Restriction map for
pF1KB3113
(Circular) (Six-base) MAPSORT of: pF1KB3113.seq Check: 9618 from: 1 to: 4082
pF1KB3113 4082 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1120 1120
Size: 4082
Acc65I G'GTAC_C
Cuts at: 1098 1098
Size: 4082
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 972 979 1907 2339 3086 3354 608
Size: 364 7 928 432 747 268 1336
Fragments arranged by size:
1336 928 747 432 364 268 7
AflII C'TTAA_G
Cuts at: 789 789
Size: 4082
AflIII A'CryG_T
Cuts at: 348 1434 2538 3515 348
Size: 1086 1104 977 915
Fragments arranged by size:
1104 1086 977 915
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AleI CACnn'nnGTG
Cuts at: 1009 1009
Size: 4082
AlwNI CAG_nnn'CTG
Cuts at: 645 1387 2954 645
Size: 742 1567 1773
Fragments arranged by size:
1773 1567 742
ApaLI G'TGCA_C
Cuts at: 552 2852 552
Size: 2300 1782
ApoI r'AATT_y
Cuts at: 741 853 1086 3435 3691 741
Size: 112 233 2349 256 1132
Fragments arranged by size:
2349 1132 256 233 112
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 1102 1102
Size: 4082
BamHI G'GATC_C
Cuts at: 261 528 1107 261
Size: 267 579 3236
Fragments arranged by size:
3236 579 267
BanI G'GyrC_C
Cuts at: 1098 1512 1760 1795 1098
Size: 414 248 35 3385
Fragments arranged by size:
3385 414 248 35
BanII G_rGCy'C
Cuts at: 1003 1096 2126 1003
Size: 93 1030 2959
Fragments arranged by size:
2959 1030 93
BbeI G_GCGC'C
Cuts at: 1516 1764 1516
Size: 248 3834
BbsI GAAGACnn'nnnn_
Cuts at: 985 985
Size: 4082
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 636 1602 636
Size: 966 3116
BglII A'GATC_T
Cuts at: 1043 1597 3634 1043
Size: 554 2037 1491
Fragments arranged by size:
2037 1491 554
BlpI GC'TnA_GC
Cuts at: 1194 1194
Size: 4082
Bme1580I G_kGCm'C
Cuts at: 556 1707 1800 2856 556
Size: 1151 93 1056 1782
Fragments arranged by size:
1782 1151 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1294 1375 1710 3854 1294
Size: 81 335 2144 1522
Fragments arranged by size:
2144 1522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1022 2449 1022
Size: 1427 2655
Bpu10I CC'TnA_GC
Cuts at: 280 440 280
Size: 160 3922
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 732 1258 2137 2629 2927 732
Size: 526 879 492 298 1887
Fragments arranged by size:
1887 879 526 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 602 3786 602
Size: 3184 898
BsaAI yAC'GTr
Cuts at: 1435 1435
Size: 4082
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 148 156 1513 1761 2463 3959 148
Size: 8 1357 248 702 1496 271
Fragments arranged by size:
1496 1357 702 271 248 8
BsaWI w'CCGG_w
Cuts at: 1271 1792 2470 2744 2891 3302 1271
Size: 521 678 274 147 411 2051
Fragments arranged by size:
2051 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 274 782 1507 2265 2842 3338 274
Size: 508 725 758 577 496 1018
Fragments arranged by size:
1018 758 725 577 508 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 677
Size: 4082
BsiEI CG_ry'CG
Cuts at: 104 1624 1670 2878 104
Size: 1520 46 1208 1308
Fragments arranged by size:
1520 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 556 1096 1874 2064 2856 556
Size: 540 778 190 792 1782
Fragments arranged by size:
1782 792 778 540 190
Bsp1286I G_dGCh'C
Cuts at: 556 1003 1096 1707 1800 1874 2064 2126 2856
Size: 447 93 611 93 74 190 62 730
Cuts at: 2856 556
Size: 1782
Fragments arranged by size:
1782 730 611 447 190 93 93 74
62
BspEI T'CCGG_A
Cuts at: 3302 3302
Size: 4082
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 197 1132 1346 1648 2029 197
Size: 935 214 302 381 2250
Fragments arranged by size:
2250 935 381 302 214
BsrBI CCG'CTC
Cuts at: 50 130 2374 2428 3912 4077 50
Size: 80 2244 54 1484 165 55
Fragments arranged by size:
2244 1484 165 80 55 54
BsrDI GCAATG_nn'
Cuts at: 1994 1994
Size: 4082
BsrFI r'CCGG_y
Cuts at: 2080 2261 2470 2080
Size: 181 209 3692
Fragments arranged by size:
3692 209 181
BssSI C'ACGA_G
Cuts at: 2353 2711 2353
Size: 358 3724
BstAPI GCAn_nnn'nTGC
Cuts at: 1459 1459
Size: 4082
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstEII G'GTnAC_C
Cuts at: 750 750
Size: 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 610 1011 610
Size: 401 3681
BstYI r'GATC_y
Cuts at: 1 261 528 1043 1107 1574 1597 1932 2178
Size: 260 267 515 64 467 23 335 246
Cuts at: 2178 3190 3276 3288 3634 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 515 467 449 346 335 267 260
246 86 64 23 12
Bsu36I CC'TnA_GG
Cuts at: 630 630
Size: 4082
BtgI C'CryG_G
Cuts at: 159 159
Size: 4082
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 216 386 2032 2213 2376 216
Size: 170 1646 181 163 1922
Fragments arranged by size:
1922 1646 181 170 163
BtsI GCAGTG_nn'
Cuts at: 395 415 1325 395
Size: 20 910 3152
Fragments arranged by size:
3152 910 20
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 1667 1841 2232 2259 3604 1667
Size: 174 391 27 1345 2145
Fragments arranged by size:
2145 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1667 1667
Size: 4082
EarI CTCTTCn'nnn_
Cuts at: 944 2105 2315 944
Size: 1161 210 2711
Fragments arranged by size:
2711 1161 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 972 979 1907 2339 3086 3354 608
Size: 364 7 928 432 747 268 1336
Fragments arranged by size:
1336 928 747 432 364 268 7
EcoO109I rG'GnC_Cy
Cuts at: 1221 1221
Size: 4082
EcoRI G'AATT_C
Cuts at: 1086 1086
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 1516 1764 2786 3765 1516
Size: 248 1022 979 1833
Fragments arranged by size:
1833 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 665 697 1924 1954 1956 1986 665
Size: 32 1227 30 2 30 2761
Fragments arranged by size:
2761 1227 32 30 30 2
HincII GTy'rAC
Cuts at: 765 1121 765
Size: 356 3726
HindIII A'AGCT_T
Cuts at: 294 294
Size: 4082
KasI G'GCGC_C
Cuts at: 1512 1760 1512
Size: 248 3834
KpnI G_GTAC'C
Cuts at: 1102 1102
Size: 4082
MluI A'CGCG_T
Cuts at: 3515 3515
Size: 4082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 519 556 2753 2937 519
Size: 37 2197 184 1664
Fragments arranged by size:
2197 1664 184 37
MscI TGG'CCA
Cuts at: 1843 1843
Size: 4082
MslI CAynn'nnrTG
Cuts at: 833 1009 2071 2198 833
Size: 176 1062 127 2717
Fragments arranged by size:
2717 1062 176 127
MspA1I CmG'CkG
Cuts at: 1193 1507 1867 2880 3125 1193
Size: 314 360 1013 245 2150
Fragments arranged by size:
2150 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2263 2263
Size: 4082
NarI GG'CG_CC
Cuts at: 1513 1761 1513
Size: 248 3834
NgoMIV G'CCGG_C
Cuts at: 2261 2261
Size: 4082
NspI r_CATG'y
Cuts at: 352 1135 1635 2542 352
Size: 783 500 907 1892
Fragments arranged by size:
1892 907 783 500
PciI A'CATG_T
Cuts at: 348 2538 348
Size: 2190 1892
PflMI CCAn_nnn'nTGG
Cuts at: 538 538
Size: 4082
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 338 370 3221 3253 338
Size: 32 2851 32 1167
Fragments arranged by size:
2851 1167 32 32
PshAI GACnn'nnGTC
Cuts at: 989 989
Size: 4082
PstI C_TGCA'G
Cuts at: 386 411 1129 1814 386
Size: 25 718 685 2654
Fragments arranged by size:
2654 718 685 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 1507 1867 1507
Size: 360 3722
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 3599 3599
Size: 4082
SfcI C'TryA_G
Cuts at: 33 363 382 407 768 1125 1810 2803 2994
Size: 330 19 25 361 357 685 993 191
Cuts at: 2994 33
Size: 1121
Fragments arranged by size:
1121 993 685 361 357 330 191 25
19
SfoI GGC'GCC
Cuts at: 1514 1762 1514
Size: 248 3834
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 1104 1104
Size: 4082
SmlI C'TyrA_G
Cuts at: 711 789 1237 2152 2644 2906 711
Size: 78 448 915 492 262 1887
Fragments arranged by size:
1887 915 492 448 262 78
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
SspI AAT'ATT
Cuts at: 257 257
Size: 4082
StyI C'CwwG_G
Cuts at: 1216 1570 1216
Size: 354 3728
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1665 1798 2257 2466 1665
Size: 133 459 209 3281
Fragments arranged by size:
3281 459 209 133
TatI w'GTAC_w
Cuts at: 3597 3597
Size: 4082
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4050
Tth111I GACn'n_nGTC
Cuts at: 1879 1879
Size: 4082
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1577 1577
Size: 4082
XmaI C'CCGG_G
Cuts at: 1102 1102
Size: 4082
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI