Restriction map for
pF1KB3319
(Circular) (Six-base) MAPSORT of: pF1KB3319.seq Check: 8773 from: 1 to: 5531
pF1KB3319 5531 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 296 1545 2013 296
Size: 1249 468 3814
Fragments arranged by size:
3814 1249 468
AccI GT'mk_AC
Cuts at: 1988 2569 1988
Size: 581 4950
Acc65I G'GTAC_C
Cuts at: 2547 2547
Size: 5531
AclI AA'CG_TT
Cuts at: 5371 5371
Size: 5531
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1228 1852 3356 3788 4535 4803 511
Size: 717 624 1504 432 747 268 1239
Fragments arranged by size:
1504 1239 747 717 624 432 268
AfeI AGC'GCT
Cuts at: 1459 1885 2209 1459
Size: 426 324 4781
Fragments arranged by size:
4781 426 324
AflIII A'CryG_T
Cuts at: 170 582 1719 2883 3987 4964 170
Size: 412 1137 1164 1104 977 737
Fragments arranged by size:
1164 1137 1104 977 737 412
AgeI A'CCGG_T
Cuts at: 3919 3919
Size: 5531
AleI CACnn'nnGTG
Cuts at: 2162 2162
Size: 5531
AlwNI CAG_nnn'CTG
Cuts at: 238 486 1656 2428 2836 4403 238
Size: 248 1170 772 408 1567 1366
Fragments arranged by size:
1567 1366 1170 772 408 248
ApaI G_GGCC'C
Cuts at: 259 1370 1791 2515 259
Size: 1111 421 724 3275
Fragments arranged by size:
3275 1111 724 421
ApaLI G'TGCA_C
Cuts at: 283 4301 283
Size: 4018 1513
ApoI r'AATT_y
Cuts at: 2535 4884 5140 2535
Size: 2349 256 2926
Fragments arranged by size:
2926 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5531
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5531
AvaI C'yCGr_G
Cuts at: 401 2551 401
Size: 2150 3381
BamHI G'GATC_C
Cuts at: 1257 2274 2556 1257
Size: 1017 282 4232
Fragments arranged by size:
4232 1017 282
BanI G'GyrC_C
Cuts at: 679 850 1996 2164 2547 2961 3209 3244 679
Size: 171 1146 168 383 414 248 35 2966
Fragments arranged by size:
2966 1146 414 383 248 171 168 35
BanII G_rGCy'C
Cuts at: 259 707 1370 1393 1791 2093 2515 2545 3575
Size: 448 663 23 398 302 422 30 1030
Cuts at: 3575 259
Size: 2215
Fragments arranged by size:
2215 1030 663 448 422 398 302 30
23
BbeI G_GCGC'C
Cuts at: 2000 2965 3213 2000
Size: 965 248 4318
Fragments arranged by size:
4318 965 248
BbsI GAAGACnn'nnnn_
Cuts at: 634 634
Size: 5531
BbvCI CC'TCA_GC
Cuts at: 2285 2285
Size: 5531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1136 1170 1136
Size: 34 5497
BciVI GTATCCnnnnn_n'
Cuts at: 2358 3429 4190 5531 2358
Size: 1071 761 1341 2358
Fragments arranged by size:
2358 1341 1071 761
BclI T'GATC_A
Cuts at: 3051 3051
Size: 5531
BglI GCCn_nnn'nGGC
Cuts at: 1858 2519 1858
Size: 661 4870
BglII A'GATC_T
Cuts at: 365 464 3046 5083 365
Size: 99 2582 2037 813
Fragments arranged by size:
2582 2037 813 99
BlpI GC'TnA_GC
Cuts at: 373 1897 2204 2211 2643 373
Size: 1524 307 7 432 3261
Fragments arranged by size:
3261 1524 432 307 7
Bme1580I G_kGCm'C
Cuts at: 259 287 347 684 855 1070 1370 1791 2469
Size: 28 60 337 171 215 300 421 678
Cuts at: 2469 2515 3156 3249 4305 259
Size: 46 641 93 1056 1485
Fragments arranged by size:
1485 1056 678 641 421 337 300 215
171 93 60 46 28
BmgBI CAC'GTC
Cuts at: 171 171
Size: 5531
BmrI ACTGGGnnnn_n'
Cuts at: 654 723 894 2743 2824 3159 5303 654
Size: 69 171 1849 81 335 2144 882
Fragments arranged by size:
2144 1849 882 335 171 81 69
BmtI G_CTAG'C
Cuts at: 971 971
Size: 5531
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1929 3898 1929
Size: 1969 3562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 508 679 757 2053 2182 2280 2368 2395
Size: 244 171 78 1296 129 98 88 27
Cuts at: 2395 2461 264
Size: 66 3334
Fragments arranged by size:
3334 1296 244 171 129 98 88 78
66 27
Bpu10I CC'TnA_GC
Cuts at: 2285 2332 2285
Size: 47 5484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 447 2707 3586 4078 4376 447
Size: 2260 879 492 298 1602
Fragments arranged by size:
2260 1602 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 604 1327 1900 5235 604
Size: 723 573 3335 900
Fragments arranged by size:
3335 900 723 573
BsaAI yAC'GTr
Cuts at: 321 583 1720 2016 2884 321
Size: 262 1137 296 868 2968
Fragments arranged by size:
2968 1137 868 296 262
BsaBI GATnn'nnATC
Cuts at: 6 3045 5082 6
Size: 3039 2037 455
Fragments arranged by size:
3039 2037 455
BsaHI Gr'CG_yC
Cuts at: 1379 1997 2962 3210 3912 5408 1379
Size: 618 965 248 702 1496 1502
Fragments arranged by size:
1502 1496 965 702 618 248
BsaWI w'CCGG_w
Cuts at: 2720 3241 3919 4193 4340 4751 2720
Size: 521 678 274 147 411 3500
Fragments arranged by size:
3500 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1228 1258 1228
Size: 30 5501
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 261 286 841 970 1249 1333 1468 1858 2488
Size: 25 555 129 279 84 135 390 630
Cuts at: 2488 261
Size: 3304
Fragments arranged by size:
3304 630 555 390 279 135 129 84
25
BseYI C'CCAG_C
Cuts at: 1063 1656 1779 2956 3714 4291 4787 1063
Size: 593 123 1177 758 577 496 1807
Fragments arranged by size:
1807 1177 758 593 577 496 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 499 961 1180 1423 1648 1675 2038 475
Size: 24 462 219 243 225 27 363 3968
Fragments arranged by size:
3968 462 363 243 225 219 27 24
BsiEI CG_ry'CG
Cuts at: 104 3073 3119 4327 104
Size: 2969 46 1208 1308
Fragments arranged by size:
2969 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 287 707 1478 2093 2450 2545 3323 3513 4305
Size: 420 771 615 357 95 778 190 792
Cuts at: 4305 287
Size: 1513
Fragments arranged by size:
1513 792 778 771 615 420 357 190
95
Bsp1286I G_dGCh'C
Cuts at: 259 287 347 684 707 855 1070 1370 1393
Size: 28 60 337 23 148 215 300 23
Cuts at: 1393 1478 1791 2093 2450 2469 2515 2545 3156
Size: 85 313 302 357 19 46 30 611
Cuts at: 3156 3249 3323 3513 3575 4305 259
Size: 93 74 190 62 730 1485
Fragments arranged by size:
1485 730 611 357 337 313 302 300
215 190 148 93 85 74 62 60
46 30 28 23 23 19
BspEI T'CCGG_A
Cuts at: 4751 4751
Size: 5531
BspHI T'CATG_A
Cuts at: 4707 4707
Size: 5531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 296 1202 1545 2013 2197 2581 2795 3097 3478
Size: 906 343 468 184 384 214 302 381
Cuts at: 3478 296
Size: 2349
Fragments arranged by size:
2349 906 468 384 381 343 302 214
184
BsrBI CCG'CTC
Cuts at: 50 1601 3823 3877 5361 5526 50
Size: 1551 2222 54 1484 165 55
Fragments arranged by size:
2222 1551 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3443 3443
Size: 5531
BsrFI r'CCGG_y
Cuts at: 1442 1739 1852 2218 3529 3710 3919 1442
Size: 297 113 366 1311 181 209 3054
Fragments arranged by size:
3054 1311 366 297 209 181 113
BssHII G'CGCG_C
Cuts at: 152 2177 2179 152
Size: 2025 2 3504
Fragments arranged by size:
3504 2025 2
BssSI C'ACGA_G
Cuts at: 1177 1198 2470 3802 4160 1177
Size: 21 1272 1332 358 2548
Fragments arranged by size:
2548 1332 1272 358 21
BstAPI GCAn_nnn'nTGC
Cuts at: 2428 2908 2428
Size: 480 5051
BstBI TT'CG_AA
Cuts at: 3892 3892
Size: 5531
BstXI CCAn_nnnn'nTGG
Cuts at: 540 1032 1158 540
Size: 492 126 4913
Fragments arranged by size:
4913 492 126
BstYI r'GATC_y
Cuts at: 1 365 464 1257 1292 2274 2556 3023 3046
Size: 364 99 793 35 982 282 467 23
Cuts at: 3046 3381 3627 4639 4725 4737 5083 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 982 793 467 449 364 346 335
282 246 99 86 35 23 12
Bsu36I CC'TnA_GG
Cuts at: 2399 2399
Size: 5531
BtgI C'CryG_G
Cuts at: 107 1109 1394 1976 107
Size: 1002 285 582 3662
Fragments arranged by size:
3662 1002 582 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3481 3662 3825 3481
Size: 181 163 5187
Fragments arranged by size:
5187 181 163
BtsI GCAGTG_nn'
Cuts at: 691 962 1603 1714 2774 691
Size: 271 641 111 1060 3448
Fragments arranged by size:
3448 1060 641 271 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1851 1886 1851
Size: 35 5496
DraI TTT'AAA
Cuts at: 2530 2530
Size: 5531
DrdI GACnn_nn'nnGTC
Cuts at: 3237 4095 3237
Size: 858 4673
EaeI y'GGCC_r
Cuts at: 110 224 1050 1499 1805 1859 2147 3116 3290
Size: 114 826 449 306 54 288 969 174
Cuts at: 3290 3681 3708 5053 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 969 826 588 449 391 306 288
174 114 54 27
EagI C'GGCC_G
Cuts at: 3116 3116
Size: 5531
EarI CTCTTCn'nnn_
Cuts at: 983 1132 1522 3554 3764 983
Size: 149 390 2032 210 2750
Fragments arranged by size:
2750 2032 390 210 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 790 3739 4049 4195 5341 790
Size: 2949 310 146 1146 980
Fragments arranged by size:
2949 1146 980 310 146
EcoICRI GAG'CTC
Cuts at: 705 2091 2543 705
Size: 1386 452 3693
Fragments arranged by size:
3693 1386 452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 508 511 679 757 1228 1852 2053 2182
Size: 244 3 168 78 471 624 201 129
Cuts at: 2182 2280 2368 2395 2461 3356 3788 4535 4803
Size: 98 88 27 66 895 432 747 268
Cuts at: 4803 264
Size: 992
Fragments arranged by size:
992 895 747 624 471 432 268 244
201 168 129 98 88 78 66 27
3
EcoNI CCTnn'n_nnAGG
Cuts at: 877 1198 2440 877
Size: 321 1242 3968
Fragments arranged by size:
3968 1242 321
EcoO109I rG'GnC_Cy
Cuts at: 256 537 1017 1203 1366 1787 1788 2390 2403
Size: 281 480 186 163 421 1 602 13
Cuts at: 2403 2512 2670 256
Size: 109 158 3117
Fragments arranged by size:
3117 602 480 421 281 186 163 158
109 13 1
EcoRI G'AATT_C
Cuts at: 2535 2535
Size: 5531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 651 753 3550 651
Size: 102 2797 2632
Fragments arranged by size:
2797 2632 102
FspI TGC'GCA
Cuts at: 2116 3312 2116
Size: 1196 4335
HaeII r_GCGC'y
Cuts at: 1404 1461 1887 2000 2053 2129 2211 2965 3213
Size: 57 426 113 53 76 82 754 248
Cuts at: 3213 4235 5214 1404
Size: 1022 979 1721
Fragments arranged by size:
1721 1022 979 754 426 248 113 82
76 57 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2286 2318 3373 3403 3405 3435 2286
Size: 32 1055 30 2 30 4382
Fragments arranged by size:
4382 1055 32 30 30 2
HincII GTy'rAC
Cuts at: 2570 2570
Size: 5531
KasI G'GCGC_C
Cuts at: 1996 2961 3209 1996
Size: 965 248 4318
Fragments arranged by size:
4318 965 248
KpnI G_GTAC'C
Cuts at: 2551 2551
Size: 5531
MluI A'CGCG_T
Cuts at: 4964 4964
Size: 5531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2028 4202 4386 2028
Size: 2174 184 3173
Fragments arranged by size:
3173 2174 184
MscI TGG'CCA
Cuts at: 112 226 1501 1861 3292 112
Size: 114 1275 360 1431 2351
Fragments arranged by size:
2351 1431 1275 360 114
MslI CAynn'nnrTG
Cuts at: 647 1957 2162 3520 3647 647
Size: 1310 205 1358 127 2531
Fragments arranged by size:
2531 1358 1310 205 127
MspA1I CmG'CkG
Cuts at: 309 416 506 1437 1656 1901 2242 2310 2642
Size: 107 90 931 219 245 341 68 332
Cuts at: 2642 2956 3316 4329 4574 309
Size: 314 360 1013 245 1266
Fragments arranged by size:
1266 1013 931 360 341 332 314 245
245 219 107 90 68
NaeI GCC'GGC
Cuts at: 1854 2220 3712 1854
Size: 366 1492 3673
Fragments arranged by size:
3673 1492 366
NarI GG'CG_CC
Cuts at: 1997 2962 3210 1997
Size: 965 248 4318
Fragments arranged by size:
4318 965 248
NcoI C'CATG_G
Cuts at: 107 1109 107
Size: 1002 4529
NgoMIV G'CCGG_C
Cuts at: 1852 2218 3710 1852
Size: 366 1492 3673
Fragments arranged by size:
3673 1492 366
NheI G'CTAG_C
Cuts at: 967 967
Size: 5531
NspI r_CATG'y
Cuts at: 1188 2584 3084 3991 1188
Size: 1396 500 907 2728
Fragments arranged by size:
2728 1396 907 500
PasI CC'CwG_GG
Cuts at: 2250 2508 2250
Size: 258 5273
PciI A'CATG_T
Cuts at: 3987 3987
Size: 5531
PflMI CCAn_nnn'nTGG
Cuts at: 309 606 1656 309
Size: 297 1050 4184
Fragments arranged by size:
4184 1050 297
PfoI T'CCnGG_A
Cuts at: 1733 1733
Size: 5531
PmeI GTTT'AAAC
Cuts at: 2530 2530
Size: 5531
PmlI CAC'GTG
Cuts at: 583 1720 2016 583
Size: 1137 296 4098
Fragments arranged by size:
4098 1137 296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 4670
Size: 32 5499
PpuMI rG'GwC_Cy
Cuts at: 537 1017 1203 2403 537
Size: 480 186 1200 3665
Fragments arranged by size:
3665 1200 480 186
PspOMI G'GGCC_C
Cuts at: 255 1366 1787 2511 255
Size: 1111 421 724 3275
Fragments arranged by size:
3275 1111 724 421
PstI C_TGCA'G
Cuts at: 1709 2227 2578 3263 1709
Size: 518 351 685 3977
Fragments arranged by size:
3977 685 518 351
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5531
PvuII CAG'CTG
Cuts at: 309 416 1437 1656 2242 2310 2956 3316 309
Size: 107 1021 219 586 68 646 360 2524
Fragments arranged by size:
2524 1021 646 586 360 219 107 68
RsrII CG'GwC_CG
Cuts at: 3726 3726
Size: 5531
SacI G_AGCT'C
Cuts at: 707 2093 2545 707
Size: 1386 452 3693
Fragments arranged by size:
3693 1386 452
SalI G'TCGA_C
Cuts at: 2568 2568
Size: 5531
SapI GCTCTTCn'nnn_
Cuts at: 983 3554 3764 983
Size: 2571 210 2750
Fragments arranged by size:
2750 2571 210
SbfI CC_TGCA'GG
Cuts at: 2578 2578
Size: 5531
ScaI AGT'ACT
Cuts at: 5048 5048
Size: 5531
SfcI C'TryA_G
Cuts at: 33 1705 2223 2574 3259 4252 4443 33
Size: 1672 518 351 685 993 191 1121
Fragments arranged by size:
1672 1121 993 685 518 351 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2519 2519
Size: 5531
SfoI GGC'GCC
Cuts at: 1998 2963 3211 1998
Size: 965 248 4318
Fragments arranged by size:
4318 965 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5531
SgrAI Cr'CCGG_yG
Cuts at: 1442 1442
Size: 5531
SmaI CCC'GGG
Cuts at: 2553 2553
Size: 5531
SmlI C'TyrA_G
Cuts at: 426 2686 3601 4093 4355 426
Size: 2260 915 492 262 1602
Fragments arranged by size:
2260 1602 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5531
SphI G_CATG'C
Cuts at: 1188 2584 3084 1188
Size: 1396 500 3635
Fragments arranged by size:
3635 1396 500
StyI C'CwwG_G
Cuts at: 107 164 974 998 1109 1619 1662 2517 2665
Size: 57 810 24 111 510 43 855 148
Cuts at: 2665 3019 107
Size: 354 2619
Fragments arranged by size:
2619 855 810 510 354 148 111 57
43 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 838 3114 3247 3706 3915 838
Size: 2276 133 459 209 2454
Fragments arranged by size:
2454 2276 459 209 133
TatI w'GTAC_w
Cuts at: 275 1244 5046 275
Size: 969 3802 760
Fragments arranged by size:
3802 969 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 834 866 1526 1558 5048 5080 834
Size: 32 660 32 3490 32 1285
Fragments arranged by size:
3490 1285 660 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 337 886 3328 337
Size: 549 2442 2540
Fragments arranged by size:
2540 2442 549
XbaI T'CTAG_A
Cuts at: 2562 2562
Size: 5531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 791 3026 791
Size: 2235 3296
XmaI C'CCGG_G
Cuts at: 2551 2551
Size: 5531
XmnI GAAnn'nnTTC
Cuts at: 736 736
Size: 5531
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BfrBI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI