Restriction map for
pF1KB3104
(Circular) (Six-base) MAPSORT of: pF1KB3104.seq Check: 9359 from: 1 to: 5522
pF1KB3104 5522 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2131 2131
Size: 5522
AccI GT'mk_AC
Cuts at: 565 2560 565
Size: 1995 3527
Acc65I G'GTAC_C
Cuts at: 2538 2538
Size: 5522
AclI AA'CG_TT
Cuts at: 5362 5362
Size: 5522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 631 1521 1533 1774 2029 2445 3347 3779
Size: 316 890 12 241 255 416 902 432
Cuts at: 3779 4132 4794 315
Size: 353 662 1043
Fragments arranged by size:
1043 902 890 662 432 416 353 316
255 241 12
AfeI AGC'GCT
Cuts at: 132 1542 132
Size: 1410 4112
AflIII A'CryG_T
Cuts at: 720 990 1133 1662 2874 4674 4955 720
Size: 270 143 529 1212 1800 281 1287
Fragments arranged by size:
1800 1287 1212 529 281 270 143
AgeI A'CCGG_T
Cuts at: 2429 2429
Size: 5522
AleI CACnn'nnGTG
Cuts at: 834 1075 834
Size: 241 5281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 2255
Size: 32 5490
AlwNI CAG_nnn'CTG
Cuts at: 168 791 1752 2274 2424 2827 4265 168
Size: 623 961 522 150 403 1438 1425
Fragments arranged by size:
1438 1425 961 623 522 403 150
ApaI G_GGCC'C
Cuts at: 667 667
Size: 5522
ApaLI G'TGCA_C
Cuts at: 716 1612 4360 716
Size: 896 2748 1878
Fragments arranged by size:
2748 1878 896
ApoI r'AATT_y
Cuts at: 347 385 590 1297 2303 2526 4875 5131 347
Size: 38 205 707 1006 223 2349 256 738
Fragments arranged by size:
2349 1006 738 707 256 223 205 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 5522
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
AvaI C'yCGr_G
Cuts at: 358 1815 2542 358
Size: 1457 727 3338
Fragments arranged by size:
3338 1457 727
BamHI G'GATC_C
Cuts at: 603 2465 2547 603
Size: 1862 82 3578
Fragments arranged by size:
3578 1862 82
BanI G'GyrC_C
Cuts at: 1810 2538 2952 3200 3235 1810
Size: 728 414 248 35 4097
Fragments arranged by size:
4097 728 414 248 35
BanII G_rGCy'C
Cuts at: 667 905 2536 3566 667
Size: 238 1631 1030 2623
Fragments arranged by size:
2623 1631 1030 238
BbeI G_GCGC'C
Cuts at: 2956 3204 2956
Size: 248 5274
BbsI GAAGACnn'nnnn_
Cuts at: 344 823 344
Size: 479 5043
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 840 874 1083 1117 840
Size: 34 209 34 5245
Fragments arranged by size:
5245 209 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3420 4476 5522 3420
Size: 1056 1046 3420
Fragments arranged by size:
3420 1056 1046
BclI T'GATC_A
Cuts at: 2276 3042 2276
Size: 766 4756
BglI GCCn_nnn'nGGC
Cuts at: 671 671
Size: 5522
BglII A'GATC_T
Cuts at: 324 1418 3037 5074 324
Size: 1094 1619 2037 772
Fragments arranged by size:
2037 1619 1094 772
BlpI GC'TnA_GC
Cuts at: 2634 2634
Size: 5522
Bme1580I G_kGCm'C
Cuts at: 667 720 1616 3147 3240 4364 667
Size: 53 896 1531 93 1124 1825
Fragments arranged by size:
1825 1531 1124 896 93 53
BmgBI CAC'GTC
Cuts at: 1380 1380
Size: 5522
BmrI ACTGGGnnnn_n'
Cuts at: 1383 2734 2815 3150 5294 1383
Size: 1351 81 335 2144 1611
Fragments arranged by size:
2144 1611 1351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 200 3889 200
Size: 3689 1833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1045 1990 2391 491
Size: 554 945 401 3622
Fragments arranged by size:
3622 945 554 401
Bpu10I CC'TnA_GC
Cuts at: 583 1429 2071 583
Size: 846 642 4034
Fragments arranged by size:
4034 846 642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 2698 3577 4291 4589 1561
Size: 1137 879 714 298 2494
Fragments arranged by size:
2494 1137 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 753 845 5226 753
Size: 92 4381 1049
Fragments arranged by size:
4381 1049 92
BsaAI yAC'GTr
Cuts at: 2875 2875
Size: 5522
BsaBI GATnn'nnATC
Cuts at: 6 3036 5073 6
Size: 3030 2037 455
Fragments arranged by size:
3030 2037 455
BsaHI Gr'CG_yC
Cuts at: 1157 2953 3201 3903 5399 1157
Size: 1796 248 702 1496 1280
Fragments arranged by size:
1796 1496 1280 702 248
BsaWI w'CCGG_w
Cuts at: 843 2429 2711 3232 3910 4321 4468 4742 843
Size: 1586 282 521 678 411 147 274 1623
Fragments arranged by size:
1623 1586 678 521 411 282 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 904 934 904
Size: 30 5492
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 820 1987 2521 2524 820
Size: 1167 534 3 3818
Fragments arranged by size:
3818 1167 534 3
BseYI C'CCAG_C
Cuts at: 976 1325 1853 1959 2088 2947 3705 4370 4778
Size: 349 528 106 129 859 758 665 408
Cuts at: 4778 976
Size: 1720
Fragments arranged by size:
1720 859 758 665 528 408 349 129
106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 1158 1512 1924 169
Size: 989 354 412 3767
Fragments arranged by size:
3767 989 412 354
BsiEI CG_ry'CG
Cuts at: 104 1908 3064 3110 4340 104
Size: 1804 1156 46 1230 1286
Fragments arranged by size:
1804 1286 1230 1156 46
BsiHKAI G_wGCw'C
Cuts at: 720 905 1435 1616 2077 2536 3314 3504 4364
Size: 185 530 181 461 459 778 190 860
Cuts at: 4364 720
Size: 1878
Fragments arranged by size:
1878 860 778 530 461 459 190 185
181
BsmI GAATG_Cn'
Cuts at: 2461 2461
Size: 5522
BsmBI CGTCTCn'nnnn_
Cuts at: 1446 1852 1446
Size: 406 5116
Bsp1286I G_dGCh'C
Cuts at: 667 720 905 1435 1616 2077 2536 3147 3240
Size: 53 185 530 181 461 459 611 93
Cuts at: 3240 3314 3504 3566 4364 667
Size: 74 190 62 798 1825
Fragments arranged by size:
1825 798 611 530 461 459 190 185
181 93 74 62 53
BspEI T'CCGG_A
Cuts at: 843 843
Size: 5522
BspHI T'CATG_A
Cuts at: 351 3954 351
Size: 3603 1919
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1451 1745 2131 2572 2786 3088 3469 1451
Size: 294 386 441 214 302 381 3504
Fragments arranged by size:
3504 441 386 381 302 294 214
BsrBI CCG'CTC
Cuts at: 50 3814 3868 5352 5517 50
Size: 3764 54 1484 165 55
Fragments arranged by size:
3764 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 642 3434 642
Size: 2792 2730
BsrFI r'CCGG_y
Cuts at: 916 2429 3520 3701 916
Size: 1513 1091 181 2737
Fragments arranged by size:
2737 1513 1091 181
BssSI C'ACGA_G
Cuts at: 852 1186 3793 4501 852
Size: 334 2607 708 1873
Fragments arranged by size:
2607 1873 708 334
BstAPI GCAn_nnn'nTGC
Cuts at: 2899 2899
Size: 5522
BstBI TT'CG_AA
Cuts at: 3883 3883
Size: 5522
BstXI CCAn_nnnn'nTGG
Cuts at: 744 1479 744
Size: 735 4787
BstYI r'GATC_y
Cuts at: 1 324 603 1388 1418 2465 2547 3014 3037
Size: 323 279 785 30 1047 82 467 23
Cuts at: 3037 3372 3618 3924 3936 4022 5074 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 1047 785 467 449 335 323 306
279 246 86 82 30 23 12
BstZ17I GTA'TAC
Cuts at: 566 566
Size: 5522
BtgI C'CryG_G
Cuts at: 1048 1073 1163 1878 1908 2199 1048
Size: 25 90 715 30 291 4371
Fragments arranged by size:
4371 715 291 90 30 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 810 3472 3653 3816 810
Size: 2662 181 163 2516
Fragments arranged by size:
2662 2516 181 163
BtsI GCAGTG_nn'
Cuts at: 217 1504 2288 2765 217
Size: 1287 784 477 2974
Fragments arranged by size:
2974 1287 784 477
DraI TTT'AAA
Cuts at: 2521 2521
Size: 5522
DraIII CAC_nnn'GTG
Cuts at: 1947 1947
Size: 5522
DrdI GACnn_nn'nnGTC
Cuts at: 3228 4572 3228
Size: 1344 4178
EaeI y'GGCC_r
Cuts at: 1248 1905 2039 3107 3281 3672 3699 5044 1248
Size: 657 134 1068 174 391 27 1345 1726
Fragments arranged by size:
1726 1345 1068 657 391 174 134 27
EagI C'GGCC_G
Cuts at: 1905 3107 1905
Size: 1202 4320
EarI CTCTTCn'nnn_
Cuts at: 334 797 3545 3755 334
Size: 463 2748 210 2101
Fragments arranged by size:
2748 2101 463 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3730 4472 4618 5332 3730
Size: 742 146 714 3920
Fragments arranged by size:
3920 742 714 146
EcoICRI GAG'CTC
Cuts at: 903 2534 903
Size: 1631 3891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 491 631 1045 1521 1533 1774 1990 2029
Size: 176 140 414 476 12 241 216 39
Cuts at: 2029 2391 2445 3347 3779 4132 4794 315
Size: 362 54 902 432 353 662 1043
Fragments arranged by size:
1043 902 662 476 432 414 362 353
241 216 176 140 54 39 12
EcoO109I rG'GnC_Cy
Cuts at: 663 664 1440 1588 2661 663
Size: 1 776 148 1073 3524
Fragments arranged by size:
3524 1073 776 148 1
EcoRI G'AATT_C
Cuts at: 385 2526 385
Size: 2141 3381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1278 1440 3541 1278
Size: 162 2101 3259
Fragments arranged by size:
3259 2101 162
FspI TGC'GCA
Cuts at: 3303 3303
Size: 5522
HaeII r_GCGC'y
Cuts at: 134 1544 2956 3204 4434 5205 134
Size: 1410 1412 248 1230 771 451
Fragments arranged by size:
1412 1410 1230 771 451 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 563 595 1445 1477 2268 2300 3364 3394 3396
Size: 32 850 32 791 32 1064 30 2
Cuts at: 3396 3426 563
Size: 30 2659
Fragments arranged by size:
2659 1064 850 791 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2561 2561
Size: 5522
HindIII A'AGCT_T
Cuts at: 643 643
Size: 5522
KasI G'GCGC_C
Cuts at: 2952 3200 2952
Size: 248 5274
KpnI G_GTAC'C
Cuts at: 2542 2542
Size: 5522
MfeI C'AATT_G
Cuts at: 212 212
Size: 5522
MluI A'CGCG_T
Cuts at: 4955 4955
Size: 5522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4281 4465 4281
Size: 184 5338
MscI TGG'CCA
Cuts at: 3283 3283
Size: 5522
MslI CAynn'nnrTG
Cuts at: 742 834 1072 1075 1190 1312 3511 3638 742
Size: 92 238 3 115 122 2199 127 2626
Fragments arranged by size:
2626 2199 238 127 122 115 92 3
MspA1I CmG'CkG
Cuts at: 280 681 1916 2633 2947 3307 4091 4336 280
Size: 401 1235 717 314 360 784 245 1466
Fragments arranged by size:
1466 1235 784 717 401 360 314 245
NaeI GCC'GGC
Cuts at: 3703 3703
Size: 5522
NarI GG'CG_CC
Cuts at: 2953 3201 2953
Size: 248 5274
NcoI C'CATG_G
Cuts at: 1073 2199 1073
Size: 1126 4396
NgoMIV G'CCGG_C
Cuts at: 3701 3701
Size: 5522
NspI r_CATG'y
Cuts at: 724 1666 2575 3075 4678 724
Size: 942 909 500 1603 1568
Fragments arranged by size:
1603 1568 942 909 500
PasI CC'CwG_GG
Cuts at: 1281 1281
Size: 5522
PciI A'CATG_T
Cuts at: 720 1662 4674 720
Size: 942 3012 1568
Fragments arranged by size:
3012 1568 942
PflMI CCAn_nnn'nTGG
Cuts at: 1023 1560 1023
Size: 537 4985
PfoI T'CCnGG_A
Cuts at: 2468 2468
Size: 5522
PmeI GTTT'AAAC
Cuts at: 2521 2521
Size: 5522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 5490
PpuMI rG'GwC_Cy
Cuts at: 1440 1588 1440
Size: 148 5374
PspOMI G'GGCC_C
Cuts at: 663 663
Size: 5522
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1516 1548 1516
Size: 32 5490
PstI C_TGCA'G
Cuts at: 2569 3254 2569
Size: 685 4837
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5522
PvuII CAG'CTG
Cuts at: 681 2947 3307 681
Size: 2266 360 2896
Fragments arranged by size:
2896 2266 360
RsrII CG'GwC_CG
Cuts at: 3717 3717
Size: 5522
SacI G_AGCT'C
Cuts at: 905 2536 905
Size: 1631 3891
SalI G'TCGA_C
Cuts at: 2559 2559
Size: 5522
SapI GCTCTTCn'nnn_
Cuts at: 3545 3755 3545
Size: 210 5312
SbfI CC_TGCA'GG
Cuts at: 2569 2569
Size: 5522
ScaI AGT'ACT
Cuts at: 1063 5039 1063
Size: 3976 1546
SexAI A'CCwGG_T
Cuts at: 1514 1514
Size: 5522
SfcI C'TryA_G
Cuts at: 33 317 393 1464 2565 3250 4218 4409 33
Size: 284 76 1071 1101 685 968 191 1146
Fragments arranged by size:
1146 1101 1071 968 685 284 191 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 671 671
Size: 5522
SfoI GGC'GCC
Cuts at: 2954 3202 2954
Size: 248 5274
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
SmaI CCC'GGG
Cuts at: 1817 2544 1817
Size: 727 4795
SmlI C'TyrA_G
Cuts at: 1576 2677 3592 4306 4568 1576
Size: 1101 915 714 262 2530
Fragments arranged by size:
2530 1101 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5522
SphI G_CATG'C
Cuts at: 2575 3075 2575
Size: 500 5022
StuI AGG'CCT
Cuts at: 1204 1204
Size: 5522
StyI C'CwwG_G
Cuts at: 119 956 1073 1097 1241 1923 2199 2231 2656
Size: 837 117 24 144 682 276 32 425
Cuts at: 2656 3010 119
Size: 354 2631
Fragments arranged by size:
2631 837 682 425 354 276 144 117
32 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 249 1030 1700 1795 3105 3238 3697 3906 249
Size: 781 670 95 1310 133 459 209 1865
Fragments arranged by size:
1865 1310 781 670 459 209 133 95
TatI w'GTAC_w
Cuts at: 1061 2018 5037 1061
Size: 957 3019 1546
Fragments arranged by size:
3019 1546 957
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5039 5071 5039
Size: 32 5490
Tth111I GACn'n_nGTC
Cuts at: 3319 3319
Size: 5522
XbaI T'CTAG_A
Cuts at: 2553 2553
Size: 5522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1020 3017 1020
Size: 1997 3525
XmaI C'CCGG_G
Cuts at: 1815 2542 1815
Size: 727 4795
XmnI GAAnn'nnTTC
Cuts at: 2303 2303
Size: 5522
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AscI AvrII BaeI BbvCI
BfrBI BmtI BsiWI BsrGI BssHII BstEII Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
SacII SanDI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI