Restriction map for
pF1KB3110
(Circular) (Six-base) MAPSORT of: pF1KB3110.seq Check: 6945 from: 1 to: 4004
pF1KB3110 4004 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 215 727 1020 215
Size: 512 293 3199
Fragments arranged by size:
3199 512 293
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1829 2261 3008 3276 1829
Size: 432 747 268 2557
Fragments arranged by size:
2557 747 432 268
AflIII A'CryG_T
Cuts at: 847 1356 2460 3437 847
Size: 509 1104 977 1414
Fragments arranged by size:
1414 1104 977 509
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 613 645 613
Size: 32 3972
AlwNI CAG_nnn'CTG
Cuts at: 192 201 1309 2876 192
Size: 9 1108 1567 1320
Fragments arranged by size:
1567 1320 1108 9
ApaI G_GGCC'C
Cuts at: 174 174
Size: 4004
ApaLI G'TGCA_C
Cuts at: 2774 2774
Size: 4004
ApoI r'AATT_y
Cuts at: 1008 3357 3613 1008
Size: 2349 256 1399
Fragments arranged by size:
2349 1399 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 270 337 656 688 695 961 1024 270
Size: 67 319 32 7 266 63 3250
Fragments arranged by size:
3250 319 266 67 63 32 7
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 215 274 679 727 1020 1434 1682 1717 215
Size: 59 405 48 293 414 248 35 2502
Fragments arranged by size:
2502 414 405 293 248 59 48 35
BanII G_rGCy'C
Cuts at: 174 689 695 784 1018 2048 174
Size: 515 6 89 234 1030 2130
Fragments arranged by size:
2130 1030 515 234 89 6
BbeI G_GCGC'C
Cuts at: 278 1438 1686 278
Size: 1160 248 2596
Fragments arranged by size:
2596 1160 248
BbsI GAAGACnn'nnnn_
Cuts at: 997 997
Size: 4004
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 1524 1524
Size: 4004
BglI GCCn_nnn'nGGC
Cuts at: 374 374
Size: 4004
BglII A'GATC_T
Cuts at: 1519 3556 1519
Size: 2037 1967
BlpI GC'TnA_GC
Cuts at: 532 575 1116 532
Size: 43 541 3420
Fragments arranged by size:
3420 541 43
Bme1580I G_kGCm'C
Cuts at: 174 917 1629 1722 2778 174
Size: 743 712 93 1056 1400
Fragments arranged by size:
1400 1056 743 712 93
BmgBI CAC'GTC
Cuts at: 396 912 396
Size: 516 3488
BmrI ACTGGGnnnn_n'
Cuts at: 1216 1297 1632 3776 1216
Size: 81 335 2144 1444
Fragments arranged by size:
2144 1444 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 793
Size: 4004
Bpu10I CC'TnA_GC
Cuts at: 757 757
Size: 4004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 2059 2551 2849 1180
Size: 879 492 298 2335
Fragments arranged by size:
2335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3708 3708
Size: 4004
BsaAI yAC'GTr
Cuts at: 848 876 1357 848
Size: 28 481 3495
Fragments arranged by size:
3495 481 28
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 206 275 410 860 1435 1683 2385 3881 206
Size: 69 135 450 575 248 702 1496 329
Fragments arranged by size:
1496 702 575 450 329 248 135 69
BsaWI w'CCGG_w
Cuts at: 1193 1714 2392 2666 2813 3224 1193
Size: 521 678 274 147 411 1973
Fragments arranged by size:
1973 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 913 943 913
Size: 30 3974
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 802 271
Size: 531 3473
BseYI C'CCAG_C
Cuts at: 181 228 1429 2187 2764 3260 181
Size: 47 1201 758 577 496 925
Fragments arranged by size:
1201 925 758 577 496 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 321
Size: 4004
BsiEI CG_ry'CG
Cuts at: 104 309 1546 1592 2800 104
Size: 205 1237 46 1208 1308
Fragments arranged by size:
1308 1237 1208 205 46
BsiHKAI G_wGCw'C
Cuts at: 1018 1796 1986 2778 1018
Size: 778 190 792 2244
Fragments arranged by size:
2244 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 692 692
Size: 4004
Bsp1286I G_dGCh'C
Cuts at: 174 689 695 784 917 1018 1629 1722 1796
Size: 515 6 89 133 101 611 93 74
Cuts at: 1796 1986 2048 2778 174
Size: 190 62 730 1400
Fragments arranged by size:
1400 730 611 515 190 133 101 93
89 74 62 6
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 4004
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 227 561 659 1054 1268 1570 1951 227
Size: 334 98 395 214 302 381 2280
Fragments arranged by size:
2280 395 381 334 302 214 98
BsrBI CCG'CTC
Cuts at: 50 531 2296 2350 3834 3999 50
Size: 481 1765 54 1484 165 55
Fragments arranged by size:
1765 1484 481 165 55 54
BsrDI GCAATG_nn'
Cuts at: 154 1916 154
Size: 1762 2242
BsrFI r'CCGG_y
Cuts at: 2002 2183 2392 2002
Size: 181 209 3614
Fragments arranged by size:
3614 209 181
BssHII G'CGCG_C
Cuts at: 131 177 951 131
Size: 46 774 3184
Fragments arranged by size:
3184 774 46
BssSI C'ACGA_G
Cuts at: 2275 2633 2275
Size: 358 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 192 1381 192
Size: 1189 2815
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstYI r'GATC_y
Cuts at: 1 791 1029 1496 1519 1854 2100 3112 3198
Size: 790 238 467 23 335 246 1012 86
Cuts at: 3198 3210 3556 1
Size: 12 346 449
Fragments arranged by size:
1012 790 467 449 346 335 246 238
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 785 785
Size: 4004
BtgI C'CryG_G
Cuts at: 107 675 107
Size: 568 3436
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 484 1247 484
Size: 763 3241
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DraIII CAC_nnn'GTG
Cuts at: 399 480 399
Size: 81 3923
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 386 468 714 842 1589 1763 2154 2181 3526
Size: 82 246 128 747 174 391 27 1345
Cuts at: 3526 386
Size: 864
Fragments arranged by size:
1345 864 747 391 246 174 128 82
27
EagI C'GGCC_G
Cuts at: 1589 1589
Size: 4004
EarI CTCTTCn'nnn_
Cuts at: 772 2027 2237 772
Size: 1255 210 2539
Fragments arranged by size:
2539 1255 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 172 423 493 784 2212 2522 2668 3814 172
Size: 251 70 291 1428 310 146 1146 362
Fragments arranged by size:
1428 1146 362 310 291 251 146 70
EcoICRI GAG'CTC
Cuts at: 1016 1016
Size: 4004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1829 2261 3008 3276 793
Size: 1036 432 747 268 1521
Fragments arranged by size:
1521 1036 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 760 760
Size: 4004
EcoO109I rG'GnC_Cy
Cuts at: 171 423 887 1143 171
Size: 252 464 256 3032
Fragments arranged by size:
3032 464 256 252
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 4004
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 166 264 278 639 1438 1686 2708 3687 166
Size: 98 14 361 799 248 1022 979 483
Fragments arranged by size:
1022 979 799 483 361 248 98 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 783 815 958 990 1846 1876 1878
Size: 32 453 32 143 32 856 30 2
Cuts at: 1878 1908 298
Size: 30 2394
Fragments arranged by size:
2394 856 453 143 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1043 1043
Size: 4004
KasI G'GCGC_C
Cuts at: 274 1434 1682 274
Size: 1160 248 2596
Fragments arranged by size:
2596 1160 248
KpnI G_GTAC'C
Cuts at: 219 731 1024 219
Size: 512 293 3199
Fragments arranged by size:
3199 512 293
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2859 2675
Size: 184 3820
MscI TGG'CCA
Cuts at: 716 844 1765 716
Size: 128 921 2955
Fragments arranged by size:
2955 921 128
MslI CAynn'nnrTG
Cuts at: 1993 2120 1993
Size: 127 3877
MspA1I CmG'CkG
Cuts at: 198 677 1115 1429 1789 2802 3047 198
Size: 479 438 314 360 1013 245 1155
Fragments arranged by size:
1155 1013 479 438 360 314 245
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 275 1435 1683 275
Size: 1160 248 2596
Fragments arranged by size:
2596 1160 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4004
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 333 951 1057 1557 2464 333
Size: 618 106 500 907 1873
Fragments arranged by size:
1873 907 618 500 106
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PflMI CCAn_nnn'nTGG
Cuts at: 648 648
Size: 4004
PfoI T'CCnGG_A
Cuts at: 806 806
Size: 4004
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PmlI CAC'GTG
Cuts at: 848 876 848
Size: 28 3976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3972
PspOMI G'GGCC_C
Cuts at: 170 170
Size: 4004
PstI C_TGCA'G
Cuts at: 197 1051 1736 197
Size: 854 685 2465
Fragments arranged by size:
2465 854 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 198 1429 1789 198
Size: 1231 360 2413
Fragments arranged by size:
2413 1231 360
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 1018 1018
Size: 4004
SacII CC_GC'GG
Cuts at: 678 678
Size: 4004
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 772 2027 2237 772
Size: 1255 210 2539
Fragments arranged by size:
2539 1255 210
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SfcI C'TryA_G
Cuts at: 33 193 1047 1732 2725 2916 33
Size: 160 854 685 993 191 1121
Fragments arranged by size:
1121 993 854 685 191 160
SfoI GGC'GCC
Cuts at: 276 1436 1684 276
Size: 1160 248 2596
Fragments arranged by size:
2596 1160 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 272 339 1026 272
Size: 67 687 3250
Fragments arranged by size:
3250 687 67
SmlI C'TyrA_G
Cuts at: 1159 2074 2566 2828 1159
Size: 915 492 262 2335
Fragments arranged by size:
2335 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
StyI C'CwwG_G
Cuts at: 107 1138 1492 107
Size: 1031 354 2619
Fragments arranged by size:
2619 1031 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 141 406 924 1587 1720 2179 2388 141
Size: 265 518 663 133 459 209 1757
Fragments arranged by size:
1757 663 518 459 265 209 133
TatI w'GTAC_w
Cuts at: 3519 3519
Size: 4004
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 3972
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1499 1499
Size: 4004
XmaI C'CCGG_G
Cuts at: 270 337 1024 270
Size: 67 687 3250
Fragments arranged by size:
3250 687 67
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI
BsrGI BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI