Restriction map for
pF1KB3200
(Circular) (Six-base) MAPSORT of: pF1KB3200.seq Check: 7670 from: 1 to: 5150
pF1KB3200 5150 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2188 2188
Size: 5150
Acc65I G'GTAC_C
Cuts at: 2166 2166
Size: 5150
AclI AA'CG_TT
Cuts at: 4990 4990
Size: 5150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 521 1006 1835 1883 2011 2062 2975 3407
Size: 165 485 829 48 128 51 913 432
Cuts at: 3407 3760 4422 356
Size: 353 662 1084
Fragments arranged by size:
1084 913 829 662 485 432 353 165
128 51 48
AflIII A'CryG_T
Cuts at: 1698 2502 4302 4583 1698
Size: 804 1800 281 2265
Fragments arranged by size:
2265 1800 804 281
AhdI GACnn_n'nnGTC
Cuts at: 433 433
Size: 5150
AleI CACnn'nnGTG
Cuts at: 1408 1408
Size: 5150
AlwNI CAG_nnn'CTG
Cuts at: 784 2455 3893 784
Size: 1671 1438 2041
Fragments arranged by size:
2041 1671 1438
ApaI G_GGCC'C
Cuts at: 169 169
Size: 5150
ApaLI G'TGCA_C
Cuts at: 3988 3988
Size: 5150
ApoI r'AATT_y
Cuts at: 875 1628 1900 2154 4503 4759 875
Size: 753 272 254 2349 256 1266
Fragments arranged by size:
2349 1266 753 272 256 254
AseI AT'TA_AT
Cuts at: 20 767 1481 20
Size: 747 714 3689
Fragments arranged by size:
3689 747 714
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5150
AvaI C'yCGr_G
Cuts at: 161 2170 161
Size: 2009 3141
BamHI G'GATC_C
Cuts at: 2175 2175
Size: 5150
BanI G'GyrC_C
Cuts at: 148 355 2166 2580 2828 2863 148
Size: 207 1811 414 248 35 2435
Fragments arranged by size:
2435 1811 414 248 207 35
BanII G_rGCy'C
Cuts at: 169 776 1326 1970 2164 3194 169
Size: 607 550 644 194 1030 2125
Fragments arranged by size:
2125 1030 644 607 550 194
BbeI G_GCGC'C
Cuts at: 152 2584 2832 152
Size: 2432 248 2470
Fragments arranged by size:
2470 2432 248
BbsI GAAGACnn'nnnn_
Cuts at: 821 941 1745 1826 821
Size: 120 804 81 4145
Fragments arranged by size:
4145 804 120 81
BciVI GTATCCnnnnn_n'
Cuts at: 909 3048 4104 5150 909
Size: 2139 1056 1046 909
Fragments arranged by size:
2139 1056 1046 909
BclI T'GATC_A
Cuts at: 677 2670 677
Size: 1993 3157
BfrBI ATG'CAT
Cuts at: 247 247
Size: 5150
BglII A'GATC_T
Cuts at: 2665 4702 2665
Size: 2037 3113
BlpI GC'TnA_GC
Cuts at: 2262 2262
Size: 5150
Bme1580I G_kGCm'C
Cuts at: 169 659 2775 2868 3992 169
Size: 490 2116 93 1124 1327
Fragments arranged by size:
2116 1327 1124 490 93
BmgBI CAC'GTC
Cuts at: 1729 1729
Size: 5150
BmrI ACTGGGnnnn_n'
Cuts at: 2117 2362 2443 2778 4922 2117
Size: 245 81 335 2144 2345
Fragments arranged by size:
2345 2144 335 245 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3517 3517
Size: 5150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 1610 361
Size: 1249 3901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2326 3205 3919 4217 2326
Size: 879 714 298 3259
Fragments arranged by size:
3259 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4854 4854
Size: 5150
BsaAI yAC'GTr
Cuts at: 2503 2503
Size: 5150
BsaBI GATnn'nnATC
Cuts at: 6 1744 2664 4701 6
Size: 1738 920 2037 455
Fragments arranged by size:
2037 1738 920 455
BsaHI Gr'CG_yC
Cuts at: 149 2581 2829 3531 5027 149
Size: 2432 248 702 1496 272
Fragments arranged by size:
2432 1496 702 272 248
BsaWI w'CCGG_w
Cuts at: 2339 2860 3538 3949 4096 4370 2339
Size: 521 678 411 147 274 3119
Fragments arranged by size:
3119 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 257 287 599 629 1182 1212 257
Size: 30 312 30 553 30 4195
Fragments arranged by size:
4195 553 312 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1056 1056
Size: 5150
BseYI C'CCAG_C
Cuts at: 687 2575 3333 3998 4406 687
Size: 1888 758 665 408 1431
Fragments arranged by size:
1888 1431 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1096
Size: 5150
BsiEI CG_ry'CG
Cuts at: 104 176 2692 2738 3968 104
Size: 72 2516 46 1230 1286
Fragments arranged by size:
2516 1286 1230 72 46
BsiHKAI G_wGCw'C
Cuts at: 1155 1970 2164 2942 3132 3992 1155
Size: 815 194 778 190 860 2313
Fragments arranged by size:
2313 860 815 778 194 190
BsmI GAATG_Cn'
Cuts at: 351 419 351
Size: 68 5082
BsmBI CGTCTCn'nnnn_
Cuts at: 145 478 145
Size: 333 4817
Bsp1286I G_dGCh'C
Cuts at: 169 659 776 1155 1326 1970 2164 2775 2868
Size: 490 117 379 171 644 194 611 93
Cuts at: 2868 2942 3132 3194 3992 169
Size: 74 190 62 798 1327
Fragments arranged by size:
1327 798 644 611 490 379 194 190
171 117 93 74 62
BspHI T'CATG_A
Cuts at: 210 3582 210
Size: 3372 1778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1926 2200 2414 2716 3097 1926
Size: 274 214 302 381 3979
Fragments arranged by size:
3979 381 302 274 214
BsrBI CCG'CTC
Cuts at: 50 3442 3496 4980 5145 50
Size: 3392 54 1484 165 55
Fragments arranged by size:
3392 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 824 3062 824
Size: 2238 2912
BsrFI r'CCGG_y
Cuts at: 417 482 1393 1823 3148 3329 417
Size: 65 911 430 1325 181 2238
Fragments arranged by size:
2238 1325 911 430 181 65
BsrGI T'GTAC_A
Cuts at: 1302 1302
Size: 5150
BssSI C'ACGA_G
Cuts at: 142 1643 3421 4129 142
Size: 1501 1778 708 1163
Fragments arranged by size:
1778 1501 1163 708
BstAPI GCAn_nnn'nTGC
Cuts at: 670 2527 670
Size: 1857 3293
BstBI TT'CG_AA
Cuts at: 2051 3511 2051
Size: 1460 3690
BstXI CCAn_nnnn'nTGG
Cuts at: 742 1756 742
Size: 1014 4136
BstYI r'GATC_y
Cuts at: 1 1308 2175 2642 2665 3000 3246 3552 3564
Size: 1307 867 467 23 335 246 306 12
Cuts at: 3564 3650 4702 1
Size: 86 1052 449
Fragments arranged by size:
1307 1052 867 467 449 335 306 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1412 3100 3281 3444 1412
Size: 1688 181 163 3118
Fragments arranged by size:
3118 1688 181 163
BtsI GCAGTG_nn'
Cuts at: 2393 2393
Size: 5150
DraI TTT'AAA
Cuts at: 534 2149 534
Size: 1615 3535
DraIII CAC_nnn'GTG
Cuts at: 966 966
Size: 5150
DrdI GACnn_nn'nnGTC
Cuts at: 2856 4200 2856
Size: 1344 3806
EaeI y'GGCC_r
Cuts at: 173 419 480 732 1358 1391 2735 2909 3300
Size: 246 61 252 626 33 1344 174 391
Cuts at: 3300 3327 4672 173
Size: 27 1345 651
Fragments arranged by size:
1345 1344 651 626 391 252 246 174
61 33 27
EagI C'GGCC_G
Cuts at: 173 2735 173
Size: 2562 2588
EarI CTCTTCn'nnn_
Cuts at: 1504 1940 2030 3173 3383 1504
Size: 436 90 1143 210 3271
Fragments arranged by size:
3271 1143 436 210 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3358 4100 4246 4960 3358
Size: 742 146 714 3548
Fragments arranged by size:
3548 742 714 146
EcoICRI GAG'CTC
Cuts at: 1968 2162 1968
Size: 194 4956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 361 521 1006 1610 1835 1883 2011 2062
Size: 5 160 485 604 225 48 128 51
Cuts at: 2062 2975 3407 3760 4422 356
Size: 913 432 353 662 1084
Fragments arranged by size:
1084 913 662 604 485 432 353 225
160 128 51 48 5
EcoO109I rG'GnC_Cy
Cuts at: 165 166 2289 165
Size: 1 2123 3026
Fragments arranged by size:
3026 2123 1
EcoRI G'AATT_C
Cuts at: 1628 1900 2154 1628
Size: 272 254 4624
Fragments arranged by size:
4624 272 254
EcoRV GAT'ATC
Cuts at: 740 740
Size: 5150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1276 3169 1276
Size: 1893 3257
FspI TGC'GCA
Cuts at: 2931 2931
Size: 5150
HaeII r_GCGC'y
Cuts at: 152 2584 2832 4062 4833 152
Size: 2432 248 1230 771 469
Fragments arranged by size:
2432 1230 771 469 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 419 451 886 918 1137 1169 1182
Size: 32 155 32 435 32 219 32 13
Cuts at: 1182 1214 2992 3022 3024 3054 232
Size: 32 1778 30 2 30 2328
Fragments arranged by size:
2328 1778 435 219 155 32 32 32
32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 1145 2189 1145
Size: 1044 4106
KasI G'GCGC_C
Cuts at: 148 2580 2828 148
Size: 2432 248 2470
Fragments arranged by size:
2470 2432 248
KpnI G_GTAC'C
Cuts at: 2170 2170
Size: 5150
MluI A'CGCG_T
Cuts at: 4583 4583
Size: 5150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 390 1525 3909 4093 390
Size: 1135 2384 184 1447
Fragments arranged by size:
2384 1447 1135 184
MscI TGG'CCA
Cuts at: 734 1360 2911 734
Size: 626 1551 2973
Fragments arranged by size:
2973 1551 626
MslI CAynn'nnrTG
Cuts at: 1408 3139 3266 1408
Size: 1731 127 3292
Fragments arranged by size:
3292 1731 127
MspA1I CmG'CkG
Cuts at: 781 1534 2261 2575 2935 3719 3964 781
Size: 753 727 314 360 784 245 1967
Fragments arranged by size:
1967 784 753 727 360 314 245
NaeI GCC'GGC
Cuts at: 419 484 1395 3331 419
Size: 65 911 1936 2238
Fragments arranged by size:
2238 1936 911 65
NarI GG'CG_CC
Cuts at: 149 2581 2829 149
Size: 2432 248 2470
Fragments arranged by size:
2470 2432 248
NgoMIV G'CCGG_C
Cuts at: 417 482 1393 3329 417
Size: 65 911 1936 2238
Fragments arranged by size:
2238 1936 911 65
NsiI A_TGCA'T
Cuts at: 249 249
Size: 5150
NspI r_CATG'y
Cuts at: 1702 2203 2703 4306 1702
Size: 501 500 1603 2546
Fragments arranged by size:
2546 1603 501 500
PciI A'CATG_T
Cuts at: 1698 4302 1698
Size: 2604 2546
PfoI T'CCnGG_A
Cuts at: 869 869
Size: 5150
PmeI GTTT'AAAC
Cuts at: 2149 2149
Size: 5150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 5118
PspOMI G'GGCC_C
Cuts at: 165 165
Size: 5150
PstI C_TGCA'G
Cuts at: 132 642 2197 2882 132
Size: 510 1555 685 2400
Fragments arranged by size:
2400 1555 685 510
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5150
PvuII CAG'CTG
Cuts at: 781 2575 2935 781
Size: 1794 360 2996
Fragments arranged by size:
2996 1794 360
RsrII CG'GwC_CG
Cuts at: 3345 3345
Size: 5150
SacI G_AGCT'C
Cuts at: 1970 2164 1970
Size: 194 4956
SalI G'TCGA_C
Cuts at: 2187 2187
Size: 5150
SapI GCTCTTCn'nnn_
Cuts at: 1504 3173 3383 1504
Size: 1669 210 3271
Fragments arranged by size:
3271 1669 210
SbfI CC_TGCA'GG
Cuts at: 2197 2197
Size: 5150
ScaI AGT'ACT
Cuts at: 4667 4667
Size: 5150
SfcI C'TryA_G
Cuts at: 33 128 638 2193 2878 3846 4037 33
Size: 95 510 1555 685 968 191 1146
Fragments arranged by size:
1555 1146 968 685 510 191 95
SfoI GGC'GCC
Cuts at: 150 2582 2830 150
Size: 2432 248 2470
Fragments arranged by size:
2470 2432 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5150
SmaI CCC'GGG
Cuts at: 163 2172 163
Size: 2009 3141
SmlI C'TyrA_G
Cuts at: 2305 3220 3934 4196 2305
Size: 915 714 262 3259
Fragments arranged by size:
3259 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5150
SphI G_CATG'C
Cuts at: 2203 2703 2203
Size: 500 4650
SspI AAT'ATT
Cuts at: 2004 2004
Size: 5150
StuI AGG'CCT
Cuts at: 949 949
Size: 5150
StyI C'CwwG_G
Cuts at: 1286 1605 2284 2638 1286
Size: 319 679 354 3798
Fragments arranged by size:
3798 679 354 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1255 2733 2866 3325 3534 1255
Size: 1478 133 459 209 2871
Fragments arranged by size:
2871 1478 459 209 133
TatI w'GTAC_w
Cuts at: 504 1302 1695 4665 504
Size: 798 393 2970 989
Fragments arranged by size:
2970 989 798 393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 599 631 737 769 4667 4699 599
Size: 32 106 32 3898 32 1050
Fragments arranged by size:
3898 1050 106 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 820 2947 820
Size: 2127 3023
XbaI T'CTAG_A
Cuts at: 801 2181 801
Size: 1380 3770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 303 1756 2645 303
Size: 1453 889 2808
Fragments arranged by size:
2808 1453 889
XmaI C'CCGG_G
Cuts at: 161 2170 161
Size: 2009 3141
XmnI GAAnn'nnTTC
Cuts at: 973 1256 2047 973
Size: 283 791 4076
Fragments arranged by size:
4076 791 283
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsiWI
BspEI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
EcoNI FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI