152
Fragments arranged by size:
2886 2152 184
MscI TGG'CCA
Cuts at: 322 1084 1954 2983 322
Size: 762 870 1029 2561
Fragments arranged by size:
2561 1029 870 762
MslI CAynn'nnrTG
Cuts at: 883 1862 3211 3338 883
Size: 979 1349 127 2767
Fragments arranged by size:
2767 1349 979 127
MspA1I CmG'CkG
Cuts at: 443 1024 1200 1926 1983 2205 2333 2647 3007
Size: 581 176 726 57 222 128 314 360
Cuts at: 3007 3791 4036 443
Size: 784 245 1629
Fragments arranged by size:
1629 784 726 581 360 314 245 222
176 128 57
NaeI GCC'GGC
Cuts at: 3403 3403
Size: 5222
NarI GG'CG_CC
Cuts at: 2653 2901 2653
Size: 248 4974
NgoMIV G'CCGG_C
Cuts at: 3401 3401
Size: 5222
NspI r_CATG'y
Cuts at: 1008 2275 2775 4378 1008
Size: 1267 500 1603 1852
Fragments arranged by size:
1852 1603 1267 500
PasI CC'CwG_GG
Cuts at: 1323 1323
Size: 5222
PciI A'CATG_T
Cuts at: 4374 4374
Size: 5222
PflMI CCAn_nnn'nTGG
Cuts at: 1881 2124 1881
Size: 243 4979
PfoI T'CCnGG_A
Cuts at: 471 782 830 1070 1874 1994 2132 2165 471
Size: 311 48 240 804 120 138 33 3528
Fragments arranged by size:
3528 804 311 240 138 120 48 33
PmeI GTTT'AAAC
Cuts at: 2221 2221
Size: 5222
PmlI CAC'GTG
Cuts at: 370 370
Size: 5222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 5190
PpuMI rG'GwC_Cy
Cuts at: 233 816 1615 1986 2178 233
Size: 583 799 371 192 3277
Fragments arranged by size:
3277 799 583 371 192
PspOMI G'GGCC_C
Cuts at: 180 1964 180
Size: 1784 3438
PstI C_TGCA'G
Cuts at: 729 1127 1298 1428 1502 1658 1814 2045 2099
Size: 398 171 130 74 156 156 231 54
Cuts at: 2099 2150 2269 2954 729
Size: 51 119 685 2997
Fragments arranged by size:
2997 685 398 231 171 156 156 130
119 74 54 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5222
PvuII CAG'CTG
Cuts at: 443 1200 1926 2647 3007 443
Size: 757 726 721 360 2658
Fragments arranged by size:
2658 757 726 721 360
RsrII CG'GwC_CG
Cuts at: 3417 3417
Size: 5222
SacI G_AGCT'C
Cuts at: 871 1319 2236 871
Size: 448 917 3857
Fragments arranged by size:
3857 917 448
SacII CC_GC'GG
Cuts at: 1984 1984
Size: 5222
SalI G'TCGA_C
Cuts at: 2259 2259
Size: 5222
SanDI GG'GwC_CC
Cuts at: 233 1615 233
Size: 1382 3840
SapI GCTCTTCn'nnn_
Cuts at: 1627 3245 3455 1627
Size: 1618 210 3394
Fragments arranged by size:
3394 1618 210
SbfI CC_TGCA'GG
Cuts at: 2045 2269 2045
Size: 224 4998
ScaI AGT'ACT
Cuts at: 4739 4739
Size: 5222
SexAI A'CCwGG_T
Cuts at: 497 497
Size: 5222
SfcI C'TryA_G
Cuts at: 33 725 1123 1294 1424 1498 1654 1810 2041
Size: 692 398 171 130 74 156 156 231
Cuts at: 2041 2095 2146 2265 2950 3918 4109 33
Size: 54 51 119 685 968 191 1146
Fragments arranged by size:
1146 968 692 685 398 231 191 171
156 156 130 119 74 54 51
SfoI GGC'GCC
Cuts at: 2654 2902 2654
Size: 248 4974
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5222
SmaI CCC'GGG
Cuts at: 1963 2244 1963
Size: 281 4941
SmlI C'TyrA_G
Cuts at: 203 1564 2377 3292 4006 4268 203
Size: 1361 813 915 714 262 1157
Fragments arranged by size:
1361 1157 915 813 714 262
SpeI A'CTAG_T
Cuts at: 67 2008 67
Size: 1941 3281
SphI G_CATG'C
Cuts at: 2275 2775 2275
Size: 500 4722
SrfI GCCC'GGGC
Cuts at: 1963 1963
Size: 5222
StuI AGG'CCT
Cuts at: 1557 1557
Size: 5222
StyI C'CwwG_G
Cuts at: 400 2356 2710 400
Size: 1956 354 2912
Fragments arranged by size:
2912 1956 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 589 1336 1466 2805 2938 3397 3606 589
Size: 747 130 1339 133 459 209 2205
Fragments arranged by size:
2205 1339 747 459 209 133 130
TatI w'GTAC_w
Cuts at: 1034 4737 1034
Size: 3703 1519
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 290 322 2043 2075 4739 4771 290
Size: 32 1721 32 2664 32 741
Fragments arranged by size:
2664 1721 741 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3019 3019
Size: 5222
XbaI T'CTAG_A
Cuts at: 2253 2253
Size: 5222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2717 2717
Size: 5222
XmaI C'CCGG_G
Cuts at: 1961 2242 1961
Size: 281 4941
XmnI GAAnn'nnTTC
Cuts at: 1058 1435 1058
Size: 377 4845
ZraI GAC'GTC
Cuts at: 1524 1524
Size: 5222
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmI BspEI BstEII Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspXI PsrI SfiI
SgrAI SnaBI SspI SwaI XhoI