Restriction map for
pF1KB3282
(Circular) (Six-base) MAPSORT of: pF1KB3282.seq Check: 2877 from: 1 to: 5333
pF1KB3282 5333 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 647 2371 647
Size: 1724 3609
Acc65I G'GTAC_C
Cuts at: 602 2349 602
Size: 1747 3586
AclI AA'CG_TT
Cuts at: 1116 5173 1116
Size: 4057 1276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 1048 1653 3158 3590 3943 4605 615
Size: 433 605 1505 432 353 662 1343
Fragments arranged by size:
1505 1343 662 605 433 432 353
AflIII A'CryG_T
Cuts at: 2685 4485 4766 2685
Size: 1800 281 3252
Fragments arranged by size:
3252 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 133 1146 2638 4076 133
Size: 1013 1492 1438 1390
Fragments arranged by size:
1492 1438 1390 1013
ApaI G_GGCC'C
Cuts at: 875 875
Size: 5333
ApaLI G'TGCA_C
Cuts at: 991 4171 991
Size: 3180 2153
ApoI r'AATT_y
Cuts at: 386 937 1287 1673 2072 2234 2273 2337 4686
Size: 551 350 386 399 162 39 64 2349
Cuts at: 4686 4942 386
Size: 256 777
Fragments arranged by size:
2349 777 551 399 386 350 256 162
64 39
AseI AT'TA_AT
Cuts at: 20 547 20
Size: 527 4806
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
AvaI C'yCGr_G
Cuts at: 996 1370 2353 996
Size: 374 983 3976
Fragments arranged by size:
3976 983 374
BamHI G'GATC_C
Cuts at: 2358 2358
Size: 5333
BanI G'GyrC_C
Cuts at: 602 2349 2763 3011 3046 602
Size: 1747 414 248 35 2889
Fragments arranged by size:
2889 1747 414 248 35
BanII G_rGCy'C
Cuts at: 875 1688 2347 3377 875
Size: 813 659 1030 2831
Fragments arranged by size:
2831 1030 813 659
BbeI G_GCGC'C
Cuts at: 2767 3015 2767
Size: 248 5085
BbsI GAAGACnn'nnnn_
Cuts at: 1322 1768 2291 1322
Size: 446 523 4364
Fragments arranged by size:
4364 523 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2016 2050 2016
Size: 34 5299
BciVI GTATCCnnnnn_n'
Cuts at: 1864 2166 3231 4287 5333 1864
Size: 302 1065 1056 1046 1864
Fragments arranged by size:
1864 1065 1056 1046 302
BclI T'GATC_A
Cuts at: 695 2853 695
Size: 2158 3175
BfrBI ATG'CAT
Cuts at: 1623 1818 1623
Size: 195 5138
BglII A'GATC_T
Cuts at: 1842 2848 4885 1842
Size: 1006 2037 2290
Fragments arranged by size:
2290 2037 1006
BlpI GC'TnA_GC
Cuts at: 2445 2445
Size: 5333
Bme1580I G_kGCm'C
Cuts at: 875 995 2958 3051 4175 875
Size: 120 1963 93 1124 2033
Fragments arranged by size:
2033 1963 1124 120 93
BmrI ACTGGGnnnn_n'
Cuts at: 2545 2626 2961 5105 2545
Size: 81 335 2144 2773
Fragments arranged by size:
2773 2144 335 81
BmtI G_CTAG'C
Cuts at: 2230 2230
Size: 5333
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3700 3700
Size: 5333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 1322 362
Size: 960 4373
Bpu10I CC'TnA_GC
Cuts at: 1472 1472
Size: 5333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 2509 3388 4102 4400 438
Size: 2071 879 714 298 1371
Fragments arranged by size:
2071 1371 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 310 5037 310
Size: 4727 606
BsaAI yAC'GTr
Cuts at: 2686 2686
Size: 5333
BsaBI GATnn'nnATC
Cuts at: 6 568 1529 2847 4884 6
Size: 562 961 1318 2037 455
Fragments arranged by size:
2037 1318 961 562 455
BsaHI Gr'CG_yC
Cuts at: 2764 3012 3714 5210 2764
Size: 248 702 1496 2887
Fragments arranged by size:
2887 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2522 3043 3721 4132 4279 4553 2522
Size: 521 678 411 147 274 3302
Fragments arranged by size:
3302 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1155 1185 1460 1490 1568 1598 1155
Size: 30 275 30 78 30 4890
Fragments arranged by size:
4890 275 78 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 869 1715 869
Size: 846 4487
BseYI C'CCAG_C
Cuts at: 2758 3516 4181 4589 2758
Size: 758 665 408 3502
Fragments arranged by size:
3502 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 5333
BsiEI CG_ry'CG
Cuts at: 104 2875 2921 4151 104
Size: 2771 46 1230 1286
Fragments arranged by size:
2771 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 995 2347 3125 3315 4175 995
Size: 1352 778 190 860 2153
Fragments arranged by size:
2153 1352 860 778 190
BsmI GAATG_Cn'
Cuts at: 354 616 1625 354
Size: 262 1009 4062
Fragments arranged by size:
4062 1009 262
Bsp1286I G_dGCh'C
Cuts at: 875 995 1688 2347 2958 3051 3125 3315 3377
Size: 120 693 659 611 93 74 190 62
Cuts at: 3377 4175 875
Size: 798 2033
Fragments arranged by size:
2033 798 693 659 611 190 120 93
74 62
BspHI T'CATG_A
Cuts at: 1130 3765 1130
Size: 2635 2698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2383 2597 2899 3280 2383
Size: 214 302 381 4436
Fragments arranged by size:
4436 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3625 3679 5163 5328 50
Size: 3575 54 1484 165 55
Fragments arranged by size:
3575 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 306 3245 306
Size: 2939 2394
BsrFI r'CCGG_y
Cuts at: 3331 3512 3331
Size: 181 5152
BsrGI T'GTAC_A
Cuts at: 1376 1376
Size: 5333
BssSI C'ACGA_G
Cuts at: 3604 4312 3604
Size: 708 4625
BstAPI GCAn_nnn'nTGC
Cuts at: 2710 2710
Size: 5333
BstBI TT'CG_AA
Cuts at: 3694 3694
Size: 5333
BstYI r'GATC_y
Cuts at: 1 1842 2358 2825 2848 3183 3429 3735 3747
Size: 1841 516 467 23 335 246 306 12
Cuts at: 3747 3833 4885 1
Size: 86 1052 449
Fragments arranged by size:
1841 1052 516 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 252 252
Size: 5333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3283 3464 3627 3283
Size: 181 163 4989
Fragments arranged by size:
4989 181 163
BtsI GCAGTG_nn'
Cuts at: 905 1176 1202 1277 1364 2576 905
Size: 271 26 75 87 1212 3662
Fragments arranged by size:
3662 1212 271 87 75 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1632 1667 1632
Size: 35 5298
DraI TTT'AAA
Cuts at: 239 2332 239
Size: 2093 3240
DraIII CAC_nnn'GTG
Cuts at: 441 441
Size: 5333
DrdI GACnn_nn'nnGTC
Cuts at: 3039 4383 3039
Size: 1344 3989
EaeI y'GGCC_r
Cuts at: 249 1233 2918 3092 3483 3510 4855 249
Size: 984 1685 174 391 27 1345 727
Fragments arranged by size:
1685 1345 984 727 391 174 27
EagI C'GGCC_G
Cuts at: 2918 2918
Size: 5333
EarI CTCTTCn'nnn_
Cuts at: 1672 3356 3566 1672
Size: 1684 210 3439
Fragments arranged by size:
3439 1684 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3541 4283 4429 5143 3541
Size: 742 146 714 3731
Fragments arranged by size:
3731 742 714 146
EcoICRI GAG'CTC
Cuts at: 2345 2345
Size: 5333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 615 1048 1322 1653 3158 3590 3943 4605
Size: 253 433 274 331 1505 432 353 662
Cuts at: 4605 362
Size: 1090
Fragments arranged by size:
1505 1090 662 433 432 353 331 274
253
EcoO109I rG'GnC_Cy
Cuts at: 2472 2472
Size: 5333
EcoRI G'AATT_C
Cuts at: 1673 2234 2337 1673
Size: 561 103 4669
Fragments arranged by size:
4669 561 103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3352 3352
Size: 5333
FspI TGC'GCA
Cuts at: 3114 3114
Size: 5333
HaeII r_GCGC'y
Cuts at: 2160 2767 3015 4245 5016 2160
Size: 607 248 1230 771 2477
Fragments arranged by size:
2477 1230 771 607 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1623 1655 1918 1950 2308 2340 3175 3205 3207
Size: 32 263 32 358 32 835 30 2
Cuts at: 3207 3237 1623
Size: 30 3719
Fragments arranged by size:
3719 835 358 263 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 588 2372 588
Size: 1784 3549
HindIII A'AGCT_T
Cuts at: 1079 1079
Size: 5333
KasI G'GCGC_C
Cuts at: 2763 3011 2763
Size: 248 5085
KpnI G_GTAC'C
Cuts at: 606 2353 606
Size: 1747 3586
MfeI C'AATT_G
Cuts at: 866 2167 866
Size: 1301 4032
MluI A'CGCG_T
Cuts at: 4766 4766
Size: 5333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4092 4276 4092
Size: 184 5149
MscI TGG'CCA
Cuts at: 251 1235 3094 251
Size: 984 1859 2490
Fragments arranged by size:
2490 1859 984
MslI CAynn'nnrTG
Cuts at: 656 3322 3449 656
Size: 2666 127 2540
Fragments arranged by size:
2666 2540 127
MspA1I CmG'CkG
Cuts at: 133 2444 2758 3118 3902 4147 133
Size: 2311 314 360 784 245 1319
Fragments arranged by size:
2311 1319 784 360 314 245
NaeI GCC'GGC
Cuts at: 3514 3514
Size: 5333
NarI GG'CG_CC
Cuts at: 2764 3012 2764
Size: 248 5085
NdeI CA'TA_TG
Cuts at: 116 198 1733 116
Size: 82 1535 3716
Fragments arranged by size:
3716 1535 82
NgoMIV G'CCGG_C
Cuts at: 3512 3512
Size: 5333
NheI G'CTAG_C
Cuts at: 2226 2226
Size: 5333
NsiI A_TGCA'T
Cuts at: 1625 1820 1625
Size: 195 5138
NspI r_CATG'y
Cuts at: 2386 2886 4489 2386
Size: 500 1603 3230
Fragments arranged by size:
3230 1603 500
PciI A'CATG_T
Cuts at: 4485 4485
Size: 5333
PflMI CCAn_nnn'nTGG
Cuts at: 768 1845 768
Size: 1077 4256
PmeI GTTT'AAAC
Cuts at: 2332 2332
Size: 5333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 3779
Size: 32 5301
PsiI TTA'TAA
Cuts at: 689 1421 689
Size: 732 4601
PspOMI G'GGCC_C
Cuts at: 871 871
Size: 5333
PspXI vC'TCGA_Gb
Cuts at: 1370 1370
Size: 5333
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1841 1873 1841
Size: 32 5301
PstI C_TGCA'G
Cuts at: 288 2380 3065 288
Size: 2092 685 2556
Fragments arranged by size:
2556 2092 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5333
PvuII CAG'CTG
Cuts at: 133 2758 3118 133
Size: 2625 360 2348
Fragments arranged by size:
2625 2348 360
RsrII CG'GwC_CG
Cuts at: 3528 3528
Size: 5333
SacI G_AGCT'C
Cuts at: 2347 2347
Size: 5333
SalI G'TCGA_C
Cuts at: 2370 2370
Size: 5333
SapI GCTCTTCn'nnn_
Cuts at: 3356 3566 3356
Size: 210 5123
SbfI CC_TGCA'GG
Cuts at: 2380 2380
Size: 5333
ScaI AGT'ACT
Cuts at: 4850 4850
Size: 5333
SfcI C'TryA_G
Cuts at: 33 284 2376 3061 4029 4220 33
Size: 251 2092 685 968 191 1146
Fragments arranged by size:
2092 1146 968 685 251 191
SfoI GGC'GCC
Cuts at: 2765 3013 2765
Size: 248 5085
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
SmaI CCC'GGG
Cuts at: 2355 2355
Size: 5333
SmlI C'TyrA_G
Cuts at: 453 996 1370 2488 3403 4117 4379 453
Size: 543 374 1118 915 714 262 1407
Fragments arranged by size:
1407 1118 915 714 543 374 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5333
SphI G_CATG'C
Cuts at: 2386 2886 2386
Size: 500 4833
StyI C'CwwG_G
Cuts at: 2467 2821 2467
Size: 354 4979
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2916 3049 3508 3717 2916
Size: 133 459 209 4532
Fragments arranged by size:
4532 459 209 133
TatI w'GTAC_w
Cuts at: 1376 4848 1376
Size: 3472 1861
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 485 517 766 798 881 913 1155 1187 1568
Size: 32 249 32 83 32 242 32 381
Cuts at: 1568 1600 4850 4882 485
Size: 32 3250 32 936
Fragments arranged by size:
3250 936 381 249 242 83 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3130 3130
Size: 5333
XbaI T'CTAG_A
Cuts at: 2364 2364
Size: 5333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1462 2828 1462
Size: 1366 3967
XhoI C'TCGA_G
Cuts at: 996 1370 996
Size: 374 4959
XmaI C'CCGG_G
Cuts at: 2353 2353
Size: 5333
XmnI GAAnn'nnTTC
Cuts at: 1553 1553
Size: 5333
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NsiI
NspI PciI PflMI PmeI PpiI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BglI BmgBI BsiWI
BsmBI BspEI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
EcoNI EcoRV FseI FspAI HpaI NcoI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
ZraI