Restriction map for
pF1KB3238
(Circular) (Six-base) MAPSORT of: pF1KB3238.seq Check: 6043 from: 1 to: 5309
pF1KB3238 5309 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 2347 2347
Size: 5309
Acc65I G'GTAC_C
Cuts at: 2325 2325
Size: 5309
AclI AA'CG_TT
Cuts at: 5149 5149
Size: 5309
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1037 1818 1874 3134 3566 3919 4581 1037
Size: 781 56 1260 432 353 662 1765
Fragments arranged by size:
1765 1260 781 662 432 353 56
AfeI AGC'GCT
Cuts at: 2068 2068
Size: 5309
AflIII A'CryG_T
Cuts at: 1406 2661 4461 4742 1406
Size: 1255 1800 281 1973
Fragments arranged by size:
1973 1800 1255 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1172 1204 1172
Size: 32 5277
AlwNI CAG_nnn'CTG
Cuts at: 1587 2257 2614 4052 1587
Size: 670 357 1438 2844
Fragments arranged by size:
2844 1438 670 357
ApaLI G'TGCA_C
Cuts at: 343 4147 343
Size: 3804 1505
ApoI r'AATT_y
Cuts at: 776 1763 2313 4662 4918 776
Size: 987 550 2349 256 1167
Fragments arranged by size:
2349 1167 987 550 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5309
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5309
AvaI C'yCGr_G
Cuts at: 1610 2329 1610
Size: 719 4590
BamHI G'GATC_C
Cuts at: 2334 2334
Size: 5309
BanI G'GyrC_C
Cuts at: 2325 2739 2987 3022 2325
Size: 414 248 35 4612
Fragments arranged by size:
4612 414 248 35
BanII G_rGCy'C
Cuts at: 1830 2323 3353 1830
Size: 493 1030 3786
Fragments arranged by size:
3786 1030 493
BbeI G_GCGC'C
Cuts at: 2743 2991 2743
Size: 248 5061
BbsI GAAGACnn'nnnn_
Cuts at: 807 1397 1519 1535 807
Size: 590 122 16 4581
Fragments arranged by size:
4581 590 122 16
BbvCI CC'TCA_GC
Cuts at: 520 1667 2201 520
Size: 1147 534 3628
Fragments arranged by size:
3628 1147 534
BciVI GTATCCnnnnn_n'
Cuts at: 1974 3207 4263 5309 1974
Size: 1233 1056 1046 1974
Fragments arranged by size:
1974 1233 1056 1046
BclI T'GATC_A
Cuts at: 2162 2829 2162
Size: 667 4642
BglII A'GATC_T
Cuts at: 2824 4861 2824
Size: 2037 3272
BlpI GC'TnA_GC
Cuts at: 2421 2421
Size: 5309
Bme1580I G_kGCm'C
Cuts at: 347 2934 3027 4151 347
Size: 2587 93 1124 1505
Fragments arranged by size:
2587 1505 1124 93
BmgBI CAC'GTC
Cuts at: 153 153
Size: 5309
BmrI ACTGGGnnnn_n'
Cuts at: 2521 2602 2937 5081 2521
Size: 81 335 2144 2749
Fragments arranged by size:
2749 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2196 3676 2196
Size: 1480 3829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1827 400
Size: 1427 3882
Bpu10I CC'TnA_GC
Cuts at: 520 660 1667 1757 2201 520
Size: 140 1007 90 444 3628
Fragments arranged by size:
3628 1007 444 140 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1301 2485 3364 4078 4376 742
Size: 559 1184 879 714 298 1675
Fragments arranged by size:
1675 1184 879 714 559 298
BsaI GGTCTCn'nnnn_
Cuts at: 5013 5013
Size: 5309
BsaAI yAC'GTr
Cuts at: 2662 2662
Size: 5309
BsaBI GATnn'nnATC
Cuts at: 6 326 2823 4860 6
Size: 320 2497 2037 455
Fragments arranged by size:
2497 2037 455 320
BsaHI Gr'CG_yC
Cuts at: 2740 2988 3690 5186 2740
Size: 248 702 1496 2863
Fragments arranged by size:
2863 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2498 3019 3697 4108 4255 4529 2498
Size: 521 678 411 147 274 3278
Fragments arranged by size:
3278 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1105 1135 1105
Size: 30 5279
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 656 656
Size: 5309
BseYI C'CCAG_C
Cuts at: 2734 3492 4157 4565 2734
Size: 758 665 408 3478
Fragments arranged by size:
3478 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 1576 1393
Size: 183 5126
BsiEI CG_ry'CG
Cuts at: 104 118 2851 2897 4127 104
Size: 14 2733 46 1230 1286
Fragments arranged by size:
2733 1286 1230 46 14
BsiHKAI G_wGCw'C
Cuts at: 347 2323 3101 3291 4151 347
Size: 1976 778 190 860 1505
Fragments arranged by size:
1976 1505 860 778 190
BsmI GAATG_Cn'
Cuts at: 834 1467 834
Size: 633 4676
BsmBI CGTCTCn'nnnn_
Cuts at: 1845 1845
Size: 5309
Bsp1286I G_dGCh'C
Cuts at: 347 1830 2323 2934 3027 3101 3291 3353 4151
Size: 1483 493 611 93 74 190 62 798
Cuts at: 4151 347
Size: 1505
Fragments arranged by size:
1505 1483 798 611 493 190 93 74
62
BspHI T'CATG_A
Cuts at: 3741 3741
Size: 5309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2359 2573 2875 3256 2359
Size: 214 302 381 4412
Fragments arranged by size:
4412 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3601 3655 5139 5304 50
Size: 3551 54 1484 165 55
Fragments arranged by size:
3551 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3221 3221
Size: 5309
BsrFI r'CCGG_y
Cuts at: 3307 3488 3307
Size: 181 5128
BsrGI T'GTAC_A
Cuts at: 231 231
Size: 5309
BssSI C'ACGA_G
Cuts at: 3580 4288 3580
Size: 708 4601
BstAPI GCAn_nnn'nTGC
Cuts at: 2018 2686 2018
Size: 668 4641
BstBI TT'CG_AA
Cuts at: 3670 3670
Size: 5309
BstXI CCAn_nnnn'nTGG
Cuts at: 1494 1786 1494
Size: 292 5017
BstYI r'GATC_y
Cuts at: 1 2334 2801 2824 3159 3405 3711 3723 3809
Size: 2333 467 23 335 246 306 12 86
Cuts at: 3809 4861 1
Size: 1052 449
Fragments arranged by size:
2333 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 669 107
Size: 562 4747
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3259 3440 3603 3259
Size: 181 163 4965
Fragments arranged by size:
4965 181 163
BtsI GCAGTG_nn'
Cuts at: 1866 2552 1866
Size: 686 4623
ClaI AT'CG_AT
Cuts at: 904 904
Size: 5309
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2223 2258 2223
Size: 35 5274
DraI TTT'AAA
Cuts at: 1025 2308 1025
Size: 1283 4026
DrdI GACnn_nn'nnGTC
Cuts at: 3015 4359 3015
Size: 1344 3965
EaeI y'GGCC_r
Cuts at: 666 1277 2894 3068 3459 3486 4831 666
Size: 611 1617 174 391 27 1345 1144
Fragments arranged by size:
1617 1345 1144 611 391 174 27
EagI C'GGCC_G
Cuts at: 2894 2894
Size: 5309
EarI CTCTTCn'nnn_
Cuts at: 487 2002 2054 3332 3542 487
Size: 1515 52 1278 210 2254
Fragments arranged by size:
2254 1515 1278 210 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3517 4259 4405 5119 3517
Size: 742 146 714 3707
Fragments arranged by size:
3707 742 714 146
EcoICRI GAG'CTC
Cuts at: 2321 2321
Size: 5309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1037 1818 1827 1874 3134 3566 3919 4581
Size: 637 781 9 47 1260 432 353 662
Cuts at: 4581 400
Size: 1128
Fragments arranged by size:
1260 1128 781 662 637 432 353 47
9
EcoO109I rG'GnC_Cy
Cuts at: 2448 2448
Size: 5309
EcoRI G'AATT_C
Cuts at: 2313 2313
Size: 5309
EcoRV GAT'ATC
Cuts at: 746 746
Size: 5309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 736 1947 3328 736
Size: 1211 1381 2717
Fragments arranged by size:
2717 1381 1211
FspI TGC'GCA
Cuts at: 3090 3090
Size: 5309
HaeII r_GCGC'y
Cuts at: 2070 2743 2991 4221 4992 2070
Size: 673 248 1230 771 2387
Fragments arranged by size:
2387 1230 771 673 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3151 3181 3183 3213 3151
Size: 30 2 30 5247
Fragments arranged by size:
5247 30 30 2
HincII GTy'rAC
Cuts at: 2348 2348
Size: 5309
KasI G'GCGC_C
Cuts at: 2739 2987 2739
Size: 248 5061
KpnI G_GTAC'C
Cuts at: 2329 2329
Size: 5309
MluI A'CGCG_T
Cuts at: 4742 4742
Size: 5309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 426 749 1564 1854 4068 4252 426
Size: 323 815 290 2214 184 1483
Fragments arranged by size:
2214 1483 815 323 290 184
MscI TGG'CCA
Cuts at: 668 1279 3070 668
Size: 611 1791 2907
Fragments arranged by size:
2907 1791 611
MslI CAynn'nnrTG
Cuts at: 340 454 1352 1784 3298 3425 340
Size: 114 898 432 1514 127 2224
Fragments arranged by size:
2224 1514 898 432 127 114
MspA1I CmG'CkG
Cuts at: 133 546 1225 1377 1666 2200 2257 2420 2734
Size: 413 679 152 289 534 57 163 314
Cuts at: 2734 3094 3878 4123 133
Size: 360 784 245 1319
Fragments arranged by size:
1319 784 679 534 413 360 314 289
245 163 152 57
NaeI GCC'GGC
Cuts at: 3490 3490
Size: 5309
NarI GG'CG_CC
Cuts at: 2740 2988 2740
Size: 248 5061
NcoI C'CATG_G
Cuts at: 107 669 107
Size: 562 4747
NgoMIV G'CCGG_C
Cuts at: 3488 3488
Size: 5309
NspI r_CATG'y
Cuts at: 1410 2362 2862 4465 1410
Size: 952 500 1603 2254
Fragments arranged by size:
2254 1603 952 500
PciI A'CATG_T
Cuts at: 1406 4461 1406
Size: 3055 2254
PflMI CCAn_nnn'nTGG
Cuts at: 845 845
Size: 5309
PfoI T'CCnGG_A
Cuts at: 428 2033 2144 428
Size: 1605 111 3593
Fragments arranged by size:
3593 1605 111
PmeI GTTT'AAAC
Cuts at: 2308 2308
Size: 5309
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 5277
PsiI TTA'TAA
Cuts at: 556 556
Size: 5309
PstI C_TGCA'G
Cuts at: 1230 2356 3041 1230
Size: 1126 685 3498
Fragments arranged by size:
3498 1126 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5309
PvuII CAG'CTG
Cuts at: 133 546 1225 1377 2200 2257 2734 3094 133
Size: 413 679 152 823 57 477 360 2348
Fragments arranged by size:
2348 823 679 477 413 360 152 57
RsrII CG'GwC_CG
Cuts at: 3504 3504
Size: 5309
SacI G_AGCT'C
Cuts at: 2323 2323
Size: 5309
SalI G'TCGA_C
Cuts at: 2346 2346
Size: 5309
SapI GCTCTTCn'nnn_
Cuts at: 2002 2054 3332 3542 2002
Size: 52 1278 210 3769
Fragments arranged by size:
3769 1278 210 52
SbfI CC_TGCA'GG
Cuts at: 2356 2356
Size: 5309
ScaI AGT'ACT
Cuts at: 4826 4826
Size: 5309
SexAI A'CCwGG_T
Cuts at: 405 405
Size: 5309
SfcI C'TryA_G
Cuts at: 33 910 1226 1793 2352 3037 4005 4196 33
Size: 877 316 567 559 685 968 191 1146
Fragments arranged by size:
1146 968 877 685 567 559 316 191
SfoI GGC'GCC
Cuts at: 2741 2989 2741
Size: 248 5061
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5309
SmaI CCC'GGG
Cuts at: 1612 2331 1612
Size: 719 4590
SmlI C'TyrA_G
Cuts at: 721 1316 2464 3379 4093 4355 721
Size: 595 1148 915 714 262 1675
Fragments arranged by size:
1675 1148 915 714 595 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5309
SphI G_CATG'C
Cuts at: 2362 2862 2362
Size: 500 4809
SspI AAT'ATT
Cuts at: 509 509
Size: 5309
StyI C'CwwG_G
Cuts at: 107 669 2443 2797 107
Size: 562 1774 354 2619
Fragments arranged by size:
2619 1774 562 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2892 3025 3484 3693 2892
Size: 133 459 209 4508
Fragments arranged by size:
4508 459 209 133
TatI w'GTAC_w
Cuts at: 231 4824 231
Size: 4593 716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 288 320 1105 1137 4826 4858 288
Size: 32 785 32 3689 32 739
Fragments arranged by size:
3689 785 739 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3106 3106
Size: 5309
XbaI T'CTAG_A
Cuts at: 2340 2340
Size: 5309
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2217 2804 2217
Size: 587 4722
XmaI C'CCGG_G
Cuts at: 1610 2329 1610
Size: 719 4590
XmnI GAAnn'nnTTC
Cuts at: 1123 1123
Size: 5309
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsiWI
BspEI BssHII BstEII BstZ17I Bsu36I DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI