Restriction map for
pF1KB3191
(Circular) (Six-base) MAPSORT of: pF1KB3191.seq Check: 4801 from: 1 to: 4880
pF1KB3191 4880 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 1832 1832
Size: 4880
AccI GT'mk_AC
Cuts at: 1918 1918
Size: 4880
Acc65I G'GTAC_C
Cuts at: 1896 1896
Size: 4880
AclI AA'CG_TT
Cuts at: 4720 4720
Size: 4880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 1246 1525 2705 3137 3490 4152 884
Size: 362 279 1180 432 353 662 1612
Fragments arranged by size:
1612 1180 662 432 362 353 279
AflIII A'CryG_T
Cuts at: 1125 2232 4032 4313 1125
Size: 1107 1800 281 1692
Fragments arranged by size:
1800 1692 1107 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1642 1642
Size: 4880
AlwNI CAG_nnn'CTG
Cuts at: 810 975 1317 2185 3623 810
Size: 165 342 868 1438 2067
Fragments arranged by size:
2067 1438 868 342 165
ApaI G_GGCC'C
Cuts at: 1542 1754 1542
Size: 212 4668
ApaLI G'TGCA_C
Cuts at: 364 3718 364
Size: 3354 1526
ApoI r'AATT_y
Cuts at: 1884 4233 4489 1884
Size: 2349 256 2275
Fragments arranged by size:
2349 2275 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4880
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
AvaI C'yCGr_G
Cuts at: 575 1900 575
Size: 1325 3555
BamHI G'GATC_C
Cuts at: 1905 1905
Size: 4880
BanI G'GyrC_C
Cuts at: 1896 2310 2558 2593 1896
Size: 414 248 35 4183
Fragments arranged by size:
4183 414 248 35
BanII G_rGCy'C
Cuts at: 917 1542 1754 1894 2924 917
Size: 625 212 140 1030 2873
Fragments arranged by size:
2873 1030 625 212 140
BbeI G_GCGC'C
Cuts at: 2314 2562 2314
Size: 248 4632
BbsI GAAGACnn'nnnn_
Cuts at: 872 872
Size: 4880
BbvCI CC'TCA_GC
Cuts at: 1343 1343
Size: 4880
BciVI GTATCCnnnnn_n'
Cuts at: 2778 3834 4880 2778
Size: 1056 1046 2778
Fragments arranged by size:
2778 1056 1046
BclI T'GATC_A
Cuts at: 1070 2400 1070
Size: 1330 3550
BglI GCCn_nnn'nGGC
Cuts at: 112 1849 112
Size: 1737 3143
BglII A'GATC_T
Cuts at: 1082 2395 4432 1082
Size: 1313 2037 1530
Fragments arranged by size:
2037 1530 1313
BlpI GC'TnA_GC
Cuts at: 1220 1992 1220
Size: 772 4108
Bme1580I G_kGCm'C
Cuts at: 368 1240 1542 1754 2505 2598 3722 368
Size: 872 302 212 751 93 1124 1526
Fragments arranged by size:
1526 1124 872 751 302 212 93
BmrI ACTGGGnnnn_n'
Cuts at: 1305 2092 2173 2508 4652 1305
Size: 787 81 335 2144 1533
Fragments arranged by size:
2144 1533 787 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 777 3247 777
Size: 2470 2410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 499 715 847 901 406
Size: 93 216 132 54 4385
Fragments arranged by size:
4385 216 132 93 54
Bpu10I CC'TnA_GC
Cuts at: 1343 1343
Size: 4880
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2056 2935 3649 3947 2056
Size: 879 714 298 2989
Fragments arranged by size:
2989 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1165 1574 4584 1165
Size: 409 3010 1461
Fragments arranged by size:
3010 1461 409
BsaAI yAC'GTr
Cuts at: 363 2233 363
Size: 1870 3010
BsaBI GATnn'nnATC
Cuts at: 6 1294 2394 4431 6
Size: 1288 1100 2037 455
Fragments arranged by size:
2037 1288 1100 455
BsaHI Gr'CG_yC
Cuts at: 1349 1829 2311 2559 3261 4757 1349
Size: 480 482 248 702 1496 1472
Fragments arranged by size:
1496 1472 702 482 480 248
BsaWI w'CCGG_w
Cuts at: 617 1576 2069 2590 3268 3679 3826 4100 617
Size: 959 493 521 678 411 147 274 1397
Fragments arranged by size:
1397 959 678 521 493 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1093 1123 1093
Size: 30 4850
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 421 421
Size: 4880
BseYI C'CCAG_C
Cuts at: 324 672 803 1407 1693 2305 3063 3728 4136
Size: 348 131 604 286 612 758 665 408
Cuts at: 4136 324
Size: 1068
Fragments arranged by size:
1068 758 665 612 604 408 348 286
131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1579 1579
Size: 4880
BsiEI CG_ry'CG
Cuts at: 104 119 2422 2468 3698 104
Size: 15 2303 46 1230 1286
Fragments arranged by size:
2303 1286 1230 46 15
BsiHKAI G_wGCw'C
Cuts at: 155 368 464 631 917 1199 1894 2672 2862
Size: 213 96 167 286 282 695 778 190
Cuts at: 2862 3722 155
Size: 860 1313
Fragments arranged by size:
1313 860 778 695 286 282 213 190
167 96
Bsp1286I G_dGCh'C
Cuts at: 155 368 464 631 917 1199 1240 1542 1754
Size: 213 96 167 286 282 41 302 212
Cuts at: 1754 1894 2505 2598 2672 2862 2924 3722 155
Size: 140 611 93 74 190 62 798 1313
Fragments arranged by size:
1313 798 611 302 286 282 213 212
190 167 140 96 93 74 62 41
BspEI T'CCGG_A
Cuts at: 617 1576 617
Size: 959 3921
BspHI T'CATG_A
Cuts at: 3312 3312
Size: 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1601 1930 2144 2446 2827 1601
Size: 329 214 302 381 3654
Fragments arranged by size:
3654 381 329 302 214
BsrBI CCG'CTC
Cuts at: 50 3172 3226 4710 4875 50
Size: 3122 54 1484 165 55
Fragments arranged by size:
3122 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 177 2792 177
Size: 2615 2265
BsrFI r'CCGG_y
Cuts at: 2878 3059 2878
Size: 181 4699
BssSI C'ACGA_G
Cuts at: 457 3151 3859 457
Size: 2694 708 1478
Fragments arranged by size:
2694 1478 708
BstAPI GCAn_nnn'nTGC
Cuts at: 372 492 1203 2257 372
Size: 120 711 1054 2995
Fragments arranged by size:
2995 1054 711 120
BstBI TT'CG_AA
Cuts at: 3241 3241
Size: 4880
BstXI CCAn_nnnn'nTGG
Cuts at: 225 693 1566 225
Size: 468 873 3539
Fragments arranged by size:
3539 873 468
BstYI r'GATC_y
Cuts at: 1 822 1082 1905 2372 2395 2730 2976 3282
Size: 821 260 823 467 23 335 246 306
Cuts at: 3282 3294 3380 4432 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 823 821 467 449 335 306 260
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1241 1241
Size: 4880
BtgI C'CryG_G
Cuts at: 107 644 1676 107
Size: 537 1032 3311
Fragments arranged by size:
3311 1032 537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 129 204 783 2830 3011 3174 129
Size: 75 579 2047 181 163 1835
Fragments arranged by size:
2047 1835 579 181 163 75
BtsI GCAGTG_nn'
Cuts at: 366 437 932 2123 366
Size: 71 495 1191 3123
Fragments arranged by size:
3123 1191 495 71
DraI TTT'AAA
Cuts at: 1879 1879
Size: 4880
DraIII CAC_nnn'GTG
Cuts at: 492 492
Size: 4880
DrdI GACnn_nn'nnGTC
Cuts at: 1863 2586 3930 1863
Size: 723 1344 2813
Fragments arranged by size:
2813 1344 723
EaeI y'GGCC_r
Cuts at: 116 395 1370 1568 1727 2465 2639 3030 3057
Size: 279 975 198 159 738 174 391 27
Cuts at: 3057 4402 116
Size: 1345 594
Fragments arranged by size:
1345 975 738 594 391 279 198 174
159 27
EagI C'GGCC_G
Cuts at: 116 2465 116
Size: 2349 2531
EarI CTCTTCn'nnn_
Cuts at: 562 1216 1618 2903 3113 562
Size: 654 402 1285 210 2329
Fragments arranged by size:
2329 1285 654 402 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 613 3088 3830 3976 4690 613
Size: 2475 742 146 714 803
Fragments arranged by size:
2475 803 742 714 146
EcoICRI GAG'CTC
Cuts at: 915 1892 915
Size: 977 3903
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 499 715 847 884 901 1246 1525 2705
Size: 93 216 132 37 17 345 279 1180
Cuts at: 2705 3137 3490 4152 406
Size: 432 353 662 1134
Fragments arranged by size:
1180 1134 662 432 353 345 279 216
132 93 37 17
EcoO109I rG'GnC_Cy
Cuts at: 245 300 831 931 1698 1750 2019 245
Size: 55 531 100 767 52 269 3106
Fragments arranged by size:
3106 767 531 269 100 55 52
EcoRI G'AATT_C
Cuts at: 1884 1884
Size: 4880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 4880
FspI TGC'GCA
Cuts at: 1687 2661 1687
Size: 974 3906
HaeII r_GCGC'y
Cuts at: 2314 2562 3792 4563 2314
Size: 248 1230 771 2631
Fragments arranged by size:
2631 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 831 863 1093 1125 2722 2752 2754 2784 831
Size: 32 230 32 1597 30 2 30 2927
Fragments arranged by size:
2927 1597 230 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1919 1919
Size: 4880
KasI G'GCGC_C
Cuts at: 2310 2558 2310
Size: 248 4632
KpnI G_GTAC'C
Cuts at: 1900 1900
Size: 4880
MluI A'CGCG_T
Cuts at: 4313 4313
Size: 4880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 761 3639 3823 761
Size: 2878 184 1818
Fragments arranged by size:
2878 1818 184
MscI TGG'CCA
Cuts at: 397 1372 1570 1729 2641 397
Size: 975 198 159 912 2636
Fragments arranged by size:
2636 975 912 198 159
MslI CAynn'nnrTG
Cuts at: 148 643 727 1472 1624 2869 2996 148
Size: 495 84 745 152 1245 127 2032
Fragments arranged by size:
2032 1245 745 495 152 127 84
MspA1I CmG'CkG
Cuts at: 324 384 862 1051 1224 1305 1407 1991 2305
Size: 60 478 189 173 81 102 584 314
Cuts at: 2305 2665 3449 3694 324
Size: 360 784 245 1510
Fragments arranged by size:
1510 784 584 478 360 314 245 189
173 102 81 60
NaeI GCC'GGC
Cuts at: 3061 3061
Size: 4880
NarI GG'CG_CC
Cuts at: 2311 2559 2311
Size: 248 4632
NcoI C'CATG_G
Cuts at: 107 644 107
Size: 537 4343
NgoMIV G'CCGG_C
Cuts at: 3059 3059
Size: 4880
NspI r_CATG'y
Cuts at: 1129 1389 1671 1933 2433 4036 1129
Size: 260 282 262 500 1603 1973
Fragments arranged by size:
1973 1603 500 282 262 260
PciI A'CATG_T
Cuts at: 1125 4032 1125
Size: 2907 1973
PflMI CCAn_nnn'nTGG
Cuts at: 1824 1824
Size: 4880
PfoI T'CCnGG_A
Cuts at: 1757 1757
Size: 4880
PmeI GTTT'AAAC
Cuts at: 1879 1879
Size: 4880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 613 645 1246 1278 3326 3358 613
Size: 32 601 32 2048 32 2135
Fragments arranged by size:
2135 2048 601 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 245 300 1698 245
Size: 55 1398 3427
Fragments arranged by size:
3427 1398 55
PspOMI G'GGCC_C
Cuts at: 1538 1750 1538
Size: 212 4668
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 321 353 321
Size: 32 4848
PstI C_TGCA'G
Cuts at: 1742 1927 2612 1742
Size: 185 685 4010
Fragments arranged by size:
4010 685 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4880
PvuII CAG'CTG
Cuts at: 324 1051 1224 1305 1407 2305 2665 324
Size: 727 173 81 102 898 360 2539
Fragments arranged by size:
2539 898 727 360 173 102 81
RsrII CG'GwC_CG
Cuts at: 3075 3075
Size: 4880
SacI G_AGCT'C
Cuts at: 917 1894 917
Size: 977 3903
SalI G'TCGA_C
Cuts at: 1917 1917
Size: 4880
SanDI GG'GwC_CC
Cuts at: 1698 1698
Size: 4880
SapI GCTCTTCn'nnn_
Cuts at: 1618 2903 3113 1618
Size: 1285 210 3385
Fragments arranged by size:
3385 1285 210
SbfI CC_TGCA'GG
Cuts at: 1927 1927
Size: 4880
ScaI AGT'ACT
Cuts at: 613 4397 613
Size: 3784 1096
SexAI A'CCwGG_T
Cuts at: 953 953
Size: 4880
SfcI C'TryA_G
Cuts at: 33 1738 1923 2608 3576 3767 33
Size: 1705 185 685 968 191 1146
Fragments arranged by size:
1705 1146 968 685 191 185
SfoI GGC'GCC
Cuts at: 2312 2560 2312
Size: 248 4632
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
SmaI CCC'GGG
Cuts at: 1902 1902
Size: 4880
SmlI C'TyrA_G
Cuts at: 2035 2950 3664 3926 2035
Size: 915 714 262 2989
Fragments arranged by size:
2989 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4880
SphI G_CATG'C
Cuts at: 1933 2433 1933
Size: 500 4380
StyI C'CwwG_G
Cuts at: 107 224 231 644 686 1559 2014 2368 107
Size: 117 7 413 42 873 455 354 2619
Fragments arranged by size:
2619 873 455 413 354 117 42 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2463 2596 3055 3264 2463
Size: 133 459 209 4079
Fragments arranged by size:
4079 459 209 133
TatI w'GTAC_w
Cuts at: 611 1382 4395 611
Size: 771 3013 1096
Fragments arranged by size:
3013 1096 771
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 237 269 1339 1371 4397 4429 237
Size: 32 1070 32 3026 32 688
Fragments arranged by size:
3026 1070 688 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 706 955 2677 706
Size: 249 1722 2909
Fragments arranged by size:
2909 1722 249
XbaI T'CTAG_A
Cuts at: 1911 1911
Size: 4880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 222 951 1335 2375 222
Size: 729 384 1040 2727
Fragments arranged by size:
2727 1040 729 384
XmaI C'CCGG_G
Cuts at: 1900 1900
Size: 4880
ZraI GAC'GTC
Cuts at: 1830 1830
Size: 4880
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI SacII SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI