Restriction map for
pF1KB3312
(Circular) (Six-base) MAPSORT of: pF1KB3312.seq Check: 8543 from: 1 to: 5585
pF1KB3312 5585 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2623 2623
Size: 5585
Acc65I G'GTAC_C
Cuts at: 2601 2601
Size: 5585
AclI AA'CG_TT
Cuts at: 5425 5425
Size: 5585
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1970 3410 3842 4195 4857 1970
Size: 1440 432 353 662 2698
Fragments arranged by size:
2698 1440 662 432 353
AfeI AGC'GCT
Cuts at: 2090 2090
Size: 5585
AflIII A'CryG_T
Cuts at: 474 2937 4737 5018 474
Size: 2463 1800 281 1041
Fragments arranged by size:
2463 1800 1041 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 343 661 343
Size: 318 5267
AlwNI CAG_nnn'CTG
Cuts at: 598 2890 4328 598
Size: 2292 1438 1855
Fragments arranged by size:
2292 1855 1438
ApaLI G'TGCA_C
Cuts at: 4423 4423
Size: 5585
ApoI r'AATT_y
Cuts at: 613 753 1800 1870 2056 2224 2382 2575 2589
Size: 140 1047 70 186 168 158 193 14
Cuts at: 2589 4938 5194 613
Size: 2349 256 1004
Fragments arranged by size:
2349 1047 1004 256 193 186 168 158
140 70 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 5585
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5585
AvaI C'yCGr_G
Cuts at: 2605 2605
Size: 5585
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 653 686 653
Size: 33 5552
BamHI G'GATC_C
Cuts at: 991 2610 991
Size: 1619 3966
BanI G'GyrC_C
Cuts at: 2601 3015 3263 3298 2601
Size: 414 248 35 4888
Fragments arranged by size:
4888 414 248 35
BanII G_rGCy'C
Cuts at: 1053 1201 2599 3629 1053
Size: 148 1398 1030 3009
Fragments arranged by size:
3009 1398 1030 148
BbeI G_GCGC'C
Cuts at: 3019 3267 3019
Size: 248 5337
BbsI GAAGACnn'nnnn_
Cuts at: 1095 1095
Size: 5585
BciVI GTATCCnnnnn_n'
Cuts at: 2169 3483 4539 5585 2169
Size: 1314 1056 1046 2169
Fragments arranged by size:
2169 1314 1056 1046
BclI T'GATC_A
Cuts at: 3105 3105
Size: 5585
BfrBI ATG'CAT
Cuts at: 2017 2076 2017
Size: 59 5526
BglII A'GATC_T
Cuts at: 1718 3100 5137 1718
Size: 1382 2037 2166
Fragments arranged by size:
2166 2037 1382
BlpI GC'TnA_GC
Cuts at: 2697 2697
Size: 5585
Bme1580I G_kGCm'C
Cuts at: 3210 3303 4427 3210
Size: 93 1124 4368
Fragments arranged by size:
4368 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 856 2797 2878 3213 5357 856
Size: 1941 81 335 2144 1084
Fragments arranged by size:
2144 1941 1084 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1740 3952 1740
Size: 2212 3373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1004
Size: 5585
Bpu10I CC'TnA_GC
Cuts at: 258 258
Size: 5585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2761 3640 4354 4652 2761
Size: 879 714 298 3694
Fragments arranged by size:
3694 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 560 5289 560
Size: 4729 856
BsaAI yAC'GTr
Cuts at: 2938 2938
Size: 5585
BsaBI GATnn'nnATC
Cuts at: 6 2405 3099 5136 6
Size: 2399 694 2037 455
Fragments arranged by size:
2399 2037 694 455
BsaHI Gr'CG_yC
Cuts at: 3016 3264 3966 5462 3016
Size: 248 702 1496 3139
Fragments arranged by size:
3139 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1045 2774 3295 3973 4384 4531 4805 1045
Size: 1729 521 678 411 147 274 1825
Fragments arranged by size:
1825 1729 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2485 2485
Size: 5585
BseYI C'CCAG_C
Cuts at: 3010 3768 4433 4841 3010
Size: 758 665 408 3754
Fragments arranged by size:
3754 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3127 3173 4403 104
Size: 3023 46 1230 1286
Fragments arranged by size:
3023 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2599 3377 3567 4427 2599
Size: 778 190 860 3757
Fragments arranged by size:
3757 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1053 1201 2599 3210 3303 3377 3567 3629 4427
Size: 148 1398 611 93 74 190 62 798
Cuts at: 4427 1053
Size: 2211
Fragments arranged by size:
2211 1398 798 611 190 148 93 74
62
BspHI T'CATG_A
Cuts at: 4017 4017
Size: 5585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 335 2635 2849 3151 3532 335
Size: 2300 214 302 381 2388
Fragments arranged by size:
2388 2300 381 302 214
BsrBI CCG'CTC
Cuts at: 50 257 3877 3931 5415 5580 50
Size: 207 3620 54 1484 165 55
Fragments arranged by size:
3620 1484 207 165 55 54
BsrDI GCAATG_nn'
Cuts at: 322 3497 322
Size: 3175 2410
BsrFI r'CCGG_y
Cuts at: 3583 3764 3583
Size: 181 5404
BssSI C'ACGA_G
Cuts at: 3856 4564 3856
Size: 708 4877
BstAPI GCAn_nnn'nTGC
Cuts at: 2962 2962
Size: 5585
BstBI TT'CG_AA
Cuts at: 1980 3946 1980
Size: 1966 3619
BstXI CCAn_nnnn'nTGG
Cuts at: 1814 1814
Size: 5585
BstYI r'GATC_y
Cuts at: 1 594 991 1718 2406 2610 3077 3100 3435
Size: 593 397 727 688 204 467 23 335
Cuts at: 3435 3681 3987 3999 4085 5137 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 727 688 593 467 449 397 335
306 246 204 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1187 1187
Size: 5585
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3535 3716 3879 3535
Size: 181 163 5241
Fragments arranged by size:
5241 181 163
BtsI GCAGTG_nn'
Cuts at: 1225 2828 1225
Size: 1603 3982
DraI TTT'AAA
Cuts at: 539 612 2584 539
Size: 73 1972 3540
Fragments arranged by size:
3540 1972 73
DraIII CAC_nnn'GTG
Cuts at: 1114 1114
Size: 5585
DrdI GACnn_nn'nnGTC
Cuts at: 3291 4635 3291
Size: 1344 4241
EaeI y'GGCC_r
Cuts at: 1080 3170 3344 3735 3762 5107 1080
Size: 2090 174 391 27 1345 1558
Fragments arranged by size:
2090 1558 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3170 3170
Size: 5585
EarI CTCTTCn'nnn_
Cuts at: 1413 3608 3818 1413
Size: 2195 210 3180
Fragments arranged by size:
3180 2195 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3793 4535 4681 5395 3793
Size: 742 146 714 3983
Fragments arranged by size:
3983 742 714 146
EcoICRI GAG'CTC
Cuts at: 2597 2597
Size: 5585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1970 3410 3842 4195 4857 1004
Size: 966 1440 432 353 662 1732
Fragments arranged by size:
1732 1440 966 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1628 2724 1628
Size: 1096 4489
EcoRI G'AATT_C
Cuts at: 2589 2589
Size: 5585
EcoRV GAT'ATC
Cuts at: 2097 2097
Size: 5585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 798 1119 3604 798
Size: 321 2485 2779
Fragments arranged by size:
2779 2485 321
FspI TGC'GCA
Cuts at: 3366 3366
Size: 5585
HaeII r_GCGC'y
Cuts at: 2092 3019 3267 4497 5268 2092
Size: 927 248 1230 771 2409
Fragments arranged by size:
2409 1230 927 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1024 1056 3427 3457 3459 3489 1024
Size: 32 2371 30 2 30 3120
Fragments arranged by size:
3120 2371 32 30 30 2
HincII GTy'rAC
Cuts at: 2624 2624
Size: 5585
HindIII A'AGCT_T
Cuts at: 358 358
Size: 5585
KasI G'GCGC_C
Cuts at: 3015 3263 3015
Size: 248 5337
KpnI G_GTAC'C
Cuts at: 2605 2605
Size: 5585
MfeI C'AATT_G
Cuts at: 734 2441 734
Size: 1707 3878
MluI A'CGCG_T
Cuts at: 5018 5018
Size: 5585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4344 4528 4344
Size: 184 5401
MscI TGG'CCA
Cuts at: 3346 3346
Size: 5585
MslI CAynn'nnrTG
Cuts at: 112 315 2012 3574 3701 112
Size: 203 1697 1562 127 1996
Fragments arranged by size:
1996 1697 1562 203 127
MspA1I CmG'CkG
Cuts at: 820 2563 2696 3010 3370 4154 4399 820
Size: 1743 133 314 360 784 245 2006
Fragments arranged by size:
2006 1743 784 360 314 245 133
NaeI GCC'GGC
Cuts at: 3766 3766
Size: 5585
NarI GG'CG_CC
Cuts at: 3016 3264 3016
Size: 248 5337
NgoMIV G'CCGG_C
Cuts at: 3764 3764
Size: 5585
NsiI A_TGCA'T
Cuts at: 2019 2078 2019
Size: 59 5526
NspI r_CATG'y
Cuts at: 478 1656 2149 2638 3138 4741 478
Size: 1178 493 489 500 1603 1322
Fragments arranged by size:
1603 1322 1178 500 493 489
PciI A'CATG_T
Cuts at: 474 4737 474
Size: 4263 1322
PflMI CCAn_nnn'nTGG
Cuts at: 898 952 982 898
Size: 54 30 5501
Fragments arranged by size:
5501 54 30
PfoI T'CCnGG_A
Cuts at: 981 981
Size: 5585
PmeI GTTT'AAAC
Cuts at: 2584 2584
Size: 5585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 5553
PsiI TTA'TAA
Cuts at: 2012 2012
Size: 5585
PstI C_TGCA'G
Cuts at: 122 1827 2632 3317 122
Size: 1705 805 685 2390
Fragments arranged by size:
2390 1705 805 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5585
PvuII CAG'CTG
Cuts at: 820 2563 3010 3370 820
Size: 1743 447 360 3035
Fragments arranged by size:
3035 1743 447 360
RsrII CG'GwC_CG
Cuts at: 3780 3780
Size: 5585
SacI G_AGCT'C
Cuts at: 2599 2599
Size: 5585
SalI G'TCGA_C
Cuts at: 2622 2622
Size: 5585
SapI GCTCTTCn'nnn_
Cuts at: 3608 3818 3608
Size: 210 5375
SbfI CC_TGCA'GG
Cuts at: 2632 2632
Size: 5585
ScaI AGT'ACT
Cuts at: 778 5102 778
Size: 4324 1261
SfcI C'TryA_G
Cuts at: 33 118 1823 2628 3313 4281 4472 33
Size: 85 1705 805 685 968 191 1146
Fragments arranged by size:
1705 1146 968 805 685 191 85
SfoI GGC'GCC
Cuts at: 3017 3265 3017
Size: 248 5337
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5585
SmaI CCC'GGG
Cuts at: 2607 2607
Size: 5585
SmlI C'TyrA_G
Cuts at: 2740 3655 4369 4631 2740
Size: 915 714 262 3694
Fragments arranged by size:
3694 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1255 67
Size: 1188 4397
SphI G_CATG'C
Cuts at: 2638 3138 2638
Size: 500 5085
SspI AAT'ATT
Cuts at: 1566 1670 1566
Size: 104 5481
StuI AGG'CCT
Cuts at: 1459 1813 1459
Size: 354 5231
StyI C'CwwG_G
Cuts at: 1274 2719 3073 1274
Size: 1445 354 3786
Fragments arranged by size:
3786 1445 354
SwaI ATTT'AAAT
Cuts at: 612 612
Size: 5585
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3168 3301 3760 3969 3168
Size: 133 459 209 4784
Fragments arranged by size:
4784 459 209 133
TatI w'GTAC_w
Cuts at: 210 304 776 1177 1649 5100 210
Size: 94 472 401 472 3451 695
Fragments arranged by size:
3451 695 472 472 401 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1301 1333 5102 5134 1301
Size: 32 3769 32 1752
Fragments arranged by size:
3769 1752 32 32
Tth111I GACn'n_nGTC
Cuts at: 3382 3382
Size: 5585
XbaI T'CTAG_A
Cuts at: 2616 2616
Size: 5585
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1597 3080 1597
Size: 1483 4102
XmaI C'CCGG_G
Cuts at: 2605 2605
Size: 5585
XmnI GAAnn'nnTTC
Cuts at: 1239 1834 1239
Size: 595 4990
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BbvCI BcgI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I BtgI ClaI CspCI EcoNI FseI FspAI HpaI
NcoI NdeI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI XhoI ZraI