Restriction map for
pF1KB3106
(Circular) (Six-base) MAPSORT of: pF1KB3106.seq Check: 7004 from: 1 to: 5624
pF1KB3106 5624 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 941 2662 941
Size: 1721 3903
Acc65I G'GTAC_C
Cuts at: 2153 2640 2153
Size: 487 5137
AclI AA'CG_TT
Cuts at: 977 2261 5464 977
Size: 1284 3203 1137
Fragments arranged by size:
3203 1284 1137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 770 2080 3449 3881 4234 4896 562
Size: 208 1310 1369 432 353 662 1290
Fragments arranged by size:
1369 1310 1290 662 432 353 208
AflIII A'CryG_T
Cuts at: 2976 4776 5057 2976
Size: 1800 281 3543
Fragments arranged by size:
3543 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2021 2021
Size: 5624
AlwNI CAG_nnn'CTG
Cuts at: 1741 2103 2601 2929 4367 1741
Size: 362 498 328 1438 2998
Fragments arranged by size:
2998 1438 498 362 328
ApaI G_GGCC'C
Cuts at: 321 321
Size: 5624
ApaLI G'TGCA_C
Cuts at: 1642 4462 1642
Size: 2820 2804
ApoI r'AATT_y
Cuts at: 2215 2628 4977 5233 2215
Size: 413 2349 256 2606
Fragments arranged by size:
2606 2349 413 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5624
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
AvaI C'yCGr_G
Cuts at: 610 1060 2644 610
Size: 450 1584 3590
Fragments arranged by size:
3590 1584 450
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2145 2178 2145
Size: 33 5591
BamHI G'GATC_C
Cuts at: 688 1425 1760 2649 688
Size: 737 335 889 3663
Fragments arranged by size:
3663 889 737 335
BanI G'GyrC_C
Cuts at: 1450 1850 2153 2266 2580 2640 3054 3302 3337
Size: 400 303 113 314 60 414 248 35
Cuts at: 3337 1450
Size: 3737
Fragments arranged by size:
3737 414 400 314 303 248 113 60
35
BanII G_rGCy'C
Cuts at: 321 362 2638 3668 321
Size: 41 2276 1030 2277
Fragments arranged by size:
2277 2276 1030 41
BbeI G_GCGC'C
Cuts at: 3058 3306 3058
Size: 248 5376
BbsI GAAGACnn'nnnn_
Cuts at: 1893 2460 2578 1893
Size: 567 118 4939
Fragments arranged by size:
4939 567 118
BbvCI CC'TCA_GC
Cuts at: 1225 2450 1225
Size: 1225 4399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1849 1883 1849
Size: 34 5590
BciVI GTATCCnnnnn_n'
Cuts at: 3522 4578 5624 3522
Size: 1056 1046 3522
Fragments arranged by size:
3522 1056 1046
BclI T'GATC_A
Cuts at: 3144 3144
Size: 5624
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5624
BglII A'GATC_T
Cuts at: 3139 5176 3139
Size: 2037 3587
BlpI GC'TnA_GC
Cuts at: 772 2736 772
Size: 1964 3660
Bme1580I G_kGCm'C
Cuts at: 321 1311 1646 1949 2417 2585 3249 3342 4466
Size: 990 335 303 468 168 664 93 1124
Cuts at: 4466 321
Size: 1479
Fragments arranged by size:
1479 1124 990 664 468 335 303 168
93
BmgBI CAC'GTC
Cuts at: 2576 2576
Size: 5624
BmrI ACTGGGnnnn_n'
Cuts at: 201 1584 2037 2836 2917 3252 5396 201
Size: 1383 453 799 81 335 2144 429
Fragments arranged by size:
2144 1383 799 453 429 335 81
BmtI G_CTAG'C
Cuts at: 2408 2408
Size: 5624
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 297 1479 3991 297
Size: 1182 2512 1930
Fragments arranged by size:
2512 1930 1182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1087 1195 1258 1816 1867 184
Size: 903 108 63 558 51 3941
Fragments arranged by size:
3941 903 558 108 63 51
Bpu10I CC'TnA_GC
Cuts at: 1225 2450 1225
Size: 1225 4399
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 884 2800 3679 4393 4691 346
Size: 538 1916 879 714 298 1279
Fragments arranged by size:
1916 1279 879 714 538 298
BsaI GGTCTCn'nnnn_
Cuts at: 2434 5328 2434
Size: 2894 2730
BsaAI yAC'GTr
Cuts at: 2977 2977
Size: 5624
BsaBI GATnn'nnATC
Cuts at: 6 2536 3138 5175 6
Size: 2530 602 2037 455
Fragments arranged by size:
2530 2037 602 455
BsaHI Gr'CG_yC
Cuts at: 3055 3303 4005 5501 3055
Size: 248 702 1496 3178
Fragments arranged by size:
3178 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2813 3334 4012 4423 4570 4844 2813
Size: 521 678 411 147 274 3593
Fragments arranged by size:
3593 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 373
Size: 30 5594
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 394 769 1219 1264 1360 1854 2152 2467 394
Size: 375 450 45 96 494 298 315 3551
Fragments arranged by size:
3551 494 450 375 315 298 96 45
BseYI C'CCAG_C
Cuts at: 298 487 539 1855 1953 3049 3807 4472 4880
Size: 189 52 1316 98 1096 758 665 408
Cuts at: 4880 298
Size: 1042
Fragments arranged by size:
1316 1096 1042 758 665 408 189 98
52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1425 1425
Size: 5624
BsiEI CG_ry'CG
Cuts at: 104 1365 2544 3166 3212 4442 104
Size: 1261 1179 622 46 1230 1286
Fragments arranged by size:
1286 1261 1230 1179 622 46
BsiHKAI G_wGCw'C
Cuts at: 1073 1646 2110 2289 2459 2638 3416 3606 4466
Size: 573 464 179 170 179 778 190 860
Cuts at: 4466 1073
Size: 2231
Fragments arranged by size:
2231 860 778 573 464 190 179 179
170
BsmI GAATG_Cn'
Cuts at: 624 1924 2008 624
Size: 1300 84 4240
Fragments arranged by size:
4240 1300 84
Bsp1286I G_dGCh'C
Cuts at: 321 362 1073 1311 1646 1949 2110 2289 2417
Size: 41 711 238 335 303 161 179 128
Cuts at: 2417 2459 2585 2638 3249 3342 3416 3606 3668
Size: 42 126 53 611 93 74 190 62
Cuts at: 3668 4466 321
Size: 798 1479
Fragments arranged by size:
1479 798 711 611 335 303 238 190
179 161 128 126 93 74 62 53
42 41
BspHI T'CATG_A
Cuts at: 1400 2237 4056 1400
Size: 837 1819 2968
Fragments arranged by size:
2968 1819 837
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1085 2674 2888 3190 3571 1085
Size: 1589 214 302 381 3138
Fragments arranged by size:
3138 1589 381 302 214
BsrBI CCG'CTC
Cuts at: 50 168 1143 1209 1260 1731 2322 3916 3970
Size: 118 975 66 51 471 591 1594 54
Cuts at: 3970 5454 5619 50
Size: 1484 165 55
Fragments arranged by size:
1594 1484 975 591 471 165 118 66
55 54 51
BsrDI GCAATG_nn'
Cuts at: 2297 3536 2297
Size: 1239 4385
BsrFI r'CCGG_y
Cuts at: 1124 3622 3803 1124
Size: 2498 181 2945
Fragments arranged by size:
2945 2498 181
BssSI C'ACGA_G
Cuts at: 1201 3895 4603 1201
Size: 2694 708 2222
Fragments arranged by size:
2694 2222 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1077 3001 1077
Size: 1924 3700
BstBI TT'CG_AA
Cuts at: 1319 1575 3985 1319
Size: 256 2410 2958
Fragments arranged by size:
2958 2410 256
BstYI r'GATC_y
Cuts at: 1 195 688 1425 1661 1760 2649 3116 3139
Size: 194 493 737 236 99 889 467 23
Cuts at: 3139 3474 3720 4026 4038 4124 5176 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 889 737 493 467 449 335 306
246 236 194 99 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 440 440
Size: 5624
BtgI C'CryG_G
Cuts at: 833 1506 1768 833
Size: 673 262 4689
Fragments arranged by size:
4689 673 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3574 3755 3918 3574
Size: 181 163 5280
Fragments arranged by size:
5280 181 163
BtsI GCAGTG_nn'
Cuts at: 875 2590 2867 875
Size: 1715 277 3632
Fragments arranged by size:
3632 1715 277
DraI TTT'AAA
Cuts at: 2623 2623
Size: 5624
DraIII CAC_nnn'GTG
Cuts at: 1592 2150 1592
Size: 558 5066
DrdI GACnn_nn'nnGTC
Cuts at: 1153 3330 4674 1153
Size: 2177 1344 2103
Fragments arranged by size:
2177 2103 1344
EaeI y'GGCC_r
Cuts at: 1382 2399 2520 2612 3209 3383 3774 3801 5146
Size: 1017 121 92 597 174 391 27 1345
Cuts at: 5146 1382
Size: 1860
Fragments arranged by size:
1860 1345 1017 597 391 174 121 92
27
EagI C'GGCC_G
Cuts at: 3209 3209
Size: 5624
EarI CTCTTCn'nnn_
Cuts at: 404 746 833 1168 3647 3857 404
Size: 342 87 335 2479 210 2171
Fragments arranged by size:
2479 2171 342 335 210 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1278 1526 3832 4574 4720 5434 1278
Size: 248 2306 742 146 714 1468
Fragments arranged by size:
2306 1468 742 714 248 146
EcoICRI GAG'CTC
Cuts at: 2636 2636
Size: 5624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 562 770 1087 1195 1258 1816 1867 2080
Size: 378 208 317 108 63 558 51 213
Cuts at: 2080 3449 3881 4234 4896 184
Size: 1369 432 353 662 912
Fragments arranged by size:
1369 912 662 558 432 378 353 317
213 208 108 63 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1228 1539 1783 1228
Size: 311 244 5069
Fragments arranged by size:
5069 311 244
EcoO109I rG'GnC_Cy
Cuts at: 606 1041 1191 1233 1518 2430 2763 606
Size: 435 150 42 285 912 333 3467
Fragments arranged by size:
3467 912 435 333 285 150 42
EcoRI G'AATT_C
Cuts at: 2628 2628
Size: 5624
EcoRV GAT'ATC
Cuts at: 2235 2235
Size: 5624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1428 1917 2097 3643 1428
Size: 489 180 1546 3409
Fragments arranged by size:
3409 1546 489 180
FspI TGC'GCA
Cuts at: 3405 3405
Size: 5624
HaeII r_GCGC'y
Cuts at: 231 1838 3058 3306 4536 5307 231
Size: 1607 1220 248 1230 771 548
Fragments arranged by size:
1607 1230 1220 771 548 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 593 625 1505 1537 3466 3496 3498 3528 593
Size: 32 880 32 1929 30 2 30 2689
Fragments arranged by size:
2689 1929 880 32 32 30 30 2
HincII GTy'rAC
Cuts at: 342 732 1158 2663 342
Size: 390 426 1505 3303
Fragments arranged by size:
3303 1505 426 390
HindIII A'AGCT_T
Cuts at: 571 571
Size: 5624
KasI G'GCGC_C
Cuts at: 3054 3302 3054
Size: 248 5376
KpnI G_GTAC'C
Cuts at: 2157 2644 2157
Size: 487 5137
MfeI C'AATT_G
Cuts at: 641 641
Size: 5624
MluI A'CGCG_T
Cuts at: 5057 5057
Size: 5624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 371 1385 2143 2282 4383 4567 371
Size: 1014 758 139 2101 184 1428
Fragments arranged by size:
2101 1428 1014 758 184 139
MscI TGG'CCA
Cuts at: 1384 2614 3385 1384
Size: 1230 771 3623
Fragments arranged by size:
3623 1230 771
MslI CAynn'nnrTG
Cuts at: 916 2003 2395 3613 3740 916
Size: 1087 392 1218 127 2800
Fragments arranged by size:
2800 1218 1087 392 127
MspA1I CmG'CkG
Cuts at: 376 539 1014 1083 2735 3049 3409 4193 4438
Size: 163 475 69 1652 314 360 784 245
Cuts at: 4438 376
Size: 1562
Fragments arranged by size:
1652 1562 784 475 360 314 245 163
69
NaeI GCC'GGC
Cuts at: 3805 3805
Size: 5624
NarI GG'CG_CC
Cuts at: 3055 3303 3055
Size: 248 5376
NcoI C'CATG_G
Cuts at: 1506 1768 1506
Size: 262 5362
NgoMIV G'CCGG_C
Cuts at: 3803 3803
Size: 5624
NheI G'CTAG_C
Cuts at: 2404 2404
Size: 5624
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5624
NspI r_CATG'y
Cuts at: 2277 2677 3177 4780 2277
Size: 400 500 1603 3121
Fragments arranged by size:
3121 1603 500 400
PciI A'CATG_T
Cuts at: 4776 4776
Size: 5624
PflMI CCAn_nnn'nTGG
Cuts at: 1024 1173 2190 1024
Size: 149 1017 4458
Fragments arranged by size:
4458 1017 149
PfoI T'CCnGG_A
Cuts at: 1193 1235 1193
Size: 42 5582
PmeI GTTT'AAAC
Cuts at: 2623 2623
Size: 5624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 4070
Size: 32 5592
PpuMI rG'GwC_Cy
Cuts at: 1191 1233 1518 2430 1191
Size: 42 285 912 4385
Fragments arranged by size:
4385 912 285 42
PshAI GACnn'nnGTC
Cuts at: 1189 1189
Size: 5624
PspOMI G'GGCC_C
Cuts at: 317 317
Size: 5624
PstI C_TGCA'G
Cuts at: 1082 1282 1331 1844 2597 2671 3356 1082
Size: 200 49 513 753 74 685 3350
Fragments arranged by size:
3350 753 685 513 200 74 49
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5624
PvuII CAG'CTG
Cuts at: 539 1014 1083 3049 3409 539
Size: 475 69 1966 360 2754
Fragments arranged by size:
2754 1966 475 360 69
RsrII CG'GwC_CG
Cuts at: 3819 3819
Size: 5624
SacI G_AGCT'C
Cuts at: 2638 2638
Size: 5624
SalI G'TCGA_C
Cuts at: 2661 2661
Size: 5624
SapI GCTCTTCn'nnn_
Cuts at: 3647 3857 3647
Size: 210 5414
SbfI CC_TGCA'GG
Cuts at: 1331 2671 1331
Size: 1340 4284
ScaI AGT'ACT
Cuts at: 1339 1411 5141 1339
Size: 72 3730 1822
Fragments arranged by size:
3730 1822 72
SfcI C'TryA_G
Cuts at: 33 945 951 1000 1078 1132 1278 1327 1840
Size: 912 6 49 78 54 146 49 513
Cuts at: 1840 2593 2667 3352 4320 4511 33
Size: 753 74 685 968 191 1146
Fragments arranged by size:
1146 968 912 753 685 513 191 146
78 74 54 49 49 6
SfoI GGC'GCC
Cuts at: 3056 3304 3056
Size: 248 5376
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
SmaI CCC'GGG
Cuts at: 2646 2646
Size: 5624
SmlI C'TyrA_G
Cuts at: 361 899 2779 3694 4408 4670 361
Size: 538 1880 915 714 262 1315
Fragments arranged by size:
1880 1315 915 714 538 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5624
SphI G_CATG'C
Cuts at: 2677 3177 2677
Size: 500 5124
SspI AAT'ATT
Cuts at: 1581 1581
Size: 5624
StyI C'CwwG_G
Cuts at: 242 454 851 1037 1506 1600 1768 2444 2615
Size: 212 397 186 469 94 168 676 171
Cuts at: 2615 2758 3112 242
Size: 143 354 2754
Fragments arranged by size:
2754 676 469 397 354 212 186 171
168 143 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1201 1603 1629 1781 2007 2326 2568 3207 3340
Size: 402 26 152 226 319 242 639 133
Cuts at: 3340 3799 4008 1201
Size: 459 209 2817
Fragments arranged by size:
2817 639 459 402 319 242 226 209
152 133 26
TatI w'GTAC_w
Cuts at: 1337 1349 1409 5139 1337
Size: 12 60 3730 1822
Fragments arranged by size:
3730 1822 60 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5141 5173 5141
Size: 32 5592
Tth111I GACn'n_nGTC
Cuts at: 3421 3421
Size: 5624
XbaI T'CTAG_A
Cuts at: 2655 2655
Size: 5624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 522 2154 3119 522
Size: 1632 965 3027
Fragments arranged by size:
3027 1632 965
XmaI C'CCGG_G
Cuts at: 2644 2644
Size: 5624
XmnI GAAnn'nnTTC
Cuts at: 208 2332 208
Size: 2124 3500
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BglI BsiWI BsmBI BspEI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI FseI FspAI HpaI
NdeI NotI NruI PacI PasI PmlI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI