Restriction map for
pF1KB3222
(Circular) (Six-base) MAPSORT of: pF1KB3222.seq Check: 8168 from: 1 to: 6050
pF1KB3222 6050 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 3088 3088
Size: 6050
Acc65I G'GTAC_C
Cuts at: 143 3066 143
Size: 2923 3127
AclI AA'CG_TT
Cuts at: 1742 5890 1742
Size: 4148 1902
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1278 2593 2614 2749 3875 4307 4660 5322 1278
Size: 1315 21 135 1126 432 353 662 2006
Fragments arranged by size:
2006 1315 1126 662 432 353 135 21
AflIII A'CryG_T
Cuts at: 405 691 3402 5202 5483 405
Size: 286 2711 1800 281 972
Fragments arranged by size:
2711 1800 972 286 281
AhdI GACnn_n'nnGTC
Cuts at: 837 837
Size: 6050
AleI CACnn'nnGTG
Cuts at: 772 1429 772
Size: 657 5393
AlwNI CAG_nnn'CTG
Cuts at: 2505 2847 2943 3355 4793 2505
Size: 342 96 412 1438 3762
Fragments arranged by size:
3762 1438 412 342 96
ApaLI G'TGCA_C
Cuts at: 763 1362 4888 763
Size: 599 3526 1925
Fragments arranged by size:
3526 1925 599
ApoI r'AATT_y
Cuts at: 178 1807 2613 3054 5403 5659 178
Size: 1629 806 441 2349 256 569
Fragments arranged by size:
2349 1629 806 569 441 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6050
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
AvaI C'yCGr_G
Cuts at: 231 1132 1466 3070 231
Size: 901 334 1604 3211
Fragments arranged by size:
3211 1604 901 334
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 457 490 457
Size: 33 6017
BamHI G'GATC_C
Cuts at: 3075 3075
Size: 6050
BanI G'GyrC_C
Cuts at: 143 1033 3066 3480 3728 3763 143
Size: 890 2033 414 248 35 2430
Fragments arranged by size:
2430 2033 890 414 248 35
BanII G_rGCy'C
Cuts at: 703 1514 3064 4094 703
Size: 811 1550 1030 2659
Fragments arranged by size:
2659 1550 1030 811
BbeI G_GCGC'C
Cuts at: 3484 3732 3484
Size: 248 5802
BbsI GAAGACnn'nnnn_
Cuts at: 443 569 1431 443
Size: 126 862 5062
Fragments arranged by size:
5062 862 126
BbvCI CC'TCA_GC
Cuts at: 2488 2488
Size: 6050
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 392 426 2688 2722 392
Size: 34 2262 34 3720
Fragments arranged by size:
3720 2262 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3948 5004 6050 3948
Size: 1056 1046 3948
Fragments arranged by size:
3948 1056 1046
BclI T'GATC_A
Cuts at: 932 3570 932
Size: 2638 3412
BglII A'GATC_T
Cuts at: 1904 3565 5602 1904
Size: 1661 2037 2352
Fragments arranged by size:
2352 2037 1661
BlpI GC'TnA_GC
Cuts at: 2857 3162 2857
Size: 305 5745
Bme1580I G_kGCm'C
Cuts at: 767 1366 3675 3768 4892 767
Size: 599 2309 93 1124 1925
Fragments arranged by size:
2309 1925 1124 599 93
BmgBI CAC'GTC
Cuts at: 692 1779 2798 692
Size: 1087 1019 3944
Fragments arranged by size:
3944 1087 1019
BmrI ACTGGGnnnn_n'
Cuts at: 843 3262 3343 3678 5822 843
Size: 2419 81 335 2144 1071
Fragments arranged by size:
2419 2144 1071 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1114 1890 4417 1114
Size: 776 2527 2747
Fragments arranged by size:
2747 2527 776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 178 490 975 1192 1945 2386 2820 169
Size: 9 312 485 217 753 441 434 3399
Fragments arranged by size:
3399 753 485 441 434 312 217 9
Bpu10I CC'TnA_GC
Cuts at: 1102 2488 3014 1102
Size: 1386 526 4138
Fragments arranged by size:
4138 1386 526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3226 4105 4819 5117 3226
Size: 879 714 298 4159
Fragments arranged by size:
4159 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 344 1152 5754 344
Size: 808 4602 640
Fragments arranged by size:
4602 808 640
BsaAI yAC'GTr
Cuts at: 406 537 3403 406
Size: 131 2866 3053
Fragments arranged by size:
3053 2866 131
BsaBI GATnn'nnATC
Cuts at: 6 2068 3564 5601 6
Size: 2062 1496 2037 455
Fragments arranged by size:
2062 2037 1496 455
BsaHI Gr'CG_yC
Cuts at: 838 3481 3729 4431 5927 838
Size: 2643 248 702 1496 961
Fragments arranged by size:
2643 1496 961 702 248
BsaWI w'CCGG_w
Cuts at: 638 3239 3760 4438 4849 4996 5270 638
Size: 2601 521 678 411 147 274 1418
Fragments arranged by size:
2601 1418 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 292 322 715 745 2128 2158 292
Size: 30 393 30 1383 30 4184
Fragments arranged by size:
4184 1383 393 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 466 930 1576 2038 2197 2200 2229 313
Size: 153 464 646 462 159 3 29 4134
Fragments arranged by size:
4134 646 464 462 159 153 29 3
BseYI C'CCAG_C
Cuts at: 1768 1981 2810 3475 4233 4898 5306 1768
Size: 213 829 665 758 665 408 2512
Fragments arranged by size:
2512 829 758 665 665 408 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1047 2239 1047
Size: 1192 4858
BsiEI CG_ry'CG
Cuts at: 104 3592 3638 4868 104
Size: 3488 46 1230 1286
Fragments arranged by size:
3488 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 638 767 1049 1366 1487 2323 3064 3842 4032
Size: 129 282 317 121 836 741 778 190
Cuts at: 4032 4892 638
Size: 860 1796
Fragments arranged by size:
1796 860 836 778 741 317 282 190
129 121
BsmI GAATG_Cn'
Cuts at: 576 750 576
Size: 174 5876
BsmBI CGTCTCn'nnnn_
Cuts at: 628 1769 628
Size: 1141 4909
Bsp1286I G_dGCh'C
Cuts at: 638 703 767 1049 1366 1487 1514 2323 3064
Size: 65 64 282 317 121 27 809 741
Cuts at: 3064 3675 3768 3842 4032 4094 4892 638
Size: 611 93 74 190 62 798 1796
Fragments arranged by size:
1796 809 798 741 611 317 282 190
121 93 74 65 64 62 27
BspHI T'CATG_A
Cuts at: 935 2894 4482 935
Size: 1959 1588 2503
Fragments arranged by size:
2503 1959 1588
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1718 2699 3100 3314 3616 3997 1718
Size: 981 401 214 302 381 3771
Fragments arranged by size:
3771 981 401 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1059 1086 4342 4396 5880 6045 50
Size: 1009 27 3256 54 1484 165 55
Fragments arranged by size:
3256 1484 1009 165 55 54 27
BsrDI GCAATG_nn'
Cuts at: 3962 3962
Size: 6050
BsrFI r'CCGG_y
Cuts at: 4048 4229 4048
Size: 181 5869
BssSI C'ACGA_G
Cuts at: 1155 4321 5029 1155
Size: 3166 708 2176
Fragments arranged by size:
3166 2176 708
BstAPI GCAn_nnn'nTGC
Cuts at: 128 1188 3427 128
Size: 1060 2239 2751
Fragments arranged by size:
2751 2239 1060
BstBI TT'CG_AA
Cuts at: 412 1811 4411 412
Size: 1399 2600 2051
Fragments arranged by size:
2600 2051 1399
BstXI CCAn_nnnn'nTGG
Cuts at: 114 147 720 1989 2088 2410 114
Size: 33 573 1269 99 322 3754
Fragments arranged by size:
3754 1269 573 322 99 33
BstYI r'GATC_y
Cuts at: 1 264 326 1214 1904 1973 2814 3075 3542
Size: 263 62 888 690 69 841 261 467
Cuts at: 3542 3565 3900 4146 4452 4464 4550 5602 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 888 841 690 467 449 335 306
263 261 246 86 69 62 23 12
Bsu36I CC'TnA_GG
Cuts at: 1573 1615 1573
Size: 42 6008
BtgI C'CryG_G
Cuts at: 314 770 1427 2045 314
Size: 456 657 618 4319
Fragments arranged by size:
4319 657 618 456
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4000 4181 4344 4000
Size: 181 163 5706
Fragments arranged by size:
5706 181 163
BtsI GCAGTG_nn'
Cuts at: 2227 2802 3293 2227
Size: 575 491 4984
Fragments arranged by size:
4984 575 491
DraI TTT'AAA
Cuts at: 3049 3049
Size: 6050
DraIII CAC_nnn'GTG
Cuts at: 771 1331 771
Size: 560 5490
DrdI GACnn_nn'nnGTC
Cuts at: 1783 2890 3756 5100 1783
Size: 1107 866 1344 2733
Fragments arranged by size:
2733 1344 1107 866
EaeI y'GGCC_r
Cuts at: 137 317 671 812 3635 3809 4200 4227 5572
Size: 180 354 141 2823 174 391 27 1345
Cuts at: 5572 137
Size: 615
Fragments arranged by size:
2823 1345 615 391 354 180 174 141
27
EagI C'GGCC_G
Cuts at: 3635 3635
Size: 6050
EarI CTCTTCn'nnn_
Cuts at: 443 1006 1327 1712 2174 4073 4283 443
Size: 563 321 385 462 1899 210 2210
Fragments arranged by size:
2210 1899 563 462 385 321 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1612 4258 5000 5146 5860 1612
Size: 2646 742 146 714 1802
Fragments arranged by size:
2646 1802 742 714 146
EcoICRI GAG'CTC
Cuts at: 3062 3062
Size: 6050
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 178 490 975 1192 1278 1945 2386 2593
Size: 9 312 485 217 86 667 441 207
Cuts at: 2593 2614 2749 2820 3875 4307 4660 5322 169
Size: 21 135 71 1055 432 353 662 897
Fragments arranged by size:
1055 897 667 662 485 441 432 353
312 217 207 135 86 71 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1558 1558
Size: 6050
EcoO109I rG'GnC_Cy
Cuts at: 153 927 1317 1871 2301 2370 3189 153
Size: 774 390 554 430 69 819 3014
Fragments arranged by size:
3014 819 774 554 430 390 69
EcoRI G'AATT_C
Cuts at: 2613 3054 2613
Size: 441 5609
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 207 4069 207
Size: 3862 2188
FspI TGC'GCA
Cuts at: 3831 3831
Size: 6050
HaeII r_GCGC'y
Cuts at: 2445 3484 3732 4962 5733 2445
Size: 1039 248 1230 771 2762
Fragments arranged by size:
2762 1230 1039 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 608 640 823 855 2128 2160 3892 3922 3924
Size: 32 183 32 1273 32 1732 30 2
Cuts at: 3924 3954 608
Size: 30 2704
Fragments arranged by size:
2704 1732 1273 183 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3089 3089
Size: 6050
HindIII A'AGCT_T
Cuts at: 1762 2761 1762
Size: 999 5051
KasI G'GCGC_C
Cuts at: 3480 3728 3480
Size: 248 5802
KpnI G_GTAC'C
Cuts at: 147 3070 147
Size: 2923 3127
MluI A'CGCG_T
Cuts at: 5483 5483
Size: 6050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 809 1592 2753 4809 4993 809
Size: 783 1161 2056 184 1866
Fragments arranged by size:
2056 1866 1161 783 184
MscI TGG'CCA
Cuts at: 139 673 814 3811 139
Size: 534 141 2997 2378
Fragments arranged by size:
2997 2378 534 141
MslI CAynn'nnrTG
Cuts at: 112 277 319 718 772 1429 1672 2086 4039
Size: 165 42 399 54 657 243 414 1953
Cuts at: 4039 4166 112
Size: 127 1996
Fragments arranged by size:
1996 1953 657 414 399 243 165 127
54 42
MspA1I CmG'CkG
Cuts at: 128 1429 1989 3161 3475 3835 4619 4864 128
Size: 1301 560 1172 314 360 784 245 1314
Fragments arranged by size:
1314 1301 1172 784 560 360 314 245
NaeI GCC'GGC
Cuts at: 4231 4231
Size: 6050
NarI GG'CG_CC
Cuts at: 3481 3729 3481
Size: 248 5802
NcoI C'CATG_G
Cuts at: 314 2045 314
Size: 1731 4319
NgoMIV G'CCGG_C
Cuts at: 4229 4229
Size: 6050
NspI r_CATG'y
Cuts at: 1210 2169 3103 3603 5206 1210
Size: 959 934 500 1603 2054
Fragments arranged by size:
2054 1603 959 934 500
PasI CC'CwG_GG
Cuts at: 2148 2374 2148
Size: 226 5824
PciI A'CATG_T
Cuts at: 5202 5202
Size: 6050
PflMI CCAn_nnn'nTGG
Cuts at: 2505 2505
Size: 6050
PfoI T'CCnGG_A
Cuts at: 1682 1682
Size: 6050
PmeI GTTT'AAAC
Cuts at: 3049 3049
Size: 6050
PmlI CAC'GTG
Cuts at: 406 406
Size: 6050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 4496
Size: 32 6018
PpuMI rG'GwC_Cy
Cuts at: 153 927 1317 1871 2370 153
Size: 774 390 554 499 3833
Fragments arranged by size:
3833 774 554 499 390
PshAI GACnn'nnGTC
Cuts at: 3025 3025
Size: 6050
PstI C_TGCA'G
Cuts at: 1184 1463 1530 1592 1973 2939 3097 3782 1184
Size: 279 67 62 381 966 158 685 3452
Fragments arranged by size:
3452 966 685 381 279 158 67 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6050
PvuII CAG'CTG
Cuts at: 1989 3475 3835 1989
Size: 1486 360 4204
Fragments arranged by size:
4204 1486 360
RsrII CG'GwC_CG
Cuts at: 4245 4245
Size: 6050
SacI G_AGCT'C
Cuts at: 3064 3064
Size: 6050
SacII CC_GC'GG
Cuts at: 1430 1430
Size: 6050
SalI G'TCGA_C
Cuts at: 3087 3087
Size: 6050
SanDI GG'GwC_CC
Cuts at: 2370 2370
Size: 6050
SapI GCTCTTCn'nnn_
Cuts at: 4073 4283 4073
Size: 210 5840
SbfI CC_TGCA'GG
Cuts at: 3097 3097
Size: 6050
ScaI AGT'ACT
Cuts at: 1249 1261 5567 1249
Size: 12 4306 1732
Fragments arranged by size:
4306 1732 12
SfcI C'TryA_G
Cuts at: 33 1180 1459 1526 1588 1659 1969 2935 3093
Size: 1147 279 67 62 71 310 966 158
Cuts at: 3093 3778 4746 4937 33
Size: 685 968 191 1146
Fragments arranged by size:
1147 1146 968 966 685 310 279 191
158 71 67 62
SfoI GGC'GCC
Cuts at: 3482 3730 3482
Size: 248 5802
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
SmaI CCC'GGG
Cuts at: 1468 3072 1468
Size: 1604 4446
SmlI C'TyrA_G
Cuts at: 3205 4120 4834 5096 3205
Size: 915 714 262 4159
Fragments arranged by size:
4159 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6050
SphI G_CATG'C
Cuts at: 3103 3603 3103
Size: 500 5550
StuI AGG'CCT
Cuts at: 1138 1138
Size: 6050
StyI C'CwwG_G
Cuts at: 314 2045 2171 2480 3184 3538 314
Size: 1731 126 309 704 354 2826
Fragments arranged by size:
2826 1731 704 354 309 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 978 3633 3766 4225 4434 571
Size: 407 2655 133 459 209 2187
Fragments arranged by size:
2655 2187 459 407 209 133
TatI w'GTAC_w
Cuts at: 524 1247 1259 1703 5565 524
Size: 723 12 444 3862 1009
Fragments arranged by size:
3862 1009 723 444 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 292 324 2269 2301 5567 5599 292
Size: 32 1945 32 3266 32 743
Fragments arranged by size:
3266 1945 743 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 620 3847 620
Size: 3227 2823
XbaI T'CTAG_A
Cuts at: 3081 3081
Size: 6050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1161 3545 1161
Size: 2384 3666
XmaI C'CCGG_G
Cuts at: 1466 3070 1466
Size: 1604 4446
XmnI GAAnn'nnTTC
Cuts at: 1627 2996 1627
Size: 1369 4681
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AloI ApaI
AscI AvrII BfrBI BglI BmtI BsiWI BspEI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI