Restriction map for
pF1KB3294
(Circular) (Six-base) MAPSORT of: pF1KB3294.seq Check: 726 from: 1 to: 5963
pF1KB3294 5963 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1248 1752 1248
Size: 504 5459
AccI GT'mk_AC
Cuts at: 845 3001 845
Size: 2156 3807
Acc65I G'GTAC_C
Cuts at: 2979 2979
Size: 5963
AclI AA'CG_TT
Cuts at: 5803 5803
Size: 5963
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 489 2406 3788 4220 4573 5235 489
Size: 1917 1382 432 353 662 1217
Fragments arranged by size:
1917 1382 1217 662 432 353
AflIII A'CryG_T
Cuts at: 1628 1919 2198 2450 3315 5115 5396 1628
Size: 291 279 252 865 1800 281 2195
Fragments arranged by size:
2195 1800 865 291 281 279 252
AleI CACnn'nnGTG
Cuts at: 2323 2323
Size: 5963
AlwNI CAG_nnn'CTG
Cuts at: 261 1674 3268 4706 261
Size: 1413 1594 1438 1518
Fragments arranged by size:
1594 1518 1438 1413
ApaI G_GGCC'C
Cuts at: 2173 2398 2173
Size: 225 5738
ApaLI G'TGCA_C
Cuts at: 1636 2566 4801 1636
Size: 930 2235 2798
Fragments arranged by size:
2798 2235 930
ApoI r'AATT_y
Cuts at: 366 552 982 1058 2481 2767 2967 5316 5572
Size: 186 430 76 1423 286 200 2349 256
Cuts at: 5572 366
Size: 757
Fragments arranged by size:
2349 1423 757 430 286 256 200 186
76
AseI AT'TA_AT
Cuts at: 20 20
Size: 5963
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5963
AvaI C'yCGr_G
Cuts at: 140 627 1480 1811 1908 2983 140
Size: 487 853 331 97 1075 3120
Fragments arranged by size:
3120 1075 853 487 331 97
BamHI G'GATC_C
Cuts at: 2988 2988
Size: 5963
BanI G'GyrC_C
Cuts at: 232 285 1246 1806 1848 2130 2979 3393 3641
Size: 53 961 560 42 282 849 414 248
Cuts at: 3641 3676 232
Size: 35 2519
Fragments arranged by size:
2519 961 849 560 414 282 248 53
42 35
BanII G_rGCy'C
Cuts at: 141 1402 2173 2398 2977 4007 141
Size: 1261 771 225 579 1030 2097
Fragments arranged by size:
2097 1261 1030 771 579 225
BbeI G_GCGC'C
Cuts at: 1810 2134 3397 3645 1810
Size: 324 1263 248 4128
Fragments arranged by size:
4128 1263 324 248
BbsI GAAGACnn'nnnn_
Cuts at: 495 2135 495
Size: 1640 4323
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1194 1228 1194
Size: 34 5929
BciVI GTATCCnnnnn_n'
Cuts at: 2643 3861 4917 5963 2643
Size: 1218 1056 1046 2643
Fragments arranged by size:
2643 1218 1056 1046
BclI T'GATC_A
Cuts at: 794 2207 2613 3483 794
Size: 1413 406 870 3274
Fragments arranged by size:
3274 1413 870 406
BglII A'GATC_T
Cuts at: 3478 5515 3478
Size: 2037 3926
BlpI GC'TnA_GC
Cuts at: 622 3075 622
Size: 2453 3510
Bme1580I G_kGCm'C
Cuts at: 1640 2173 2398 2570 3588 3681 4805 1640
Size: 533 225 172 1018 93 1124 2798
Fragments arranged by size:
2798 1124 1018 533 225 172 93
BmgBI CAC'GTC
Cuts at: 1284 1449 2453 1284
Size: 165 1004 4794
Fragments arranged by size:
4794 1004 165
BmrI ACTGGGnnnn_n'
Cuts at: 3175 3256 3591 5735 3175
Size: 81 335 2144 3403
Fragments arranged by size:
3403 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 695 4330 695
Size: 3635 2328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 352 445 1133 1711 2316 2660 289
Size: 63 93 688 578 605 344 3592
Fragments arranged by size:
3592 688 605 578 344 93 63
Bpu10I CC'TnA_GC
Cuts at: 254 1002 254
Size: 748 5215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3139 4018 4732 5030 3139
Size: 879 714 298 4072
Fragments arranged by size:
4072 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2863 5667 2863
Size: 2804 3159
BsaAI yAC'GTr
Cuts at: 1629 3316 1629
Size: 1687 4276
BsaBI GATnn'nnATC
Cuts at: 6 3477 5514 6
Size: 3471 2037 455
Fragments arranged by size:
3471 2037 455
BsaHI Gr'CG_yC
Cuts at: 307 1807 1901 2131 2140 2152 3394 3642 4344
Size: 1500 94 230 9 12 1242 248 702
Cuts at: 4344 5840 307
Size: 1496 430
Fragments arranged by size:
1500 1496 1242 702 430 248 230 94
12 9
BsaWI w'CCGG_w
Cuts at: 765 2743 3152 3673 4351 4762 4909 5183 765
Size: 1978 409 521 678 411 147 274 1545
Fragments arranged by size:
1978 1545 678 521 411 409 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2521 2551 2521
Size: 30 5933
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 400 1699 2590 2881 157
Size: 243 1299 891 291 3239
Fragments arranged by size:
3239 1299 891 291 243
BseYI C'CCAG_C
Cuts at: 3388 4146 4811 5219 3388
Size: 758 665 408 4132
Fragments arranged by size:
4132 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 1853 432
Size: 1421 4542
BsiEI CG_ry'CG
Cuts at: 104 381 3505 3551 4781 104
Size: 277 3124 46 1230 1286
Fragments arranged by size:
3124 1286 1230 277 46
BsiHKAI G_wGCw'C
Cuts at: 1640 2246 2278 2332 2386 2570 2977 3755 3945
Size: 606 32 54 54 184 407 778 190
Cuts at: 3945 4805 1640
Size: 860 2798
Fragments arranged by size:
2798 860 778 606 407 190 184 54
54 32
BsmI GAATG_Cn'
Cuts at: 2585 2585
Size: 5963
Bsp1286I G_dGCh'C
Cuts at: 141 1402 1640 2173 2246 2278 2332 2386 2398
Size: 1261 238 533 73 32 54 54 12
Cuts at: 2398 2570 2977 3588 3681 3755 3945 4007 4805
Size: 172 407 611 93 74 190 62 798
Cuts at: 4805 141
Size: 1299
Fragments arranged by size:
1299 1261 798 611 533 407 238 190
172 93 74 73 62 54 54 32
12
BspHI T'CATG_A
Cuts at: 4395 4395
Size: 5963
BspMI ACCTGCnnnn'nnnn_
Cuts at: 441 1248 1752 3013 3227 3529 3910 441
Size: 807 504 1261 214 302 381 2494
Fragments arranged by size:
2494 1261 807 504 381 302 214
BsrBI CCG'CTC
Cuts at: 50 191 1649 2123 4255 4309 5793 5958 50
Size: 141 1458 474 2132 54 1484 165 55
Fragments arranged by size:
2132 1484 1458 474 165 141 55 54
BsrDI GCAATG_nn'
Cuts at: 3875 3875
Size: 5963
BsrFI r'CCGG_y
Cuts at: 1794 1944 2076 3961 4142 1794
Size: 150 132 1885 181 3615
Fragments arranged by size:
3615 1885 181 150 132
BsrGI T'GTAC_A
Cuts at: 1985 2258 1985
Size: 273 5690
BssHII G'CGCG_C
Cuts at: 1611 1613 1715 1890 1958 1960 1611
Size: 2 102 175 68 2 5614
Fragments arranged by size:
5614 175 102 68 2 2
BssSI C'ACGA_G
Cuts at: 965 1681 2009 4234 4942 965
Size: 716 328 2225 708 1986
Fragments arranged by size:
2225 1986 716 708 328
BstAPI GCAn_nnn'nTGC
Cuts at: 3340 3340
Size: 5963
BstBI TT'CG_AA
Cuts at: 4324 4324
Size: 5963
BstEII G'GTnAC_C
Cuts at: 1380 1380
Size: 5963
BstXI CCAn_nnnn'nTGG
Cuts at: 2238 2340 2238
Size: 102 5861
BstYI r'GATC_y
Cuts at: 1 144 2487 2782 2988 3455 3478 3813 4059
Size: 143 2343 295 206 467 23 335 246
Cuts at: 4059 4365 4377 4463 5515 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2343 1052 467 449 335 306 295 246
206 143 86 23 12
BstZ17I GTA'TAC
Cuts at: 846 846
Size: 5963
BtgI C'CryG_G
Cuts at: 107 1418 1433 2090 107
Size: 1311 15 657 3980
Fragments arranged by size:
3980 1311 657 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1197 2196 3913 4094 4257 1197
Size: 999 1717 181 163 2903
Fragments arranged by size:
2903 1717 999 181 163
BtsI GCAGTG_nn'
Cuts at: 1663 1901 2248 2626 3206 1663
Size: 238 347 378 580 4420
Fragments arranged by size:
4420 580 378 347 238
ClaI AT'CG_AT
Cuts at: 377 377
Size: 5963
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2085 2120 2085
Size: 35 5928
DraI TTT'AAA
Cuts at: 2715 2962 2715
Size: 247 5716
DrdI GACnn_nn'nnGTC
Cuts at: 3669 5013 3669
Size: 1344 4619
EaeI y'GGCC_r
Cuts at: 192 243 1421 1442 2093 2124 2623 3548 3722
Size: 51 1178 21 651 31 499 925 174
Cuts at: 3722 4113 4140 5485 192
Size: 391 27 1345 670
Fragments arranged by size:
1345 1178 925 670 651 499 391 174
51 31 27 21
EagI C'GGCC_G
Cuts at: 3548 3548
Size: 5963
EarI CTCTTCn'nnn_
Cuts at: 611 3986 4196 611
Size: 3375 210 2378
Fragments arranged by size:
3375 2378 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 292 4171 4913 5059 5773 292
Size: 3879 742 146 714 482
Fragments arranged by size:
3879 742 714 482 146
EcoICRI GAG'CTC
Cuts at: 2975 2975
Size: 5963
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 352 445 489 1133 1711 2316 2406 2660
Size: 63 93 44 644 578 605 90 254
Cuts at: 2660 3788 4220 4573 5235 289
Size: 1128 432 353 662 1017
Fragments arranged by size:
1128 1017 662 644 605 578 432 353
254 93 90 63 44
EcoNI CCTnn'n_nnAGG
Cuts at: 331 2020 2575 331
Size: 1689 555 3719
Fragments arranged by size:
3719 1689 555
EcoO109I rG'GnC_Cy
Cuts at: 129 258 324 2394 2608 3102 129
Size: 129 66 2070 214 494 2990
Fragments arranged by size:
2990 2070 494 214 129 66
EcoRI G'AATT_C
Cuts at: 366 552 2967 366
Size: 186 2415 3362
Fragments arranged by size:
3362 2415 186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3982 3982
Size: 5963
FspI TGC'GCA
Cuts at: 3744 3744
Size: 5963
HaeII r_GCGC'y
Cuts at: 1343 1801 1810 2083 2134 2185 2269 3397 3645
Size: 458 9 273 51 51 84 1128 248
Cuts at: 3645 4875 5646 1343
Size: 1230 771 1660
Fragments arranged by size:
1660 1230 1128 771 458 273 248 84
51 51 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 625 657 985 1017 1086 1118 1225 1257 1345
Size: 32 328 32 69 32 107 32 88
Cuts at: 1345 1377 1384 1416 1891 1923 3805 3835 3837
Size: 32 7 32 475 32 1882 30 2
Cuts at: 3837 3867 625
Size: 30 2721
Fragments arranged by size:
2721 1882 475 328 107 88 69 32
32 32 32 32 32 32 30 30
7 2
HincII GTy'rAC
Cuts at: 2196 2694 3002 2196
Size: 498 308 5157
Fragments arranged by size:
5157 498 308
KasI G'GCGC_C
Cuts at: 1806 2130 3393 3641 1806
Size: 324 1263 248 4128
Fragments arranged by size:
4128 1263 324 248
KpnI G_GTAC'C
Cuts at: 2983 2983
Size: 5963
MluI A'CGCG_T
Cuts at: 1919 5396 1919
Size: 3477 2486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1313 4722 4906 1313
Size: 3409 184 2370
Fragments arranged by size:
3409 2370 184
MscI TGG'CCA
Cuts at: 2625 3724 2625
Size: 1099 4864
MslI CAynn'nnrTG
Cuts at: 1225 2323 3952 4079 1225
Size: 1098 1629 127 3109
Fragments arranged by size:
3109 1629 1098 127
MspA1I CmG'CkG
Cuts at: 447 2705 3074 3388 3748 4532 4777 447
Size: 2258 369 314 360 784 245 1633
Fragments arranged by size:
2258 1633 784 369 360 314 245
NaeI GCC'GGC
Cuts at: 1796 1946 2078 4144 1796
Size: 150 132 2066 3615
Fragments arranged by size:
3615 2066 150 132
NarI GG'CG_CC
Cuts at: 1807 2131 3394 3642 1807
Size: 324 1263 248 4128
Fragments arranged by size:
4128 1263 324 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5963
NgoMIV G'CCGG_C
Cuts at: 1794 1944 2076 4142 1794
Size: 150 132 2066 3615
Fragments arranged by size:
3615 2066 150 132
NruI TCG'CGA
Cuts at: 383 1352 383
Size: 969 4994
NspI r_CATG'y
Cuts at: 2556 3016 3516 5119 2556
Size: 460 500 1603 3400
Fragments arranged by size:
3400 1603 500 460
PciI A'CATG_T
Cuts at: 5115 5115
Size: 5963
PfoI T'CCnGG_A
Cuts at: 212 2015 2601 212
Size: 1803 586 3574
Fragments arranged by size:
3574 1803 586
PmeI GTTT'AAAC
Cuts at: 2962 2962
Size: 5963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 4409
Size: 32 5931
PpuMI rG'GwC_Cy
Cuts at: 258 324 258
Size: 66 5897
PshAI GACnn'nnGTC
Cuts at: 2011 2011
Size: 5963
PspOMI G'GGCC_C
Cuts at: 2169 2394 2169
Size: 225 5738
PspXI vC'TCGA_Gb
Cuts at: 1811 1811
Size: 5963
PstI C_TGCA'G
Cuts at: 347 452 1670 1703 1763 1943 3010 3695 347
Size: 105 1218 33 60 180 1067 685 2615
Fragments arranged by size:
2615 1218 1067 685 180 105 60 33
PvuI CG_AT'CG
Cuts at: 104 381 104
Size: 277 5686
PvuII CAG'CTG
Cuts at: 2705 3388 3748 2705
Size: 683 360 4920
Fragments arranged by size:
4920 683 360
RsrII CG'GwC_CG
Cuts at: 4158 4158
Size: 5963
SacI G_AGCT'C
Cuts at: 2977 2977
Size: 5963
SalI G'TCGA_C
Cuts at: 3000 3000
Size: 5963
SapI GCTCTTCn'nnn_
Cuts at: 611 3986 4196 611
Size: 3375 210 2378
Fragments arranged by size:
3375 2378 210
SbfI CC_TGCA'GG
Cuts at: 3010 3010
Size: 5963
ScaI AGT'ACT
Cuts at: 5480 5480
Size: 5963
SexAI A'CCwGG_T
Cuts at: 2954 2954
Size: 5963
SfcI C'TryA_G
Cuts at: 33 343 448 869 1666 1699 1759 1939 3006
Size: 310 105 421 797 33 60 180 1067
Cuts at: 3006 3691 4659 4850 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 1067 968 797 685 421 310 191
180 105 60 33
SfoI GGC'GCC
Cuts at: 1808 2132 3395 3643 1808
Size: 324 1263 248 4128
Fragments arranged by size:
4128 1263 324 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5963
SgrAI Cr'CCGG_yG
Cuts at: 1794 1794
Size: 5963
SmaI CCC'GGG
Cuts at: 142 2985 142
Size: 2843 3120
SmlI C'TyrA_G
Cuts at: 1811 3118 4033 4747 5009 1811
Size: 1307 915 714 262 2765
Fragments arranged by size:
2765 1307 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5963
SphI G_CATG'C
Cuts at: 2556 3016 3516 2556
Size: 460 500 5003
Fragments arranged by size:
5003 500 460
SspI AAT'ATT
Cuts at: 527 703 527
Size: 176 5787
StuI AGG'CCT
Cuts at: 2432 2432
Size: 5963
StyI C'CwwG_G
Cuts at: 107 3097 3451 107
Size: 2990 354 2619
Fragments arranged by size:
2990 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1353 1807 1852 2149 2415 2422 3546 3679 4138
Size: 454 45 297 266 7 1124 133 459
Cuts at: 4138 4347 1353
Size: 209 2969
Fragments arranged by size:
2969 1124 459 454 297 266 209 133
45 7
TatI w'GTAC_w
Cuts at: 1484 1985 2258 5478 1484
Size: 501 273 3220 1969
Fragments arranged by size:
3220 1969 501 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 872 904 5480 5512 872
Size: 32 4576 32 1323
Fragments arranged by size:
4576 1323 32 32
Tth111I GACn'n_nGTC
Cuts at: 3760 3760
Size: 5963
XbaI T'CTAG_A
Cuts at: 541 2994 541
Size: 2453 3510
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1431 3458 1431
Size: 2027 3936
XhoI C'TCGA_G
Cuts at: 1811 1811
Size: 5963
XmaI C'CCGG_G
Cuts at: 140 2983 140
Size: 2843 3120
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI BsiWI BsmBI
BspEI Bsu36I DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PflMI
PmlI PsiI PsrI SacII SanDI SfiI SnaBI SrfI
SwaI XmnI ZraI