Restriction map for
pF1KB3243
(Circular) (Six-base) MAPSORT of: pF1KB3243.seq Check: 3624 from: 1 to: 5918
pF1KB3243 5918 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 697 697
Size: 5918
AccI GT'mk_AC
Cuts at: 2660 2956 2660
Size: 296 5622
Acc65I G'GTAC_C
Cuts at: 2934 2934
Size: 5918
AclI AA'CG_TT
Cuts at: 5758 5758
Size: 5918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 308 461 1154 1216 2295 2675 3743 4175
Size: 10 153 693 62 1079 380 1068 432
Cuts at: 4175 4528 5190 298
Size: 353 662 1026
Fragments arranged by size:
1079 1068 1026 693 662 432 380 353
153 62 10
AflIII A'CryG_T
Cuts at: 3270 5070 5351 3270
Size: 1800 281 3837
Fragments arranged by size:
3837 1800 281
AleI CACnn'nnGTG
Cuts at: 1999 1999
Size: 5918
AlwNI CAG_nnn'CTG
Cuts at: 343 1986 2401 3223 4661 343
Size: 1643 415 822 1438 1600
Fragments arranged by size:
1643 1600 1438 822 415
ApaI G_GGCC'C
Cuts at: 645 938 1354 645
Size: 293 416 5209
Fragments arranged by size:
5209 416 293
ApaLI G'TGCA_C
Cuts at: 1934 2460 4756 1934
Size: 526 2296 3096
Fragments arranged by size:
3096 2296 526
ApoI r'AATT_y
Cuts at: 426 997 2354 2922 5271 5527 426
Size: 571 1357 568 2349 256 817
Fragments arranged by size:
2349 1357 817 571 568 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5918
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5918
AvaI C'yCGr_G
Cuts at: 466 1112 2938 466
Size: 646 1826 3446
Fragments arranged by size:
3446 1826 646
BamHI G'GATC_C
Cuts at: 2943 2943
Size: 5918
BanI G'GyrC_C
Cuts at: 111 588 1300 1545 2488 2934 3348 3596 3631
Size: 477 712 245 943 446 414 248 35
Cuts at: 3631 111
Size: 2398
Fragments arranged by size:
2398 943 712 477 446 414 248 245
35
BanII G_rGCy'C
Cuts at: 645 938 1354 1904 2217 2932 3962 645
Size: 293 416 550 313 715 1030 2601
Fragments arranged by size:
2601 1030 715 550 416 313 293
BbeI G_GCGC'C
Cuts at: 115 3352 3600 115
Size: 3237 248 2433
Fragments arranged by size:
3237 2433 248
BbsI GAAGACnn'nnnn_
Cuts at: 383 1853 383
Size: 1470 4448
BciVI GTATCCnnnnn_n'
Cuts at: 1704 2385 3816 4872 5918 1704
Size: 681 1431 1056 1046 1704
Fragments arranged by size:
1704 1431 1056 1046 681
BclI T'GATC_A
Cuts at: 449 3438 449
Size: 2989 2929
BfrBI ATG'CAT
Cuts at: 651 2380 651
Size: 1729 4189
BglII A'GATC_T
Cuts at: 140 1917 2603 3433 5470 140
Size: 1777 686 830 2037 588
Fragments arranged by size:
2037 1777 830 686 588
BlpI GC'TnA_GC
Cuts at: 3030 3030
Size: 5918
Bme1580I G_kGCm'C
Cuts at: 645 938 1305 1354 1938 1996 2464 3543 3636
Size: 293 367 49 584 58 468 1079 93
Cuts at: 3636 4760 645
Size: 1124 1803
Fragments arranged by size:
1803 1124 1079 584 468 367 293 93
58 49
BmrI ACTGGGnnnn_n'
Cuts at: 1779 1873 2478 2563 3130 3211 3546 5690 1779
Size: 94 605 85 567 81 335 2144 2007
Fragments arranged by size:
2144 2007 605 567 335 94 85 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 541 4285 541
Size: 3744 2174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1982 2008 2311 1012
Size: 970 26 303 4619
Fragments arranged by size:
4619 970 303 26
Bpu10I CC'TnA_GC
Cuts at: 2493 2504 2493
Size: 11 5907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 2059 2203 2545 3094 3973 4687 4985 1027
Size: 1032 144 342 549 879 714 298 1960
Fragments arranged by size:
1960 1032 879 714 549 342 298 144
BsaI GGTCTCn'nnnn_
Cuts at: 5622 5622
Size: 5918
BsaAI yAC'GTr
Cuts at: 1221 2187 3271 1221
Size: 966 1084 3868
Fragments arranged by size:
3868 1084 966
BsaBI GATnn'nnATC
Cuts at: 6 254 298 3432 5469 6
Size: 248 44 3134 2037 455
Fragments arranged by size:
3134 2037 455 248 44
BsaHI Gr'CG_yC
Cuts at: 112 3349 3597 4299 5795 112
Size: 3237 248 702 1496 235
Fragments arranged by size:
3237 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 3107 3628 4306 4717 4864 5138 3107
Size: 521 678 411 147 274 3887
Fragments arranged by size:
3887 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1889 1919 1889
Size: 30 5888
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 733 733
Size: 5918
BseYI C'CCAG_C
Cuts at: 502 2533 3343 4101 4766 5174 502
Size: 2031 810 758 665 408 1246
Fragments arranged by size:
2031 1246 810 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 424
Size: 5918
BsiEI CG_ry'CG
Cuts at: 104 3460 3506 4736 104
Size: 3356 46 1230 1286
Fragments arranged by size:
3356 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 967 1938 2464 2833 2932 3710 3900 4760 967
Size: 971 526 369 99 778 190 860 2125
Fragments arranged by size:
2125 971 860 778 526 369 190 99
BsmI GAATG_Cn'
Cuts at: 2380 2382 2380
Size: 2 5916
Bsp1286I G_dGCh'C
Cuts at: 645 938 967 1305 1354 1904 1938 1996 2217
Size: 293 29 338 49 550 34 58 221
Cuts at: 2217 2464 2833 2932 3543 3636 3710 3900 3962
Size: 247 369 99 611 93 74 190 62
Cuts at: 3962 4760 645
Size: 798 1803
Fragments arranged by size:
1803 798 611 550 369 338 293 247
221 190 99 93 74 62 58 49
34 29
BspHI T'CATG_A
Cuts at: 1503 4350 1503
Size: 2847 3071
BspMI ACCTGCnnnn'nnnn_
Cuts at: 697 2968 3182 3484 3865 697
Size: 2271 214 302 381 2750
Fragments arranged by size:
2750 2271 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4210 4264 5748 5913 50
Size: 4160 54 1484 165 55
Fragments arranged by size:
4160 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 956 2054 2591 3830 956
Size: 1098 537 1239 3044
Fragments arranged by size:
3044 1239 1098 537
BsrFI r'CCGG_y
Cuts at: 1202 2162 3916 4097 1202
Size: 960 1754 181 3023
Fragments arranged by size:
3023 1754 960 181
BsrGI T'GTAC_A
Cuts at: 387 387
Size: 5918
BssSI C'ACGA_G
Cuts at: 4189 4897 4189
Size: 708 5210
BstAPI GCAn_nnn'nTGC
Cuts at: 657 3295 657
Size: 2638 3280
BstBI TT'CG_AA
Cuts at: 4279 4279
Size: 5918
BstEII G'GTnAC_C
Cuts at: 520 520
Size: 5918
BstXI CCAn_nnnn'nTGG
Cuts at: 1013 2697 1013
Size: 1684 4234
BstYI r'GATC_y
Cuts at: 1 140 1917 2603 2943 3410 3433 3768 4014
Size: 139 1777 686 340 467 23 335 246
Cuts at: 4014 4320 4332 4418 5470 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1777 1052 686 467 449 340 335 306
246 139 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 554 1729 554
Size: 1175 4743
BtgI C'CryG_G
Cuts at: 107 2240 107
Size: 2133 3785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2016 3868 4049 4212 2016
Size: 1852 181 163 3722
Fragments arranged by size:
3722 1852 181 163
BtsI GCAGTG_nn'
Cuts at: 1454 2246 3161 1454
Size: 792 915 4211
Fragments arranged by size:
4211 915 792
DraI TTT'AAA
Cuts at: 2917 2917
Size: 5918
DraIII CAC_nnn'GTG
Cuts at: 2190 2190
Size: 5918
DrdI GACnn_nn'nnGTC
Cuts at: 3624 4968 3624
Size: 1344 4574
EaeI y'GGCC_r
Cuts at: 1481 1802 2369 3503 3677 4068 4095 5440 1481
Size: 321 567 1134 174 391 27 1345 1959
Fragments arranged by size:
1959 1345 1134 567 391 321 174 27
EagI C'GGCC_G
Cuts at: 3503 3503
Size: 5918
EarI CTCTTCn'nnn_
Cuts at: 284 1130 1409 1640 2267 3941 4151 284
Size: 846 279 231 627 1674 210 2051
Fragments arranged by size:
2051 1674 846 627 279 231 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4126 4868 5014 5728 4126
Size: 742 146 714 4316
Fragments arranged by size:
4316 742 714 146
EcoICRI GAG'CTC
Cuts at: 2930 2930
Size: 5918
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 308 461 1012 1154 1216 1982 2008 2295
Size: 10 153 551 142 62 766 26 287
Cuts at: 2295 2311 2675 3743 4175 4528 5190 298
Size: 16 364 1068 432 353 662 1026
Fragments arranged by size:
1068 1026 766 662 551 432 364 353
287 153 142 62 26 16 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2503 2503
Size: 5918
EcoO109I rG'GnC_Cy
Cuts at: 641 642 934 1350 1351 3057 641
Size: 1 292 416 1 1706 3502
Fragments arranged by size:
3502 1706 416 292 1 1
EcoRI G'AATT_C
Cuts at: 426 2354 2922 426
Size: 1928 568 3422
Fragments arranged by size:
3422 1928 568
EcoRV GAT'ATC
Cuts at: 1794 1794
Size: 5918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 579 1548 2508 2691 3937 579
Size: 969 960 183 1246 2560
Fragments arranged by size:
2560 1246 969 960 183
FspI TGC'GCA
Cuts at: 3699 3699
Size: 5918
HaeII r_GCGC'y
Cuts at: 115 1379 3352 3600 4830 5601 115
Size: 1264 1973 248 1230 771 432
Fragments arranged by size:
1973 1264 1230 771 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 693 725 1889 1921 2383 2415 3760 3790 3792
Size: 32 1164 32 462 32 1345 30 2
Cuts at: 3792 3822 693
Size: 30 2789
Fragments arranged by size:
2789 1345 1164 462 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2957 2957
Size: 5918
HindIII A'AGCT_T
Cuts at: 2077 2077
Size: 5918
KasI G'GCGC_C
Cuts at: 111 3348 3596 111
Size: 3237 248 2433
Fragments arranged by size:
3237 2433 248
KpnI G_GTAC'C
Cuts at: 2938 2938
Size: 5918
MluI A'CGCG_T
Cuts at: 5351 5351
Size: 5918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1267 1688 1780 4677 4861 1267
Size: 421 92 2897 184 2324
Fragments arranged by size:
2897 2324 421 184 92
MscI TGG'CCA
Cuts at: 1804 3679 1804
Size: 1875 4043
MslI CAynn'nnrTG
Cuts at: 713 742 1408 1999 2134 2695 3907 4034 713
Size: 29 666 591 135 561 1212 127 2597
Fragments arranged by size:
2597 1212 666 591 561 135 127 29
MspA1I CmG'CkG
Cuts at: 3029 3343 3703 4487 4732 3029
Size: 314 360 784 245 4215
Fragments arranged by size:
4215 784 360 314 245
NaeI GCC'GGC
Cuts at: 4099 4099
Size: 5918
NarI GG'CG_CC
Cuts at: 112 3349 3597 112
Size: 3237 248 2433
Fragments arranged by size:
3237 2433 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5918
NgoMIV G'CCGG_C
Cuts at: 4097 4097
Size: 5918
NruI TCG'CGA
Cuts at: 2774 2774
Size: 5918
NsiI A_TGCA'T
Cuts at: 653 2382 653
Size: 1729 4189
NspI r_CATG'y
Cuts at: 610 2971 3471 5074 610
Size: 2361 500 1603 1454
Fragments arranged by size:
2361 1603 1454 500
PasI CC'CwG_GG
Cuts at: 1251 1251
Size: 5918
PciI A'CATG_T
Cuts at: 5070 5070
Size: 5918
PflMI CCAn_nnn'nTGG
Cuts at: 1694 2190 1694
Size: 496 5422
PfoI T'CCnGG_A
Cuts at: 989 989
Size: 5918
PmeI GTTT'AAAC
Cuts at: 2917 2917
Size: 5918
PmlI CAC'GTG
Cuts at: 2187 2187
Size: 5918
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 4364
Size: 32 5886
PspOMI G'GGCC_C
Cuts at: 641 934 1350 641
Size: 293 416 5209
Fragments arranged by size:
5209 416 293
PstI C_TGCA'G
Cuts at: 1383 1461 2965 3650 1383
Size: 78 1504 685 3651
Fragments arranged by size:
3651 1504 685 78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5918
PvuII CAG'CTG
Cuts at: 3343 3703 3343
Size: 360 5558
RsrII CG'GwC_CG
Cuts at: 4113 4113
Size: 5918
SacI G_AGCT'C
Cuts at: 2932 2932
Size: 5918
SalI G'TCGA_C
Cuts at: 2955 2955
Size: 5918
SapI GCTCTTCn'nnn_
Cuts at: 3941 4151 3941
Size: 210 5708
SbfI CC_TGCA'GG
Cuts at: 2965 2965
Size: 5918
ScaI AGT'ACT
Cuts at: 5435 5435
Size: 5918
SexAI A'CCwGG_T
Cuts at: 201 201
Size: 5918
SfcI C'TryA_G
Cuts at: 33 1379 1457 2750 2961 3646 4614 4805 33
Size: 1346 78 1293 211 685 968 191 1146
Fragments arranged by size:
1346 1293 1146 968 685 211 191 78
SfoI GGC'GCC
Cuts at: 113 3350 3598 113
Size: 3237 248 2433
Fragments arranged by size:
3237 2433 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5918
SmaI CCC'GGG
Cuts at: 2940 2940
Size: 5918
SmlI C'TyrA_G
Cuts at: 1042 1112 2074 2218 2560 3073 3988 4702 4964
Size: 70 962 144 342 513 915 714 262
Cuts at: 4964 1042
Size: 1996
Fragments arranged by size:
1996 962 915 714 513 342 262 144
70
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5918
SphI G_CATG'C
Cuts at: 610 2971 3471 610
Size: 2361 500 3057
Fragments arranged by size:
3057 2361 500
SspI AAT'ATT
Cuts at: 227 227
Size: 5918
StyI C'CwwG_G
Cuts at: 107 1296 2267 3052 3406 107
Size: 1189 971 785 354 2619
Fragments arranged by size:
2619 1189 971 785 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 856 2547 3501 3634 4093 4302 856
Size: 1691 954 133 459 209 2472
Fragments arranged by size:
2472 1691 954 459 209 133
TatI w'GTAC_w
Cuts at: 356 387 5433 356
Size: 31 5046 841
Fragments arranged by size:
5046 841 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 903 935 5435 5467 903
Size: 32 4500 32 1354
Fragments arranged by size:
4500 1354 32 32
Tth111I GACn'n_nGTC
Cuts at: 3715 3715
Size: 5918
XbaI T'CTAG_A
Cuts at: 1842 2949 1842
Size: 1107 4811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 987 1104 1812 3413 987
Size: 117 708 1601 3492
Fragments arranged by size:
3492 1601 708 117
XhoI C'TCGA_G
Cuts at: 1112 1112
Size: 5918
XmaI C'CCGG_G
Cuts at: 2938 2938
Size: 5918
XmnI GAAnn'nnTTC
Cuts at: 2380 2630 2380
Size: 250 5668
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsiWI
BsmBI BspEI BssHII BstZ17I ClaI CspCI FseI FspAI
HpaI MfeI NdeI NheI NotI PacI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI ZraI