Restriction map for
pF1KB3233
(Circular) (Six-base) MAPSORT of: pF1KB3233.seq Check: 3934 from: 1 to: 5453
pF1KB3233 5453 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2491 2491
Size: 5453
Acc65I G'GTAC_C
Cuts at: 2469 2469
Size: 5453
AclI AA'CG_TT
Cuts at: 5293 5293
Size: 5453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1240 1704 2305 2375 3278 3710 4063 4725
Size: 918 464 601 70 903 432 353 662
Cuts at: 4725 322
Size: 1050
Fragments arranged by size:
1050 918 903 662 601 464 432 353
70
AflIII A'CryG_T
Cuts at: 2805 4605 4886 2805
Size: 1800 281 3372
Fragments arranged by size:
3372 1800 281
AgeI A'CCGG_T
Cuts at: 238 238
Size: 5453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1420 1420
Size: 5453
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 852 884 852
Size: 32 5421
AlwNI CAG_nnn'CTG
Cuts at: 1318 1789 2278 2758 4196 1318
Size: 471 489 480 1438 2575
Fragments arranged by size:
2575 1438 489 480 471
ApaLI G'TGCA_C
Cuts at: 4291 4291
Size: 5453
ApoI r'AATT_y
Cuts at: 1037 1204 2127 2457 4806 5062 1037
Size: 167 923 330 2349 256 1428
Fragments arranged by size:
2349 1428 923 330 256 167
AseI AT'TA_AT
Cuts at: 20 20
Size: 5453
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
AvaI C'yCGr_G
Cuts at: 980 2473 980
Size: 1493 3960
BamHI G'GATC_C
Cuts at: 2478 2478
Size: 5453
BanI G'GyrC_C
Cuts at: 241 619 1792 2146 2469 2883 3131 3166 241
Size: 378 1173 354 323 414 248 35 2528
Fragments arranged by size:
2528 1173 414 378 354 323 248 35
BanII G_rGCy'C
Cuts at: 1338 1661 1904 2110 2259 2467 3497 1338
Size: 323 243 206 149 208 1030 3294
Fragments arranged by size:
3294 1030 323 243 208 206 149
BbeI G_GCGC'C
Cuts at: 2150 2887 3135 2150
Size: 737 248 4468
Fragments arranged by size:
4468 737 248
BbsI GAAGACnn'nnnn_
Cuts at: 243 944 2256 243
Size: 701 1312 3440
Fragments arranged by size:
3440 1312 701
BbvCI CC'TCA_GC
Cuts at: 896 896
Size: 5453
BciVI GTATCCnnnnn_n'
Cuts at: 739 3351 4407 5453 739
Size: 2612 1056 1046 739
Fragments arranged by size:
2612 1056 1046 739
BclI T'GATC_A
Cuts at: 2973 2973
Size: 5453
BglI GCCn_nnn'nGGC
Cuts at: 1496 1496
Size: 5453
BglII A'GATC_T
Cuts at: 2968 5005 2968
Size: 2037 3416
BlpI GC'TnA_GC
Cuts at: 2279 2565 2279
Size: 286 5167
Bme1580I G_kGCm'C
Cuts at: 246 369 3078 3171 4295 246
Size: 123 2709 93 1124 1404
Fragments arranged by size:
2709 1404 1124 123 93
BmrI ACTGGGnnnn_n'
Cuts at: 485 795 831 1260 2665 2746 3081 5225 485
Size: 310 36 429 1405 81 335 2144 713
Fragments arranged by size:
2144 1405 713 429 335 310 81 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3820 3820
Size: 5453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1485 2204 2428 508
Size: 977 719 224 3533
Fragments arranged by size:
3533 977 719 224
Bpu10I CC'TnA_GC
Cuts at: 896 896
Size: 5453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2629 3508 4222 4520 2629
Size: 879 714 298 3562
Fragments arranged by size:
3562 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1910 5157 1910
Size: 3247 2206
BsaAI yAC'GTr
Cuts at: 2806 2806
Size: 5453
BsaBI GATnn'nnATC
Cuts at: 6 2967 5004 6
Size: 2961 2037 455
Fragments arranged by size:
2961 2037 455
BsaHI Gr'CG_yC
Cuts at: 1018 2147 2884 3132 3834 5330 1018
Size: 1129 737 248 702 1496 1141
Fragments arranged by size:
1496 1141 1129 737 702 248
BsaWI w'CCGG_w
Cuts at: 238 2642 3163 3841 4252 4399 4673 238
Size: 2404 521 678 411 147 274 1018
Fragments arranged by size:
2404 1018 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 338 368 338
Size: 30 5423
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 565 1912 2059 565
Size: 1347 147 3959
Fragments arranged by size:
3959 1347 147
BseYI C'CCAG_C
Cuts at: 342 1101 1870 2878 3636 4301 4709 342
Size: 759 769 1008 758 665 408 1086
Fragments arranged by size:
1086 1008 769 759 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1740 1024
Size: 716 4737
BsiEI CG_ry'CG
Cuts at: 104 2444 2995 3041 4271 104
Size: 2340 551 46 1230 1286
Fragments arranged by size:
2340 1286 1230 551 46
BsiHKAI G_wGCw'C
Cuts at: 125 1409 1661 2414 2467 3245 3435 4295 125
Size: 1284 252 753 53 778 190 860 1283
Fragments arranged by size:
1284 1283 860 778 753 252 190 53
BsmI GAATG_Cn'
Cuts at: 204 1359 204
Size: 1155 4298
BsmBI CGTCTCn'nnnn_
Cuts at: 880 880
Size: 5453
Bsp1286I G_dGCh'C
Cuts at: 125 246 369 1338 1409 1661 1904 2110 2259
Size: 121 123 969 71 252 243 206 149
Cuts at: 2259 2414 2467 3078 3171 3245 3435 3497 4295
Size: 155 53 611 93 74 190 62 798
Cuts at: 4295 125
Size: 1283
Fragments arranged by size:
1283 969 798 611 252 243 206 190
155 149 123 121 93 74 71 62
53
BspHI T'CATG_A
Cuts at: 1241 3885 1241
Size: 2644 2809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 523 1928 2503 2717 3019 3400 523
Size: 1405 575 214 302 381 2576
Fragments arranged by size:
2576 1405 575 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3745 3799 5283 5448 50
Size: 3695 54 1484 165 55
Fragments arranged by size:
3695 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 158 2007 3365 158
Size: 1849 1358 2246
Fragments arranged by size:
2246 1849 1358
BsrFI r'CCGG_y
Cuts at: 238 3451 3632 238
Size: 3213 181 2059
Fragments arranged by size:
3213 2059 181
BsrGI T'GTAC_A
Cuts at: 1259 1259
Size: 5453
BssSI C'ACGA_G
Cuts at: 3724 4432 3724
Size: 708 4745
BstAPI GCAn_nnn'nTGC
Cuts at: 2830 2830
Size: 5453
BstBI TT'CG_AA
Cuts at: 3814 3814
Size: 5453
BstEII G'GTnAC_C
Cuts at: 1126 1167 1126
Size: 41 5412
BstXI CCAn_nnnn'nTGG
Cuts at: 141 1191 2142 141
Size: 1050 951 3452
Fragments arranged by size:
3452 1050 951
BstYI r'GATC_y
Cuts at: 1 2204 2478 2945 2968 3303 3549 3855 3867
Size: 2203 274 467 23 335 246 306 12
Cuts at: 3867 3953 5005 1
Size: 86 1052 449
Fragments arranged by size:
2203 1052 467 449 335 306 274 246
86 23 12
BtgI C'CryG_G
Cuts at: 864 1190 1853 864
Size: 326 663 4464
Fragments arranged by size:
4464 663 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3403 3584 3747 3403
Size: 181 163 5109
Fragments arranged by size:
5109 181 163
BtsI GCAGTG_nn'
Cuts at: 689 1073 2696 689
Size: 384 1623 3446
Fragments arranged by size:
3446 1623 384
DraI TTT'AAA
Cuts at: 2452 2452
Size: 5453
DraIII CAC_nnn'GTG
Cuts at: 1314 1314
Size: 5453
DrdI GACnn_nn'nnGTC
Cuts at: 3159 4503 3159
Size: 1344 4109
EaeI y'GGCC_r
Cuts at: 1436 1488 2441 3038 3212 3603 3630 4975 1436
Size: 52 953 597 174 391 27 1345 1914
Fragments arranged by size:
1914 1345 953 597 391 174 52 27
EagI C'GGCC_G
Cuts at: 2441 3038 2441
Size: 597 4856
EarI CTCTTCn'nnn_
Cuts at: 113 1528 3476 3686 113
Size: 1415 1948 210 1880
Fragments arranged by size:
1948 1880 1415 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2217 3661 4403 4549 5263 2217
Size: 1444 742 146 714 2407
Fragments arranged by size:
2407 1444 742 714 146
EcoICRI GAG'CTC
Cuts at: 1659 2465 1659
Size: 806 4647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 508 1240 1485 1704 2204 2305 2375 2428
Size: 186 732 245 219 500 101 70 53
Cuts at: 2428 3278 3710 4063 4725 322
Size: 850 432 353 662 1050
Fragments arranged by size:
1050 850 732 662 500 432 353 245
219 186 101 70 53
EcoNI CCTnn'n_nnAGG
Cuts at: 1390 1438 1390
Size: 48 5405
EcoO109I rG'GnC_Cy
Cuts at: 860 1088 1431 1581 1620 2592 860
Size: 228 343 150 39 972 3721
Fragments arranged by size:
3721 972 343 228 150 39
EcoRI G'AATT_C
Cuts at: 1037 2457 1037
Size: 1420 4033
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1554 2076 3472 1554
Size: 522 1396 3535
Fragments arranged by size:
3535 1396 522
FspI TGC'GCA
Cuts at: 3234 3234
Size: 5453
HaeII r_GCGC'y
Cuts at: 2150 2887 3135 4365 5136 2150
Size: 737 248 1230 771 2467
Fragments arranged by size:
2467 1230 771 737 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 215 247 284 316 426 458 3295 3325 3327
Size: 32 37 32 110 32 2837 30 2
Cuts at: 3327 3357 215
Size: 30 2311
Fragments arranged by size:
2837 2311 110 37 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2492 2492
Size: 5453
HindIII A'AGCT_T
Cuts at: 1264 1264
Size: 5453
KasI G'GCGC_C
Cuts at: 2146 2883 3131 2146
Size: 737 248 4468
Fragments arranged by size:
4468 737 248
KpnI G_GTAC'C
Cuts at: 2473 2473
Size: 5453
MfeI C'AATT_G
Cuts at: 599 905 599
Size: 306 5147
MluI A'CGCG_T
Cuts at: 4886 4886
Size: 5453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2292 4212 4396 1721
Size: 571 1920 184 2778
Fragments arranged by size:
2778 1920 571 184
MscI TGG'CCA
Cuts at: 1438 1490 3214 1438
Size: 52 1724 3677
Fragments arranged by size:
3677 1724 52
MslI CAynn'nnrTG
Cuts at: 625 889 2140 3442 3569 625
Size: 264 1251 1302 127 2509
Fragments arranged by size:
2509 1302 1251 264 127
MspA1I CmG'CkG
Cuts at: 342 1789 1870 2564 2878 3238 4022 4267 342
Size: 1447 81 694 314 360 784 245 1528
Fragments arranged by size:
1528 1447 784 694 360 314 245 81
NaeI GCC'GGC
Cuts at: 3634 3634
Size: 5453
NarI GG'CG_CC
Cuts at: 2147 2884 3132 2147
Size: 737 248 4468
Fragments arranged by size:
4468 737 248
NcoI C'CATG_G
Cuts at: 1190 1853 1190
Size: 663 4790
NgoMIV G'CCGG_C
Cuts at: 3632 3632
Size: 5453
NspI r_CATG'y
Cuts at: 1003 2094 2506 3006 4609 1003
Size: 1091 412 500 1603 1847
Fragments arranged by size:
1847 1603 1091 500 412
PasI CC'CwG_GG
Cuts at: 141 141
Size: 5453
PciI A'CATG_T
Cuts at: 4605 4605
Size: 5453
PflMI CCAn_nnn'nTGG
Cuts at: 2274 2274
Size: 5453
PmeI GTTT'AAAC
Cuts at: 2452 2452
Size: 5453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 5421
PpuMI rG'GwC_Cy
Cuts at: 860 1088 860
Size: 228 5225
PstI C_TGCA'G
Cuts at: 1347 1925 2500 3185 1347
Size: 578 575 685 3615
Fragments arranged by size:
3615 685 578 575
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5453
PvuII CAG'CTG
Cuts at: 342 1789 1870 2878 3238 342
Size: 1447 81 1008 360 2557
Fragments arranged by size:
2557 1447 1008 360 81
RsrII CG'GwC_CG
Cuts at: 2429 3648 2429
Size: 1219 4234
SacI G_AGCT'C
Cuts at: 1661 2467 1661
Size: 806 4647
SalI G'TCGA_C
Cuts at: 2490 2490
Size: 5453
SanDI GG'GwC_CC
Cuts at: 1088 1088
Size: 5453
SapI GCTCTTCn'nnn_
Cuts at: 113 1528 3476 3686 113
Size: 1415 1948 210 1880
Fragments arranged by size:
1948 1880 1415 210
SbfI CC_TGCA'GG
Cuts at: 1925 2500 1925
Size: 575 4878
ScaI AGT'ACT
Cuts at: 4970 4970
Size: 5453
SexAI A'CCwGG_T
Cuts at: 419 419
Size: 5453
SfcI C'TryA_G
Cuts at: 33 1343 1688 1921 2496 3181 4149 4340 33
Size: 1310 345 233 575 685 968 191 1146
Fragments arranged by size:
1310 1146 968 685 575 345 233 191
SfoI GGC'GCC
Cuts at: 2148 2885 3133 2148
Size: 737 248 4468
Fragments arranged by size:
4468 737 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
SgrAI Cr'CCGG_yG
Cuts at: 238 238
Size: 5453
SmaI CCC'GGG
Cuts at: 982 2475 982
Size: 1493 3960
SmlI C'TyrA_G
Cuts at: 2608 3523 4237 4499 2608
Size: 915 714 262 3562
Fragments arranged by size:
3562 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5453
SphI G_CATG'C
Cuts at: 2506 3006 2506
Size: 500 4953
StuI AGG'CCT
Cuts at: 278 278
Size: 5453
StyI C'CwwG_G
Cuts at: 189 1184 1190 1439 1491 1853 2150 2318 2587
Size: 995 6 249 52 362 297 168 269
Cuts at: 2587 2941 189
Size: 354 2701
Fragments arranged by size:
2701 995 362 354 297 269 249 168
52 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1045 1816 3036 3169 3628 3837 1045
Size: 771 1220 133 459 209 2661
Fragments arranged by size:
2661 1220 771 459 209 133
TatI w'GTAC_w
Cuts at: 1259 2036 4968 1259
Size: 777 2932 1744
Fragments arranged by size:
2932 1744 777
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 338 370 2314 2346 4970 5002 338
Size: 32 1944 32 2624 32 789
Fragments arranged by size:
2624 1944 789 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3250 3250
Size: 5453
XbaI T'CTAG_A
Cuts at: 2484 2484
Size: 5453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 818 1860 2948 818
Size: 1042 1088 3323
Fragments arranged by size:
3323 1088 1042
XmaI C'CCGG_G
Cuts at: 980 2473 980
Size: 1493 3960
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BspEI
BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI