Restriction map for
pF1KB3289
(Circular) (Six-base) MAPSORT of: pF1KB3289.seq Check: 863 from: 1 to: 5522
pF1KB3289 5522 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 2560 2560
Size: 5522
Acc65I G'GTAC_C
Cuts at: 2538 2538
Size: 5522
AclI AA'CG_TT
Cuts at: 5362 5362
Size: 5522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 873 1206 1225 1290 1477 1626 1710 1813
Size: 316 333 19 65 187 149 84 103
Cuts at: 1813 1962 2214 2298 2382 3347 3779 4132 4794
Size: 149 252 84 84 965 432 353 662
Cuts at: 4794 557
Size: 1285
Fragments arranged by size:
1285 965 662 432 353 333 316 252
187 149 149 103 84 84 84 65
19
AflII C'TTAA_G
Cuts at: 1027 1027
Size: 5522
AflIII A'CryG_T
Cuts at: 949 1011 2874 4674 4955 949
Size: 62 1863 1800 281 1516
Fragments arranged by size:
1863 1800 1516 281 62
AleI CACnn'nnGTG
Cuts at: 764 764
Size: 5522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 443 443
Size: 5522
AlwNI CAG_nnn'CTG
Cuts at: 2827 4265 2827
Size: 1438 4084
ApaI G_GGCC'C
Cuts at: 187 187
Size: 5522
ApaLI G'TGCA_C
Cuts at: 2072 2182 4360 2072
Size: 110 2178 3234
Fragments arranged by size:
3234 2178 110
ApoI r'AATT_y
Cuts at: 336 530 674 1051 1314 1650 1916 2526 4875
Size: 194 144 377 263 336 266 610 2349
Cuts at: 4875 5131 336
Size: 256 727
Fragments arranged by size:
2349 727 610 377 336 266 263 256
194 144
AseI AT'TA_AT
Cuts at: 20 20
Size: 5522
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
AvaI C'yCGr_G
Cuts at: 2542 2542
Size: 5522
BamHI G'GATC_C
Cuts at: 1928 2547 1928
Size: 619 4903
BanI G'GyrC_C
Cuts at: 2538 2952 3200 3235 2538
Size: 414 248 35 4825
Fragments arranged by size:
4825 414 248 35
BanII G_rGCy'C
Cuts at: 187 1822 2536 3566 187
Size: 1635 714 1030 2143
Fragments arranged by size:
2143 1635 1030 714
BbeI G_GCGC'C
Cuts at: 2956 3204 2956
Size: 248 5274
BbsI GAAGACnn'nnnn_
Cuts at: 333 1483 1968 2239 2304 2315 333
Size: 1150 485 271 65 11 3540
Fragments arranged by size:
3540 1150 485 271 65 11
BciVI GTATCCnnnnn_n'
Cuts at: 3420 4476 5522 3420
Size: 1056 1046 3420
Fragments arranged by size:
3420 1056 1046
BclI T'GATC_A
Cuts at: 461 825 3042 461
Size: 364 2217 2941
Fragments arranged by size:
2941 2217 364
BfrBI ATG'CAT
Cuts at: 1291 2172 1291
Size: 881 4641
BglII A'GATC_T
Cuts at: 1908 3037 5074 1908
Size: 1129 2037 2356
Fragments arranged by size:
2356 2037 1129
BlpI GC'TnA_GC
Cuts at: 2634 2634
Size: 5522
Bme1580I G_kGCm'C
Cuts at: 187 2076 2186 3147 3240 4364 187
Size: 1889 110 961 93 1124 1345
Fragments arranged by size:
1889 1345 1124 961 110 93
BmgBI CAC'GTC
Cuts at: 144 144
Size: 5522
BmrI ACTGGGnnnn_n'
Cuts at: 593 979 1274 2366 2734 2815 3150 5294 593
Size: 386 295 1092 368 81 335 2144 821
Fragments arranged by size:
2144 1092 821 386 368 335 295 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3889 3889
Size: 5522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 885 1538 1622 2126 885
Size: 653 84 504 4281
Fragments arranged by size:
4281 653 504 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 2226 2698 3577 4291 4589 627
Size: 1599 472 879 714 298 1560
Fragments arranged by size:
1599 1560 879 714 472 298
BsaI GGTCTCn'nnnn_
Cuts at: 5226 5226
Size: 5522
BsaAI yAC'GTr
Cuts at: 1095 2875 1095
Size: 1780 3742
BsaBI GATnn'nnATC
Cuts at: 6 914 3036 5073 6
Size: 908 2122 2037 455
Fragments arranged by size:
2122 2037 908 455
BsaHI Gr'CG_yC
Cuts at: 2953 3201 3903 5399 2953
Size: 248 702 1496 3076
Fragments arranged by size:
3076 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2711 3232 3910 4321 4468 4742 2711
Size: 521 678 411 147 274 3491
Fragments arranged by size:
3491 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 1219 1387 1807 1975 178
Size: 1041 168 420 168 3725
Fragments arranged by size:
3725 1041 420 168 168
BseYI C'CCAG_C
Cuts at: 180 2947 3705 4370 4778 180
Size: 2767 758 665 408 924
Fragments arranged by size:
2767 924 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2067 3064 3110 4340 104
Size: 1963 997 46 1230 1286
Fragments arranged by size:
1963 1286 1230 997 46
BsiHKAI G_wGCw'C
Cuts at: 1822 2076 2186 2536 3314 3504 4364 1822
Size: 254 110 350 778 190 860 2980
Fragments arranged by size:
2980 860 778 350 254 190 110
Bsp1286I G_dGCh'C
Cuts at: 187 1822 2076 2186 2536 3147 3240 3314 3504
Size: 1635 254 110 350 611 93 74 190
Cuts at: 3504 3566 4364 187
Size: 62 798 1345
Fragments arranged by size:
1635 1345 798 611 350 254 190 110
93 74 62
BspHI T'CATG_A
Cuts at: 741 3954 741
Size: 3213 2309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 245 2572 2786 3088 3469 245
Size: 2327 214 302 381 2298
Fragments arranged by size:
2327 2298 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3814 3868 5352 5517 50
Size: 3764 54 1484 165 55
Fragments arranged by size:
3764 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1129 1164 1629 3434 1129
Size: 35 465 1805 3217
Fragments arranged by size:
3217 1805 465 35
BsrFI r'CCGG_y
Cuts at: 3520 3701 3520
Size: 181 5341
BssSI C'ACGA_G
Cuts at: 3793 4501 3793
Size: 708 4814
BstAPI GCAn_nnn'nTGC
Cuts at: 2899 2899
Size: 5522
BstBI TT'CG_AA
Cuts at: 3883 3883
Size: 5522
BstYI r'GATC_y
Cuts at: 1 1908 1928 2547 3014 3037 3372 3618 3924
Size: 1907 20 619 467 23 335 246 306
Cuts at: 3924 3936 4022 5074 1
Size: 12 86 1052 449
Fragments arranged by size:
1907 1052 619 467 449 335 306 246
86 23 20 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3472 3653 3816 3472
Size: 181 163 5178
Fragments arranged by size:
5178 181 163
BtsI GCAGTG_nn'
Cuts at: 134 2765 134
Size: 2631 2891
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1611 1646 1611
Size: 35 5487
DraI TTT'AAA
Cuts at: 2521 2521
Size: 5522
DrdI GACnn_nn'nnGTC
Cuts at: 519 3228 4572 519
Size: 2709 1344 1469
Fragments arranged by size:
2709 1469 1344
EaeI y'GGCC_r
Cuts at: 3107 3281 3672 3699 5044 3107
Size: 174 391 27 1345 3585
Fragments arranged by size:
3585 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3107 3107
Size: 5522
EarI CTCTTCn'nnn_
Cuts at: 389 1630 1871 2050 3545 3755 389
Size: 1241 241 179 1495 210 2156
Fragments arranged by size:
2156 1495 1241 241 210 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3730 4472 4618 5332 3730
Size: 742 146 714 3920
Fragments arranged by size:
3920 742 714 146
EcoICRI GAG'CTC
Cuts at: 1820 2534 1820
Size: 714 4808
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 873 885 1206 1225 1290 1477 1538 1622
Size: 316 12 321 19 65 187 61 84
Cuts at: 1622 1626 1710 1813 1962 2126 2214 2298 2382
Size: 4 84 103 149 164 88 84 84
Cuts at: 2382 3347 3779 4132 4794 557
Size: 965 432 353 662 1285
Fragments arranged by size:
1285 965 662 432 353 321 316 187
164 149 103 88 84 84 84 84
65 61 19 12 4
EcoO109I rG'GnC_Cy
Cuts at: 184 1217 2661 184
Size: 1033 1444 3045
Fragments arranged by size:
3045 1444 1033
EcoRI G'AATT_C
Cuts at: 1051 1314 1650 2526 1051
Size: 263 336 876 4047
Fragments arranged by size:
4047 876 336 263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 415 2172 3541 415
Size: 1757 1369 2396
Fragments arranged by size:
2396 1757 1369
FspI TGC'GCA
Cuts at: 3303 3303
Size: 5522
HaeII r_GCGC'y
Cuts at: 1067 2956 3204 4434 5205 1067
Size: 1889 248 1230 771 1384
Fragments arranged by size:
1889 1384 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 370 402 723 755 810 842 1293 1325 1557
Size: 32 321 32 55 32 451 32 232
Cuts at: 1557 1589 2049 2061 2081 2093 3364 3394 3396
Size: 32 460 12 20 12 1271 30 2
Cuts at: 3396 3426 370
Size: 30 2466
Fragments arranged by size:
2466 1271 460 451 321 232 55 32
32 32 32 32 30 30 20 12
12 2
HincII GTy'rAC
Cuts at: 2561 2561
Size: 5522
KasI G'GCGC_C
Cuts at: 2952 3200 2952
Size: 248 5274
KpnI G_GTAC'C
Cuts at: 2542 2542
Size: 5522
MluI A'CGCG_T
Cuts at: 4955 4955
Size: 5522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 709 1291 2047 4281 4465 709
Size: 582 756 2234 184 1766
Fragments arranged by size:
2234 1766 756 582 184
MscI TGG'CCA
Cuts at: 3283 3283
Size: 5522
MslI CAynn'nnrTG
Cuts at: 112 743 764 1113 1281 1365 1533 1617 1853
Size: 631 21 349 168 84 168 84 236
Cuts at: 1853 1869 2037 2121 2205 2261 2289 2441 3511
Size: 16 168 84 84 56 28 152 1070
Cuts at: 3511 3638 112
Size: 127 1996
Fragments arranged by size:
1996 1070 631 349 236 168 168 168
152 127 84 84 84 84 56 28
21 16
MspA1I CmG'CkG
Cuts at: 1842 2633 2947 3307 4091 4336 1842
Size: 791 314 360 784 245 3028
Fragments arranged by size:
3028 791 784 360 314 245
NaeI GCC'GGC
Cuts at: 3703 3703
Size: 5522
NarI GG'CG_CC
Cuts at: 2953 3201 2953
Size: 248 5274
NdeI CA'TA_TG
Cuts at: 1534 1534
Size: 5522
NgoMIV G'CCGG_C
Cuts at: 3701 3701
Size: 5522
NsiI A_TGCA'T
Cuts at: 1293 2174 1293
Size: 881 4641
NspI r_CATG'y
Cuts at: 953 1015 1295 2172 2575 3075 4678 953
Size: 62 280 877 403 500 1603 1797
Fragments arranged by size:
1797 1603 877 500 403 280 62
PciI A'CATG_T
Cuts at: 949 1011 4674 949
Size: 62 3663 1797
Fragments arranged by size:
3663 1797 62
PflMI CCAn_nnn'nTGG
Cuts at: 728 728
Size: 5522
PmeI GTTT'AAAC
Cuts at: 2521 2521
Size: 5522
PmlI CAC'GTG
Cuts at: 1095 1095
Size: 5522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 5490
PsiI TTA'TAA
Cuts at: 1196 2372 1196
Size: 1176 4346
PspOMI G'GGCC_C
Cuts at: 183 183
Size: 5522
PstI C_TGCA'G
Cuts at: 2569 3254 2569
Size: 685 4837
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5522
PvuII CAG'CTG
Cuts at: 1842 2947 3307 1842
Size: 1105 360 4057
Fragments arranged by size:
4057 1105 360
RsrII CG'GwC_CG
Cuts at: 3717 3717
Size: 5522
SacI G_AGCT'C
Cuts at: 1822 2536 1822
Size: 714 4808
SalI G'TCGA_C
Cuts at: 2559 2559
Size: 5522
SapI GCTCTTCn'nnn_
Cuts at: 389 1630 3545 3755 389
Size: 1241 1915 210 2156
Fragments arranged by size:
2156 1915 1241 210
SbfI CC_TGCA'GG
Cuts at: 2569 2569
Size: 5522
ScaI AGT'ACT
Cuts at: 2227 5039 2227
Size: 2812 2710
SexAI A'CCwGG_T
Cuts at: 442 503 442
Size: 61 5461
SfcI C'TryA_G
Cuts at: 33 281 1072 1923 2332 2565 3250 4218 4409
Size: 248 791 851 409 233 685 968 191
Cuts at: 4409 33
Size: 1146
Fragments arranged by size:
1146 968 851 791 685 409 248 233
191
SfoI GGC'GCC
Cuts at: 2954 3202 2954
Size: 248 5274
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
SmaI CCC'GGG
Cuts at: 2544 2544
Size: 5522
SmlI C'TyrA_G
Cuts at: 606 1027 2241 2677 3592 4306 4568 606
Size: 421 1214 436 915 714 262 1560
Fragments arranged by size:
1560 1214 915 714 436 421 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5522
SphI G_CATG'C
Cuts at: 2575 3075 2575
Size: 500 5022
StuI AGG'CCT
Cuts at: 1231 1987 2239 1231
Size: 756 252 4514
Fragments arranged by size:
4514 756 252
StyI C'CwwG_G
Cuts at: 2656 3010 2656
Size: 354 5168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2053 2309 3105 3238 3697 3906 2053
Size: 256 796 133 459 209 3669
Fragments arranged by size:
3669 796 459 256 209 133
TatI w'GTAC_w
Cuts at: 756 1008 1537 2225 5037 756
Size: 252 529 688 2812 1241
Fragments arranged by size:
2812 1241 688 529 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5039 5071 5039
Size: 32 5490
Tth111I GACn'n_nGTC
Cuts at: 645 3319 645
Size: 2674 2848
XbaI T'CTAG_A
Cuts at: 2553 2553
Size: 5522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3017 3017
Size: 5522
XmaI C'CCGG_G
Cuts at: 2542 2542
Size: 5522
XmnI GAAnn'nnTTC
Cuts at: 115 634 1135 1276 1639 1723 1975 2059 2143
Size: 519 501 141 363 84 252 84 84
Cuts at: 2143 115
Size: 3494
Fragments arranged by size:
3494 519 501 363 252 141 84 84
84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PflMI PmeI PmlI PpiI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI AscI AvrII
BaeI BbvCI BcgI BglI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
PacI PasI PfoI PpuMI PshAI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI