Restriction map for
pF1KB3292
(Circular) (Six-base) MAPSORT of: pF1KB3292.seq Check: 8157 from: 1 to: 4790
pF1KB3292 4790 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1828 1828
Size: 4790
Acc65I G'GTAC_C
Cuts at: 1346 1806 1346
Size: 460 4330
AclI AA'CG_TT
Cuts at: 663 4630 663
Size: 3967 823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 2615 3047 3794 4062 174
Size: 2441 432 747 268 902
Fragments arranged by size:
2441 902 747 432 268
AflIII A'CryG_T
Cuts at: 1128 1137 1163 2142 3246 4223 1128
Size: 9 26 979 1104 977 1695
Fragments arranged by size:
1695 1104 979 977 26 9
AgeI A'CCGG_T
Cuts at: 3178 3178
Size: 4790
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 520 520
Size: 4790
AlwNI CAG_nnn'CTG
Cuts at: 862 1677 2095 3662 862
Size: 815 418 1567 1990
Fragments arranged by size:
1990 1567 815 418
ApaLI G'TGCA_C
Cuts at: 3560 3560
Size: 4790
ApoI r'AATT_y
Cuts at: 115 1073 1234 1242 1376 1472 1794 4143 4399
Size: 958 161 8 134 96 322 2349 256
Cuts at: 4399 115
Size: 506
Fragments arranged by size:
2349 958 506 322 256 161 134 96
8
AseI AT'TA_AT
Cuts at: 20 527 20
Size: 507 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4790
AvaI C'yCGr_G
Cuts at: 1810 1810
Size: 4790
BamHI G'GATC_C
Cuts at: 1815 1815
Size: 4790
BanI G'GyrC_C
Cuts at: 1346 1707 1806 2220 2468 2503 1346
Size: 361 99 414 248 35 3633
Fragments arranged by size:
3633 414 361 248 99 35
BanII G_rGCy'C
Cuts at: 1804 2834 1804
Size: 1030 3760
BbeI G_GCGC'C
Cuts at: 2224 2472 2224
Size: 248 4542
BciVI GTATCCnnnnn_n'
Cuts at: 449 920 2688 3449 4790 449
Size: 471 1768 761 1341 449
Fragments arranged by size:
1768 1341 761 471 449
BclI T'GATC_A
Cuts at: 674 719 827 2310 674
Size: 45 108 1483 3154
Fragments arranged by size:
3154 1483 108 45
BglII A'GATC_T
Cuts at: 394 2305 4342 394
Size: 1911 2037 842
Fragments arranged by size:
2037 1911 842
BlpI GC'TnA_GC
Cuts at: 1902 1902
Size: 4790
Bme1580I G_kGCm'C
Cuts at: 1712 2415 2508 3564 1712
Size: 703 93 1056 2938
Fragments arranged by size:
2938 1056 703 93
BmgBI CAC'GTC
Cuts at: 1384 1384
Size: 4790
BmrI ACTGGGnnnn_n'
Cuts at: 1573 1630 2002 2083 2418 4562 1573
Size: 57 372 81 335 2144 1801
Fragments arranged by size:
2144 1801 372 335 81 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3157 3157
Size: 4790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 302 784 1247 260
Size: 42 482 463 3803
Fragments arranged by size:
3803 482 463 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1966 2845 3337 3635 1966
Size: 879 492 298 3121
Fragments arranged by size:
3121 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4494 4494
Size: 4790
BsaAI yAC'GTr
Cuts at: 2143 2143
Size: 4790
BsaBI GATnn'nnATC
Cuts at: 6 2304 4341 6
Size: 2298 2037 455
Fragments arranged by size:
2298 2037 455
BsaHI Gr'CG_yC
Cuts at: 2221 2469 3171 4667 2221
Size: 248 702 1496 2344
Fragments arranged by size:
2344 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1979 2500 3178 3452 3599 4010 1979
Size: 521 678 274 147 411 2759
Fragments arranged by size:
2759 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 686 716 686
Size: 30 4760
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1073 1073
Size: 4790
BseYI C'CCAG_C
Cuts at: 1711 1742 2215 2973 3550 4046 1711
Size: 31 473 758 577 496 2455
Fragments arranged by size:
2455 758 577 496 473 31
BsiEI CG_ry'CG
Cuts at: 104 2332 2378 3586 104
Size: 2228 46 1208 1308
Fragments arranged by size:
2228 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 499 1804 2582 2772 3564 499
Size: 1305 778 190 792 1725
Fragments arranged by size:
1725 1305 792 778 190
BsmI GAATG_Cn'
Cuts at: 202 202
Size: 4790
Bsp1286I G_dGCh'C
Cuts at: 499 1712 1804 2415 2508 2582 2772 2834 3564
Size: 1213 92 611 93 74 190 62 730
Cuts at: 3564 499
Size: 1725
Fragments arranged by size:
1725 1213 730 611 190 93 92 74
62
BspEI T'CCGG_A
Cuts at: 4010 4010
Size: 4790
BspHI T'CATG_A
Cuts at: 270 3966 270
Size: 3696 1094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1840 2054 2356 2737 1840
Size: 214 302 381 3893
Fragments arranged by size:
3893 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3082 3136 4620 4785 50
Size: 3032 54 1484 165 55
Fragments arranged by size:
3032 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1734 2702 1734
Size: 968 3822
BsrFI r'CCGG_y
Cuts at: 2788 2969 3178 2788
Size: 181 209 4400
Fragments arranged by size:
4400 209 181
BsrGI T'GTAC_A
Cuts at: 748 748
Size: 4790
BssSI C'ACGA_G
Cuts at: 3061 3419 3061
Size: 358 4432
BstAPI GCAn_nnn'nTGC
Cuts at: 2167 2167
Size: 4790
BstBI TT'CG_AA
Cuts at: 651 1758 3151 651
Size: 1107 1393 2290
Fragments arranged by size:
2290 1393 1107
BstYI r'GATC_y
Cuts at: 1 394 1815 2282 2305 2640 2886 3898 3984
Size: 393 1421 467 23 335 246 1012 86
Cuts at: 3984 3996 4342 1
Size: 12 346 449
Fragments arranged by size:
1421 1012 467 449 393 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 977 1485 977
Size: 508 4282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2740 2921 3084 2740
Size: 181 163 4446
Fragments arranged by size:
4446 181 163
BtsI GCAGTG_nn'
Cuts at: 1099 1753 2033 1099
Size: 654 280 3856
Fragments arranged by size:
3856 654 280
ClaI AT'CG_AT
Cuts at: 821 821
Size: 4790
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 446 481 446
Size: 35 4755
DraI TTT'AAA
Cuts at: 345 1789 345
Size: 1444 3346
DrdI GACnn_nn'nnGTC
Cuts at: 2496 3354 2496
Size: 858 3932
EaeI y'GGCC_r
Cuts at: 776 2375 2549 2940 2967 4312 776
Size: 1599 174 391 27 1345 1254
Fragments arranged by size:
1599 1345 1254 391 174 27
EagI C'GGCC_G
Cuts at: 2375 2375
Size: 4790
EarI CTCTTCn'nnn_
Cuts at: 962 1688 2813 3023 962
Size: 726 1125 210 2729
Fragments arranged by size:
2729 1125 726 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2998 3308 3454 4600 2998
Size: 310 146 1146 3188
Fragments arranged by size:
3188 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1802 1802
Size: 4790
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 260 302 784 1247 2615 3047 3794 4062
Size: 86 42 482 463 1368 432 747 268
Cuts at: 4062 174
Size: 902
Fragments arranged by size:
1368 902 747 482 463 432 268 86
42
EcoNI CCTnn'n_nnAGG
Cuts at: 272 272
Size: 4790
EcoO109I rG'GnC_Cy
Cuts at: 277 1929 277
Size: 1652 3138
EcoRI G'AATT_C
Cuts at: 1234 1794 1234
Size: 560 4230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1263 2809 1263
Size: 1546 3244
FspI TGC'GCA
Cuts at: 2571 2571
Size: 4790
HaeII r_GCGC'y
Cuts at: 2224 2472 3494 4473 2224
Size: 248 1022 979 2541
Fragments arranged by size:
2541 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1282 1314 2632 2662 2664 2694 1282
Size: 32 1318 30 2 30 3378
Fragments arranged by size:
3378 1318 32 30 30 2
HincII GTy'rAC
Cuts at: 1829 1829
Size: 4790
KasI G'GCGC_C
Cuts at: 2220 2468 2220
Size: 248 4542
KpnI G_GTAC'C
Cuts at: 1350 1810 1350
Size: 460 4330
MluI A'CGCG_T
Cuts at: 4223 4223
Size: 4790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1113 1449 3461 3645 1113
Size: 336 2012 184 2258
Fragments arranged by size:
2258 2012 336 184
MscI TGG'CCA
Cuts at: 778 2551 778
Size: 1773 3017
MslI CAynn'nnrTG
Cuts at: 823 2779 2906 823
Size: 1956 127 2707
Fragments arranged by size:
2707 1956 127
MspA1I CmG'CkG
Cuts at: 1901 2215 2575 3588 3833 1901
Size: 314 360 1013 245 2858
Fragments arranged by size:
2858 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2971 2971
Size: 4790
NarI GG'CG_CC
Cuts at: 2221 2469 2221
Size: 248 4542
NcoI C'CATG_G
Cuts at: 977 1485 977
Size: 508 4282
NdeI CA'TA_TG
Cuts at: 1032 1032
Size: 4790
NgoMIV G'CCGG_C
Cuts at: 2969 2969
Size: 4790
NspI r_CATG'y
Cuts at: 1124 1132 1141 1660 1843 2343 3250 1124
Size: 8 9 519 183 500 907 2664
Fragments arranged by size:
2664 907 519 500 183 9 8
PciI A'CATG_T
Cuts at: 1128 1137 3246 1128
Size: 9 2109 2672
Fragments arranged by size:
2672 2109 9
PflMI CCAn_nnn'nTGG
Cuts at: 1491 1491
Size: 4790
PfoI T'CCnGG_A
Cuts at: 279 728 1170 279
Size: 449 442 3899
Fragments arranged by size:
3899 449 442
PmeI GTTT'AAAC
Cuts at: 1789 1789
Size: 4790
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 3929
Size: 32 4758
PpuMI rG'GwC_Cy
Cuts at: 277 277
Size: 4790
PstI C_TGCA'G
Cuts at: 1655 1837 2522 1655
Size: 182 685 3923
Fragments arranged by size:
3923 685 182
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4790
PvuII CAG'CTG
Cuts at: 2215 2575 2215
Size: 360 4430
RsrII CG'GwC_CG
Cuts at: 2985 2985
Size: 4790
SacI G_AGCT'C
Cuts at: 1804 1804
Size: 4790
SalI G'TCGA_C
Cuts at: 1827 1827
Size: 4790
SapI GCTCTTCn'nnn_
Cuts at: 2813 3023 2813
Size: 210 4580
SbfI CC_TGCA'GG
Cuts at: 1837 1837
Size: 4790
ScaI AGT'ACT
Cuts at: 4307 4307
Size: 4790
SfcI C'TryA_G
Cuts at: 33 1651 1833 2518 3511 3702 33
Size: 1618 182 685 993 191 1121
Fragments arranged by size:
1618 1121 993 685 191 182
SfoI GGC'GCC
Cuts at: 2222 2470 2222
Size: 248 4542
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4790
SmaI CCC'GGG
Cuts at: 1812 1812
Size: 4790
SmlI C'TyrA_G
Cuts at: 1945 2860 3352 3614 1945
Size: 915 492 262 3121
Fragments arranged by size:
3121 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4790
SphI G_CATG'C
Cuts at: 1843 2343 1843
Size: 500 4290
SspI AAT'ATT
Cuts at: 1781 1781
Size: 4790
StuI AGG'CCT
Cuts at: 603 989 603
Size: 386 4404
StyI C'CwwG_G
Cuts at: 977 1485 1924 2278 977
Size: 508 439 354 3489
Fragments arranged by size:
3489 508 439 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1727 2373 2506 2965 3174 1727
Size: 646 133 459 209 3343
Fragments arranged by size:
3343 646 459 209 133
TatI w'GTAC_w
Cuts at: 258 748 848 4305 258
Size: 490 100 3457 743
Fragments arranged by size:
3457 743 490 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 686 718 4307 4339 686
Size: 32 3589 32 1137
Fragments arranged by size:
3589 1137 32 32
Tth111I GACn'n_nGTC
Cuts at: 2587 2587
Size: 4790
XbaI T'CTAG_A
Cuts at: 249 1434 1821 249
Size: 1185 387 3218
Fragments arranged by size:
3218 1185 387
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2285 2285
Size: 4790
XmaI C'CCGG_G
Cuts at: 1810 1810
Size: 4790
XmnI GAAnn'nnTTC
Cuts at: 560 1246 560
Size: 686 4104
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI