Restriction map for
pF1KB3313
(Circular) (Six-base) MAPSORT of: pF1KB3313.seq Check: 952 from: 1 to: 4772
pF1KB3313 4772 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 406 406
Size: 4772
AccI GT'mk_AC
Cuts at: 1810 1810
Size: 4772
Acc65I G'GTAC_C
Cuts at: 1788 1788
Size: 4772
AclI AA'CG_TT
Cuts at: 4612 4612
Size: 4772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 524 536 545 1082 2597 3029 3776 4044
Size: 217 12 9 537 1515 432 747 268
Cuts at: 4044 307
Size: 1035
Fragments arranged by size:
1515 1035 747 537 432 268 217 12
9
AflIII A'CryG_T
Cuts at: 1184 1764 2124 3228 4205 1184
Size: 580 360 1104 977 1751
Fragments arranged by size:
1751 1104 977 580 360
AgeI A'CCGG_T
Cuts at: 3160 3160
Size: 4772
AhdI GACnn_n'nnGTC
Cuts at: 402 402
Size: 4772
AleI CACnn'nnGTG
Cuts at: 880 880
Size: 4772
AlwNI CAG_nnn'CTG
Cuts at: 988 1284 1352 2077 3644 988
Size: 296 68 725 1567 2116
Fragments arranged by size:
2116 1567 725 296 68
ApaI G_GGCC'C
Cuts at: 1041 1138 1041
Size: 97 4675
ApaLI G'TGCA_C
Cuts at: 3542 3542
Size: 4772
ApoI r'AATT_y
Cuts at: 241 1776 4125 4381 241
Size: 1535 2349 256 632
Fragments arranged by size:
2349 1535 632 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4772
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4772
AvaI C'yCGr_G
Cuts at: 1792 1792
Size: 4772
AvrII C'CTAG_G
Cuts at: 1386 1386
Size: 4772
BamHI G'GATC_C
Cuts at: 1104 1157 1797 1104
Size: 53 640 4079
Fragments arranged by size:
4079 640 53
BanI G'GyrC_C
Cuts at: 1788 2202 2450 2485 1788
Size: 414 248 35 4075
Fragments arranged by size:
4075 414 248 35
BanII G_rGCy'C
Cuts at: 597 1041 1138 1786 2816 597
Size: 444 97 648 1030 2553
Fragments arranged by size:
2553 1030 648 444 97
BbeI G_GCGC'C
Cuts at: 2206 2454 2206
Size: 248 4524
BbsI GAAGACnn'nnnn_
Cuts at: 542 1378 542
Size: 836 3936
BciVI GTATCCnnnnn_n'
Cuts at: 2670 3431 4772 2670
Size: 761 1341 2670
Fragments arranged by size:
2670 1341 761
BclI T'GATC_A
Cuts at: 2292 2292
Size: 4772
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4772
BglII A'GATC_T
Cuts at: 2287 4324 2287
Size: 2037 2735
BlpI GC'TnA_GC
Cuts at: 772 1632 1884 772
Size: 860 252 3660
Fragments arranged by size:
3660 860 252
Bme1580I G_kGCm'C
Cuts at: 1041 1138 2397 2490 3546 1041
Size: 97 1259 93 1056 2267
Fragments arranged by size:
2267 1259 1056 97 93
BmrI ACTGGGnnnn_n'
Cuts at: 1133 1984 2065 2400 4544 1133
Size: 851 81 335 2144 1361
Fragments arranged by size:
2144 1361 851 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 588 3139 588
Size: 2551 2221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1340 1340
Size: 4772
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1948 2827 3319 3617 1948
Size: 879 492 298 3103
Fragments arranged by size:
3103 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 940 4476 940
Size: 3536 1236
BsaAI yAC'GTr
Cuts at: 1179 2125 1179
Size: 946 3826
BsaBI GATnn'nnATC
Cuts at: 6 746 1420 2286 4323 6
Size: 740 674 866 2037 455
Fragments arranged by size:
2037 866 740 674 455
BsaHI Gr'CG_yC
Cuts at: 403 2203 2451 3153 4649 403
Size: 1800 248 702 1496 526
Fragments arranged by size:
1800 1496 702 526 248
BsaWI w'CCGG_w
Cuts at: 1961 2482 3160 3434 3581 3992 1961
Size: 521 678 274 147 411 2741
Fragments arranged by size:
2741 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 466
Size: 4772
BseYI C'CCAG_C
Cuts at: 1131 1499 1644 2197 2955 3532 4028 1131
Size: 368 145 553 758 577 496 1875
Fragments arranged by size:
1875 758 577 553 496 368 145
BsiEI CG_ry'CG
Cuts at: 104 159 2314 2360 3568 104
Size: 55 2155 46 1208 1308
Fragments arranged by size:
2155 1308 1208 55 46
BsiHKAI G_wGCw'C
Cuts at: 1786 2564 2754 3546 1786
Size: 778 190 792 3012
Fragments arranged by size:
3012 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 960 1074 1272 960
Size: 114 198 4460
Fragments arranged by size:
4460 198 114
Bsp1286I G_dGCh'C
Cuts at: 597 1041 1138 1786 2397 2490 2564 2754 2816
Size: 444 97 648 611 93 74 190 62
Cuts at: 2816 3546 597
Size: 730 1823
Fragments arranged by size:
1823 730 648 611 444 190 97 93
74 62
BspEI T'CCGG_A
Cuts at: 3992 3992
Size: 4772
BspHI T'CATG_A
Cuts at: 3948 3948
Size: 4772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1084 1822 2036 2338 2719 1084
Size: 738 214 302 381 3137
Fragments arranged by size:
3137 738 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3064 3118 4602 4767 50
Size: 3014 54 1484 165 55
Fragments arranged by size:
3014 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1407 1637 2684 1407
Size: 230 1047 3495
Fragments arranged by size:
3495 1047 230
BsrFI r'CCGG_y
Cuts at: 2770 2951 3160 2770
Size: 181 209 4382
Fragments arranged by size:
4382 209 181
BsrGI T'GTAC_A
Cuts at: 1697 1697
Size: 4772
BssSI C'ACGA_G
Cuts at: 3043 3401 3043
Size: 358 4414
BstAPI GCAn_nnn'nTGC
Cuts at: 1352 2149 1352
Size: 797 3975
BstBI TT'CG_AA
Cuts at: 3133 3133
Size: 4772
BstXI CCAn_nnnn'nTGG
Cuts at: 1125 1644 1716 1125
Size: 519 72 4181
Fragments arranged by size:
4181 519 72
BstYI r'GATC_y
Cuts at: 1 645 747 1104 1157 1623 1797 2264 2287
Size: 644 102 357 53 466 174 467 23
Cuts at: 2287 2622 2868 3880 3966 3978 4324 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 644 467 466 449 357 346 335
246 174 102 86 53 23 12
Bsu36I CC'TnA_GG
Cuts at: 376 376
Size: 4772
BtgI C'CryG_G
Cuts at: 107 1673 107
Size: 1566 3206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1378 2722 2903 3066 1378
Size: 1344 181 163 3084
Fragments arranged by size:
3084 1344 181 163
BtsI GCAGTG_nn'
Cuts at: 1001 2015 1001
Size: 1014 3758
DraI TTT'AAA
Cuts at: 708 1771 708
Size: 1063 3709
DraIII CAC_nnn'GTG
Cuts at: 984 984
Size: 4772
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2478 3336 1494
Size: 984 858 2930
Fragments arranged by size:
2930 984 858
EaeI y'GGCC_r
Cuts at: 156 172 686 698 1115 1451 1538 2357 2531
Size: 16 514 12 417 336 87 819 174
Cuts at: 2531 2922 2949 4294 156
Size: 391 27 1345 634
Fragments arranged by size:
1345 819 634 514 417 391 336 174
87 27 16 12
EagI C'GGCC_G
Cuts at: 156 2357 156
Size: 2201 2571
EarI CTCTTCn'nnn_
Cuts at: 362 535 564 830 2795 3005 362
Size: 173 29 266 1965 210 2129
Fragments arranged by size:
2129 1965 266 210 173 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1150 1731 2980 3290 3436 4582 1150
Size: 581 1249 310 146 1146 1340
Fragments arranged by size:
1340 1249 1146 581 310 146
EcoICRI GAG'CTC
Cuts at: 1784 1784
Size: 4772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 524 536 545 1082 1340 2597 3029 3776
Size: 217 12 9 537 258 1257 432 747
Cuts at: 3776 4044 307
Size: 268 1035
Fragments arranged by size:
1257 1035 747 537 432 268 258 217
12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 173 1480 1525 173
Size: 1307 45 3420
Fragments arranged by size:
3420 1307 45
EcoO109I rG'GnC_Cy
Cuts at: 1037 1122 1135 1911 1037
Size: 85 13 776 3898
Fragments arranged by size:
3898 776 85 13
EcoRI G'AATT_C
Cuts at: 1776 1776
Size: 4772
EcoRV GAT'ATC
Cuts at: 163 163
Size: 4772
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 246 1395 2791 246
Size: 1149 1396 2227
Fragments arranged by size:
2227 1396 1149
FspI TGC'GCA
Cuts at: 2553 2553
Size: 4772
HaeII r_GCGC'y
Cuts at: 1251 1717 2206 2454 3476 4455 1251
Size: 466 489 248 1022 979 1568
Fragments arranged by size:
1568 1022 979 489 466 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 174 206 388 420 2614 2644 2646 2676 174
Size: 32 182 32 2194 30 2 30 2270
Fragments arranged by size:
2270 2194 182 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1190 1659 1811 1190
Size: 469 152 4151
Fragments arranged by size:
4151 469 152
KasI G'GCGC_C
Cuts at: 2202 2450 2202
Size: 248 4524
KpnI G_GTAC'C
Cuts at: 1792 1792
Size: 4772
MluI A'CGCG_T
Cuts at: 4205 4205
Size: 4772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 983 1082 1690 3443 3627 983
Size: 99 608 1753 184 2128
Fragments arranged by size:
2128 1753 608 184 99
MscI TGG'CCA
Cuts at: 174 1453 1540 2533 174
Size: 1279 87 993 2413
Fragments arranged by size:
2413 1279 993 87
MslI CAynn'nnrTG
Cuts at: 880 2761 2888 880
Size: 1881 127 2764
Fragments arranged by size:
2764 1881 127
MspA1I CmG'CkG
Cuts at: 285 793 868 1060 1284 1352 1681 1883 2197
Size: 508 75 192 224 68 329 202 314
Cuts at: 2197 2557 3570 3815 285
Size: 360 1013 245 1242
Fragments arranged by size:
1242 1013 508 360 329 314 245 224
202 192 75 68
NaeI GCC'GGC
Cuts at: 2953 2953
Size: 4772
NarI GG'CG_CC
Cuts at: 2203 2451 2203
Size: 248 4524
NcoI C'CATG_G
Cuts at: 107 1673 107
Size: 1566 3206
NgoMIV G'CCGG_C
Cuts at: 2951 2951
Size: 4772
NspI r_CATG'y
Cuts at: 1188 1825 2325 3232 1188
Size: 637 500 907 2728
Fragments arranged by size:
2728 907 637 500
PasI CC'CwG_GG
Cuts at: 1165 1165
Size: 4772
PciI A'CATG_T
Cuts at: 1184 3228 1184
Size: 2044 2728
PflMI CCAn_nnn'nTGG
Cuts at: 1365 1365
Size: 4772
PfoI T'CCnGG_A
Cuts at: 656 1028 1550 656
Size: 372 522 3878
Fragments arranged by size:
3878 522 372
PmeI GTTT'AAAC
Cuts at: 1771 1771
Size: 4772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4740
PpuMI rG'GwC_Cy
Cuts at: 1122 1122
Size: 4772
PspOMI G'GGCC_C
Cuts at: 1037 1134 1037
Size: 97 4675
PstI C_TGCA'G
Cuts at: 1008 1348 1686 1819 2504 1008
Size: 340 338 133 685 3276
Fragments arranged by size:
3276 685 340 338 133
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4772
PvuII CAG'CTG
Cuts at: 285 793 868 1284 1352 1681 2197 2557 285
Size: 508 75 416 68 329 516 360 2500
Fragments arranged by size:
2500 516 508 416 360 329 75 68
RsrII CG'GwC_CG
Cuts at: 691 2967 691
Size: 2276 2496
SacI G_AGCT'C
Cuts at: 1786 1786
Size: 4772
SalI G'TCGA_C
Cuts at: 1809 1809
Size: 4772
SapI GCTCTTCn'nnn_
Cuts at: 564 2795 3005 564
Size: 2231 210 2331
Fragments arranged by size:
2331 2231 210
SbfI CC_TGCA'GG
Cuts at: 1819 1819
Size: 4772
ScaI AGT'ACT
Cuts at: 4289 4289
Size: 4772
SexAI A'CCwGG_T
Cuts at: 1124 1124
Size: 4772
SfcI C'TryA_G
Cuts at: 33 1004 1344 1682 1815 2500 3493 3684 33
Size: 971 340 338 133 685 993 191 1121
Fragments arranged by size:
1121 993 971 685 340 338 191 133
SfoI GGC'GCC
Cuts at: 2204 2452 2204
Size: 248 4524
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4772
SmaI CCC'GGG
Cuts at: 1794 1794
Size: 4772
SmlI C'TyrA_G
Cuts at: 1927 2842 3334 3596 1927
Size: 915 492 262 3103
Fragments arranged by size:
3103 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4772
SphI G_CATG'C
Cuts at: 1825 2325 1825
Size: 500 4272
StyI C'CwwG_G
Cuts at: 107 169 930 1109 1118 1386 1437 1673 1709
Size: 62 761 179 9 268 51 236 36
Cuts at: 1709 1906 2260 107
Size: 197 354 2619
Fragments arranged by size:
2619 761 354 268 236 197 179 62
51 36 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 708 915 1636 2355 2488 2947 3156 708
Size: 207 721 719 133 459 209 2324
Fragments arranged by size:
2324 721 719 459 209 207 133
TatI w'GTAC_w
Cuts at: 1697 4287 1697
Size: 2590 2182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1546 1578 4289 4321 1546
Size: 32 2711 32 1997
Fragments arranged by size:
2711 1997 32 32
Tth111I GACn'n_nGTC
Cuts at: 1207 2569 1207
Size: 1362 3410
XbaI T'CTAG_A
Cuts at: 1803 1803
Size: 4772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1548 2267 1548
Size: 719 4053
XmaI C'CCGG_G
Cuts at: 1792 1792
Size: 4772
XmnI GAAnn'nnTTC
Cuts at: 229 229
Size: 4772
ZraI GAC'GTC
Cuts at: 404 404
Size: 4772
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AloI AscI BaeI BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI
BsmI BssHII BstEII BstZ17I ClaI CspCI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI