Restriction map for
pF1KB3281
(Circular) (Six-base) MAPSORT of: pF1KB3281.seq Check: 5981 from: 1 to: 5474
pF1KB3281 5474 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 720 720
Size: 5474
AccI GT'mk_AC
Cuts at: 2141 2512 2141
Size: 371 5103
Acc65I G'GTAC_C
Cuts at: 2490 2490
Size: 5474
AclI AA'CG_TT
Cuts at: 5314 5314
Size: 5474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1153 1338 3299 3731 4478 4746 376
Size: 777 185 1961 432 747 268 1104
Fragments arranged by size:
1961 1104 777 747 432 268 185
AflIII A'CryG_T
Cuts at: 395 2111 2330 2826 3930 4907 395
Size: 1716 219 496 1104 977 962
Fragments arranged by size:
1716 1104 977 962 496 219
AgeI A'CCGG_T
Cuts at: 3862 3862
Size: 5474
AleI CACnn'nnGTG
Cuts at: 1626 1626
Size: 5474
AlwNI CAG_nnn'CTG
Cuts at: 288 309 771 825 888 1014 1131 1159 1579
Size: 21 462 54 63 126 117 28 420
Cuts at: 1579 1716 2779 4346 288
Size: 137 1063 1567 1416
Fragments arranged by size:
1567 1416 1063 462 420 137 126 117
63 54 28 21
ApaI G_GGCC'C
Cuts at: 908 908
Size: 5474
ApaLI G'TGCA_C
Cuts at: 1192 4244 1192
Size: 3052 2422
ApoI r'AATT_y
Cuts at: 1423 1648 1791 2478 4827 5083 1423
Size: 225 143 687 2349 256 1814
Fragments arranged by size:
2349 1814 687 256 225 143
AseI AT'TA_AT
Cuts at: 20 20
Size: 5474
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
AvaI C'yCGr_G
Cuts at: 910 2494 910
Size: 1584 3890
BamHI G'GATC_C
Cuts at: 650 2450 2499 650
Size: 1800 49 3625
Fragments arranged by size:
3625 1800 49
BanI G'GyrC_C
Cuts at: 1336 1587 2490 2904 3152 3187 1336
Size: 251 903 414 248 35 3623
Fragments arranged by size:
3623 903 414 251 248 35
BanII G_rGCy'C
Cuts at: 688 908 2423 2488 3518 688
Size: 220 1515 65 1030 2644
Fragments arranged by size:
2644 1515 1030 220 65
BbeI G_GCGC'C
Cuts at: 2908 3156 2908
Size: 248 5226
BbsI GAAGACnn'nnnn_
Cuts at: 817 1141 817
Size: 324 5150
BciVI GTATCCnnnnn_n'
Cuts at: 113 2194 3372 4133 5474 113
Size: 2081 1178 761 1341 113
Fragments arranged by size:
2081 1341 1178 761 113
BclI T'GATC_A
Cuts at: 2994 2994
Size: 5474
BglII A'GATC_T
Cuts at: 246 1292 2989 5026 246
Size: 1046 1697 2037 694
Fragments arranged by size:
2037 1697 1046 694
BlpI GC'TnA_GC
Cuts at: 128 682 995 2586 128
Size: 554 313 1591 3016
Fragments arranged by size:
3016 1591 554 313
Bme1580I G_kGCm'C
Cuts at: 374 908 1196 1350 3099 3192 4248 374
Size: 534 288 154 1749 93 1056 1600
Fragments arranged by size:
1749 1600 1056 534 288 154 93
BmgBI CAC'GTC
Cuts at: 927 927
Size: 5474
BmrI ACTGGGnnnn_n'
Cuts at: 207 1024 1440 1770 2686 2767 3102 5246 207
Size: 817 416 330 916 81 335 2144 435
Fragments arranged by size:
2144 916 817 435 416 335 330 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3841 3841
Size: 5474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 373 476 517 703 760 1762 2404 310
Size: 63 103 41 186 57 1002 642 3380
Fragments arranged by size:
3380 1002 642 186 103 63 57 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 1846 2650 3529 4021 4319 1240
Size: 606 804 879 492 298 2395
Fragments arranged by size:
2395 879 804 606 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5178 5178
Size: 5474
BsaAI yAC'GTr
Cuts at: 398 2112 2205 2279 2827 398
Size: 1714 93 74 548 3045
Fragments arranged by size:
3045 1714 548 93 74
BsaBI GATnn'nnATC
Cuts at: 6 2988 5025 6
Size: 2982 2037 455
Fragments arranged by size:
2982 2037 455
BsaHI Gr'CG_yC
Cuts at: 2905 3153 3855 5351 2905
Size: 248 702 1496 3028
Fragments arranged by size:
3028 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2663 3184 3862 4136 4283 4694 2663
Size: 521 678 274 147 411 3443
Fragments arranged by size:
3443 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 733 763 733
Size: 30 5444
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 568 1051 1112 2431 568
Size: 483 61 1319 3611
Fragments arranged by size:
3611 1319 483 61
BseYI C'CCAG_C
Cuts at: 281 366 677 2301 2899 3657 4234 4730 281
Size: 85 311 1624 598 758 577 496 1025
Fragments arranged by size:
1624 1025 758 598 577 496 311 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1684 322
Size: 1362 4112
BsiEI CG_ry'CG
Cuts at: 104 2082 3016 3062 4270 104
Size: 1978 934 46 1208 1308
Fragments arranged by size:
1978 1308 1208 934 46
BsiHKAI G_wGCw'C
Cuts at: 329 1001 1196 2423 2488 3266 3456 4248 329
Size: 672 195 1227 65 778 190 792 1555
Fragments arranged by size:
1555 1227 792 778 672 195 190 65
BsmBI CGTCTCn'nnnn_
Cuts at: 703 703
Size: 5474
Bsp1286I G_dGCh'C
Cuts at: 329 374 688 908 1001 1196 1350 2423 2488
Size: 45 314 220 93 195 154 1073 65
Cuts at: 2488 3099 3192 3266 3456 3518 4248 329
Size: 611 93 74 190 62 730 1555
Fragments arranged by size:
1555 1073 730 611 314 220 195 190
154 93 93 74 65 62 45
BspEI T'CCGG_A
Cuts at: 4694 4694
Size: 5474
BspHI T'CATG_A
Cuts at: 4650 4650
Size: 5474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 720 2524 2738 3040 3421 720
Size: 1804 214 302 381 2773
Fragments arranged by size:
2773 1804 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3766 3820 5304 5469 50
Size: 3716 54 1484 165 55
Fragments arranged by size:
3716 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1281 1828 1991 3386 1281
Size: 547 163 1395 3369
Fragments arranged by size:
3369 1395 547 163
BsrFI r'CCGG_y
Cuts at: 974 2429 3472 3653 3862 974
Size: 1455 1043 181 209 2586
Fragments arranged by size:
2586 1455 1043 209 181
BsrGI T'GTAC_A
Cuts at: 446 446
Size: 5474
BssSI C'ACGA_G
Cuts at: 1696 3745 4103 1696
Size: 2049 358 3067
Fragments arranged by size:
3067 2049 358
BstAPI GCAn_nnn'nTGC
Cuts at: 309 825 2851 309
Size: 516 2026 2932
Fragments arranged by size:
2932 2026 516
BstBI TT'CG_AA
Cuts at: 3835 3835
Size: 5474
BstEII G'GTnAC_C
Cuts at: 1089 1089
Size: 5474
BstXI CCAn_nnnn'nTGG
Cuts at: 420 849 2283 420
Size: 429 1434 3611
Fragments arranged by size:
3611 1434 429
BstYI r'GATC_y
Cuts at: 1 246 650 983 1292 2450 2499 2966 2989
Size: 245 404 333 309 1158 49 467 23
Cuts at: 2989 3324 3570 4582 4668 4680 5026 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1158 1012 467 449 404 346 335 333
309 246 245 86 49 23 12
BstZ17I GTA'TAC
Cuts at: 2142 2142
Size: 5474
Bsu36I CC'TnA_GG
Cuts at: 646 1124 646
Size: 478 4996
BtgI C'CryG_G
Cuts at: 107 413 107
Size: 306 5168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3424 3605 3768 3424
Size: 181 163 5130
Fragments arranged by size:
5130 181 163
BtsI GCAGTG_nn'
Cuts at: 769 2717 769
Size: 1948 3526
DraI TTT'AAA
Cuts at: 2473 2473
Size: 5474
DraIII CAC_nnn'GTG
Cuts at: 333 333
Size: 5474
DrdI GACnn_nn'nnGTC
Cuts at: 3180 4038 3180
Size: 858 4616
EaeI y'GGCC_r
Cuts at: 174 794 890 953 1046 1544 1618 2125 2431
Size: 620 96 63 93 498 74 507 306
Cuts at: 2431 3059 3233 3624 3651 4996 174
Size: 628 174 391 27 1345 652
Fragments arranged by size:
1345 652 628 620 507 498 391 306
174 96 93 74 63 27
EagI C'GGCC_G
Cuts at: 3059 3059
Size: 5474
EarI CTCTTCn'nnn_
Cuts at: 3497 3707 3497
Size: 210 5264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 424 3682 3992 4138 5284 424
Size: 3258 310 146 1146 614
Fragments arranged by size:
3258 1146 614 310 146
EcoICRI GAG'CTC
Cuts at: 2421 2486 2421
Size: 65 5409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 373 376 476 517 703 760 1153 1338
Size: 63 3 100 41 186 57 393 185
Cuts at: 1338 1762 2404 3299 3731 4478 4746 310
Size: 424 642 895 432 747 268 1038
Fragments arranged by size:
1038 895 747 642 432 424 393 268
186 185 100 63 57 41 3
EcoNI CCTnn'n_nnAGG
Cuts at: 301 1684 301
Size: 1383 4091
EcoO109I rG'GnC_Cy
Cuts at: 294 905 1128 1677 1946 2292 2613 294
Size: 611 223 549 269 346 321 3155
Fragments arranged by size:
3155 611 549 346 321 269 223
EcoRI G'AATT_C
Cuts at: 2478 2478
Size: 5474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3493 3493
Size: 5474
FseI GG_CCGG'CC
Cuts at: 2433 2433
Size: 5474
FspI TGC'GCA
Cuts at: 3255 3255
Size: 5474
HaeII r_GCGC'y
Cuts at: 1639 2276 2908 3156 4178 5157 1639
Size: 637 632 248 1022 979 1956
Fragments arranged by size:
1956 1022 979 637 632 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 502 534 642 674 1063 1095 3316
Size: 32 359 32 108 32 389 32 2221
Cuts at: 3316 3346 3348 3378 111
Size: 30 2 30 2207
Fragments arranged by size:
2221 2207 389 359 108 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1056 2513 1056
Size: 1457 4017
KasI G'GCGC_C
Cuts at: 2904 3152 2904
Size: 248 5226
KpnI G_GTAC'C
Cuts at: 2494 2494
Size: 5474
MluI A'CGCG_T
Cuts at: 4907 4907
Size: 5474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4145 4329 4145
Size: 184 5290
MscI TGG'CCA
Cuts at: 176 1620 3235 176
Size: 1444 1615 2415
Fragments arranged by size:
2415 1615 1444
MslI CAynn'nnrTG
Cuts at: 112 193 1626 1874 2281 3463 3590 112
Size: 81 1433 248 407 1182 127 1996
Fragments arranged by size:
1996 1433 1182 407 248 127 81
MspA1I CmG'CkG
Cuts at: 407 428 496 672 681 822 888 1014 1626
Size: 21 68 176 9 141 66 126 612
Cuts at: 1626 2305 2585 2899 3259 4272 4517 407
Size: 679 280 314 360 1013 245 1364
Fragments arranged by size:
1364 1013 679 612 360 314 280 245
176 141 126 68 66 21 9
NaeI GCC'GGC
Cuts at: 2431 3655 2431
Size: 1224 4250
NarI GG'CG_CC
Cuts at: 2905 3153 2905
Size: 248 5226
NcoI C'CATG_G
Cuts at: 107 413 107
Size: 306 5168
NgoMIV G'CCGG_C
Cuts at: 2429 3653 2429
Size: 1224 4250
NspI r_CATG'y
Cuts at: 2334 2527 3027 3934 2334
Size: 193 500 907 3874
Fragments arranged by size:
3874 907 500 193
PciI A'CATG_T
Cuts at: 2330 3930 2330
Size: 1600 3874
PflMI CCAn_nnn'nTGG
Cuts at: 288 1997 288
Size: 1709 3765
PfoI T'CCnGG_A
Cuts at: 287 848 287
Size: 561 4913
PmeI GTTT'AAAC
Cuts at: 2473 2473
Size: 5474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2367 4613 4645 2335
Size: 32 2246 32 3164
Fragments arranged by size:
3164 2246 32 32
PpuMI rG'GwC_Cy
Cuts at: 1128 1946 1128
Size: 818 4656
PspOMI G'GGCC_C
Cuts at: 904 904
Size: 5474
PstI C_TGCA'G
Cuts at: 314 605 776 821 2256 2521 3206 314
Size: 291 171 45 1435 265 685 2582
Fragments arranged by size:
2582 1435 685 291 265 171 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5474
PvuII CAG'CTG
Cuts at: 496 681 822 888 1014 1626 2305 2899 3259
Size: 185 141 66 126 612 679 594 360
Cuts at: 3259 496
Size: 2711
Fragments arranged by size:
2711 679 612 594 360 185 141 126
66
RsrII CG'GwC_CG
Cuts at: 3669 3669
Size: 5474
SacI G_AGCT'C
Cuts at: 2423 2488 2423
Size: 65 5409
SalI G'TCGA_C
Cuts at: 2511 2511
Size: 5474
SapI GCTCTTCn'nnn_
Cuts at: 3497 3707 3497
Size: 210 5264
SbfI CC_TGCA'GG
Cuts at: 2521 2521
Size: 5474
ScaI AGT'ACT
Cuts at: 619 4991 619
Size: 4372 1102
SexAI A'CCwGG_T
Cuts at: 329 329
Size: 5474
SfcI C'TryA_G
Cuts at: 33 310 601 772 817 931 1749 2252 2517
Size: 277 291 171 45 114 818 503 265
Cuts at: 2517 3202 4195 4386 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 818 685 503 291 277 265
191 171 114 45
SfoI GGC'GCC
Cuts at: 2906 3154 2906
Size: 248 5226
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
SmaI CCC'GGG
Cuts at: 2496 2496
Size: 5474
SmlI C'TyrA_G
Cuts at: 1255 1861 2629 3544 4036 4298 1255
Size: 606 768 915 492 262 2431
Fragments arranged by size:
2431 915 768 606 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5474
SphI G_CATG'C
Cuts at: 2527 3027 2527
Size: 500 4974
StuI AGG'CCT
Cuts at: 745 745
Size: 5474
StyI C'CwwG_G
Cuts at: 107 138 413 1685 2608 2962 107
Size: 31 275 1272 923 354 2619
Fragments arranged by size:
2619 1272 923 354 275 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 295 2057 3057 3190 3649 3858 295
Size: 1762 1000 133 459 209 1911
Fragments arranged by size:
1911 1762 1000 459 209 133
TatI w'GTAC_w
Cuts at: 446 617 4989 446
Size: 171 4372 931
Fragments arranged by size:
4372 931 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2148 2180 2431 2463 4991 5023 2148
Size: 32 251 32 2528 32 2599
Fragments arranged by size:
2599 2528 251 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1491 2353 3271 1491
Size: 862 918 3694
Fragments arranged by size:
3694 918 862
XbaI T'CTAG_A
Cuts at: 2505 2505
Size: 5474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 285 661 1453 2969 285
Size: 376 792 1516 2790
Fragments arranged by size:
2790 1516 792 376
XmaI C'CCGG_G
Cuts at: 2494 2494
Size: 5474
XmnI GAAnn'nnTTC
Cuts at: 149 1717 149
Size: 1568 3906
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BssHII ClaI CspCI EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI