Restriction map for
pF1KB3311
(Circular) (Six-base) MAPSORT of: pF1KB3311.seq Check: 8942 from: 1 to: 4991
pF1KB3311 4991 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 1128 1128
Size: 4991
AccI GT'mk_AC
Cuts at: 1115 2029 1115
Size: 914 4077
Acc65I G'GTAC_C
Cuts at: 2007 2007
Size: 4991
AclI AA'CG_TT
Cuts at: 4831 4831
Size: 4991
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1595 1603 1939 2816 3248 3601 4263 1595
Size: 8 336 877 432 353 662 2323
Fragments arranged by size:
2323 877 662 432 353 336 8
AflIII A'CryG_T
Cuts at: 979 2343 4143 4424 979
Size: 1364 1800 281 1546
Fragments arranged by size:
1800 1546 1364 281
AhdI GACnn_n'nnGTC
Cuts at: 1935 1935
Size: 4991
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1091 1091
Size: 4991
AlwNI CAG_nnn'CTG
Cuts at: 1364 1399 1457 1686 1833 2296 3734 1364
Size: 35 58 229 147 463 1438 2621
Fragments arranged by size:
2621 1438 463 229 147 58 35
ApaLI G'TGCA_C
Cuts at: 3829 3829
Size: 4991
ApoI r'AATT_y
Cuts at: 1674 1995 4344 4600 1674
Size: 321 2349 256 2065
Fragments arranged by size:
2349 2065 321 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4991
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
AvaI C'yCGr_G
Cuts at: 303 2011 303
Size: 1708 3283
BamHI G'GATC_C
Cuts at: 2016 2016
Size: 4991
BanI G'GyrC_C
Cuts at: 378 594 1861 2007 2421 2669 2704 378
Size: 216 1267 146 414 248 35 2665
Fragments arranged by size:
2665 1267 414 248 216 146 35
BanII G_rGCy'C
Cuts at: 961 1249 1841 2005 3035 961
Size: 288 592 164 1030 2917
Fragments arranged by size:
2917 1030 592 288 164
BbeI G_GCGC'C
Cuts at: 382 598 2425 2673 382
Size: 216 1827 248 2700
Fragments arranged by size:
2700 1827 248 216
BbsI GAAGACnn'nnnn_
Cuts at: 1934 1934
Size: 4991
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 572 606 1604 1638 90
Size: 34 448 34 998 34 3443
Fragments arranged by size:
3443 998 448 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1458 2889 3945 4991 1458
Size: 1431 1056 1046 1458
Fragments arranged by size:
1458 1431 1056 1046
BclI T'GATC_A
Cuts at: 356 1733 2511 356
Size: 1377 778 2836
Fragments arranged by size:
2836 1377 778
BglI GCCn_nnn'nGGC
Cuts at: 725 856 868 920 725
Size: 131 12 52 4796
Fragments arranged by size:
4796 131 52 12
BglII A'GATC_T
Cuts at: 1344 1883 2506 4543 1344
Size: 539 623 2037 1792
Fragments arranged by size:
2037 1792 623 539
BlpI GC'TnA_GC
Cuts at: 769 1400 2103 769
Size: 631 703 3657
Fragments arranged by size:
3657 703 631
Bme1580I G_kGCm'C
Cuts at: 979 1866 2616 2709 3833 979
Size: 887 750 93 1124 2137
Fragments arranged by size:
2137 1124 887 750 93
BmgBI CAC'GTC
Cuts at: 266 266
Size: 4991
BmrI ACTGGGnnnn_n'
Cuts at: 1105 2203 2284 2619 4763 1105
Size: 1098 81 335 2144 1333
Fragments arranged by size:
2144 1333 1098 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 318 3358 318
Size: 3040 1951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 446 453 1855 1885 128
Size: 318 7 1402 30 3234
Fragments arranged by size:
3234 1402 318 30 7
Bpu10I CC'TnA_GC
Cuts at: 1707 1707
Size: 4991
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 841 2167 3046 3760 4058 304
Size: 537 1326 879 714 298 1237
Fragments arranged by size:
1326 1237 879 714 537 298
BsaI GGTCTCn'nnnn_
Cuts at: 4695 4695
Size: 4991
BsaAI yAC'GTr
Cuts at: 980 2344 980
Size: 1364 3627
BsaBI GATnn'nnATC
Cuts at: 6 2505 4542 6
Size: 2499 2037 455
Fragments arranged by size:
2499 2037 455
BsaHI Gr'CG_yC
Cuts at: 379 595 1125 2422 2670 3372 4868 379
Size: 216 530 1297 248 702 1496 502
Fragments arranged by size:
1496 1297 702 530 502 248 216
BsaWI w'CCGG_w
Cuts at: 2180 2701 3379 3790 3937 4211 2180
Size: 521 678 411 147 274 2960
Fragments arranged by size:
2960 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1206 1209 1236 1239 1206
Size: 3 27 3 4958
Fragments arranged by size:
4958 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 1230 1277 382
Size: 848 47 4096
Fragments arranged by size:
4096 848 47
BseYI C'CCAG_C
Cuts at: 274 382 493 715 780 918 931 949 1457
Size: 108 111 222 65 138 13 18 508
Cuts at: 1457 2416 3174 3839 4247 274
Size: 959 758 665 408 1018
Fragments arranged by size:
1018 959 758 665 508 408 222 138
111 108 65 18 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1872 1087
Size: 785 4206
BsiEI CG_ry'CG
Cuts at: 104 725 758 2533 2579 3809 104
Size: 621 33 1775 46 1230 1286
Fragments arranged by size:
1775 1286 1230 621 46 33
BsiHKAI G_wGCw'C
Cuts at: 961 2005 2783 2973 3833 961
Size: 1044 778 190 860 2119
Fragments arranged by size:
2119 1044 860 778 190
BsiWI C'GTAC_G
Cuts at: 833 833
Size: 4991
BsmI GAATG_Cn'
Cuts at: 1311 1311
Size: 4991
BsmBI CGTCTCn'nnnn_
Cuts at: 1116 1116
Size: 4991
Bsp1286I G_dGCh'C
Cuts at: 961 979 1249 1841 1866 2005 2616 2709 2783
Size: 18 270 592 25 139 611 93 74
Cuts at: 2783 2973 3035 3833 961
Size: 190 62 798 2119
Fragments arranged by size:
2119 798 611 592 270 190 139 93
74 62 25 18
BspHI T'CATG_A
Cuts at: 197 3423 197
Size: 3226 1765
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1039 2041 2255 2557 2938 1039
Size: 1002 214 302 381 3092
Fragments arranged by size:
3092 1002 381 302 214
BsrBI CCG'CTC
Cuts at: 50 675 3283 3337 4821 4986 50
Size: 625 2608 54 1484 165 55
Fragments arranged by size:
2608 1484 625 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1602 2903 1602
Size: 1301 3690
BsrFI r'CCGG_y
Cuts at: 543 658 724 2989 3170 543
Size: 115 66 2265 181 2364
Fragments arranged by size:
2364 2265 181 115 66
BssHII G'CGCG_C
Cuts at: 662 664 662
Size: 2 4989
BssSI C'ACGA_G
Cuts at: 3262 3970 3262
Size: 708 4283
BstAPI GCAn_nnn'nTGC
Cuts at: 2368 2368
Size: 4991
BstBI TT'CG_AA
Cuts at: 3352 3352
Size: 4991
BstXI CCAn_nnnn'nTGG
Cuts at: 918 1857 918
Size: 939 4052
BstYI r'GATC_y
Cuts at: 1 1344 1883 2016 2483 2506 2841 3087 3393
Size: 1343 539 133 467 23 335 246 306
Cuts at: 3393 3405 3491 4543 1
Size: 12 86 1052 449
Fragments arranged by size:
1343 1052 539 467 449 335 306 246
133 86 23 12
BtgI C'CryG_G
Cuts at: 188 224 650 797 188
Size: 36 426 147 4382
Fragments arranged by size:
4382 426 147 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 591 2941 3122 3285 591
Size: 2350 181 163 2297
Fragments arranged by size:
2350 2297 181 163
BtsI GCAGTG_nn'
Cuts at: 583 1568 2234 583
Size: 985 666 3340
Fragments arranged by size:
3340 985 666
DraI TTT'AAA
Cuts at: 1805 1990 1805
Size: 185 4806
DrdI GACnn_nn'nnGTC
Cuts at: 2697 4041 2697
Size: 1344 3647
EaeI y'GGCC_r
Cuts at: 656 722 729 755 848 2576 2750 3141 3168
Size: 66 7 26 93 1728 174 391 27
Cuts at: 3168 4513 656
Size: 1345 1134
Fragments arranged by size:
1728 1345 1134 391 174 93 66 27
26 7
EagI C'GGCC_G
Cuts at: 722 755 2576 722
Size: 33 1821 3137
Fragments arranged by size:
3137 1821 33
EarI CTCTTCn'nnn_
Cuts at: 682 1769 1969 3014 3224 682
Size: 1087 200 1045 210 2449
Fragments arranged by size:
2449 1087 1045 210 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3199 3941 4087 4801 3199
Size: 742 146 714 3389
Fragments arranged by size:
3389 742 714 146
EcoICRI GAG'CTC
Cuts at: 959 2003 959
Size: 1044 3947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 446 453 1595 1603 1855 1885 1939 2816
Size: 318 7 1142 8 252 30 54 877
Cuts at: 2816 3248 3601 4263 128
Size: 432 353 662 856
Fragments arranged by size:
1142 877 856 662 432 353 318 252
54 30 8 7
EcoO109I rG'GnC_Cy
Cuts at: 1854 2130 1854
Size: 276 4715
EcoRI G'AATT_C
Cuts at: 1995 1995
Size: 4991
EcoRV GAT'ATC
Cuts at: 1698 1698
Size: 4991
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3010 3010
Size: 4991
FspI TGC'GCA
Cuts at: 2772 2772
Size: 4991
HaeII r_GCGC'y
Cuts at: 382 598 2425 2673 3903 4674 382
Size: 216 1827 248 1230 771 699
Fragments arranged by size:
1827 1230 771 699 248 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1206 1209 1238 1241 1293 1325 1541 1573 1921
Size: 3 29 3 52 32 216 32 348
Cuts at: 1921 1953 2833 2863 2865 2895 1206
Size: 32 880 30 2 30 3302
Fragments arranged by size:
3302 880 348 216 52 32 32 32
30 30 29 3 3 2
HincII GTy'rAC
Cuts at: 2030 2030
Size: 4991
KasI G'GCGC_C
Cuts at: 378 594 2421 2669 378
Size: 216 1827 248 2700
Fragments arranged by size:
2700 1827 248 216
KpnI G_GTAC'C
Cuts at: 2011 2011
Size: 4991
MluI A'CGCG_T
Cuts at: 4424 4424
Size: 4991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1152 3750 3934 1152
Size: 2598 184 2209
Fragments arranged by size:
2598 2209 184
MscI TGG'CCA
Cuts at: 850 2752 850
Size: 1902 3089
MslI CAynn'nnrTG
Cuts at: 502 2980 3107 502
Size: 2478 127 2386
Fragments arranged by size:
2478 2386 127
MspA1I CmG'CkG
Cuts at: 348 621 760 780 799 846 867 2102 2416
Size: 273 139 20 19 47 21 1235 314
Cuts at: 2416 2776 3560 3805 348
Size: 360 784 245 1534
Fragments arranged by size:
1534 1235 784 360 314 273 245 139
47 21 20 19
NaeI GCC'GGC
Cuts at: 545 660 726 3172 545
Size: 115 66 2446 2364
Fragments arranged by size:
2446 2364 115 66
NarI GG'CG_CC
Cuts at: 379 595 2422 2670 379
Size: 216 1827 248 2700
Fragments arranged by size:
2700 1827 248 216
NcoI C'CATG_G
Cuts at: 224 224
Size: 4991
NgoMIV G'CCGG_C
Cuts at: 543 658 724 3170 543
Size: 115 66 2446 2364
Fragments arranged by size:
2446 2364 115 66
NspI r_CATG'y
Cuts at: 1408 2044 2544 4147 1408
Size: 636 500 1603 2252
Fragments arranged by size:
2252 1603 636 500
PasI CC'CwG_GG
Cuts at: 1893 1893
Size: 4991
PciI A'CATG_T
Cuts at: 4143 4143
Size: 4991
PflMI CCAn_nnn'nTGG
Cuts at: 1457 1457
Size: 4991
PfoI T'CCnGG_A
Cuts at: 1473 1967 1473
Size: 494 4497
PmeI GTTT'AAAC
Cuts at: 1990 1990
Size: 4991
PmlI CAC'GTG
Cuts at: 980 980
Size: 4991
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 4959
PpuMI rG'GwC_Cy
Cuts at: 1854 1854
Size: 4991
PspXI vC'TCGA_Gb
Cuts at: 303 303
Size: 4991
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 193 225 193
Size: 32 4959
PstI C_TGCA'G
Cuts at: 1389 2038 2723 1389
Size: 649 685 3657
Fragments arranged by size:
3657 685 649
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4991
PvuII CAG'CTG
Cuts at: 2416 2776 2416
Size: 360 4631
RsrII CG'GwC_CG
Cuts at: 3186 3186
Size: 4991
SacI G_AGCT'C
Cuts at: 961 2005 961
Size: 1044 3947
SacII CC_GC'GG
Cuts at: 800 800
Size: 4991
SalI G'TCGA_C
Cuts at: 2028 2028
Size: 4991
SapI GCTCTTCn'nnn_
Cuts at: 682 1769 1969 3014 3224 682
Size: 1087 200 1045 210 2449
Fragments arranged by size:
2449 1087 1045 210 200
SbfI CC_TGCA'GG
Cuts at: 2038 2038
Size: 4991
ScaI AGT'ACT
Cuts at: 1144 4508 1144
Size: 3364 1627
SfcI C'TryA_G
Cuts at: 33 254 1385 2034 2719 3687 3878 33
Size: 221 1131 649 685 968 191 1146
Fragments arranged by size:
1146 1131 968 685 649 221 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 868 868
Size: 4991
SfoI GGC'GCC
Cuts at: 380 596 2423 2671 380
Size: 216 1827 248 2700
Fragments arranged by size:
2700 1827 248 216
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
SmaI CCC'GGG
Cuts at: 2013 2013
Size: 4991
SmlI C'TyrA_G
Cuts at: 303 319 820 2146 3061 3775 4037 303
Size: 16 501 1326 915 714 262 1257
Fragments arranged by size:
1326 1257 915 714 501 262 16
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4991
SphI G_CATG'C
Cuts at: 2044 2544 2044
Size: 500 4491
StyI C'CwwG_G
Cuts at: 224 231 1850 2125 2479 224
Size: 7 1619 275 354 2736
Fragments arranged by size:
2736 1619 354 275 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1353 1849 2574 2707 3166 3375 1353
Size: 496 725 133 459 209 2969
Fragments arranged by size:
2969 725 496 459 209 133
TatI w'GTAC_w
Cuts at: 506 1142 4506 506
Size: 636 3364 991
Fragments arranged by size:
3364 991 636
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4508 4540 4508
Size: 32 4959
Tth111I GACn'n_nGTC
Cuts at: 2788 2788
Size: 4991
XbaI T'CTAG_A
Cuts at: 2022 2022
Size: 4991
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 222 2486 222
Size: 2264 2727
XhoI C'TCGA_G
Cuts at: 303 303
Size: 4991
XmaI C'CCGG_G
Cuts at: 2011 2011
Size: 4991
XmnI GAAnn'nnTTC
Cuts at: 1469 1469
Size: 4991
ZraI GAC'GTC
Cuts at: 1126 1126
Size: 4991
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI BspEI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspOMI SanDI SexAI SgrAI SnaBI
SrfI SspI StuI SwaI