Restriction map for
pF1KB3115
(Circular) (Six-base) MAPSORT of: pF1KB3115.seq Check: 9986 from: 1 to: 4382
pF1KB3115 4382 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1270 2207 2639 3386 3654 712
Size: 558 937 432 747 268 1440
Fragments arranged by size:
1440 937 747 558 432 268
AflII C'TTAA_G
Cuts at: 478 478
Size: 4382
AflIII A'CryG_T
Cuts at: 1734 2838 3815 1734
Size: 1104 977 2301
Fragments arranged by size:
2301 1104 977
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 459 1687 3254 459
Size: 1228 1567 1587
Fragments arranged by size:
1587 1567 1228
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 1386 3735 3991 1386
Size: 2349 256 1777
Fragments arranged by size:
2349 1777 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 244 1402 244
Size: 1158 3224
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 1398 1812 2060 2095 1398
Size: 414 248 35 3685
Fragments arranged by size:
3685 414 248 35
BanII G_rGCy'C
Cuts at: 252 711 1336 1396 2426 252
Size: 459 625 60 1030 2208
Fragments arranged by size:
2208 1030 625 459 60
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 188 1236 1902 188
Size: 1048 666 2668
Fragments arranged by size:
2668 1048 666
BfrBI ATG'CAT
Cuts at: 883 883
Size: 4382
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 2007 2100 3156 2007
Size: 93 1056 3233
Fragments arranged by size:
3233 1056 93
BmgBI CAC'GTC
Cuts at: 597 597
Size: 4382
BmrI ACTGGGnnnn_n'
Cuts at: 308 695 1594 1675 2010 4154 308
Size: 387 899 81 335 2144 536
Fragments arranged by size:
2144 899 536 387 335 81
BmtI G_CTAG'C
Cuts at: 562 562
Size: 4382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 142
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 1558 2437 2929 3227 143
Size: 1415 879 492 298 1298
Fragments arranged by size:
1415 1298 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4086 4086
Size: 4382
BsaAI yAC'GTr
Cuts at: 1735 1735
Size: 4382
BsaBI GATnn'nnATC
Cuts at: 6 610 1896 3933 6
Size: 604 1286 2037 455
Fragments arranged by size:
2037 1286 604 455
BsaHI Gr'CG_yC
Cuts at: 112 278 1813 2061 2763 4259 112
Size: 166 1535 248 702 1496 235
Fragments arranged by size:
1535 1496 702 248 235 166
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 409 1215 1807 2565 3142 3638 409
Size: 806 592 758 577 496 1153
Fragments arranged by size:
1153 806 758 592 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1108 1105
Size: 3 4379
BsiEI CG_ry'CG
Cuts at: 104 1924 1970 3178 104
Size: 1820 46 1208 1308
Fragments arranged by size:
1820 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 245 383 711 1396 2174 2364 3156 245
Size: 138 328 685 778 190 792 1471
Fragments arranged by size:
1471 792 778 685 328 190 138
Bsp1286I G_dGCh'C
Cuts at: 245 252 383 711 1336 1396 2007 2100 2174
Size: 7 131 328 625 60 611 93 74
Cuts at: 2174 2364 2426 3156 245
Size: 190 62 730 1471
Fragments arranged by size:
1471 730 625 611 328 190 131 93
74 62 60 7
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1432 1646 1948 2329 1432
Size: 214 302 381 3485
Fragments arranged by size:
3485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1272 2674 2728 4212 4377 50
Size: 1222 1402 54 1484 165 55
Fragments arranged by size:
1484 1402 1222 165 55 54
BsrDI GCAATG_nn'
Cuts at: 885 1003 2294 885
Size: 118 1291 2973
Fragments arranged by size:
2973 1291 118
BsrFI r'CCGG_y
Cuts at: 2380 2561 2770 2380
Size: 181 209 3992
Fragments arranged by size:
3992 209 181
BsrGI T'GTAC_A
Cuts at: 258 1317 258
Size: 1059 3323
BssSI C'ACGA_G
Cuts at: 889 2653 3011 889
Size: 1764 358 2260
Fragments arranged by size:
2260 1764 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 329 2743 329
Size: 2414 1968
BstYI r'GATC_y
Cuts at: 1 1407 1874 1897 2232 2478 3490 3576 3588
Size: 1406 467 23 335 246 1012 86 12
Cuts at: 3588 3934 1
Size: 346 449
Fragments arranged by size:
1406 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1100 1100
Size: 4382
BtgI C'CryG_G
Cuts at: 107 746 1283 107
Size: 639 537 3206
Fragments arranged by size:
3206 639 537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 1625 1625
Size: 4382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 180 215 180
Size: 35 4347
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 1177 2088 2946 1177
Size: 911 858 2613
Fragments arranged by size:
2613 911 858
EaeI y'GGCC_r
Cuts at: 1967 2141 2532 2559 3904 1967
Size: 174 391 27 1345 2445
Fragments arranged by size:
2445 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 361 2405 2615 361
Size: 2044 210 2128
Fragments arranged by size:
2128 2044 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 2900 3046 4192 2590
Size: 310 146 1146 2780
Fragments arranged by size:
2780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 709 1394 709
Size: 685 3697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 712 1270 2207 2639 3386 3654 142
Size: 570 558 937 432 747 268 870
Fragments arranged by size:
937 870 747 570 558 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1521 1521
Size: 4382
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
EcoRV GAT'ATC
Cuts at: 612 612
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1017 2401 1017
Size: 1384 2998
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1816 2064 3086 4065 1816
Size: 248 1022 979 2133
Fragments arranged by size:
2133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 662 694 2224 2254 2256 2286 662
Size: 32 1530 30 2 30 2758
Fragments arranged by size:
2758 1530 32 30 30 2
HincII GTy'rAC
Cuts at: 1421 1421
Size: 4382
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 168 749 3053 3237 168
Size: 581 2304 184 1313
Fragments arranged by size:
2304 1313 581 184
MscI TGG'CCA
Cuts at: 2143 2143
Size: 4382
MslI CAynn'nnrTG
Cuts at: 2371 2498 2371
Size: 127 4255
MspA1I CmG'CkG
Cuts at: 519 1493 1807 2167 3180 3425 519
Size: 974 314 360 1013 245 1476
Fragments arranged by size:
1476 1013 974 360 314 245
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 107 746 1283 107
Size: 639 537 3206
Fragments arranged by size:
3206 639 537
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NheI G'CTAG_C
Cuts at: 558 558
Size: 4382
NruI TCG'CGA
Cuts at: 971 971
Size: 4382
NsiI A_TGCA'T
Cuts at: 885 885
Size: 4382
NspI r_CATG'y
Cuts at: 535 1435 1935 2842 535
Size: 900 500 907 2075
Fragments arranged by size:
2075 907 900 500
PciI A'CATG_T
Cuts at: 2838 2838
Size: 4382
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 4350
PsiI TTA'TAA
Cuts at: 1016 1016
Size: 4382
PstI C_TGCA'G
Cuts at: 1429 2114 1429
Size: 685 3697
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 519 1807 2167 519
Size: 1288 360 2734
Fragments arranged by size:
2734 1288 360
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 711 1396 711
Size: 685 3697
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 361 2405 2615 361
Size: 2044 210 2128
Fragments arranged by size:
2128 2044 210
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SexAI A'CCwGG_T
Cuts at: 938 938
Size: 4382
SfcI C'TryA_G
Cuts at: 33 1425 2110 3103 3294 33
Size: 1392 685 993 191 1121
Fragments arranged by size:
1392 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 158 478 1537 2452 2944 3206 158
Size: 320 1059 915 492 262 1334
Fragments arranged by size:
1334 1059 915 492 320 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
SspI AAT'ATT
Cuts at: 1026 1026
Size: 4382
StyI C'CwwG_G
Cuts at: 107 272 746 1283 1516 1870 107
Size: 165 474 537 233 354 2619
Fragments arranged by size:
2619 537 474 354 233 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1965 2098 2557 2766 1965
Size: 133 459 209 3581
Fragments arranged by size:
3581 459 209 133
TatI w'GTAC_w
Cuts at: 258 819 1317 3897 258
Size: 561 498 2580 743
Fragments arranged by size:
2580 743 561 498
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 4350
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1877 1877
Size: 4382
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NruI NsiI NspI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I ClaI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NotI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI