Restriction map for
pF1KB3244
(Circular) (Six-base) MAPSORT of: pF1KB3244.seq Check: 9748 from: 1 to: 9599
pF1KB3244 9599 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3382 4346 5150 5590 3382
Size: 964 804 440 7391
Fragments arranged by size:
7391 964 804 440
AatII G_ACGT'C
Cuts at: 5978 5978
Size: 9599
AccI GT'mk_AC
Cuts at: 172 464 1877 2744 6637 172
Size: 292 1413 867 3893 3134
Fragments arranged by size:
3893 3134 1413 867 292
Acc65I G'GTAC_C
Cuts at: 582 714 1754 3164 5319 6615 582
Size: 132 1040 1410 2155 1296 3566
Fragments arranged by size:
3566 2155 1410 1296 1040 132
AclI AA'CG_TT
Cuts at: 9439 9439
Size: 9599
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1083 1121 1396 1909 2963 3056 4361 4603 5818
Size: 38 275 513 1054 93 1305 242 1215
Cuts at: 5818 6181 7424 7856 8209 8871 1083
Size: 363 1243 432 353 662 1811
Fragments arranged by size:
1811 1305 1243 1215 1054 662 513 432
363 353 275 242 93 38
AfeI AGC'GCT
Cuts at: 3244 4741 3244
Size: 1497 8102
AflII C'TTAA_G
Cuts at: 762 762
Size: 9599
AflIII A'CryG_T
Cuts at: 2870 3570 6951 8751 9032 2870
Size: 700 3381 1800 281 3437
Fragments arranged by size:
3437 3381 1800 700 281
AhdI GACnn_n'nnGTC
Cuts at: 3132 3132
Size: 9599
AleI CACnn'nnGTG
Cuts at: 401 1621 4414 5164 6148 6337 401
Size: 1220 2793 750 984 189 3663
Fragments arranged by size:
3663 2793 1220 984 750 189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2077 2179 3545 2077
Size: 102 1366 8131
Fragments arranged by size:
8131 1366 102
AlwNI CAG_nnn'CTG
Cuts at: 398 1443 1741 2598 4390 4482 4492 5316 5392
Size: 1045 298 857 1792 92 10 824 76
Cuts at: 5392 5628 6087 6904 8342 398
Size: 236 459 817 1438 1655
Fragments arranged by size:
1792 1655 1438 1045 857 824 817 459
298 236 92 76 10
ApaLI G'TGCA_C
Cuts at: 1078 4212 4333 4747 8437 1078
Size: 3134 121 414 3690 2240
Fragments arranged by size:
3690 3134 2240 414 121
ApoI r'AATT_y
Cuts at: 1339 1713 2767 6301 6603 8952 9208 1339
Size: 374 1054 3534 302 2349 256 1730
Fragments arranged by size:
3534 2349 1730 1054 374 302 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9599
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9599
AvaI C'yCGr_G
Cuts at: 217 342 470 1350 1698 2932 3130 5325 6437
Size: 125 128 880 348 1234 198 2195 1112
Cuts at: 6437 6619 217
Size: 182 3197
Fragments arranged by size:
3197 2195 1234 1112 880 348 198 182
128 125
AvrII C'CTAG_G
Cuts at: 4236 4236
Size: 9599
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 698 731 3156 3189 698
Size: 33 2425 33 7108
Fragments arranged by size:
7108 2425 33 33
BamHI G'GATC_C
Cuts at: 638 6510 6624 638
Size: 5872 114 3613
Fragments arranged by size:
5872 3613 114
BanI G'GyrC_C
Cuts at: 582 628 714 1754 2686 3025 3164 3705 4495
Size: 46 86 1040 932 339 139 541 790
Cuts at: 4495 4555 5260 5319 5578 5990 6231 6286 6615
Size: 60 705 59 259 412 241 55 329
Cuts at: 6615 7029 7277 7312 582
Size: 414 248 35 2869
Fragments arranged by size:
2869 1040 932 790 705 541 414 412
339 329 259 248 241 139 86 60
59 55 46 35
BanII G_rGCy'C
Cuts at: 961 1035 1309 1357 1801 1829 2072 2405 2933
Size: 74 274 48 444 28 243 333 528
Cuts at: 2933 3122 6613 7643 961
Size: 189 3491 1030 2917
Fragments arranged by size:
3491 2917 1030 528 444 333 274 243
189 74 48 28
BbeI G_GCGC'C
Cuts at: 4499 7033 7281 4499
Size: 2534 248 6817
Fragments arranged by size:
6817 2534 248
BbvCI CC'TCA_GC
Cuts at: 701 5372 5385 5513 701
Size: 4671 13 128 4787
Fragments arranged by size:
4787 4671 128 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 668 702 4948 4982 668
Size: 34 4246 34 5285
Fragments arranged by size:
5285 4246 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 816 7497 8553 9599 816
Size: 6681 1056 1046 816
Fragments arranged by size:
6681 1056 1046 816
BclI T'GATC_A
Cuts at: 1403 2289 6044 7119 1403
Size: 886 3755 1075 3883
Fragments arranged by size:
3883 3755 1075 886
BglI GCCn_nnn'nGGC
Cuts at: 112 895 1672 2005 6274 112
Size: 783 777 333 4269 3437
Fragments arranged by size:
4269 3437 783 777 333
BglII A'GATC_T
Cuts at: 251 2897 7114 9151 251
Size: 2646 4217 2037 699
Fragments arranged by size:
4217 2646 2037 699
BlpI GC'TnA_GC
Cuts at: 3196 3829 4727 5086 5893 6711 3196
Size: 633 898 359 807 818 6084
Fragments arranged by size:
6084 898 818 807 633 359
Bme1580I G_kGCm'C
Cuts at: 633 1082 1618 2689 3028 4042 4193 4216 4337
Size: 449 536 1071 339 1014 151 23 121
Cuts at: 4337 4558 4751 5263 7224 7317 8441 633
Size: 221 193 512 1961 93 1124 1791
Fragments arranged by size:
1961 1791 1124 1071 1014 536 512 449
339 221 193 151 121 93 23
BmgBI CAC'GTC
Cuts at: 3327 3744 3327
Size: 417 9182
BmrI ACTGGGnnnn_n'
Cuts at: 791 1038 2608 3616 3782 6198 6811 6892 7227
Size: 247 1570 1008 166 2416 613 81 335
Cuts at: 7227 9371 791
Size: 2144 1019
Fragments arranged by size:
2416 2144 1570 1019 1008 613 335 247
166 81
BmtI G_CTAG'C
Cuts at: 2047 4072 2047
Size: 2025 7574
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1029 1845 4623 4929 5550 7966 1029
Size: 816 2778 306 621 2416 2662
Fragments arranged by size:
2778 2662 2416 816 621 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 1355 3259 3475 3707 3943 4081 4888 5200
Size: 935 1904 216 232 236 138 807 312
Cuts at: 5200 6023 420
Size: 823 3996
Fragments arranged by size:
3996 1904 935 823 807 312 236 232
216 138
Bpu10I CC'TnA_GC
Cuts at: 701 885 1308 2066 2071 2152 3250 3314 4852
Size: 184 423 758 5 81 1098 64 1538
Cuts at: 4852 4904 5372 5385 5513 701
Size: 52 468 13 128 4787
Fragments arranged by size:
4787 1538 1098 758 468 423 184 128
81 64 52 13 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 3802 6775 7654 8368 8666 646
Size: 3156 2973 879 714 298 1579
Fragments arranged by size:
3156 2973 1579 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 325 509 597 760 1621 3227 4594 5035 9303
Size: 184 88 163 861 1606 1367 441 4268
Cuts at: 9303 325
Size: 621
Fragments arranged by size:
4268 1606 1367 861 621 441 184 163
88
BsaAI yAC'GTr
Cuts at: 354 1501 1949 3571 4761 6228 6952 354
Size: 1147 448 1622 1190 1467 724 3001
Fragments arranged by size:
3001 1622 1467 1190 1147 724 448
BsaBI GATnn'nnATC
Cuts at: 6 2920 7113 9150 6
Size: 2914 4193 2037 455
Fragments arranged by size:
4193 2914 2037 455
BsaHI Gr'CG_yC
Cuts at: 1928 2741 3986 4128 4454 4496 5975 7030 7278
Size: 813 1245 142 326 42 1479 1055 248
Cuts at: 7278 7980 9476 1928
Size: 702 1496 2051
Fragments arranged by size:
2051 1496 1479 1245 1055 813 702 326
248 142 42
BsaWI w'CCGG_w
Cuts at: 6788 7309 7987 8398 8545 8819 6788
Size: 521 678 411 147 274 7568
Fragments arranged by size:
7568 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 118 148 772 802 1676 1706 1894 1924 3689
Size: 30 624 30 874 30 188 30 1765
Cuts at: 3689 3719 4987 5017 5173 5203 5873 5903 118
Size: 30 1268 30 156 30 670 30 3814
Fragments arranged by size:
3814 1765 1268 874 670 624 188 156
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1025 1837 2251 4033 4789 5011 5530 6127 6331
Size: 812 414 1782 756 222 519 597 204
Cuts at: 6331 1025
Size: 4293
Fragments arranged by size:
4293 1782 812 756 597 519 414 222
204
BseYI C'CCAG_C
Cuts at: 391 510 1192 1749 1845 2110 2459 3021 4117
Size: 119 682 557 96 265 349 562 1096
Cuts at: 4117 4399 5200 5877 5996 6052 6117 6238 6582
Size: 282 801 677 119 56 65 121 344
Cuts at: 6582 7024 7782 8447 8855 391
Size: 442 758 665 408 1135
Fragments arranged by size:
1135 1096 801 758 682 677 665 562
557 442 408 349 344 282 265 121
119 119 96 65 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1447 1793 2413 5632 5656 5791 5971 1447
Size: 346 620 3219 24 135 180 5075
Fragments arranged by size:
5075 3219 620 346 180 135 24
BsiEI CG_ry'CG
Cuts at: 104 2418 7141 7187 8417 104
Size: 2314 4723 46 1230 1286
Fragments arranged by size:
4723 2314 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 263 1082 1124 1829 2072 2405 2566 2933 3122
Size: 819 42 705 243 333 161 367 189
Cuts at: 3122 4216 4337 4751 5839 5894 5987 6074 6344
Size: 1094 121 414 1088 55 93 87 270
Cuts at: 6344 6613 7391 7581 8441 263
Size: 269 778 190 860 1421
Fragments arranged by size:
1421 1094 1088 860 819 778 705 414
367 333 270 269 243 190 189 161
121 93 87 55 42
BsiWI C'GTAC_G
Cuts at: 3567 3567
Size: 9599
BsmI GAATG_Cn'
Cuts at: 1974 3393 3399 3565 1974
Size: 1419 6 166 8008
Fragments arranged by size:
8008 1419 166 6
BsmBI CGTCTCn'nnnn_
Cuts at: 1919 3273 4135 1919
Size: 1354 862 7383
Fragments arranged by size:
7383 1354 862
Bsp1286I G_dGCh'C
Cuts at: 263 633 961 1035 1082 1124 1309 1357 1618
Size: 370 328 74 47 42 185 48 261
Cuts at: 1618 1801 1829 2072 2405 2566 2689 2933 3028
Size: 183 28 243 333 161 123 244 95
Cuts at: 3028 3122 4042 4193 4216 4337 4558 4751 5263
Size: 94 920 151 23 121 221 193 512
Cuts at: 5263 5839 5894 5987 6074 6344 6613 7224 7317
Size: 576 55 93 87 270 269 611 93
Cuts at: 7317 7391 7581 7643 8441 263
Size: 74 190 62 798 1421
Fragments arranged by size:
1421 920 798 611 576 512 370 333
328 270 269 261 244 243 221 193
190 185 183 161 151 123 121 95
94 93 93 87 74 74 62 55
48 47 42 28 23
BspHI T'CATG_A
Cuts at: 6431 8031 6431
Size: 1600 7999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2510 3382 4346 4831 5108 5150 5301 5590 6649
Size: 872 964 485 277 42 151 289 1059
Cuts at: 6649 6863 7165 7546 2510
Size: 214 302 381 4563
Fragments arranged by size:
4563 1059 964 872 485 381 302 289
277 214 151 42
BsrBI CCG'CTC
Cuts at: 50 1224 3200 3459 5781 7891 7945 9429 9594
Size: 1174 1976 259 2322 2110 54 1484 165
Cuts at: 9594 50
Size: 55
Fragments arranged by size:
2322 2110 1976 1484 1174 259 165 55
54
BsrDI GCAATG_nn'
Cuts at: 3527 7511 3527
Size: 3984 5615
BsrFI r'CCGG_y
Cuts at: 199 3784 4124 5081 7597 7778 199
Size: 3585 340 957 2516 181 2020
Fragments arranged by size:
3585 2516 2020 957 340 181
BsrGI T'GTAC_A
Cuts at: 6551 6551
Size: 9599
BssSI C'ACGA_G
Cuts at: 1837 7870 8578 1837
Size: 6033 708 2858
Fragments arranged by size:
6033 2858 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1780 5112 6471 6976 1780
Size: 3332 1359 505 4403
Fragments arranged by size:
4403 3332 1359 505
BstBI TT'CG_AA
Cuts at: 2709 7960 2709
Size: 5251 4348
BstEII G'GTnAC_C
Cuts at: 778 3420 6154 778
Size: 2642 2734 4223
Fragments arranged by size:
4223 2734 2642
BstXI CCAn_nnnn'nTGG
Cuts at: 157 991 1028 2985 3453 3606 6582 157
Size: 834 37 1957 468 153 2976 3174
Fragments arranged by size:
3174 2976 1957 834 468 153 37
BstYI r'GATC_y
Cuts at: 1 251 638 674 857 2603 2897 3845 4778
Size: 250 387 36 183 1746 294 948 933
Cuts at: 4778 5175 6510 6624 7091 7114 7449 7695 8001
Size: 397 1335 114 467 23 335 246 306
Cuts at: 8001 8013 8099 9151 1
Size: 12 86 1052 449
Fragments arranged by size:
1746 1335 1052 948 933 467 449 397
387 335 306 294 250 246 183 114
86 36 23 12
BtgI C'CryG_G
Cuts at: 107 607 990 2478 2960 2990 3158 3284 3335
Size: 500 383 1488 482 30 168 126 51
Cuts at: 3335 4412 4559 4703 5433 5867 6282 107
Size: 1077 147 144 730 434 415 3424
Fragments arranged by size:
3424 1488 1077 730 500 482 434 415
383 168 147 144 126 51 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2433 3348 7549 7730 7893 2433
Size: 915 4201 181 163 4139
Fragments arranged by size:
4201 4139 915 181 163
BtsI GCAGTG_nn'
Cuts at: 1015 2621 3016 3440 5755 6014 6027 6842 1015
Size: 1606 395 424 2315 259 13 815 3772
Fragments arranged by size:
3772 2315 1606 815 424 395 259 13
ClaI AT'CG_AT
Cuts at: 4202 5303 4202
Size: 1101 8498
DraI TTT'AAA
Cuts at: 6598 6598
Size: 9599
DraIII CAC_nnn'GTG
Cuts at: 2298 3610 2298
Size: 1312 8287
DrdI GACnn_nn'nnGTC
Cuts at: 136 4630 7305 8649 136
Size: 4494 2675 1344 1086
Fragments arranged by size:
4494 2675 1344 1086
EaeI y'GGCC_r
Cuts at: 1326 1445 1997 2647 2987 3629 3782 4172 4562
Size: 119 552 650 340 642 153 390 390
Cuts at: 4562 5353 5457 6017 7184 7358 7749 7776 9121
Size: 791 104 560 1167 174 391 27 1345
Cuts at: 9121 1326
Size: 1804
Fragments arranged by size:
1804 1345 1167 791 650 642 560 552
391 390 390 340 174 153 119 104
27
EagI C'GGCC_G
Cuts at: 7184 7184
Size: 9599
EarI CTCTTCn'nnn_
Cuts at: 763 1045 2191 4140 7622 7832 763
Size: 282 1146 1949 3482 210 2530
Fragments arranged by size:
3482 2530 1949 1146 282 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5257 6520 7807 8549 8695 9409 5257
Size: 1263 1287 742 146 714 5447
Fragments arranged by size:
5447 1287 1263 742 714 146
EcoICRI GAG'CTC
Cuts at: 1827 2070 2403 2931 3120 6611 1827
Size: 243 333 528 189 3491 4815
Fragments arranged by size:
4815 3491 528 333 243 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 1083 1121 1355 1396 1909 2963 3056 3259
Size: 663 38 234 41 513 1054 93 203
Cuts at: 3259 3475 3707 3943 4081 4361 4603 4888 5200
Size: 216 232 236 138 280 242 285 312
Cuts at: 5200 5818 6023 6181 7424 7856 8209 8871 420
Size: 618 205 158 1243 432 353 662 1148
Fragments arranged by size:
1243 1148 1054 663 662 618 513 432
353 312 285 280 242 236 234 232
216 205 203 158 138 93 41 38
EcoNI CCTnn'n_nnAGG
Cuts at: 1378 1815 5240 1378
Size: 437 3425 5737
Fragments arranged by size:
5737 3425 437
EcoO109I rG'GnC_Cy
Cuts at: 222 1314 1371 1608 2148 2253 3508 4233 4526
Size: 1092 57 237 540 105 1255 725 293
Cuts at: 4526 4879 5015 5633 6084 6738 222
Size: 353 136 618 451 654 3083
Fragments arranged by size:
3083 1255 1092 725 654 618 540 451
353 293 237 136 105 57
EcoRI G'AATT_C
Cuts at: 6301 6603 6301
Size: 302 9297
EcoRV GAT'ATC
Cuts at: 1161 3438 4206 1161
Size: 2277 768 6554
Fragments arranged by size:
6554 2277 768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4386 7618 4386
Size: 3232 6367
FspI TGC'GCA
Cuts at: 2260 6247 7380 2260
Size: 3987 1133 4479
Fragments arranged by size:
4479 3987 1133
HaeII r_GCGC'y
Cuts at: 969 1239 3246 4499 4743 4875 6325 7033 7281
Size: 270 2007 1253 244 132 1450 708 248
Cuts at: 7281 8511 9282 969
Size: 1230 771 1286
Fragments arranged by size:
2007 1450 1286 1253 1230 771 708 270
248 244 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 772 804 1245 1277 1632 1664 2884
Size: 32 622 32 441 32 355 32 1220
Cuts at: 2884 2916 3118 3150 5713 5745 7441 7471 7473
Size: 32 202 32 2563 32 1696 30 2
Cuts at: 7473 7503 118
Size: 30 2214
Fragments arranged by size:
2563 2214 1696 1220 622 441 355 202
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 173 4278 6483 6638 173
Size: 4105 2205 155 3134
Fragments arranged by size:
4105 3134 2205 155
HindIII A'AGCT_T
Cuts at: 4297 4585 6395 4297
Size: 288 1810 7501
Fragments arranged by size:
7501 1810 288
KasI G'GCGC_C
Cuts at: 4495 7029 7277 4495
Size: 2534 248 6817
Fragments arranged by size:
6817 2534 248
KpnI G_GTAC'C
Cuts at: 586 718 1758 3168 5323 6619 586
Size: 132 1040 1410 2155 1296 3566
Fragments arranged by size:
3566 2155 1410 1296 1040 132
MluI A'CGCG_T
Cuts at: 9032 9032
Size: 9599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 104 2303 2356 5519 6045 8358 8542 104
Size: 2199 53 3163 526 2313 184 1161
Fragments arranged by size:
3163 2313 2199 1161 526 184 53
MscI TGG'CCA
Cuts at: 1328 1999 2649 2989 3631 5355 7360 1328
Size: 671 650 340 642 1724 2005 3567
Fragments arranged by size:
3567 2005 1724 671 650 642 340
MslI CAynn'nnrTG
Cuts at: 401 1621 1963 2830 2989 3404 4414 5164 6148
Size: 1220 342 867 159 415 1010 750 984
Cuts at: 6148 6337 6559 7588 7715 401
Size: 189 222 1029 127 2285
Fragments arranged by size:
2285 1220 1029 1010 984 867 750 415
342 222 189 159 127
MspA1I CmG'CkG
Cuts at: 1192 1741 2362 2420 2463 2641 2973 3546 3803
Size: 549 621 58 43 178 332 573 257
Cuts at: 3803 3964 4000 4050 4177 4390 5512 6284 6710
Size: 161 36 50 127 213 1122 772 426
Cuts at: 6710 7024 7384 8168 8413 1192
Size: 314 360 784 245 2378
Fragments arranged by size:
2378 1122 784 772 621 573 549 426
360 332 314 257 245 213 178 161
127 58 50 43 36
NaeI GCC'GGC
Cuts at: 3786 4126 5083 7780 3786
Size: 340 957 2697 5605
Fragments arranged by size:
5605 2697 957 340
NarI GG'CG_CC
Cuts at: 4496 7030 7278 4496
Size: 2534 248 6817
Fragments arranged by size:
6817 2534 248
NcoI C'CATG_G
Cuts at: 107 990 2960 2990 4412 5867 107
Size: 883 1970 30 1422 1455 3839
Fragments arranged by size:
3839 1970 1455 1422 883 30
NdeI CA'TA_TG
Cuts at: 4753 4753
Size: 9599
NgoMIV G'CCGG_C
Cuts at: 3784 4124 5081 7778 3784
Size: 340 957 2697 5605
Fragments arranged by size:
5605 2697 957 340
NheI G'CTAG_C
Cuts at: 2043 4068 2043
Size: 2025 7574
NspI r_CATG'y
Cuts at: 1693 2458 2874 3382 3481 4276 5211 6252 6552
Size: 765 416 508 99 795 935 1041 300
Cuts at: 6552 6652 7152 8755 1693
Size: 100 500 1603 2537
Fragments arranged by size:
2537 1603 1041 935 795 765 508 500
416 300 100 99
PasI CC'CwG_GG
Cuts at: 719 1201 1249 1437 1785 5032 5443 719
Size: 482 48 188 348 3247 411 4875
Fragments arranged by size:
4875 3247 482 411 348 188 48
PciI A'CATG_T
Cuts at: 2870 8751 2870
Size: 5881 3718
PflMI CCAn_nnn'nTGG
Cuts at: 398 879 1443 3159 4482 4641 4926 4992 5628
Size: 481 564 1716 1323 159 285 66 636
Cuts at: 5628 398
Size: 4369
Fragments arranged by size:
4369 1716 1323 636 564 481 285 159
66
PfoI T'CCnGG_A
Cuts at: 3305 4058 3305
Size: 753 8846
PmeI GTTT'AAAC
Cuts at: 6598 6598
Size: 9599
PmlI CAC'GTG
Cuts at: 4761 4761
Size: 9599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8045 8077 8045
Size: 32 9567
PpuMI rG'GwC_Cy
Cuts at: 1608 2148 3508 5633 1608
Size: 540 1360 2125 5574
Fragments arranged by size:
5574 2125 1360 540
PspXI vC'TCGA_Gb
Cuts at: 3130 3130
Size: 9599
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6535 6567 6535
Size: 32 9567
PstI C_TGCA'G
Cuts at: 434 3524 4575 5108 6646 7331 434
Size: 3090 1051 533 1538 685 2702
Fragments arranged by size:
3090 2702 1538 1051 685 533
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9599
PvuII CAG'CTG
Cuts at: 1192 2362 2463 2641 2973 3546 3964 4000 5512
Size: 1170 101 178 332 573 418 36 1512
Cuts at: 5512 7024 7384 1192
Size: 1512 360 3407
Fragments arranged by size:
3407 1512 1512 1170 573 418 360 332
178 101 36
RsrII CG'GwC_CG
Cuts at: 7794 7794
Size: 9599
SacI G_AGCT'C
Cuts at: 1829 2072 2405 2933 3122 6613 1829
Size: 243 333 528 189 3491 4815
Fragments arranged by size:
4815 3491 528 333 243 189
SacII CC_GC'GG
Cuts at: 6285 6285
Size: 9599
SalI G'TCGA_C
Cuts at: 171 6636 171
Size: 6465 3134
SanDI GG'GwC_CC
Cuts at: 5633 5633
Size: 9599
SapI GCTCTTCn'nnn_
Cuts at: 2191 7622 7832 2191
Size: 5431 210 3958
Fragments arranged by size:
5431 3958 210
SbfI CC_TGCA'GG
Cuts at: 6646 6646
Size: 9599
ScaI AGT'ACT
Cuts at: 3771 9116 3771
Size: 5345 4254
SexAI A'CCwGG_T
Cuts at: 4347 4347
Size: 9599
SfcI C'TryA_G
Cuts at: 33 430 2783 3496 3520 3936 4571 5104 6642
Size: 397 2353 713 24 416 635 533 1538
Cuts at: 6642 7327 8295 8486 33
Size: 685 968 191 1146
Fragments arranged by size:
2353 1538 1146 968 713 685 635 533
416 397 191 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 2005 6274 2005
Size: 4269 5330
SfoI GGC'GCC
Cuts at: 4497 7031 7279 4497
Size: 2534 248 6817
Fragments arranged by size:
6817 2534 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9599
SmaI CCC'GGG
Cuts at: 5327 6439 6621 5327
Size: 1112 182 8305
Fragments arranged by size:
8305 1112 182
SmlI C'TyrA_G
Cuts at: 661 762 2932 3130 3817 6754 7669 8383 8645
Size: 101 2170 198 687 2937 915 714 262
Cuts at: 8645 661
Size: 1615
Fragments arranged by size:
2937 2170 1615 915 714 687 262 198
101
SnaBI TAC'GTA
Cuts at: 1501 1501
Size: 9599
SpeI A'CTAG_T
Cuts at: 67 5398 67
Size: 5331 4268
SphI G_CATG'C
Cuts at: 3481 6252 6652 7152 3481
Size: 2771 400 500 5928
Fragments arranged by size:
5928 2771 500 400
SspI AAT'ATT
Cuts at: 1416 1416
Size: 9599
StuI AGG'CCT
Cuts at: 2242 2242
Size: 9599
StyI C'CwwG_G
Cuts at: 107 990 1574 2730 2960 2990 3533 3626 4148
Size: 883 584 1156 230 30 543 93 522
Cuts at: 4148 4236 4412 4521 4550 4991 5219 5717 5867
Size: 88 176 109 29 441 228 498 150
Cuts at: 5867 6733 7087 107
Size: 866 354 2619
Fragments arranged by size:
2619 1156 883 866 584 543 522 498
441 354 230 228 176 150 109 93
88 30 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 126 307 1130 1657 1777 2707 2953 3598 3742
Size: 181 823 527 120 930 246 645 144
Cuts at: 3742 6094 6142 6217 6270 7182 7315 7774 7983
Size: 2352 48 75 53 912 133 459 209
Cuts at: 7983 126
Size: 1742
Fragments arranged by size:
2352 1742 930 912 823 645 527 459
246 209 181 144 133 120 75 53
48
TatI w'GTAC_w
Cuts at: 3769 6177 6551 9114 3769
Size: 2408 374 2563 4254
Fragments arranged by size:
4254 2563 2408 374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1676 1708 2001 2033 2705 2737 3689 3721 4021
Size: 32 293 32 672 32 952 32 300
Cuts at: 4021 4053 4921 4953 4987 5012 5019 5044 5173
Size: 32 868 32 34 25 7 25 129
Cuts at: 5173 5205 5873 5905 9116 9148 1676
Size: 32 668 32 3211 32 2127
Fragments arranged by size:
3211 2127 952 868 672 668 300 293
129 34 32 32 32 32 32 32
32 32 32 25 25 7
Tth111I GACn'n_nGTC
Cuts at: 1453 5977 7396 1453
Size: 4524 1419 3656
Fragments arranged by size:
4524 3656 1419
XbaI T'CTAG_A
Cuts at: 6630 6630
Size: 9599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2046 4224 5874 6450 7094 2046
Size: 2178 1650 576 644 4551
Fragments arranged by size:
4551 2178 1650 644 576
XhoI C'TCGA_G
Cuts at: 2932 3130 2932
Size: 198 9401
XmaI C'CCGG_G
Cuts at: 5325 6437 6619 5325
Size: 1112 182 8305
Fragments arranged by size:
8305 1112 182
XmnI GAAnn'nnTTC
Cuts at: 3061 3061
Size: 9599
ZraI GAC'GTC
Cuts at: 5976 5976
Size: 9599
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AgeI AloI ApaI AscI BbsI BfrBI BspEI BssHII
BstZ17I Bsu36I CspCI FseI FspAI HpaI MfeI NotI
NruI NsiI PacI PshAI PsiI PspOMI SgrAI SrfI
SwaI