Restriction map for
pF1KB3223
(Circular) (Six-base) MAPSORT of: pF1KB3223.seq Check: 8045 from: 1 to: 5231
pF1KB3223 5231 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2269 2269
Size: 5231
Acc65I G'GTAC_C
Cuts at: 1222 2247 1222
Size: 1025 4206
AclI AA'CG_TT
Cuts at: 5071 5071
Size: 5231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 536 3056 3488 3841 4503 159
Size: 377 2520 432 353 662 887
Fragments arranged by size:
2520 887 662 432 377 353
AfeI AGC'GCT
Cuts at: 629 629
Size: 5231
AflII C'TTAA_G
Cuts at: 2116 2116
Size: 5231
AflIII A'CryG_T
Cuts at: 884 1944 2583 4383 4664 884
Size: 1060 639 1800 281 1451
Fragments arranged by size:
1800 1451 1060 639 281
AhdI GACnn_n'nnGTC
Cuts at: 1794 1794
Size: 5231
AlwNI CAG_nnn'CTG
Cuts at: 2536 3974 2536
Size: 1438 3793
ApaLI G'TGCA_C
Cuts at: 4069 4069
Size: 5231
ApoI r'AATT_y
Cuts at: 705 860 2235 4584 4840 705
Size: 155 1375 2349 256 1096
Fragments arranged by size:
2349 1375 1096 256 155
AseI AT'TA_AT
Cuts at: 20 1958 20
Size: 1938 3293
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5231
AvaI C'yCGr_G
Cuts at: 2251 2251
Size: 5231
BamHI G'GATC_C
Cuts at: 2256 2256
Size: 5231
BanI G'GyrC_C
Cuts at: 663 1222 2247 2661 2909 2944 663
Size: 559 1025 414 248 35 2950
Fragments arranged by size:
2950 1025 559 414 248 35
BanII G_rGCy'C
Cuts at: 387 535 2245 3275 387
Size: 148 1710 1030 2343
Fragments arranged by size:
2343 1710 1030 148
BbeI G_GCGC'C
Cuts at: 2665 2913 2665
Size: 248 4983
BbsI GAAGACnn'nnnn_
Cuts at: 970 1598 970
Size: 628 4603
BciVI GTATCCnnnnn_n'
Cuts at: 3129 4185 5231 3129
Size: 1056 1046 3129
Fragments arranged by size:
3129 1056 1046
BclI T'GATC_A
Cuts at: 357 2751 357
Size: 2394 2837
BglI GCCn_nnn'nGGC
Cuts at: 2152 2152
Size: 5231
BglII A'GATC_T
Cuts at: 516 2746 4783 516
Size: 2230 2037 964
Fragments arranged by size:
2230 2037 964
BlpI GC'TnA_GC
Cuts at: 157 2343 157
Size: 2186 3045
Bme1580I G_kGCm'C
Cuts at: 2856 2949 4073 2856
Size: 93 1124 4014
Fragments arranged by size:
4014 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 238 2443 2524 2859 5003 238
Size: 2205 81 335 2144 466
Fragments arranged by size:
2205 2144 466 335 81
BmtI G_CTAG'C
Cuts at: 776 776
Size: 5231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 468 956 3598 468
Size: 488 2642 2101
Fragments arranged by size:
2642 2101 488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1265 1265
Size: 5231
Bpu10I CC'TnA_GC
Cuts at: 749 749
Size: 5231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 951 2407 3286 4000 4298 951
Size: 1456 879 714 298 1884
Fragments arranged by size:
1884 1456 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 600 4935 600
Size: 4335 896
BsaAI yAC'GTr
Cuts at: 2584 2584
Size: 5231
BsaBI GATnn'nnATC
Cuts at: 6 2745 4782 6
Size: 2739 2037 455
Fragments arranged by size:
2739 2037 455
BsaHI Gr'CG_yC
Cuts at: 2662 2910 3612 5108 2662
Size: 248 702 1496 2785
Fragments arranged by size:
2785 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2420 2941 3619 4030 4177 4451 2420
Size: 521 678 411 147 274 3200
Fragments arranged by size:
3200 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 284 618 1037 284
Size: 334 419 4478
Fragments arranged by size:
4478 419 334
BseYI C'CCAG_C
Cuts at: 116 534 2656 3414 4079 4487 116
Size: 418 2122 758 665 408 860
Fragments arranged by size:
2122 860 758 665 418 408
BsiEI CG_ry'CG
Cuts at: 104 2773 2819 4049 104
Size: 2669 46 1230 1286
Fragments arranged by size:
2669 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2245 3023 3213 4073 2245
Size: 778 190 860 3403
Fragments arranged by size:
3403 860 778 190
BsmI GAATG_Cn'
Cuts at: 1070 1095 1966 1070
Size: 25 871 4335
Fragments arranged by size:
4335 871 25
Bsp1286I G_dGCh'C
Cuts at: 387 535 2245 2856 2949 3023 3213 3275 4073
Size: 148 1710 611 93 74 190 62 798
Cuts at: 4073 387
Size: 1545
Fragments arranged by size:
1710 1545 798 611 190 148 93 74
62
BspHI T'CATG_A
Cuts at: 413 1767 3663 413
Size: 1354 1896 1981
Fragments arranged by size:
1981 1896 1354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2281 2495 2797 3178 2281
Size: 214 302 381 4334
Fragments arranged by size:
4334 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3523 3577 5061 5226 50
Size: 3473 54 1484 165 55
Fragments arranged by size:
3473 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1346 3143 1346
Size: 1797 3434
BsrFI r'CCGG_y
Cuts at: 3229 3410 3229
Size: 181 5050
BsrGI T'GTAC_A
Cuts at: 464 464
Size: 5231
BssSI C'ACGA_G
Cuts at: 972 1321 3502 4210 972
Size: 349 2181 708 1993
Fragments arranged by size:
2181 1993 708 349
BstAPI GCAn_nnn'nTGC
Cuts at: 2608 2608
Size: 5231
BstBI TT'CG_AA
Cuts at: 3592 3592
Size: 5231
BstXI CCAn_nnnn'nTGG
Cuts at: 2214 2214
Size: 5231
BstYI r'GATC_y
Cuts at: 1 516 2256 2723 2746 3081 3327 3633 3645
Size: 515 1740 467 23 335 246 306 12
Cuts at: 3645 3731 4783 1
Size: 86 1052 449
Fragments arranged by size:
1740 1052 515 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 2048 2048
Size: 5231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 196 985 3181 3362 3525 196
Size: 789 2196 181 163 1902
Fragments arranged by size:
2196 1902 789 181 163
BtsI GCAGTG_nn'
Cuts at: 2170 2474 2170
Size: 304 4927
DraI TTT'AAA
Cuts at: 2230 2230
Size: 5231
DrdI GACnn_nn'nnGTC
Cuts at: 2937 4281 2937
Size: 1344 3887
EaeI y'GGCC_r
Cuts at: 131 1234 1980 2816 2990 3381 3408 4753 131
Size: 1103 746 836 174 391 27 1345 609
Fragments arranged by size:
1345 1103 836 746 609 391 174 27
EagI C'GGCC_G
Cuts at: 2816 2816
Size: 5231
EarI CTCTTCn'nnn_
Cuts at: 1999 3254 3464 1999
Size: 1255 210 3766
Fragments arranged by size:
3766 1255 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1929 3439 4181 4327 5041 1929
Size: 1510 742 146 714 2119
Fragments arranged by size:
2119 1510 742 714 146
EcoICRI GAG'CTC
Cuts at: 2243 2243
Size: 5231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 536 1265 3056 3488 3841 4503 159
Size: 377 729 1791 432 353 662 887
Fragments arranged by size:
1791 887 729 662 432 377 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1217 1217
Size: 5231
EcoO109I rG'GnC_Cy
Cuts at: 797 869 1438 2370 797
Size: 72 569 932 3658
Fragments arranged by size:
3658 932 569 72
EcoRI G'AATT_C
Cuts at: 860 2235 860
Size: 1375 3856
EcoRV GAT'ATC
Cuts at: 1824 1824
Size: 5231
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3250 3250
Size: 5231
FspI TGC'GCA
Cuts at: 3012 3012
Size: 5231
HaeII r_GCGC'y
Cuts at: 631 2665 2913 4143 4914 631
Size: 2034 248 1230 771 948
Fragments arranged by size:
2034 1230 948 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 501 533 1327 1359 1780 1792 1795 1812 1824
Size: 32 794 32 421 12 3 17 12
Cuts at: 1824 1827 3073 3103 3105 3135 501
Size: 3 1246 30 2 30 2597
Fragments arranged by size:
2597 1246 794 421 32 32 30 30
17 12 12 3 3 2
HincII GTy'rAC
Cuts at: 301 2270 301
Size: 1969 3262
KasI G'GCGC_C
Cuts at: 2661 2909 2661
Size: 248 4983
KpnI G_GTAC'C
Cuts at: 1226 2251 1226
Size: 1025 4206
MfeI C'AATT_G
Cuts at: 1508 1508
Size: 5231
MluI A'CGCG_T
Cuts at: 1944 4664 1944
Size: 2720 2511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1179 1557 3990 4174 1179
Size: 378 2433 184 2236
Fragments arranged by size:
2433 2236 378 184
MscI TGG'CCA
Cuts at: 1236 2992 1236
Size: 1756 3475
MslI CAynn'nnrTG
Cuts at: 353 365 920 3220 3347 353
Size: 12 555 2300 127 2237
Fragments arranged by size:
2300 2237 555 127 12
MspA1I CmG'CkG
Cuts at: 2342 2656 3016 3800 4045 2342
Size: 314 360 784 245 3528
Fragments arranged by size:
3528 784 360 314 245
NaeI GCC'GGC
Cuts at: 3412 3412
Size: 5231
NarI GG'CG_CC
Cuts at: 2662 2910 2662
Size: 248 4983
NdeI CA'TA_TG
Cuts at: 2222 2222
Size: 5231
NgoMIV G'CCGG_C
Cuts at: 3410 3410
Size: 5231
NheI G'CTAG_C
Cuts at: 772 772
Size: 5231
NspI r_CATG'y
Cuts at: 888 2284 2784 4387 888
Size: 1396 500 1603 1732
Fragments arranged by size:
1732 1603 1396 500
PciI A'CATG_T
Cuts at: 884 4383 884
Size: 3499 1732
PflMI CCAn_nnn'nTGG
Cuts at: 1232 1243 1434 1232
Size: 11 191 5029
Fragments arranged by size:
5029 191 11
PfoI T'CCnGG_A
Cuts at: 598 598
Size: 5231
PmeI GTTT'AAAC
Cuts at: 2230 2230
Size: 5231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 5199
PpuMI rG'GwC_Cy
Cuts at: 797 869 1438 797
Size: 72 569 4590
Fragments arranged by size:
4590 569 72
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1002 1034 1704 1736 1839 1871 1002
Size: 32 670 32 103 32 4362
Fragments arranged by size:
4362 670 103 32 32 32
PstI C_TGCA'G
Cuts at: 627 2278 2963 627
Size: 1651 685 2895
Fragments arranged by size:
2895 1651 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5231
PvuII CAG'CTG
Cuts at: 2656 3016 2656
Size: 360 4871
RsrII CG'GwC_CG
Cuts at: 3426 3426
Size: 5231
SacI G_AGCT'C
Cuts at: 2245 2245
Size: 5231
SalI G'TCGA_C
Cuts at: 2268 2268
Size: 5231
SanDI GG'GwC_CC
Cuts at: 797 797
Size: 5231
SapI GCTCTTCn'nnn_
Cuts at: 3254 3464 3254
Size: 210 5021
SbfI CC_TGCA'GG
Cuts at: 2278 2278
Size: 5231
ScaI AGT'ACT
Cuts at: 1857 4748 1857
Size: 2891 2340
SfcI C'TryA_G
Cuts at: 33 137 623 2274 2959 3927 4118 33
Size: 104 486 1651 685 968 191 1146
Fragments arranged by size:
1651 1146 968 685 486 191 104
SfoI GGC'GCC
Cuts at: 2663 2911 2663
Size: 248 4983
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5231
SmaI CCC'GGG
Cuts at: 2253 2253
Size: 5231
SmlI C'TyrA_G
Cuts at: 930 2116 2386 3301 4015 4277 930
Size: 1186 270 915 714 262 1884
Fragments arranged by size:
1884 1186 915 714 270 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5231
SphI G_CATG'C
Cuts at: 2284 2784 2284
Size: 500 4731
StyI C'CwwG_G
Cuts at: 2365 2719 2365
Size: 354 4877
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 160 2814 2947 3406 3615 160
Size: 2654 133 459 209 1776
Fragments arranged by size:
2654 1776 459 209 133
TatI w'GTAC_w
Cuts at: 464 1633 1855 4746 464
Size: 1169 222 2891 949
Fragments arranged by size:
2891 1169 949 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 963 995 4748 4780 963
Size: 32 3753 32 1414
Fragments arranged by size:
3753 1414 32 32
Tth111I GACn'n_nGTC
Cuts at: 1597 3028 1597
Size: 1431 3800
XbaI T'CTAG_A
Cuts at: 325 1837 2067 2262 325
Size: 1512 230 195 3294
Fragments arranged by size:
3294 1512 230 195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 850 2726 850
Size: 1876 3355
XmaI C'CCGG_G
Cuts at: 2251 2251
Size: 5231
XmnI GAAnn'nnTTC
Cuts at: 1763 1763
Size: 5231
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BsaXI BsgI
BsiWI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HindIII HpaI NcoI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI