Restriction map for
pF1KB3316
(Circular) (Six-base) MAPSORT of: pF1KB3316.seq Check: 5160 from: 1 to: 5330
pF1KB3316 5330 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1422 2090 2368 1422
Size: 668 278 4384
Fragments arranged by size:
4384 668 278
Acc65I G'GTAC_C
Cuts at: 2346 2346
Size: 5330
AclI AA'CG_TT
Cuts at: 5170 5170
Size: 5330
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 545 1007 1181 3155 3587 3940 4602 254
Size: 291 462 174 1974 432 353 662 982
Fragments arranged by size:
1974 982 662 462 432 353 291 174
AfeI AGC'GCT
Cuts at: 171 171
Size: 5330
AflIII A'CryG_T
Cuts at: 2682 4482 4763 2682
Size: 1800 281 3249
Fragments arranged by size:
3249 1800 281
AgeI A'CCGG_T
Cuts at: 1942 1942
Size: 5330
AhdI GACnn_n'nnGTC
Cuts at: 861 2070 861
Size: 1209 4121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 413 413
Size: 5330
AlwNI CAG_nnn'CTG
Cuts at: 379 2106 2635 4073 379
Size: 1727 529 1438 1636
Fragments arranged by size:
1727 1636 1438 529
ApaLI G'TGCA_C
Cuts at: 4168 4168
Size: 5330
ApoI r'AATT_y
Cuts at: 619 1124 1499 1616 1630 1778 1851 2014 2040
Size: 505 375 117 14 148 73 163 26
Cuts at: 2040 2334 4683 4939 619
Size: 294 2349 256 1010
Fragments arranged by size:
2349 1010 505 375 294 256 163 148
117 73 26 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 5330
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
AvaI C'yCGr_G
Cuts at: 2350 2350
Size: 5330
BamHI G'GATC_C
Cuts at: 2355 2355
Size: 5330
BanI G'GyrC_C
Cuts at: 2206 2346 2760 3008 3043 2206
Size: 140 414 248 35 4493
Fragments arranged by size:
4493 414 248 140 35
BanII G_rGCy'C
Cuts at: 160 2344 3374 160
Size: 2184 1030 2116
Fragments arranged by size:
2184 2116 1030
BbeI G_GCGC'C
Cuts at: 2764 3012 2764
Size: 248 5082
BbsI GAAGACnn'nnnn_
Cuts at: 1816 1816
Size: 5330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5296
BciVI GTATCCnnnnn_n'
Cuts at: 3228 4284 5330 3228
Size: 1056 1046 3228
Fragments arranged by size:
3228 1056 1046
BclI T'GATC_A
Cuts at: 2850 2850
Size: 5330
BglII A'GATC_T
Cuts at: 2845 4882 2845
Size: 2037 3293
BlpI GC'TnA_GC
Cuts at: 2285 2442 2285
Size: 157 5173
Bme1580I G_kGCm'C
Cuts at: 2955 3048 4172 2955
Size: 93 1124 4113
Fragments arranged by size:
4113 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 300 2157 2542 2623 2958 5102 300
Size: 1857 385 81 335 2144 528
Fragments arranged by size:
2144 1857 528 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3697 3697
Size: 5330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 800
Size: 5330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2506 3385 4099 4397 2506
Size: 879 714 298 3439
Fragments arranged by size:
3439 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 431 1472 5034 431
Size: 1041 3562 727
Fragments arranged by size:
3562 1041 727
BsaAI yAC'GTr
Cuts at: 2683 2683
Size: 5330
BsaBI GATnn'nnATC
Cuts at: 6 2844 4881 6
Size: 2838 2037 455
Fragments arranged by size:
2838 2037 455
BsaHI Gr'CG_yC
Cuts at: 2761 3009 3711 5207 2761
Size: 248 702 1496 2884
Fragments arranged by size:
2884 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1764 1942 2519 3040 3718 4129 4276 4550 1764
Size: 178 577 521 678 411 147 274 2544
Fragments arranged by size:
2544 678 577 521 411 274 178 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 1902 1932 145
Size: 30 1727 30 3543
Fragments arranged by size:
3543 1727 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 229 578 226
Size: 3 349 4978
Fragments arranged by size:
4978 349 3
BseYI C'CCAG_C
Cuts at: 2755 3513 4178 4586 2755
Size: 758 665 408 3499
Fragments arranged by size:
3499 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1064 1064
Size: 5330
BsiEI CG_ry'CG
Cuts at: 104 2872 2918 4148 104
Size: 2768 46 1230 1286
Fragments arranged by size:
2768 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2126 2344 3122 3312 4172 2126
Size: 218 778 190 860 3284
Fragments arranged by size:
3284 860 778 218 190
BsmI GAATG_Cn'
Cuts at: 971 1013 1220 971
Size: 42 207 5081
Fragments arranged by size:
5081 207 42
Bsp1286I G_dGCh'C
Cuts at: 160 2126 2344 2955 3048 3122 3312 3374 4172
Size: 1966 218 611 93 74 190 62 798
Cuts at: 4172 160
Size: 1318
Fragments arranged by size:
1966 1318 798 611 218 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1764 1764
Size: 5330
BspHI T'CATG_A
Cuts at: 1829 3762 1829
Size: 1933 3397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1152 2380 2594 2896 3277 1152
Size: 1228 214 302 381 3205
Fragments arranged by size:
3205 1228 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1813 2273 3622 3676 5160 5325 50
Size: 1763 460 1349 54 1484 165 55
Fragments arranged by size:
1763 1484 1349 460 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3242 3242
Size: 5330
BsrFI r'CCGG_y
Cuts at: 1942 3328 3509 1942
Size: 1386 181 3763
Fragments arranged by size:
3763 1386 181
BsrGI T'GTAC_A
Cuts at: 1317 1317
Size: 5330
BssSI C'ACGA_G
Cuts at: 3601 4309 3601
Size: 708 4622
BstAPI GCAn_nnn'nTGC
Cuts at: 2707 2707
Size: 5330
BstBI TT'CG_AA
Cuts at: 1776 3691 1776
Size: 1915 3415
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5330
BstYI r'GATC_y
Cuts at: 1 297 2355 2822 2845 3180 3426 3732 3744
Size: 296 2058 467 23 335 246 306 12
Cuts at: 3744 3830 4882 1
Size: 86 1052 449
Fragments arranged by size:
2058 1052 467 449 335 306 296 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1423 1423
Size: 5330
BtgI C'CryG_G
Cuts at: 133 2210 133
Size: 2077 3253
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3280 3461 3624 3280
Size: 181 163 4986
Fragments arranged by size:
4986 181 163
BtsI GCAGTG_nn'
Cuts at: 1457 1844 2573 1457
Size: 387 729 4214
Fragments arranged by size:
4214 729 387
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 959 994 959
Size: 35 5295
DraI TTT'AAA
Cuts at: 320 1140 1874 2329 320
Size: 820 734 455 3321
Fragments arranged by size:
3321 820 734 455
DraIII CAC_nnn'GTG
Cuts at: 1231 2026 1231
Size: 795 4535
DrdI GACnn_nn'nnGTC
Cuts at: 3036 4380 3036
Size: 1344 3986
EaeI y'GGCC_r
Cuts at: 2915 3089 3480 3507 4852 2915
Size: 174 391 27 1345 3393
Fragments arranged by size:
3393 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2915 2915
Size: 5330
EarI CTCTTCn'nnn_
Cuts at: 1113 3353 3563 1113
Size: 2240 210 2880
Fragments arranged by size:
2880 2240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 142 1121 3538 4280 4426 5140 142
Size: 979 2417 742 146 714 332
Fragments arranged by size:
2417 979 742 714 332 146
EcoICRI GAG'CTC
Cuts at: 2342 2342
Size: 5330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 545 800 1007 1181 3155 3587 3940 4602
Size: 291 255 207 174 1974 432 353 662
Cuts at: 4602 254
Size: 982
Fragments arranged by size:
1974 982 662 432 353 291 255 207
174
EcoNI CCTnn'n_nnAGG
Cuts at: 911 911
Size: 5330
EcoO109I rG'GnC_Cy
Cuts at: 2064 2469 2064
Size: 405 4925
EcoRI G'AATT_C
Cuts at: 2040 2334 2040
Size: 294 5036
EcoRV GAT'ATC
Cuts at: 468 468
Size: 5330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 372 976 3349 372
Size: 604 2373 2353
Fragments arranged by size:
2373 2353 604
FspI TGC'GCA
Cuts at: 3111 3111
Size: 5330
HaeII r_GCGC'y
Cuts at: 173 2764 3012 4242 5013 173
Size: 2591 248 1230 771 490
Fragments arranged by size:
2591 1230 771 490 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 177 847 879 2056 2088 2100 2132 3172
Size: 32 670 32 1177 32 12 32 1040
Cuts at: 3172 3202 3204 3234 145
Size: 30 2 30 2241
Fragments arranged by size:
2241 1177 1040 670 32 32 32 32
30 30 12 2
HincII GTy'rAC
Cuts at: 734 2369 734
Size: 1635 3695
HpaI GTT'AAC
Cuts at: 734 734
Size: 5330
KasI G'GCGC_C
Cuts at: 2760 3008 2760
Size: 248 5082
KpnI G_GTAC'C
Cuts at: 2350 2350
Size: 5330
MfeI C'AATT_G
Cuts at: 1325 1325
Size: 5330
MluI A'CGCG_T
Cuts at: 4763 4763
Size: 5330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 4089 4273 188
Size: 3901 184 1245
Fragments arranged by size:
3901 1245 184
MscI TGG'CCA
Cuts at: 3091 3091
Size: 5330
MslI CAynn'nnrTG
Cuts at: 3319 3446 3319
Size: 127 5203
MspA1I CmG'CkG
Cuts at: 114 123 1806 2289 2441 2755 3115 3899 4144
Size: 9 1683 483 152 314 360 784 245
Cuts at: 4144 114
Size: 1300
Fragments arranged by size:
1683 1300 784 483 360 314 245 152
9
NaeI GCC'GGC
Cuts at: 3511 3511
Size: 5330
NarI GG'CG_CC
Cuts at: 2761 3009 2761
Size: 248 5082
NcoI C'CATG_G
Cuts at: 2210 2210
Size: 5330
NgoMIV G'CCGG_C
Cuts at: 3509 3509
Size: 5330
NspI r_CATG'y
Cuts at: 2383 2883 4486 2383
Size: 500 1603 3227
Fragments arranged by size:
3227 1603 500
PciI A'CATG_T
Cuts at: 4482 4482
Size: 5330
PmeI GTTT'AAAC
Cuts at: 2329 2329
Size: 5330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 428 460 583 615 3776 3808 428
Size: 32 123 32 3161 32 1950
Fragments arranged by size:
3161 1950 123 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2064 2064
Size: 5330
PstI C_TGCA'G
Cuts at: 2377 3062 2377
Size: 685 4645
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5330
PvuII CAG'CTG
Cuts at: 123 1806 2755 3115 123
Size: 1683 949 360 2338
Fragments arranged by size:
2338 1683 949 360
RsrII CG'GwC_CG
Cuts at: 3525 3525
Size: 5330
SacI G_AGCT'C
Cuts at: 2344 2344
Size: 5330
SalI G'TCGA_C
Cuts at: 2367 2367
Size: 5330
SapI GCTCTTCn'nnn_
Cuts at: 3353 3563 3353
Size: 210 5120
SbfI CC_TGCA'GG
Cuts at: 2377 2377
Size: 5330
ScaI AGT'ACT
Cuts at: 4847 4847
Size: 5330
SexAI A'CCwGG_T
Cuts at: 2066 2066
Size: 5330
SfcI C'TryA_G
Cuts at: 33 2373 3058 4026 4217 33
Size: 2340 685 968 191 1146
Fragments arranged by size:
2340 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2762 3010 2762
Size: 248 5082
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
SmaI CCC'GGG
Cuts at: 2352 2352
Size: 5330
SmlI C'TyrA_G
Cuts at: 2485 3400 4114 4376 2485
Size: 915 714 262 3439
Fragments arranged by size:
3439 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5330
SphI G_CATG'C
Cuts at: 2383 2883 2383
Size: 500 4830
SspI AAT'ATT
Cuts at: 1991 1991
Size: 5330
StuI AGG'CCT
Cuts at: 1158 1158
Size: 5330
StyI C'CwwG_G
Cuts at: 912 2134 2210 2464 2818 912
Size: 1222 76 254 354 3424
Fragments arranged by size:
3424 1222 354 254 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 455 2913 3046 3505 3714 455
Size: 2458 133 459 209 2071
Fragments arranged by size:
2458 2071 459 209 133
TatI w'GTAC_w
Cuts at: 1317 4845 1317
Size: 3528 1802
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 893 925 1553 1585 1902 1934 4847 4879 893
Size: 32 628 32 317 32 2913 32 1344
Fragments arranged by size:
2913 1344 628 317 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3127 3127
Size: 5330
XbaI T'CTAG_A
Cuts at: 2361 2361
Size: 5330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2825 2825
Size: 5330
XmaI C'CCGG_G
Cuts at: 2350 2350
Size: 5330
XmnI GAAnn'nnTTC
Cuts at: 1502 1691 1772 1502
Size: 189 81 5060
Fragments arranged by size:
5060 189 81
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmBI BssHII BstEII Bsu36I ClaI FseI FspAI HindIII
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI