Restriction map for
pF1KB3205
(Circular) (Six-base) MAPSORT of: pF1KB3205.seq Check: 7464 from: 1 to: 4748
pF1KB3205 4748 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 539 1491 1786 539
Size: 952 295 3501
Fragments arranged by size:
3501 952 295
Acc65I G'GTAC_C
Cuts at: 1764 1764
Size: 4748
AclI AA'CG_TT
Cuts at: 4588 4588
Size: 4748
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 2573 3005 3752 4020 982
Size: 1591 432 747 268 1710
Fragments arranged by size:
1710 1591 747 432 268
AflIII A'CryG_T
Cuts at: 223 2100 3204 4181 223
Size: 1877 1104 977 790
Fragments arranged by size:
1877 1104 977 790
AgeI A'CCGG_T
Cuts at: 3136 3136
Size: 4748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 841 841
Size: 4748
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 155 187 1262 1294 155
Size: 32 1075 32 3609
Fragments arranged by size:
3609 1075 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2053 3620 2053
Size: 1567 3181
ApaI G_GGCC'C
Cuts at: 1095 1095
Size: 4748
ApaLI G'TGCA_C
Cuts at: 3518 3518
Size: 4748
ApoI r'AATT_y
Cuts at: 905 1752 4101 4357 905
Size: 847 2349 256 1296
Fragments arranged by size:
2349 1296 847 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4748
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
AvaI C'yCGr_G
Cuts at: 1768 1768
Size: 4748
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1553 1586 1553
Size: 33 4715
BamHI G'GATC_C
Cuts at: 1773 1773
Size: 4748
BanI G'GyrC_C
Cuts at: 1329 1764 2178 2426 2461 1329
Size: 435 414 248 35 3616
Fragments arranged by size:
3616 435 414 248 35
BanII G_rGCy'C
Cuts at: 1095 1725 1762 2792 1095
Size: 630 37 1030 3051
Fragments arranged by size:
3051 1030 630 37
BbeI G_GCGC'C
Cuts at: 2182 2430 2182
Size: 248 4500
BbsI GAAGACnn'nnnn_
Cuts at: 743 1063 743
Size: 320 4428
BbvCI CC'TCA_GC
Cuts at: 1322 1322
Size: 4748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1282 1316 1282
Size: 34 4714
BciVI GTATCCnnnnn_n'
Cuts at: 541 2646 3407 4748 541
Size: 2105 761 1341 541
Fragments arranged by size:
2105 1341 761 541
BclI T'GATC_A
Cuts at: 473 506 2268 473
Size: 33 1762 2953
Fragments arranged by size:
2953 1762 33
BglI GCCn_nnn'nGGC
Cuts at: 1090 1715 1090
Size: 625 4123
BglII A'GATC_T
Cuts at: 2263 4300 2263
Size: 2037 2711
BlpI GC'TnA_GC
Cuts at: 136 434 1007 1860 136
Size: 298 573 853 3024
Fragments arranged by size:
3024 853 573 298
Bme1580I G_kGCm'C
Cuts at: 1095 2373 2466 3522 1095
Size: 1278 93 1056 2321
Fragments arranged by size:
2321 1278 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 529 1594 1960 2041 2376 4520 529
Size: 1065 366 81 335 2144 757
Fragments arranged by size:
2144 1065 757 366 335 81
BmtI G_CTAG'C
Cuts at: 1247 1247
Size: 4748
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1275 3115 1275
Size: 1840 2908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 743 895 1264 466
Size: 277 152 369 3950
Fragments arranged by size:
3950 369 277 152
Bpu10I CC'TnA_GC
Cuts at: 1322 1322
Size: 4748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1924 2803 3295 3593 1924
Size: 879 492 298 3079
Fragments arranged by size:
3079 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1528 4452 1528
Size: 2924 1824
BsaAI yAC'GTr
Cuts at: 790 2101 790
Size: 1311 3437
BsaBI GATnn'nnATC
Cuts at: 6 2262 4299 6
Size: 2256 2037 455
Fragments arranged by size:
2256 2037 455
BsaHI Gr'CG_yC
Cuts at: 2179 2427 3129 4625 2179
Size: 248 702 1496 2302
Fragments arranged by size:
2302 1496 702 248
BsaWI w'CCGG_w
Cuts at: 160 1052 1937 2458 3136 3410 3557 3968 160
Size: 892 885 521 678 274 147 411 940
Fragments arranged by size:
940 892 885 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 263 293 910 940 1262 1292 263
Size: 30 617 30 322 30 3719
Fragments arranged by size:
3719 617 322 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 679 904 1222 679
Size: 225 318 4205
Fragments arranged by size:
4205 318 225
BseYI C'CCAG_C
Cuts at: 2173 2931 3508 4004 2173
Size: 758 577 496 2917
Fragments arranged by size:
2917 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1206 1206
Size: 4748
BsiEI CG_ry'CG
Cuts at: 104 2290 2336 3544 104
Size: 2186 46 1208 1308
Fragments arranged by size:
2186 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 131 137 440 1762 2540 2730 3522 131
Size: 6 303 1322 778 190 792 1357
Fragments arranged by size:
1357 1322 792 778 303 190 6
BsmBI CGTCTCn'nnnn_
Cuts at: 1549 1633 1549
Size: 84 4664
Bsp1286I G_dGCh'C
Cuts at: 131 137 440 1095 1725 1762 2373 2466 2540
Size: 6 303 655 630 37 611 93 74
Cuts at: 2540 2730 2792 3522 131
Size: 190 62 730 1357
Fragments arranged by size:
1357 730 655 630 611 303 190 93
74 62 37 6
BspEI T'CCGG_A
Cuts at: 160 1052 3968 160
Size: 892 2916 940
Fragments arranged by size:
2916 940 892
BspHI T'CATG_A
Cuts at: 761 3924 761
Size: 3163 1585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1798 2012 2314 2695 1798
Size: 214 302 381 3851
Fragments arranged by size:
3851 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3040 3094 4578 4743 50
Size: 2990 54 1484 165 55
Fragments arranged by size:
2990 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 303 629 1073 1311 2660 303
Size: 326 444 238 1349 2391
Fragments arranged by size:
2391 1349 444 326 238
BsrFI r'CCGG_y
Cuts at: 1709 2746 2927 3136 1709
Size: 1037 181 209 3321
Fragments arranged by size:
3321 1037 209 181
BssSI C'ACGA_G
Cuts at: 130 884 3019 3377 130
Size: 754 2135 358 1501
Fragments arranged by size:
2135 1501 754 358
BstAPI GCAn_nnn'nTGC
Cuts at: 456 2125 456
Size: 1669 3079
BstBI TT'CG_AA
Cuts at: 3109 3109
Size: 4748
BstXI CCAn_nnnn'nTGG
Cuts at: 1471 1471
Size: 4748
BstYI r'GATC_y
Cuts at: 1 1773 2240 2263 2598 2844 3856 3942 3954
Size: 1772 467 23 335 246 1012 86 12
Cuts at: 3954 4300 1
Size: 346 449
Fragments arranged by size:
1772 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1712 2698 2879 3042 1712
Size: 986 181 163 3418
Fragments arranged by size:
3418 986 181 163
BtsI GCAGTG_nn'
Cuts at: 1640 1991 1640
Size: 351 4397
DraI TTT'AAA
Cuts at: 1747 1747
Size: 4748
DrdI GACnn_nn'nnGTC
Cuts at: 745 2454 3312 745
Size: 1709 858 2181
Fragments arranged by size:
2181 1709 858
EaeI y'GGCC_r
Cuts at: 335 1411 1734 2333 2507 2898 2925 4270 335
Size: 1076 323 599 174 391 27 1345 813
Fragments arranged by size:
1345 1076 813 599 391 323 174 27
EagI C'GGCC_G
Cuts at: 2333 2333
Size: 4748
EarI CTCTTCn'nnn_
Cuts at: 368 917 2771 2981 368
Size: 549 1854 210 2135
Fragments arranged by size:
2135 1854 549 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2956 3266 3412 4558 2956
Size: 310 146 1146 3146
Fragments arranged by size:
3146 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1760 1760
Size: 4748
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 743 895 982 1264 2573 3005 3752 4020
Size: 277 152 87 282 1309 432 747 268
Cuts at: 4020 466
Size: 1194
Fragments arranged by size:
1309 1194 747 432 282 277 268 152
87
EcoO109I rG'GnC_Cy
Cuts at: 232 1091 1343 1434 1887 232
Size: 859 252 91 453 3093
Fragments arranged by size:
3093 859 453 252 91
EcoRI G'AATT_C
Cuts at: 905 1752 905
Size: 847 3901
EcoRV GAT'ATC
Cuts at: 213 213
Size: 4748
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 2767
Size: 4748
FspI TGC'GCA
Cuts at: 2529 2529
Size: 4748
HaeII r_GCGC'y
Cuts at: 801 2182 2430 3452 4431 801
Size: 1381 248 1022 979 1118
Fragments arranged by size:
1381 1118 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 2590 2620 2622 2652 102
Size: 32 2456 30 2 30 2198
Fragments arranged by size:
2456 2198 32 30 30 2
HincII GTy'rAC
Cuts at: 540 1787 540
Size: 1247 3501
KasI G'GCGC_C
Cuts at: 2178 2426 2178
Size: 248 4500
KpnI G_GTAC'C
Cuts at: 1768 1768
Size: 4748
MluI A'CGCG_T
Cuts at: 4181 4181
Size: 4748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 360 655 981 1116 1136 3419 3603 360
Size: 295 326 135 20 2283 184 1505
Fragments arranged by size:
2283 1505 326 295 184 135 20
MscI TGG'CCA
Cuts at: 1413 1736 2509 1413
Size: 323 773 3652
Fragments arranged by size:
3652 773 323
MslI CAynn'nnrTG
Cuts at: 280 1294 2737 2864 280
Size: 1014 1443 127 2164
Fragments arranged by size:
2164 1443 1014 127
MspA1I CmG'CkG
Cuts at: 334 1326 1859 2173 2533 3546 3791 334
Size: 992 533 314 360 1013 245 1291
Fragments arranged by size:
1291 1013 992 533 360 314 245
NaeI GCC'GGC
Cuts at: 1711 2929 1711
Size: 1218 3530
NarI GG'CG_CC
Cuts at: 2179 2427 2179
Size: 248 4500
NdeI CA'TA_TG
Cuts at: 1591 1591
Size: 4748
NgoMIV G'CCGG_C
Cuts at: 1709 2927 1709
Size: 1218 3530
NheI G'CTAG_C
Cuts at: 1243 1243
Size: 4748
NspI r_CATG'y
Cuts at: 227 1801 2301 3208 227
Size: 1574 500 907 1767
Fragments arranged by size:
1767 1574 907 500
PciI A'CATG_T
Cuts at: 223 3204 223
Size: 2981 1767
PflMI CCAn_nnn'nTGG
Cuts at: 1339 1339
Size: 4748
PfoI T'CCnGG_A
Cuts at: 443 1280 1313 443
Size: 837 33 3878
Fragments arranged by size:
3878 837 33
PmeI GTTT'AAAC
Cuts at: 1747 1747
Size: 4748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1262 1294 3887 3919 1262
Size: 32 2593 32 2091
Fragments arranged by size:
2593 2091 32 32
PpuMI rG'GwC_Cy
Cuts at: 232 232
Size: 4748
PshAI GACnn'nnGTC
Cuts at: 237 237
Size: 4748
PsiI TTA'TAA
Cuts at: 1625 1625
Size: 4748
PspOMI G'GGCC_C
Cuts at: 1091 1091
Size: 4748
PstI C_TGCA'G
Cuts at: 1619 1795 2480 1619
Size: 176 685 3887
Fragments arranged by size:
3887 685 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4748
PvuII CAG'CTG
Cuts at: 1326 2173 2533 1326
Size: 847 360 3541
Fragments arranged by size:
3541 847 360
RsrII CG'GwC_CG
Cuts at: 2943 2943
Size: 4748
SacI G_AGCT'C
Cuts at: 1762 1762
Size: 4748
SalI G'TCGA_C
Cuts at: 538 1785 538
Size: 1247 3501
SanDI GG'GwC_CC
Cuts at: 232 232
Size: 4748
SapI GCTCTTCn'nnn_
Cuts at: 2771 2981 2771
Size: 210 4538
SbfI CC_TGCA'GG
Cuts at: 1795 1795
Size: 4748
ScaI AGT'ACT
Cuts at: 4265 4265
Size: 4748
SfcI C'TryA_G
Cuts at: 33 428 1615 1791 2476 3469 3660 33
Size: 395 1187 176 685 993 191 1121
Fragments arranged by size:
1187 1121 993 685 395 191 176
SfoI GGC'GCC
Cuts at: 2180 2428 2180
Size: 248 4500
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
SmaI CCC'GGG
Cuts at: 1770 1770
Size: 4748
SmlI C'TyrA_G
Cuts at: 1903 2818 3310 3572 1903
Size: 915 492 262 3079
Fragments arranged by size:
3079 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4748
SphI G_CATG'C
Cuts at: 1801 2301 1801
Size: 500 4248
StuI AGG'CCT
Cuts at: 1151 1219 1151
Size: 68 4680
StyI C'CwwG_G
Cuts at: 1214 1385 1882 2236 1214
Size: 171 497 354 3726
Fragments arranged by size:
3726 497 354 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 476 1534 2331 2464 2923 3132 476
Size: 1058 797 133 459 209 2092
Fragments arranged by size:
2092 1058 797 459 209 133
TatI w'GTAC_w
Cuts at: 926 4263 926
Size: 3337 1411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 263 295 4265 4297 263
Size: 32 3970 32 714
Fragments arranged by size:
3970 714 32 32
Tth111I GACn'n_nGTC
Cuts at: 2545 2545
Size: 4748
XbaI T'CTAG_A
Cuts at: 1779 1779
Size: 4748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1398 2243 1398
Size: 845 3903
XmaI C'CCGG_G
Cuts at: 1768 1768
Size: 4748
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BfrBI BmgBI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NotI NruI NsiI PacI
PasI PmlI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI