Restriction map for
pF1KB3144
(Circular) (Six-base) MAPSORT of: pF1KB3144.seq Check: 5173 from: 1 to: 4838
pF1KB3144 4838 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 555 686 690 555
Size: 131 4 4703
Fragments arranged by size:
4703 131 4
AccI GT'mk_AC
Cuts at: 1876 1876
Size: 4838
Acc65I G'GTAC_C
Cuts at: 1854 1854
Size: 4838
AclI AA'CG_TT
Cuts at: 1534 4678 1534
Size: 3144 1694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1294 1751 1797 2663 3095 3448 4110 1294
Size: 457 46 866 432 353 662 2022
Fragments arranged by size:
2022 866 662 457 432 353 46
AfeI AGC'GCT
Cuts at: 1321 1321
Size: 4838
AflIII A'CryG_T
Cuts at: 2190 3990 4271 2190
Size: 1800 281 2757
Fragments arranged by size:
2757 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1601 1601
Size: 4838
AlwNI CAG_nnn'CTG
Cuts at: 714 789 2143 3581 714
Size: 75 1354 1438 1971
Fragments arranged by size:
1971 1438 1354 75
ApaI G_GGCC'C
Cuts at: 163 477 734 773 163
Size: 314 257 39 4228
Fragments arranged by size:
4228 314 257 39
ApaLI G'TGCA_C
Cuts at: 3676 3676
Size: 4838
ApoI r'AATT_y
Cuts at: 1385 1842 4191 4447 1385
Size: 457 2349 256 1776
Fragments arranged by size:
2349 1776 457 256
AscI GG'CGCG_CC
Cuts at: 688 688
Size: 4838
AseI AT'TA_AT
Cuts at: 20 20
Size: 4838
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
AvaI C'yCGr_G
Cuts at: 131 164 727 1858 131
Size: 33 563 1131 3111
Fragments arranged by size:
3111 1131 563 33
BamHI G'GATC_C
Cuts at: 1863 1863
Size: 4838
BanI G'GyrC_C
Cuts at: 621 964 1264 1723 1854 2268 2516 2551 621
Size: 343 300 459 131 414 248 35 2908
Fragments arranged by size:
2908 459 414 343 300 248 131 35
BanII G_rGCy'C
Cuts at: 163 308 409 477 734 773 1852 2882 163
Size: 145 101 68 257 39 1079 1030 2119
Fragments arranged by size:
2119 1079 1030 257 145 101 68 39
BbeI G_GCGC'C
Cuts at: 2272 2520 2272
Size: 248 4590
BbsI GAAGACnn'nnnn_
Cuts at: 386 386
Size: 4838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 432 466 432
Size: 34 4804
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3792 4838 2736
Size: 1056 1046 2736
Fragments arranged by size:
2736 1056 1046
BclI T'GATC_A
Cuts at: 2358 2358
Size: 4838
BglI GCCn_nnn'nGGC
Cuts at: 208 523 802 827 208
Size: 315 279 25 4219
Fragments arranged by size:
4219 315 279 25
BglII A'GATC_T
Cuts at: 1103 2353 4390 1103
Size: 1250 2037 1551
Fragments arranged by size:
2037 1551 1250
BlpI GC'TnA_GC
Cuts at: 212 220 946 1079 1950 212
Size: 8 726 133 871 3100
Fragments arranged by size:
3100 871 726 133 8
Bme1580I G_kGCm'C
Cuts at: 163 477 626 734 773 783 1219 1267 1728
Size: 314 149 108 39 10 436 48 461
Cuts at: 1728 2463 2556 3680 163
Size: 735 93 1124 1321
Fragments arranged by size:
1321 1124 735 461 436 314 149 108
93 48 39 10
BmrI ACTGGGnnnn_n'
Cuts at: 530 1222 2050 2131 2466 4610 530
Size: 692 828 81 335 2144 758
Fragments arranged by size:
2144 828 758 692 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3205
Size: 4838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 676 940 1354 1375 1424 1690 393
Size: 283 264 414 21 49 266 3541
Fragments arranged by size:
3541 414 283 266 264 49 21
Bpu10I CC'TnA_GC
Cuts at: 617 617
Size: 4838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 622 1468 2014 2893 3607 3905 318
Size: 304 846 546 879 714 298 1251
Fragments arranged by size:
1251 879 846 714 546 304 298
BsaI GGTCTCn'nnnn_
Cuts at: 1105 1643 4542 1105
Size: 538 2899 1401
Fragments arranged by size:
2899 1401 538
BsaAI yAC'GTr
Cuts at: 2191 2191
Size: 4838
BsaBI GATnn'nnATC
Cuts at: 6 2352 4389 6
Size: 2346 2037 455
Fragments arranged by size:
2346 2037 455
BsaHI Gr'CG_yC
Cuts at: 575 2269 2517 3219 4715 575
Size: 1694 248 702 1496 698
Fragments arranged by size:
1694 1496 702 698 248
BsaWI w'CCGG_w
Cuts at: 240 2027 2548 3226 3637 3784 4058 240
Size: 1787 521 678 411 147 274 1020
Fragments arranged by size:
1787 1020 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 965 995 965
Size: 30 4808
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 1522 1525 1702 1705 1708 1840 202
Size: 1320 3 177 3 3 132 3200
Fragments arranged by size:
3200 1320 177 132 3 3 3
BseYI C'CCAG_C
Cuts at: 372 583 782 985 1074 1624 2263 3021 3686
Size: 211 199 203 89 550 639 758 665
Cuts at: 3686 4094 372
Size: 408 1116
Fragments arranged by size:
1116 758 665 639 550 408 211 203
199 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 664 1571 1600 527
Size: 137 907 29 3765
Fragments arranged by size:
3765 907 137 29
BsiEI CG_ry'CG
Cuts at: 104 122 203 2380 2426 3656 104
Size: 18 81 2177 46 1230 1286
Fragments arranged by size:
2177 1286 1230 81 46 18
BsiHKAI G_wGCw'C
Cuts at: 308 638 1852 2630 2820 3680 308
Size: 330 1214 778 190 860 1466
Fragments arranged by size:
1466 1214 860 778 330 190
BsmI GAATG_Cn'
Cuts at: 1569 1569
Size: 4838
BsmBI CGTCTCn'nnnn_
Cuts at: 582 582
Size: 4838
Bsp1286I G_dGCh'C
Cuts at: 163 308 409 477 626 638 734 773 783
Size: 145 101 68 149 12 96 39 10
Cuts at: 783 1219 1267 1728 1852 2463 2556 2630 2820
Size: 436 48 461 124 611 93 74 190
Cuts at: 2820 2882 3680 163
Size: 62 798 1321
Fragments arranged by size:
1321 798 611 461 436 190 149 145
124 101 96 93 74 68 62 48
39 12 10
BspEI T'CCGG_A
Cuts at: 240 240
Size: 4838
BspHI T'CATG_A
Cuts at: 3270 3270
Size: 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 555 686 690 1563 1622 1888 2102 2404 2785
Size: 131 4 873 59 266 214 302 381
Cuts at: 2785 555
Size: 2608
Fragments arranged by size:
2608 873 381 302 266 214 131 59
4
BsrBI CCG'CTC
Cuts at: 50 3130 3184 4668 4833 50
Size: 3080 54 1484 165 55
Fragments arranged by size:
3080 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2750 2750
Size: 4838
BsrFI r'CCGG_y
Cuts at: 198 692 817 1340 2836 3017 198
Size: 494 125 523 1496 181 2019
Fragments arranged by size:
2019 1496 523 494 181 125
BsrGI T'GTAC_A
Cuts at: 1022 1022
Size: 4838
BssHII G'CGCG_C
Cuts at: 688 688
Size: 4838
BssSI C'ACGA_G
Cuts at: 3109 3817 3109
Size: 708 4130
BstAPI GCAn_nnn'nTGC
Cuts at: 468 1070 2215 468
Size: 602 1145 3091
Fragments arranged by size:
3091 1145 602
BstBI TT'CG_AA
Cuts at: 3199 3199
Size: 4838
BstXI CCAn_nnnn'nTGG
Cuts at: 567 1332 1417 567
Size: 765 85 3988
Fragments arranged by size:
3988 765 85
BstYI r'GATC_y
Cuts at: 1 1103 1412 1863 2330 2353 2688 2934 3240
Size: 1102 309 451 467 23 335 246 306
Cuts at: 3240 3252 3338 4390 1
Size: 12 86 1052 449
Fragments arranged by size:
1102 1052 467 451 449 335 309 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 800 800
Size: 4838
BtgI C'CryG_G
Cuts at: 521 848 1416 521
Size: 327 568 3943
Fragments arranged by size:
3943 568 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2788 2969 3132 2788
Size: 181 163 4494
Fragments arranged by size:
4494 181 163
BtsI GCAGTG_nn'
Cuts at: 349 787 837 1735 2081 349
Size: 438 50 898 346 3106
Fragments arranged by size:
3106 898 438 346 50
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4838
DrdI GACnn_nn'nnGTC
Cuts at: 2544 3888 2544
Size: 1344 3494
EaeI y'GGCC_r
Cuts at: 119 200 1040 1457 2423 2597 2988 3015 4360
Size: 81 840 417 966 174 391 27 1345
Cuts at: 4360 119
Size: 597
Fragments arranged by size:
1345 966 840 597 417 391 174 81
27
EagI C'GGCC_G
Cuts at: 119 200 2423 119
Size: 81 2223 2534
Fragments arranged by size:
2534 2223 81
EarI CTCTTCn'nnn_
Cuts at: 1770 1817 2861 3071 1770
Size: 47 1044 210 3537
Fragments arranged by size:
3537 1044 210 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3046 3788 3934 4648 3046
Size: 742 146 714 3236
Fragments arranged by size:
3236 742 714 146
EcoICRI GAG'CTC
Cuts at: 306 1850 306
Size: 1544 3294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 676 940 1294 1354 1375 1424 1690 1751
Size: 283 264 354 60 21 49 266 61
Cuts at: 1751 1797 2663 3095 3448 4110 393
Size: 46 866 432 353 662 1121
Fragments arranged by size:
1121 866 662 432 354 353 283 266
264 61 60 49 46 21
EcoNI CCTnn'n_nnAGG
Cuts at: 682 1001 1132 1357 682
Size: 319 131 225 4163
Fragments arranged by size:
4163 319 225 131
EcoO109I rG'GnC_Cy
Cuts at: 159 160 413 750 769 1125 1552 1641 1977
Size: 1 253 337 19 356 427 89 336
Cuts at: 1977 159
Size: 3020
Fragments arranged by size:
3020 427 356 337 336 253 89 19
1
EcoRI G'AATT_C
Cuts at: 1385 1842 1385
Size: 457 4381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4838
FseI GG_CCGG'CC
Cuts at: 202 202
Size: 4838
FspI TGC'GCA
Cuts at: 2619 2619
Size: 4838
HaeII r_GCGC'y
Cuts at: 856 1323 2272 2520 3750 4521 856
Size: 467 949 248 1230 771 1173
Fragments arranged by size:
1230 1173 949 771 467 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 965 997 2680 2710 2712 2742 965
Size: 32 1683 30 2 30 3061
Fragments arranged by size:
3061 1683 32 30 30 2
HincII GTy'rAC
Cuts at: 1877 1877
Size: 4838
KasI G'GCGC_C
Cuts at: 2268 2516 2268
Size: 248 4590
KpnI G_GTAC'C
Cuts at: 1858 1858
Size: 4838
MluI A'CGCG_T
Cuts at: 4271 4271
Size: 4838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 164 3597 3781 164
Size: 3433 184 1221
Fragments arranged by size:
3433 1221 184
MscI TGG'CCA
Cuts at: 1042 1459 2599 1042
Size: 417 1140 3281
Fragments arranged by size:
3281 1140 417
MslI CAynn'nnrTG
Cuts at: 565 1681 2827 2954 565
Size: 1116 1146 127 2449
Fragments arranged by size:
2449 1146 1116 127
MspA1I CmG'CkG
Cuts at: 216 523 1078 1497 1949 2263 2623 3407 3652
Size: 307 555 419 452 314 360 784 245
Cuts at: 3652 216
Size: 1402
Fragments arranged by size:
1402 784 555 452 419 360 314 307
245
NaeI GCC'GGC
Cuts at: 200 694 1342 3019 200
Size: 494 648 1677 2019
Fragments arranged by size:
2019 1677 648 494
NarI GG'CG_CC
Cuts at: 2269 2517 2269
Size: 248 4590
NcoI C'CATG_G
Cuts at: 848 1416 848
Size: 568 4270
NgoMIV G'CCGG_C
Cuts at: 198 692 1340 3017 198
Size: 494 648 1677 2019
Fragments arranged by size:
2019 1677 648 494
NspI r_CATG'y
Cuts at: 1891 2391 3994 1891
Size: 500 1603 2735
Fragments arranged by size:
2735 1603 500
PasI CC'CwG_GG
Cuts at: 626 626
Size: 4838
PciI A'CATG_T
Cuts at: 3990 3990
Size: 4838
PfoI T'CCnGG_A
Cuts at: 247 409 247
Size: 162 4676
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 4806
PpuMI rG'GwC_Cy
Cuts at: 413 1552 1641 413
Size: 1139 89 3610
Fragments arranged by size:
3610 1139 89
PspOMI G'GGCC_C
Cuts at: 159 473 730 769 159
Size: 314 257 39 4228
Fragments arranged by size:
4228 314 257 39
PspXI vC'TCGA_Gb
Cuts at: 164 164
Size: 4838
PstI C_TGCA'G
Cuts at: 356 402 683 980 1307 1379 1406 1885 2570
Size: 46 281 297 327 72 27 479 685
Cuts at: 2570 356
Size: 2624
Fragments arranged by size:
2624 685 479 327 297 281 72 46
27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4838
PvuII CAG'CTG
Cuts at: 216 1078 1497 2263 2623 216
Size: 862 419 766 360 2431
Fragments arranged by size:
2431 862 766 419 360
RsrII CG'GwC_CG
Cuts at: 3033 3033
Size: 4838
SacI G_AGCT'C
Cuts at: 308 1852 308
Size: 1544 3294
SacII CC_GC'GG
Cuts at: 524 524
Size: 4838
SalI G'TCGA_C
Cuts at: 1875 1875
Size: 4838
SapI GCTCTTCn'nnn_
Cuts at: 2861 3071 2861
Size: 210 4628
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4838
ScaI AGT'ACT
Cuts at: 4355 4355
Size: 4838
SfcI C'TryA_G
Cuts at: 33 352 398 679 976 1303 1375 1402 1881
Size: 319 46 281 297 327 72 27 479
Cuts at: 1881 2566 3534 3725 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 685 479 327 319 297 281
191 72 46 27
SfoI GGC'GCC
Cuts at: 2270 2518 2270
Size: 248 4590
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
SmaI CCC'GGG
Cuts at: 1860 1860
Size: 4838
SmlI C'TyrA_G
Cuts at: 164 333 637 1483 1993 2908 3622 3884 164
Size: 169 304 846 510 915 714 262 1118
Fragments arranged by size:
1118 915 846 714 510 304 262 169
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4838
SphI G_CATG'C
Cuts at: 1891 2391 1891
Size: 500 4338
StuI AGG'CCT
Cuts at: 1279 1279
Size: 4838
StyI C'CwwG_G
Cuts at: 848 1416 1454 1787 1972 2326 848
Size: 568 38 333 185 354 3360
Fragments arranged by size:
3360 568 354 333 185 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1129 2421 2554 3013 3222 1129
Size: 1292 133 459 209 2745
Fragments arranged by size:
2745 1292 459 209 133
TatI w'GTAC_w
Cuts at: 1022 4353 1022
Size: 3331 1507
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4355 4387 4355
Size: 32 4806
Tth111I GACn'n_nGTC
Cuts at: 2635 2635
Size: 4838
XbaI T'CTAG_A
Cuts at: 1869 1869
Size: 4838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 846 1632 2333 846
Size: 786 701 3351
Fragments arranged by size:
3351 786 701
XhoI C'TCGA_G
Cuts at: 164 164
Size: 4838
XmaI C'CCGG_G
Cuts at: 1858 1858
Size: 4838
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AloI AvrII BaeI
BbvCI BfrBI BmgBI BmtI BsiWI BstEII BstZ17I ClaI
CspCI DraIII EcoRV FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI