Restriction map for
pF1KB3129
(Circular) (Six-base) MAPSORT of: pF1KB3129.seq Check: 7284 from: 1 to: 3830
pF1KB3129 3830 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 668 668
Size: 3830
AccI GT'mk_AC
Cuts at: 868 868
Size: 3830
Acc65I G'GTAC_C
Cuts at: 846 846
Size: 3830
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 778 1655 2087 2440 3102 504
Size: 274 877 432 353 662 1232
Fragments arranged by size:
1232 877 662 432 353 274
AflIII A'CryG_T
Cuts at: 1182 2982 3263 1182
Size: 1800 281 1749
Fragments arranged by size:
1800 1749 281
AleI CACnn'nnGTG
Cuts at: 505 505
Size: 3830
AlwNI CAG_nnn'CTG
Cuts at: 1135 2573 1135
Size: 1438 2392
ApaLI G'TGCA_C
Cuts at: 2668 2668
Size: 3830
ApoI r'AATT_y
Cuts at: 834 3183 3439 834
Size: 2349 256 1225
Fragments arranged by size:
2349 1225 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3830
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 225 265 293 850 225
Size: 40 28 557 3205
Fragments arranged by size:
3205 557 40 28
BamHI G'GATC_C
Cuts at: 671 855 671
Size: 184 3646
BanI G'GyrC_C
Cuts at: 214 343 372 656 805 846 1260 1508 1543
Size: 129 29 284 149 41 414 248 35
Cuts at: 1543 214
Size: 2501
Fragments arranged by size:
2501 414 284 248 149 129 41 35
29
BanII G_rGCy'C
Cuts at: 518 844 1874 518
Size: 326 1030 2474
Fragments arranged by size:
2474 1030 326
BbeI G_GCGC'C
Cuts at: 218 347 1264 1512 218
Size: 129 917 248 2536
Fragments arranged by size:
2536 917 248 129
BbsI GAAGACnn'nnnn_
Cuts at: 766 766
Size: 3830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 181 215 181
Size: 34 3796
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2784 3830 1728
Size: 1056 1046 1728
Fragments arranged by size:
1728 1056 1046
BclI T'GATC_A
Cuts at: 1350 1350
Size: 3830
BglI GCCn_nnn'nGGC
Cuts at: 407 407
Size: 3830
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 364 942 364
Size: 578 3252
Bme1580I G_kGCm'C
Cuts at: 272 1455 1548 2672 272
Size: 1183 93 1124 1430
Fragments arranged by size:
1430 1183 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 546 1042 1123 1458 3602 546
Size: 496 81 335 2144 774
Fragments arranged by size:
2144 774 496 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1885 2599 2897 1006
Size: 879 714 298 1939
Fragments arranged by size:
1939 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3534 3534
Size: 3830
BsaAI yAC'GTr
Cuts at: 1183 1183
Size: 3830
BsaBI GATnn'nnATC
Cuts at: 6 1344 3381 6
Size: 1338 2037 455
Fragments arranged by size:
2037 1338 455
BsaHI Gr'CG_yC
Cuts at: 215 344 472 1261 1509 2211 3707 215
Size: 129 128 789 248 702 1496 338
Fragments arranged by size:
1496 789 702 338 248 129 128
BsaWI w'CCGG_w
Cuts at: 1019 1540 2218 2629 2776 3050 1019
Size: 521 678 411 147 274 1799
Fragments arranged by size:
1799 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 403 433 403
Size: 30 3800
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 246 526 246
Size: 280 3550
BseYI C'CCAG_C
Cuts at: 118 260 689 718 1255 2013 2678 3086 118
Size: 142 429 29 537 758 665 408 862
Fragments arranged by size:
862 758 665 537 429 408 142 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 646 448
Size: 198 3632
BsiEI CG_ry'CG
Cuts at: 104 305 1372 1418 2648 104
Size: 201 1067 46 1230 1286
Fragments arranged by size:
1286 1230 1067 201 46
BsiHKAI G_wGCw'C
Cuts at: 518 844 1622 1812 2672 518
Size: 326 778 190 860 1676
Fragments arranged by size:
1676 860 778 326 190
Bsp1286I G_dGCh'C
Cuts at: 272 518 844 1455 1548 1622 1812 1874 2672
Size: 246 326 611 93 74 190 62 798
Cuts at: 2672 272
Size: 1430
Fragments arranged by size:
1430 798 611 326 246 190 93 74
62
BspHI T'CATG_A
Cuts at: 2262 2262
Size: 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 668 880 1094 1396 1777 668
Size: 212 214 302 381 2721
Fragments arranged by size:
2721 381 302 214 212
BsrBI CCG'CTC
Cuts at: 50 2122 2176 3660 3825 50
Size: 2072 54 1484 165 55
Fragments arranged by size:
2072 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1742 1742
Size: 3830
BsrFI r'CCGG_y
Cuts at: 406 653 1828 2009 406
Size: 247 1175 181 2227
Fragments arranged by size:
2227 1175 247 181
BssSI C'ACGA_G
Cuts at: 2101 2809 2101
Size: 708 3122
BstAPI GCAn_nnn'nTGC
Cuts at: 1207 1207
Size: 3830
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstXI CCAn_nnnn'nTGG
Cuts at: 507 507
Size: 3830
BstYI r'GATC_y
Cuts at: 1 671 855 1322 1345 1680 1926 2232 2244
Size: 670 184 467 23 335 246 306 12
Cuts at: 2244 2330 3382 1
Size: 86 1052 449
Fragments arranged by size:
1052 670 467 449 335 306 246 184
86 23 12
BtgI C'CryG_G
Cuts at: 107 350 500 107
Size: 243 150 3437
Fragments arranged by size:
3437 243 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 609 1780 1961 2124 609
Size: 1171 181 163 2315
Fragments arranged by size:
2315 1171 181 163
BtsI GCAGTG_nn'
Cuts at: 1073 1073
Size: 3830
DraI TTT'AAA
Cuts at: 829 829
Size: 3830
DrdI GACnn_nn'nnGTC
Cuts at: 1536 2880 1536
Size: 1344 2486
EaeI y'GGCC_r
Cuts at: 1415 1589 1980 2007 3352 1415
Size: 174 391 27 1345 1893
Fragments arranged by size:
1893 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1415 1415
Size: 3830
EarI CTCTTCn'nnn_
Cuts at: 523 1853 2063 523
Size: 1330 210 2290
Fragments arranged by size:
2290 1330 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 388 2038 2780 2926 3640 388
Size: 1650 742 146 714 578
Fragments arranged by size:
1650 742 714 578 146
EcoICRI GAG'CTC
Cuts at: 516 842 516
Size: 326 3504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 778 1655 2087 2440 3102 504
Size: 274 877 432 353 662 1232
Fragments arranged by size:
1232 877 662 432 353 274
EcoNI CCTnn'n_nnAGG
Cuts at: 400 400
Size: 3830
EcoO109I rG'GnC_Cy
Cuts at: 297 311 355 969 297
Size: 14 44 614 3158
Fragments arranged by size:
3158 614 44 14
EcoRI G'AATT_C
Cuts at: 834 834
Size: 3830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3830
FspI TGC'GCA
Cuts at: 1611 1611
Size: 3830
HaeII r_GCGC'y
Cuts at: 218 347 1264 1512 2742 3513 218
Size: 129 917 248 1230 771 535
Fragments arranged by size:
1230 917 771 535 248 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 779 811 1672 1702 1704 1734 403
Size: 32 344 32 861 30 2 30 2499
Fragments arranged by size:
2499 861 344 32 32 30 30 2
HincII GTy'rAC
Cuts at: 594 869 594
Size: 275 3555
KasI G'GCGC_C
Cuts at: 214 343 1260 1508 214
Size: 129 917 248 2536
Fragments arranged by size:
2536 917 248 129
KpnI G_GTAC'C
Cuts at: 850 850
Size: 3830
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2589 2773 2589
Size: 184 3646
MscI TGG'CCA
Cuts at: 1591 1591
Size: 3830
MslI CAynn'nnrTG
Cuts at: 505 580 1819 1946 505
Size: 75 1239 127 2389
Fragments arranged by size:
2389 1239 127 75
MspA1I CmG'CkG
Cuts at: 321 352 462 941 1255 1615 2399 2644 321
Size: 31 110 479 314 360 784 245 1507
Fragments arranged by size:
1507 784 479 360 314 245 110 31
NaeI GCC'GGC
Cuts at: 408 2011 408
Size: 1603 2227
NarI GG'CG_CC
Cuts at: 215 344 1261 1509 215
Size: 129 917 248 2536
Fragments arranged by size:
2536 917 248 129
NcoI C'CATG_G
Cuts at: 107 107
Size: 3830
NgoMIV G'CCGG_C
Cuts at: 406 2009 406
Size: 1603 2227
NspI r_CATG'y
Cuts at: 726 883 1383 2986 726
Size: 157 500 1603 1570
Fragments arranged by size:
1603 1570 500 157
PasI CC'CwG_GG
Cuts at: 801 801
Size: 3830
PciI A'CATG_T
Cuts at: 2982 2982
Size: 3830
PfoI T'CCnGG_A
Cuts at: 674 674
Size: 3830
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2276 2308 2276
Size: 32 3798
PpuMI rG'GwC_Cy
Cuts at: 297 311 297
Size: 14 3816
PsiI TTA'TAA
Cuts at: 170 170
Size: 3830
PstI C_TGCA'G
Cuts at: 326 877 1562 326
Size: 551 685 2594
Fragments arranged by size:
2594 685 551
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 462 1255 1615 462
Size: 793 360 2677
Fragments arranged by size:
2677 793 360
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 518 844 518
Size: 326 3504
SacII CC_GC'GG
Cuts at: 353 353
Size: 3830
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SanDI GG'GwC_CC
Cuts at: 297 297
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 523 1853 2063 523
Size: 1330 210 2290
Fragments arranged by size:
2290 1330 210
SbfI CC_TGCA'GG
Cuts at: 877 877
Size: 3830
ScaI AGT'ACT
Cuts at: 3347 3347
Size: 3830
SfcI C'TryA_G
Cuts at: 33 322 873 1558 2526 2717 33
Size: 289 551 685 968 191 1146
Fragments arranged by size:
1146 968 685 551 289 191
SfoI GGC'GCC
Cuts at: 216 345 1262 1510 216
Size: 129 917 248 2536
Fragments arranged by size:
2536 917 248 129
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SmaI CCC'GGG
Cuts at: 227 267 295 852 227
Size: 40 28 557 3205
Fragments arranged by size:
3205 557 40 28
SmlI C'TyrA_G
Cuts at: 985 1900 2614 2876 985
Size: 915 714 262 1939
Fragments arranged by size:
1939 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3830
SphI G_CATG'C
Cuts at: 726 883 1383 726
Size: 157 500 3173
Fragments arranged by size:
3173 500 157
SrfI GCCC'GGGC
Cuts at: 267 267
Size: 3830
StyI C'CwwG_G
Cuts at: 107 964 1318 107
Size: 857 354 2619
Fragments arranged by size:
2619 857 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 793 1413 1546 2005 2214 793
Size: 620 133 459 209 2409
Fragments arranged by size:
2409 620 459 209 133
TatI w'GTAC_w
Cuts at: 158 3345 158
Size: 3187 643
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 264 296 368 400 3347 3379 264
Size: 32 72 32 2947 32 715
Fragments arranged by size:
2947 715 72 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 301 1627 301
Size: 1326 2504
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1325 1325
Size: 3830
XmaI C'CCGG_G
Cuts at: 225 265 293 850 225
Size: 40 28 557 3205
Fragments arranged by size:
3205 557 40 28
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI
Bpu10I BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PspOMI PspXI PsrI SexAI
SfiI SgrAI SnaBI SspI StuI SwaI XhoI XmnI
ZraI