Restriction map for
pF1KB3248
(Circular) (Six-base) MAPSORT of: pF1KB3248.seq Check: 2115 from: 1 to: 3743
pF1KB3248 3743 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 781 781
Size: 3743
Acc65I G'GTAC_C
Cuts at: 759 759
Size: 3743
AclI AA'CG_TT
Cuts at: 3583 3583
Size: 3743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1568 2000 2353 3015 298
Size: 1270 432 353 662 1026
Fragments arranged by size:
1270 1026 662 432 353
AflIII A'CryG_T
Cuts at: 1095 2895 3176 1095
Size: 1800 281 1662
Fragments arranged by size:
1800 1662 281
AlwNI CAG_nnn'CTG
Cuts at: 1048 2486 1048
Size: 1438 2305
ApaLI G'TGCA_C
Cuts at: 2581 2581
Size: 3743
ApoI r'AATT_y
Cuts at: 229 439 451 747 3096 3352 229
Size: 210 12 296 2349 256 620
Fragments arranged by size:
2349 620 296 256 210 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
AvaI C'yCGr_G
Cuts at: 647 763 647
Size: 116 3627
BamHI G'GATC_C
Cuts at: 768 768
Size: 3743
BanI G'GyrC_C
Cuts at: 759 1173 1421 1456 759
Size: 414 248 35 3046
Fragments arranged by size:
3046 414 248 35
BanII G_rGCy'C
Cuts at: 757 1787 757
Size: 1030 2713
BbeI G_GCGC'C
Cuts at: 1177 1425 1177
Size: 248 3495
BciVI GTATCCnnnnn_n'
Cuts at: 1641 2697 3743 1641
Size: 1056 1046 1641
Fragments arranged by size:
1641 1056 1046
BclI T'GATC_A
Cuts at: 1263 1263
Size: 3743
BglII A'GATC_T
Cuts at: 1258 3295 1258
Size: 2037 1706
BlpI GC'TnA_GC
Cuts at: 855 855
Size: 3743
Bme1580I G_kGCm'C
Cuts at: 1368 1461 2585 1368
Size: 93 1124 2526
Fragments arranged by size:
2526 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 955 1036 1371 3515 955
Size: 81 335 2144 1183
Fragments arranged by size:
2144 1183 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 3743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 582
Size: 3743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1798 2512 2810 919
Size: 879 714 298 1852
Fragments arranged by size:
1852 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3447 3447
Size: 3743
BsaAI yAC'GTr
Cuts at: 1096 1096
Size: 3743
BsaBI GATnn'nnATC
Cuts at: 6 1257 3294 6
Size: 1251 2037 455
Fragments arranged by size:
2037 1251 455
BsaHI Gr'CG_yC
Cuts at: 1174 1422 2124 3620 1174
Size: 248 702 1496 1297
Fragments arranged by size:
1496 1297 702 248
BsaWI w'CCGG_w
Cuts at: 932 1453 2131 2542 2689 2963 932
Size: 521 678 411 147 274 1712
Fragments arranged by size:
1712 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 154
Size: 3743
BseYI C'CCAG_C
Cuts at: 1168 1926 2591 2999 1168
Size: 758 665 408 1912
Fragments arranged by size:
1912 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1285 1331 2561 104
Size: 1181 46 1230 1286
Fragments arranged by size:
1286 1230 1181 46
BsiHKAI G_wGCw'C
Cuts at: 757 1535 1725 2585 757
Size: 778 190 860 1915
Fragments arranged by size:
1915 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 757 1368 1461 1535 1725 1787 2585 757
Size: 611 93 74 190 62 798 1915
Fragments arranged by size:
1915 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 225 2175 225
Size: 1950 1793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 793 1007 1309 1690 793
Size: 214 302 381 2846
Fragments arranged by size:
2846 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2035 2089 3573 3738 50
Size: 1985 54 1484 165 55
Fragments arranged by size:
1985 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1655 1655
Size: 3743
BsrFI r'CCGG_y
Cuts at: 1741 1922 1741
Size: 181 3562
BssSI C'ACGA_G
Cuts at: 2014 2722 2014
Size: 708 3035
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 1120
Size: 3743
BstBI TT'CG_AA
Cuts at: 2104 2104
Size: 3743
BstEII G'GTnAC_C
Cuts at: 114 114
Size: 3743
BstYI r'GATC_y
Cuts at: 1 267 768 1235 1258 1593 1839 2145 2157
Size: 266 501 467 23 335 246 306 12
Cuts at: 2157 2243 3295 1
Size: 86 1052 449
Fragments arranged by size:
1052 501 467 449 335 306 266 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 162 1693 1874 2037 162
Size: 1531 181 163 1868
Fragments arranged by size:
1868 1531 181 163
BtsI GCAGTG_nn'
Cuts at: 986 986
Size: 3743
DraI TTT'AAA
Cuts at: 742 742
Size: 3743
DrdI GACnn_nn'nnGTC
Cuts at: 1449 2793 1449
Size: 1344 2399
EaeI y'GGCC_r
Cuts at: 444 1328 1502 1893 1920 3265 444
Size: 884 174 391 27 1345 922
Fragments arranged by size:
1345 922 884 391 174 27
EagI C'GGCC_G
Cuts at: 1328 1328
Size: 3743
EarI CTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1951 2693 2839 3553 1951
Size: 742 146 714 2141
Fragments arranged by size:
2141 742 714 146
EcoICRI GAG'CTC
Cuts at: 755 755
Size: 3743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 582 1568 2000 2353 3015 298
Size: 284 986 432 353 662 1026
Fragments arranged by size:
1026 986 662 432 353 284
EcoO109I rG'GnC_Cy
Cuts at: 664 882 664
Size: 218 3525
EcoRI G'AATT_C
Cuts at: 229 747 229
Size: 518 3225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1762 1762
Size: 3743
FspI TGC'GCA
Cuts at: 1524 1524
Size: 3743
HaeII r_GCGC'y
Cuts at: 1177 1425 2655 3426 1177
Size: 248 1230 771 1494
Fragments arranged by size:
1494 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1585 1615 1617 1647 1585
Size: 30 2 30 3681
Fragments arranged by size:
3681 30 30 2
HincII GTy'rAC
Cuts at: 782 782
Size: 3743
KasI G'GCGC_C
Cuts at: 1173 1421 1173
Size: 248 3495
KpnI G_GTAC'C
Cuts at: 763 763
Size: 3743
MluI A'CGCG_T
Cuts at: 3176 3176
Size: 3743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2502 2686 2502
Size: 184 3559
MscI TGG'CCA
Cuts at: 446 1504 446
Size: 1058 2685
MslI CAynn'nnrTG
Cuts at: 112 1732 1859 112
Size: 1620 127 1996
Fragments arranged by size:
1996 1620 127
MspA1I CmG'CkG
Cuts at: 854 1168 1528 2312 2557 854
Size: 314 360 784 245 2040
Fragments arranged by size:
2040 784 360 314 245
NaeI GCC'GGC
Cuts at: 1924 1924
Size: 3743
NarI GG'CG_CC
Cuts at: 1174 1422 1174
Size: 248 3495
NgoMIV G'CCGG_C
Cuts at: 1922 1922
Size: 3743
NspI r_CATG'y
Cuts at: 151 420 796 1296 2899 151
Size: 269 376 500 1603 995
Fragments arranged by size:
1603 995 500 376 269
PciI A'CATG_T
Cuts at: 2895 2895
Size: 3743
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2189 2221 2189
Size: 32 3711
PpuMI rG'GwC_Cy
Cuts at: 664 664
Size: 3743
PspXI vC'TCGA_Gb
Cuts at: 647 647
Size: 3743
PstI C_TGCA'G
Cuts at: 146 309 790 1475 146
Size: 163 481 685 2414
Fragments arranged by size:
2414 685 481 163
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3743
PvuII CAG'CTG
Cuts at: 1168 1528 1168
Size: 360 3383
RsrII CG'GwC_CG
Cuts at: 1938 1938
Size: 3743
SacI G_AGCT'C
Cuts at: 757 757
Size: 3743
SalI G'TCGA_C
Cuts at: 780 780
Size: 3743
SanDI GG'GwC_CC
Cuts at: 664 664
Size: 3743
SapI GCTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
SbfI CC_TGCA'GG
Cuts at: 790 790
Size: 3743
ScaI AGT'ACT
Cuts at: 3260 3260
Size: 3743
SfcI C'TryA_G
Cuts at: 33 142 305 622 786 1471 2439 2630 33
Size: 109 163 317 164 685 968 191 1146
Fragments arranged by size:
1146 968 685 317 191 164 163 109
SfoI GGC'GCC
Cuts at: 1175 1423 1175
Size: 248 3495
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
SmaI CCC'GGG
Cuts at: 765 765
Size: 3743
SmlI C'TyrA_G
Cuts at: 647 898 1813 2527 2789 647
Size: 251 915 714 262 1601
Fragments arranged by size:
1601 915 714 262 251
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3743
SphI G_CATG'C
Cuts at: 796 1296 796
Size: 500 3243
StyI C'CwwG_G
Cuts at: 877 1231 877
Size: 354 3389
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 102 1326 1459 1918 2127 102
Size: 1224 133 459 209 1718
Fragments arranged by size:
1718 1224 459 209 133
TatI w'GTAC_w
Cuts at: 328 3258 328
Size: 2930 813
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 669 701 3260 3292 669
Size: 32 2559 32 1120
Fragments arranged by size:
2559 1120 32 32
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3743
XbaI T'CTAG_A
Cuts at: 774 774
Size: 3743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1238 1238
Size: 3743
XhoI C'TCGA_G
Cuts at: 647 647
Size: 3743
XmaI C'CCGG_G
Cuts at: 763 763
Size: 3743
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI