Restriction map for
pF1KB3253
(Circular) (Six-base) MAPSORT of: pF1KB3253.seq Check: 1421 from: 1 to: 4976
pF1KB3253 4976 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 2014 2014
Size: 4976
Acc65I G'GTAC_C
Cuts at: 1992 1992
Size: 4976
AclI AA'CG_TT
Cuts at: 4816 4816
Size: 4976
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 934 1360 2801 3233 3980 4248 315
Size: 619 426 1441 432 747 268 1043
Fragments arranged by size:
1441 1043 747 619 432 426 268
AflIII A'CryG_T
Cuts at: 1598 2328 3432 4409 1598
Size: 730 1104 977 2165
Fragments arranged by size:
2165 1104 977 730
AgeI A'CCGG_T
Cuts at: 3364 3364
Size: 4976
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1611 1643 1611
Size: 32 4944
AlwNI CAG_nnn'CTG
Cuts at: 1830 1927 2281 3848 1830
Size: 97 354 1567 2958
Fragments arranged by size:
2958 1567 354 97
ApaLI G'TGCA_C
Cuts at: 3746 3746
Size: 4976
ApoI r'AATT_y
Cuts at: 1240 1354 1980 4329 4585 1240
Size: 114 626 2349 256 1631
Fragments arranged by size:
2349 1631 626 256 114
AseI AT'TA_AT
Cuts at: 20 20
Size: 4976
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4976
AvaI C'yCGr_G
Cuts at: 1020 1996 1020
Size: 976 4000
AvrII C'CTAG_G
Cuts at: 1630 1630
Size: 4976
BamHI G'GATC_C
Cuts at: 2001 2001
Size: 4976
BanI G'GyrC_C
Cuts at: 746 1992 2406 2654 2689 746
Size: 1246 414 248 35 3033
Fragments arranged by size:
3033 1246 414 248 35
BanII G_rGCy'C
Cuts at: 1021 1451 1990 3020 1021
Size: 430 539 1030 2977
Fragments arranged by size:
2977 1030 539 430
BbeI G_GCGC'C
Cuts at: 2410 2658 2410
Size: 248 4728
BbsI GAAGACnn'nnnn_
Cuts at: 887 1056 1471 1502 887
Size: 169 415 31 4361
Fragments arranged by size:
4361 415 169 31
BbvCI CC'TCA_GC
Cuts at: 500 500
Size: 4976
BciVI GTATCCnnnnn_n'
Cuts at: 813 2874 3635 4976 813
Size: 2061 761 1341 813
Fragments arranged by size:
2061 1341 813 761
BclI T'GATC_A
Cuts at: 2496 2496
Size: 4976
BfrBI ATG'CAT
Cuts at: 433 433
Size: 4976
BglII A'GATC_T
Cuts at: 423 2491 4528 423
Size: 2068 2037 871
Fragments arranged by size:
2068 2037 871
BlpI GC'TnA_GC
Cuts at: 2088 2088
Size: 4976
Bme1580I G_kGCm'C
Cuts at: 2601 2694 3750 2601
Size: 93 1056 3827
Fragments arranged by size:
3827 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 661 2188 2269 2604 4748 661
Size: 1527 81 335 2144 889
Fragments arranged by size:
2144 1527 889 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1465 3343 1465
Size: 1878 3098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 772
Size: 4976
Bpu10I CC'TnA_GC
Cuts at: 500 500
Size: 4976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 2152 3031 3523 3821 1435
Size: 717 879 492 298 2590
Fragments arranged by size:
2590 879 717 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4680 4680
Size: 4976
BsaAI yAC'GTr
Cuts at: 2329 2329
Size: 4976
BsaBI GATnn'nnATC
Cuts at: 6 2490 4527 6
Size: 2484 2037 455
Fragments arranged by size:
2484 2037 455
BsaHI Gr'CG_yC
Cuts at: 2407 2655 3357 4853 2407
Size: 248 702 1496 2530
Fragments arranged by size:
2530 1496 702 248
BsaWI w'CCGG_w
Cuts at: 119 647 1006 2165 2686 3364 3638 3785 4196
Size: 528 359 1159 521 678 274 147 411
Cuts at: 4196 119
Size: 899
Fragments arranged by size:
1159 899 678 528 521 411 359 274
147
BseYI C'CCAG_C
Cuts at: 2401 3159 3736 4232 2401
Size: 758 577 496 3145
Fragments arranged by size:
3145 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2518 2564 3772 104
Size: 2414 46 1208 1308
Fragments arranged by size:
2414 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1021 1451 1990 2768 2958 3750 1021
Size: 430 539 778 190 792 2247
Fragments arranged by size:
2247 792 778 539 430 190
BsmI GAATG_Cn'
Cuts at: 843 843
Size: 4976
Bsp1286I G_dGCh'C
Cuts at: 1021 1451 1990 2601 2694 2768 2958 3020 3750
Size: 430 539 611 93 74 190 62 730
Cuts at: 3750 1021
Size: 2247
Fragments arranged by size:
2247 730 611 539 430 190 93 74
62
BspEI T'CCGG_A
Cuts at: 119 647 4196 119
Size: 528 3549 899
Fragments arranged by size:
3549 899 528
BspHI T'CATG_A
Cuts at: 935 4152 935
Size: 3217 1759
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1935 2026 2240 2542 2923 1935
Size: 91 214 302 381 3988
Fragments arranged by size:
3988 381 302 214 91
BsrBI CCG'CTC
Cuts at: 50 3268 3322 4806 4971 50
Size: 3218 54 1484 165 55
Fragments arranged by size:
3218 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 848 2888 848
Size: 2040 2936
BsrFI r'CCGG_y
Cuts at: 2974 3155 3364 2974
Size: 181 209 4586
Fragments arranged by size:
4586 209 181
BssSI C'ACGA_G
Cuts at: 3247 3605 3247
Size: 358 4618
BstAPI GCAn_nnn'nTGC
Cuts at: 1830 2353 1830
Size: 523 4453
BstBI TT'CG_AA
Cuts at: 1854 3337 1854
Size: 1483 3493
BstEII G'GTnAC_C
Cuts at: 1270 1270
Size: 4976
BstXI CCAn_nnnn'nTGG
Cuts at: 750 1267 750
Size: 517 4459
BstYI r'GATC_y
Cuts at: 1 423 1223 1379 2001 2468 2491 2826 3072
Size: 422 800 156 622 467 23 335 246
Cuts at: 3072 4084 4170 4182 4528 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 800 622 467 449 422 346 335
246 156 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4976
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2926 3107 3270 2926
Size: 181 163 4632
Fragments arranged by size:
4632 181 163
BtsI GCAGTG_nn'
Cuts at: 1621 2219 1621
Size: 598 4378
DraI TTT'AAA
Cuts at: 1818 1975 1818
Size: 157 4819
DrdI GACnn_nn'nnGTC
Cuts at: 2682 3540 2682
Size: 858 4118
EaeI y'GGCC_r
Cuts at: 845 1070 1257 1480 2561 2735 3126 3153 4498
Size: 225 187 223 1081 174 391 27 1345
Cuts at: 4498 845
Size: 1323
Fragments arranged by size:
1345 1323 1081 391 225 223 187 174
27
EagI C'GGCC_G
Cuts at: 2561 2561
Size: 4976
EarI CTCTTCn'nnn_
Cuts at: 1439 1856 1901 2999 3209 1439
Size: 417 45 1098 210 3206
Fragments arranged by size:
3206 1098 417 210 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3184 3494 3640 4786 3184
Size: 310 146 1146 3374
Fragments arranged by size:
3374 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1019 1449 1988 1019
Size: 430 539 4007
Fragments arranged by size:
4007 539 430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 772 934 1360 2801 3233 3980 4248 315
Size: 457 162 426 1441 432 747 268 1043
Fragments arranged by size:
1441 1043 747 457 432 426 268 162
EcoNI CCTnn'n_nnAGG
Cuts at: 1179 1756 1179
Size: 577 4399
EcoO109I rG'GnC_Cy
Cuts at: 2115 2115
Size: 4976
EcoRI G'AATT_C
Cuts at: 1354 1980 1354
Size: 626 4350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1387 1527 1982 2995 1387
Size: 140 455 1013 3368
Fragments arranged by size:
3368 1013 455 140
FspI TGC'GCA
Cuts at: 2757 2757
Size: 4976
HaeII r_GCGC'y
Cuts at: 2410 2658 3680 4659 2410
Size: 248 1022 979 2727
Fragments arranged by size:
2727 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 988 1020 1210 1242 1693 1725 2818 2848 2850
Size: 32 190 32 451 32 1093 30 2
Cuts at: 2850 2880 988
Size: 30 3084
Fragments arranged by size:
3084 1093 451 190 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2015 2015
Size: 4976
KasI G'GCGC_C
Cuts at: 2406 2654 2406
Size: 248 4728
KpnI G_GTAC'C
Cuts at: 1996 1996
Size: 4976
MluI A'CGCG_T
Cuts at: 4409 4409
Size: 4976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 894 1244 3647 3831 894
Size: 350 2403 184 2039
Fragments arranged by size:
2403 2039 350 184
MscI TGG'CCA
Cuts at: 847 1072 1259 1482 2737 847
Size: 225 187 223 1255 3086
Fragments arranged by size:
3086 1255 225 223 187
MslI CAynn'nnrTG
Cuts at: 2965 3092 2965
Size: 127 4849
MspA1I CmG'CkG
Cuts at: 859 2087 2401 2761 3774 4019 859
Size: 1228 314 360 1013 245 1816
Fragments arranged by size:
1816 1228 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3157 3157
Size: 4976
NarI GG'CG_CC
Cuts at: 2407 2655 2407
Size: 248 4728
NcoI C'CATG_G
Cuts at: 107 107
Size: 4976
NgoMIV G'CCGG_C
Cuts at: 3155 3155
Size: 4976
NsiI A_TGCA'T
Cuts at: 435 435
Size: 4976
NspI r_CATG'y
Cuts at: 1602 2029 2529 3436 1602
Size: 427 500 907 3142
Fragments arranged by size:
3142 907 500 427
PasI CC'CwG_GG
Cuts at: 1152 1152
Size: 4976
PciI A'CATG_T
Cuts at: 1598 3432 1598
Size: 1834 3142
PflMI CCAn_nnn'nTGG
Cuts at: 730 730
Size: 4976
PmeI GTTT'AAAC
Cuts at: 1975 1975
Size: 4976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 4115
Size: 32 4944
PshAI GACnn'nnGTC
Cuts at: 130 130
Size: 4976
PsiI TTA'TAA
Cuts at: 443 443
Size: 4976
PstI C_TGCA'G
Cuts at: 1062 2023 2708 1062
Size: 961 685 3330
Fragments arranged by size:
3330 961 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4976
PvuII CAG'CTG
Cuts at: 859 2401 2761 859
Size: 1542 360 3074
Fragments arranged by size:
3074 1542 360
RsrII CG'GwC_CG
Cuts at: 3171 3171
Size: 4976
SacI G_AGCT'C
Cuts at: 1021 1451 1990 1021
Size: 430 539 4007
Fragments arranged by size:
4007 539 430
SalI G'TCGA_C
Cuts at: 2013 2013
Size: 4976
SapI GCTCTTCn'nnn_
Cuts at: 1439 1856 2999 3209 1439
Size: 417 1143 210 3206
Fragments arranged by size:
3206 1143 417 210
SbfI CC_TGCA'GG
Cuts at: 2023 2023
Size: 4976
ScaI AGT'ACT
Cuts at: 1510 4493 1510
Size: 2983 1993
SfcI C'TryA_G
Cuts at: 33 1058 1179 1524 1859 2019 2704 3697 3888
Size: 1025 121 345 335 160 685 993 191
Cuts at: 3888 33
Size: 1121
Fragments arranged by size:
1121 1025 993 685 345 335 191 160
121
SfoI GGC'GCC
Cuts at: 2408 2656 2408
Size: 248 4728
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4976
SmaI CCC'GGG
Cuts at: 1998 1998
Size: 4976
SmlI C'TyrA_G
Cuts at: 1450 2131 3046 3538 3800 1450
Size: 681 915 492 262 2626
Fragments arranged by size:
2626 915 681 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4976
SphI G_CATG'C
Cuts at: 2029 2529 2029
Size: 500 4476
SspI AAT'ATT
Cuts at: 897 927 897
Size: 30 4946
StyI C'CwwG_G
Cuts at: 107 128 1419 1630 1757 2110 2464 107
Size: 21 1291 211 127 353 354 2619
Fragments arranged by size:
2619 1291 354 353 211 127 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 170 1345 2559 2692 3151 3360 170
Size: 1175 1214 133 459 209 1786
Fragments arranged by size:
1786 1214 1175 459 209 133
TatI w'GTAC_w
Cuts at: 1508 4491 1508
Size: 2983 1993
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1303 1335 4493 4525 1303
Size: 32 3158 32 1754
Fragments arranged by size:
3158 1754 32 32
Tth111I GACn'n_nGTC
Cuts at: 2773 2773
Size: 4976
XbaI T'CTAG_A
Cuts at: 2007 2007
Size: 4976
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 533 2471 533
Size: 1938 3038
XmaI C'CCGG_G
Cuts at: 1996 1996
Size: 4976
XmnI GAAnn'nnTTC
Cuts at: 926 1337 1367 926
Size: 411 30 4535
Fragments arranged by size:
4535 411 30
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI BaeI BcgI BglI BmgBI BmtI BsaXI BseRI
BsgI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI