Restriction map for
pF1KB3126
(Circular) (Six-base) MAPSORT of: pF1KB3126.seq Check: 7092 from: 1 to: 6017
pF1KB3126 6017 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 437 437
Size: 6017
AatII G_ACGT'C
Cuts at: 2138 2138
Size: 6017
AccI GT'mk_AC
Cuts at: 528 3055 528
Size: 2527 3490
Acc65I G'GTAC_C
Cuts at: 3033 3033
Size: 6017
AclI AA'CG_TT
Cuts at: 5857 5857
Size: 6017
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 2564 3842 4274 4627 5289 1457
Size: 1107 1278 432 353 662 2185
Fragments arranged by size:
2185 1278 1107 662 432 353
AflIII A'CryG_T
Cuts at: 3369 5169 5450 3369
Size: 1800 281 3936
Fragments arranged by size:
3936 1800 281
AleI CACnn'nnGTG
Cuts at: 1717 1717
Size: 6017
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1664 1988 2113 2188 1664
Size: 324 125 75 5493
Fragments arranged by size:
5493 324 125 75
AlwNI CAG_nnn'CTG
Cuts at: 1090 2879 3322 4760 1090
Size: 1789 443 1438 2347
Fragments arranged by size:
2347 1789 1438 443
ApaLI G'TGCA_C
Cuts at: 619 4855 619
Size: 4236 1781
ApoI r'AATT_y
Cuts at: 908 2579 3021 5370 5626 908
Size: 1671 442 2349 256 1299
Fragments arranged by size:
2349 1671 1299 442 256
AseI AT'TA_AT
Cuts at: 20 1070 20
Size: 1050 4967
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6017
AvaI C'yCGr_G
Cuts at: 3037 3037
Size: 6017
BamHI G'GATC_C
Cuts at: 159 3042 159
Size: 2883 3134
BanI G'GyrC_C
Cuts at: 319 794 1243 2941 3033 3447 3695 3730 319
Size: 475 449 1698 92 414 248 35 2606
Fragments arranged by size:
2606 1698 475 449 414 248 92 35
BanII G_rGCy'C
Cuts at: 1520 2861 3031 4061 1520
Size: 1341 170 1030 3476
Fragments arranged by size:
3476 1341 1030 170
BbeI G_GCGC'C
Cuts at: 798 3451 3699 798
Size: 2653 248 3116
Fragments arranged by size:
3116 2653 248
BbsI GAAGACnn'nnnn_
Cuts at: 1035 2800 1035
Size: 1765 4252
BbvCI CC'TCA_GC
Cuts at: 733 1912 733
Size: 1179 4838
BciVI GTATCCnnnnn_n'
Cuts at: 2627 3915 4971 6017 2627
Size: 1288 1056 1046 2627
Fragments arranged by size:
2627 1288 1056 1046
BclI T'GATC_A
Cuts at: 1877 3537 1877
Size: 1660 4357
BfrBI ATG'CAT
Cuts at: 847 847
Size: 6017
BglI GCCn_nnn'nGGC
Cuts at: 2128 2128
Size: 6017
BglII A'GATC_T
Cuts at: 3532 5569 3532
Size: 2037 3980
BlpI GC'TnA_GC
Cuts at: 3129 3129
Size: 6017
Bme1580I G_kGCm'C
Cuts at: 623 1297 3642 3735 4859 623
Size: 674 2345 93 1124 1781
Fragments arranged by size:
2345 1781 1124 674 93
BmgBI CAC'GTC
Cuts at: 1680 1680
Size: 6017
BmrI ACTGGGnnnn_n'
Cuts at: 2610 2782 3229 3310 3645 5789 2610
Size: 172 447 81 335 2144 2838
Fragments arranged by size:
2838 2144 447 335 172 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2681 4384 2681
Size: 1703 4314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 1274 1610 2245 2766 917
Size: 357 336 635 521 4168
Fragments arranged by size:
4168 635 521 357 336
Bpu10I CC'TnA_GC
Cuts at: 733 1912 1930 2144 733
Size: 1179 18 214 4606
Fragments arranged by size:
4606 1179 214 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1996 3193 4072 4786 5084 1996
Size: 1197 879 714 298 2929
Fragments arranged by size:
2929 1197 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2703 5721 2703
Size: 3018 2999
BsaAI yAC'GTr
Cuts at: 3370 3370
Size: 6017
BsaBI GATnn'nnATC
Cuts at: 6 3531 5568 6
Size: 3525 2037 455
Fragments arranged by size:
3525 2037 455
BsaHI Gr'CG_yC
Cuts at: 795 818 1858 2135 3448 3696 4398 5894 795
Size: 23 1040 277 1313 248 702 1496 918
Fragments arranged by size:
1496 1313 1040 918 702 277 248 23
BsaWI w'CCGG_w
Cuts at: 768 3206 3727 4405 4816 4963 5237 768
Size: 2438 521 678 411 147 274 1548
Fragments arranged by size:
2438 1548 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2140 2170 2140
Size: 30 5987
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1301 1301
Size: 6017
BseYI C'CCAG_C
Cuts at: 669 1390 1519 1918 2125 2287 3442 4200 4865
Size: 721 129 399 207 162 1155 758 665
Cuts at: 4865 5273 669
Size: 408 1413
Fragments arranged by size:
1413 1155 758 721 665 408 399 207
162 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 1204 1741 2158 459
Size: 745 537 417 4318
Fragments arranged by size:
4318 745 537 417
BsiEI CG_ry'CG
Cuts at: 104 3559 3605 4835 104
Size: 3455 46 1230 1286
Fragments arranged by size:
3455 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 623 2192 2861 3031 3809 3999 4859 623
Size: 1569 669 170 778 190 860 1781
Fragments arranged by size:
1781 1569 860 778 669 190 170
BsmI GAATG_Cn'
Cuts at: 2070 2070
Size: 6017
BsmBI CGTCTCn'nnnn_
Cuts at: 1670 1670
Size: 6017
Bsp1286I G_dGCh'C
Cuts at: 623 1297 1520 2192 2861 3031 3642 3735 3809
Size: 674 223 672 669 170 611 93 74
Cuts at: 3809 3999 4061 4859 623
Size: 190 62 798 1781
Fragments arranged by size:
1781 798 674 672 669 611 223 190
170 93 74 62
BspEI T'CCGG_A
Cuts at: 768 768
Size: 6017
BspHI T'CATG_A
Cuts at: 4449 4449
Size: 6017
BspMI ACCTGCnnnn'nnnn_
Cuts at: 437 2445 3067 3281 3583 3964 437
Size: 2008 622 214 302 381 2490
Fragments arranged by size:
2490 2008 622 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4309 4363 5847 6012 50
Size: 4259 54 1484 165 55
Fragments arranged by size:
4259 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3929 3929
Size: 6017
BsrFI r'CCGG_y
Cuts at: 662 4015 4196 662
Size: 3353 181 2483
Fragments arranged by size:
3353 2483 181
BssSI C'ACGA_G
Cuts at: 4288 4996 4288
Size: 708 5309
BstAPI GCAn_nnn'nTGC
Cuts at: 3394 3394
Size: 6017
BstBI TT'CG_AA
Cuts at: 4378 4378
Size: 6017
BstXI CCAn_nnnn'nTGG
Cuts at: 501 726 2307 2980 501
Size: 225 1581 673 3538
Fragments arranged by size:
3538 1581 673 225
BstYI r'GATC_y
Cuts at: 1 159 470 1788 2337 3042 3509 3532 3867
Size: 158 311 1318 549 705 467 23 335
Cuts at: 3867 4113 4419 4431 4517 5569 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1318 1052 705 549 467 449 335 311
306 246 158 86 23 12
BstZ17I GTA'TAC
Cuts at: 529 529
Size: 6017
Bsu36I CC'TnA_GG
Cuts at: 353 353
Size: 6017
BtgI C'CryG_G
Cuts at: 107 303 806 1409 107
Size: 196 503 603 4715
Fragments arranged by size:
4715 603 503 196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3967 4148 4311 3967
Size: 181 163 5673
Fragments arranged by size:
5673 181 163
BtsI GCAGTG_nn'
Cuts at: 214 1712 1880 2170 2947 3260 214
Size: 1498 168 290 777 313 2971
Fragments arranged by size:
2971 1498 777 313 290 168
DraI TTT'AAA
Cuts at: 3016 3016
Size: 6017
DrdI GACnn_nn'nnGTC
Cuts at: 505 3723 5067 505
Size: 3218 1344 1455
Fragments arranged by size:
3218 1455 1344
EaeI y'GGCC_r
Cuts at: 1694 2120 2441 3602 3776 4167 4194 5539 1694
Size: 426 321 1161 174 391 27 1345 2172
Fragments arranged by size:
2172 1345 1161 426 391 321 174 27
EagI C'GGCC_G
Cuts at: 3602 3602
Size: 6017
EarI CTCTTCn'nnn_
Cuts at: 959 1574 2321 4040 4250 959
Size: 615 747 1719 210 2726
Fragments arranged by size:
2726 1719 747 615 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 685 1274 4225 4967 5113 5827 685
Size: 589 2951 742 146 714 875
Fragments arranged by size:
2951 875 742 714 589 146
EcoICRI GAG'CTC
Cuts at: 2859 3029 2859
Size: 170 5847
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 1274 1457 1610 2245 2564 2766 3842 4274
Size: 357 183 153 635 319 202 1076 432
Cuts at: 4274 4627 5289 917
Size: 353 662 1645
Fragments arranged by size:
1645 1076 662 635 432 357 353 319
202 183 153
EcoNI CCTnn'n_nnAGG
Cuts at: 2838 2999 2838
Size: 161 5856
EcoO109I rG'GnC_Cy
Cuts at: 1431 2730 2992 3156 1431
Size: 1299 262 164 4292
Fragments arranged by size:
4292 1299 262 164
EcoRI G'AATT_C
Cuts at: 3021 3021
Size: 6017
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4036 4036
Size: 6017
FspI TGC'GCA
Cuts at: 3798 3798
Size: 6017
HaeII r_GCGC'y
Cuts at: 798 1707 3451 3699 4929 5700 798
Size: 909 1744 248 1230 771 1115
Fragments arranged by size:
1744 1230 1115 909 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1179 1211 1405 1437 1830 1862 2140 2172 2185
Size: 32 194 32 393 32 278 32 13
Cuts at: 2185 2217 2485 2517 2787 2819 3859 3889 3891
Size: 32 268 32 270 32 1040 30 2
Cuts at: 3891 3921 1179
Size: 30 3275
Fragments arranged by size:
3275 1040 393 278 270 268 194 32
32 32 32 32 32 32 30 30
13 2
HincII GTy'rAC
Cuts at: 3056 3056
Size: 6017
KasI G'GCGC_C
Cuts at: 794 3447 3695 794
Size: 2653 248 3116
Fragments arranged by size:
3116 2653 248
KpnI G_GTAC'C
Cuts at: 3037 3037
Size: 6017
MfeI C'AATT_G
Cuts at: 2759 2759
Size: 6017
MluI A'CGCG_T
Cuts at: 5450 5450
Size: 6017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 875 4776 4960 875
Size: 3901 184 1932
Fragments arranged by size:
3901 1932 184
MscI TGG'CCA
Cuts at: 2443 3778 2443
Size: 1335 4682
MslI CAynn'nnrTG
Cuts at: 431 1646 1717 2978 4006 4133 431
Size: 1215 71 1261 1028 127 2315
Fragments arranged by size:
2315 1261 1215 1028 127 71
MspA1I CmG'CkG
Cuts at: 669 883 2125 2287 2450 2538 2562 2888 3128
Size: 214 1242 162 163 88 24 326 240
Cuts at: 3128 3442 3802 4586 4831 669
Size: 314 360 784 245 1855
Fragments arranged by size:
1855 1242 784 360 326 314 245 240
214 163 162 88 24
NaeI GCC'GGC
Cuts at: 4198 4198
Size: 6017
NarI GG'CG_CC
Cuts at: 795 3448 3696 795
Size: 2653 248 3116
Fragments arranged by size:
3116 2653 248
NcoI C'CATG_G
Cuts at: 107 303 1409 107
Size: 196 1106 4715
Fragments arranged by size:
4715 1106 196
NdeI CA'TA_TG
Cuts at: 2484 2484
Size: 6017
NgoMIV G'CCGG_C
Cuts at: 4196 4196
Size: 6017
NsiI A_TGCA'T
Cuts at: 849 849
Size: 6017
NspI r_CATG'y
Cuts at: 436 1269 1645 3070 3570 5173 436
Size: 833 376 1425 500 1603 1280
Fragments arranged by size:
1603 1425 1280 833 500 376
PciI A'CATG_T
Cuts at: 5169 5169
Size: 6017
PflMI CCAn_nnn'nTGG
Cuts at: 226 304 444 1333 2450 226
Size: 78 140 889 1117 3793
Fragments arranged by size:
3793 1117 889 140 78
PfoI T'CCnGG_A
Cuts at: 606 1368 2222 606
Size: 762 854 4401
Fragments arranged by size:
4401 854 762
PmeI GTTT'AAAC
Cuts at: 3016 3016
Size: 6017
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2415 2447 4463 4495 2415
Size: 32 2016 32 3937
Fragments arranged by size:
3937 2016 32 32
PpuMI rG'GwC_Cy
Cuts at: 1431 2992 1431
Size: 1561 4456
PstI C_TGCA'G
Cuts at: 713 2561 3064 3749 713
Size: 1848 503 685 2981
Fragments arranged by size:
2981 1848 685 503
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6017
PvuII CAG'CTG
Cuts at: 669 883 2287 2450 2538 2562 3442 3802 669
Size: 214 1404 163 88 24 880 360 2884
Fragments arranged by size:
2884 1404 880 360 214 163 88 24
RsrII CG'GwC_CG
Cuts at: 4212 4212
Size: 6017
SacI G_AGCT'C
Cuts at: 2861 3031 2861
Size: 170 5847
SalI G'TCGA_C
Cuts at: 3054 3054
Size: 6017
SapI GCTCTTCn'nnn_
Cuts at: 2321 4040 4250 2321
Size: 1719 210 4088
Fragments arranged by size:
4088 1719 210
SbfI CC_TGCA'GG
Cuts at: 3064 3064
Size: 6017
ScaI AGT'ACT
Cuts at: 2250 5534 2250
Size: 3284 2733
SexAI A'CCwGG_T
Cuts at: 315 315
Size: 6017
SfcI C'TryA_G
Cuts at: 33 709 2557 3060 3745 4713 4904 33
Size: 676 1848 503 685 968 191 1146
Fragments arranged by size:
1848 1146 968 685 676 503 191
SfoI GGC'GCC
Cuts at: 796 3449 3697 796
Size: 2653 248 3116
Fragments arranged by size:
3116 2653 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6017
SmaI CCC'GGG
Cuts at: 3039 3039
Size: 6017
SmlI C'TyrA_G
Cuts at: 2011 3172 4087 4801 5063 2011
Size: 1161 915 714 262 2965
Fragments arranged by size:
2965 1161 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1129 67
Size: 1062 4955
SphI G_CATG'C
Cuts at: 436 1645 3070 3570 436
Size: 1209 1425 500 2883
Fragments arranged by size:
2883 1425 1209 500
SspI AAT'ATT
Cuts at: 182 245 1074 182
Size: 63 829 5125
Fragments arranged by size:
5125 829 63
StuI AGG'CCT
Cuts at: 138 138
Size: 6017
StyI C'CwwG_G
Cuts at: 107 303 494 719 1409 3151 3505 107
Size: 196 191 225 690 1742 354 2619
Fragments arranged by size:
2619 1742 690 354 225 196 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3600 3733 4192 4401 3600
Size: 133 459 209 5216
Fragments arranged by size:
5216 459 209 133
TatI w'GTAC_w
Cuts at: 1303 2248 5532 1303
Size: 945 3284 1788
Fragments arranged by size:
3284 1788 945
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 602 634 5534 5566 602
Size: 32 4900 32 1053
Fragments arranged by size:
4900 1053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1741 2875 3814 1741
Size: 1134 939 3944
Fragments arranged by size:
3944 1134 939
XbaI T'CTAG_A
Cuts at: 1611 2433 2997 3048 1611
Size: 822 564 51 4580
Fragments arranged by size:
4580 822 564 51
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 501 2597 2679 3512 501
Size: 2096 82 833 3006
Fragments arranged by size:
3006 2096 833 82
XmaI C'CCGG_G
Cuts at: 3037 3037
Size: 6017
XmnI GAAnn'nnTTC
Cuts at: 197 197
Size: 6017
ZraI GAC'GTC
Cuts at: 2136 2136
Size: 6017
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AloI ApaI AscI AvrII
BaeI BcgI BmtI BsiWI BsrGI BssHII BstEII ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NheI
NotI NruI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI XhoI