Restriction map for
pF1KB3098
(Circular) (Six-base) MAPSORT of: pF1KB3098.seq Check: 7677 from: 1 to: 5051
pF1KB3098 5051 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 991 2089 991
Size: 1098 3953
Acc65I G'GTAC_C
Cuts at: 2067 2067
Size: 5051
AclI AA'CG_TT
Cuts at: 4891 4891
Size: 5051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1460 1766 2876 3308 3661 4323 1460
Size: 306 1110 432 353 662 2188
Fragments arranged by size:
2188 1110 662 432 353 306
AflIII A'CryG_T
Cuts at: 2403 4203 4484 2403
Size: 1800 281 2970
Fragments arranged by size:
2970 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 180 203 2356 3794 180
Size: 23 2153 1438 1437
Fragments arranged by size:
2153 1438 1437 23
ApaI G_GGCC'C
Cuts at: 326 326
Size: 5051
ApaLI G'TGCA_C
Cuts at: 3889 3889
Size: 5051
ApoI r'AATT_y
Cuts at: 778 1000 1033 1729 1878 2055 4404 4660 778
Size: 222 33 696 149 177 2349 256 1169
Fragments arranged by size:
2349 1169 696 256 222 177 149 33
AscI GG'CGCG_CC
Cuts at: 574 574
Size: 5051
AseI AT'TA_AT
Cuts at: 20 812 20
Size: 792 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
AvaI C'yCGr_G
Cuts at: 319 325 343 405 618 701 2071 319
Size: 6 18 62 213 83 1370 3299
Fragments arranged by size:
3299 1370 213 83 62 18 6
BamHI G'GATC_C
Cuts at: 2076 2076
Size: 5051
BanI G'GyrC_C
Cuts at: 397 622 2067 2481 2729 2764 397
Size: 225 1445 414 248 35 2684
Fragments arranged by size:
2684 1445 414 248 225 35
BanII G_rGCy'C
Cuts at: 281 310 320 326 1117 2065 3095 281
Size: 29 10 6 791 948 1030 2237
Fragments arranged by size:
2237 1030 948 791 29 10 6
BbeI G_GCGC'C
Cuts at: 401 2485 2733 401
Size: 2084 248 2719
Fragments arranged by size:
2719 2084 248
BbsI GAAGACnn'nnnn_
Cuts at: 908 1782 908
Size: 874 4177
BbvCI CC'TCA_GC
Cuts at: 1931 1931
Size: 5051
BciVI GTATCCnnnnn_n'
Cuts at: 747 1700 2949 4005 5051 747
Size: 953 1249 1056 1046 747
Fragments arranged by size:
1249 1056 1046 953 747
BclI T'GATC_A
Cuts at: 1215 2571 1215
Size: 1356 3695
BfrBI ATG'CAT
Cuts at: 1076 1076
Size: 5051
BglI GCCn_nnn'nGGC
Cuts at: 157 584 157
Size: 427 4624
BglII A'GATC_T
Cuts at: 2566 4603 2566
Size: 2037 3014
BlpI GC'TnA_GC
Cuts at: 2163 2163
Size: 5051
Bme1580I G_kGCm'C
Cuts at: 326 625 2676 2769 3893 326
Size: 299 2051 93 1124 1484
Fragments arranged by size:
2051 1484 1124 299 93
BmrI ACTGGGnnnn_n'
Cuts at: 2263 2344 2679 4823 2263
Size: 81 335 2144 2491
Fragments arranged by size:
2491 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 300 459 3418 300
Size: 159 2959 1933
Fragments arranged by size:
2959 1933 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1049 1503 394
Size: 655 454 3942
Fragments arranged by size:
3942 655 454
Bpu10I CC'TnA_GC
Cuts at: 118 1931 118
Size: 1813 3238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 785 1368 1885 2227 3106 3820 4118 785
Size: 583 517 342 879 714 298 1718
Fragments arranged by size:
1718 879 714 583 517 342 298
BsaI GGTCTCn'nnnn_
Cuts at: 4755 4755
Size: 5051
BsaAI yAC'GTr
Cuts at: 2404 2404
Size: 5051
BsaBI GATnn'nnATC
Cuts at: 6 2565 4602 6
Size: 2559 2037 455
Fragments arranged by size:
2559 2037 455
BsaHI Gr'CG_yC
Cuts at: 398 2482 2730 3432 4928 398
Size: 2084 248 702 1496 521
Fragments arranged by size:
2084 1496 702 521 248
BsaWI w'CCGG_w
Cuts at: 825 1955 2240 2761 3439 3850 3997 4271 825
Size: 1130 285 521 678 411 147 274 1605
Fragments arranged by size:
1605 1130 678 521 411 285 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 370 400 370
Size: 30 5021
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 226 238 241 244 247 391 459 466
Size: 3 12 3 3 3 144 68 7
Cuts at: 466 469 788 1549 223
Size: 3 319 761 3725
Fragments arranged by size:
3725 761 319 144 68 12 7 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2476 3234 3899 4307 2476
Size: 758 665 408 3220
Fragments arranged by size:
3220 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 1856 147
Size: 1709 3342
BsiEI CG_ry'CG
Cuts at: 104 610 2593 2639 3869 104
Size: 506 1983 46 1230 1286
Fragments arranged by size:
1983 1286 1230 506 46
BsiHKAI G_wGCw'C
Cuts at: 1944 2065 2843 3033 3893 1944
Size: 121 778 190 860 3102
Fragments arranged by size:
3102 860 778 190 121
BsmI GAATG_Cn'
Cuts at: 1173 1803 1866 1173
Size: 630 63 4358
Fragments arranged by size:
4358 630 63
BsmBI CGTCTCn'nnnn_
Cuts at: 1442 1442
Size: 5051
Bsp1286I G_dGCh'C
Cuts at: 281 310 320 326 625 1117 1944 2065 2676
Size: 29 10 6 299 492 827 121 611
Cuts at: 2676 2769 2843 3033 3095 3893 281
Size: 93 74 190 62 798 1439
Fragments arranged by size:
1439 827 798 611 492 299 190 121
93 74 62 29 10 6
BspEI T'CCGG_A
Cuts at: 825 825
Size: 5051
BspHI T'CATG_A
Cuts at: 1005 1289 3483 1005
Size: 284 2194 2573
Fragments arranged by size:
2573 2194 284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 954 2101 2315 2617 2998 954
Size: 1147 214 302 381 3007
Fragments arranged by size:
3007 1147 381 302 214
BsrBI CCG'CTC
Cuts at: 50 338 599 2024 3343 3397 4881 5046 50
Size: 288 261 1425 1319 54 1484 165 55
Fragments arranged by size:
1484 1425 1319 288 261 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1793 2963 1793
Size: 1170 3881
BsrFI r'CCGG_y
Cuts at: 348 477 489 525 3049 3230 348
Size: 129 12 36 2524 181 2169
Fragments arranged by size:
2524 2169 181 129 36 12
BssHII G'CGCG_C
Cuts at: 574 574
Size: 5051
BssSI C'ACGA_G
Cuts at: 444 3322 4030 444
Size: 2878 708 1465
Fragments arranged by size:
2878 1465 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 2428 1924
Size: 504 4547
BstBI TT'CG_AA
Cuts at: 3412 3412
Size: 5051
BstYI r'GATC_y
Cuts at: 1 1320 1514 1764 2076 2543 2566 2901 3147
Size: 1319 194 250 312 467 23 335 246
Cuts at: 3147 3453 3465 3551 4603 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1319 1052 467 449 335 312 306 250
246 194 86 23 12
BstZ17I GTA'TAC
Cuts at: 992 992
Size: 5051
Bsu36I CC'TnA_GG
Cuts at: 849 849
Size: 5051
BtgI C'CryG_G
Cuts at: 311 559 604 311
Size: 248 45 4758
Fragments arranged by size:
4758 248 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3001 3182 3345 3001
Size: 181 163 4707
Fragments arranged by size:
4707 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 2294 1166
Size: 1128 3923
DraI TTT'AAA
Cuts at: 1302 1485 2050 1302
Size: 183 565 4303
Fragments arranged by size:
4303 565 183
DrdI GACnn_nn'nnGTC
Cuts at: 860 2757 4101 860
Size: 1897 1344 1810
Fragments arranged by size:
1897 1810 1344
EaeI y'GGCC_r
Cuts at: 243 607 1338 1961 2636 2810 3201 3228 4573
Size: 364 731 623 675 174 391 27 1345
Cuts at: 4573 243
Size: 721
Fragments arranged by size:
1345 731 721 675 623 391 364 174
27
EagI C'GGCC_G
Cuts at: 607 2636 607
Size: 2029 3022
EarI CTCTTCn'nnn_
Cuts at: 215 664 751 1054 1658 1951 3074 3284 215
Size: 449 87 303 604 293 1123 210 1982
Fragments arranged by size:
1982 1123 604 449 303 293 210 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 448 3259 4001 4147 4861 448
Size: 2811 742 146 714 638
Fragments arranged by size:
2811 742 714 638 146
EcoICRI GAG'CTC
Cuts at: 2063 2063
Size: 5051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1049 1460 1503 1766 2876 3308 3661 4323
Size: 655 411 43 263 1110 432 353 662
Cuts at: 4323 394
Size: 1122
Fragments arranged by size:
1122 1110 662 655 432 411 353 263
43
EcoO109I rG'GnC_Cy
Cuts at: 381 2190 381
Size: 1809 3242
EcoRI G'AATT_C
Cuts at: 2055 2055
Size: 5051
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 201 3070 201
Size: 2869 2182
FseI GG_CCGG'CC
Cuts at: 352 352
Size: 5051
FspI TGC'GCA
Cuts at: 2832 2832
Size: 5051
HaeII r_GCGC'y
Cuts at: 333 401 2485 2733 3963 4734 333
Size: 68 2084 248 1230 771 650
Fragments arranged by size:
2084 1230 771 650 248 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 703 735 1450 1482 1506 1538 2893 2923 2925
Size: 32 715 32 24 32 1355 30 2
Cuts at: 2925 2955 703
Size: 30 2799
Fragments arranged by size:
2799 1355 715 32 32 32 30 30
24 2
HincII GTy'rAC
Cuts at: 2090 2090
Size: 5051
KasI G'GCGC_C
Cuts at: 397 2481 2729 397
Size: 2084 248 2719
Fragments arranged by size:
2719 2084 248
KpnI G_GTAC'C
Cuts at: 2071 2071
Size: 5051
MluI A'CGCG_T
Cuts at: 4484 4484
Size: 5051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1493 3810 3994 1493
Size: 2317 184 2550
Fragments arranged by size:
2550 2317 184
MscI TGG'CCA
Cuts at: 1963 2812 1963
Size: 849 4202
MslI CAynn'nnrTG
Cuts at: 1178 3040 3167 1178
Size: 1862 127 3062
Fragments arranged by size:
3062 1862 127
MspA1I CmG'CkG
Cuts at: 242 291 561 606 1930 2162 2476 2836 3620
Size: 49 270 45 1324 232 314 360 784
Cuts at: 3620 3865 242
Size: 245 1428
Fragments arranged by size:
1428 1324 784 360 314 270 245 232
49 45
NaeI GCC'GGC
Cuts at: 350 491 3232 350
Size: 141 2741 2169
Fragments arranged by size:
2741 2169 141
NarI GG'CG_CC
Cuts at: 398 2482 2730 398
Size: 2084 248 2719
Fragments arranged by size:
2719 2084 248
NdeI CA'TA_TG
Cuts at: 1220 1220
Size: 5051
NgoMIV G'CCGG_C
Cuts at: 348 489 3230 348
Size: 141 2741 2169
Fragments arranged by size:
2741 2169 141
NsiI A_TGCA'T
Cuts at: 1078 1078
Size: 5051
NspI r_CATG'y
Cuts at: 1507 2104 2604 4207 1507
Size: 597 500 1603 2351
Fragments arranged by size:
2351 1603 597 500
PciI A'CATG_T
Cuts at: 4203 4203
Size: 5051
PmeI GTTT'AAAC
Cuts at: 2050 2050
Size: 5051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 5019
PpuMI rG'GwC_Cy
Cuts at: 381 381
Size: 5051
PspOMI G'GGCC_C
Cuts at: 322 322
Size: 5051
PstI C_TGCA'G
Cuts at: 199 1929 2098 2783 199
Size: 1730 169 685 2467
Fragments arranged by size:
2467 1730 685 169
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5051
PvuII CAG'CTG
Cuts at: 1930 2476 2836 1930
Size: 546 360 4145
Fragments arranged by size:
4145 546 360
RsrII CG'GwC_CG
Cuts at: 3246 3246
Size: 5051
SacI G_AGCT'C
Cuts at: 2065 2065
Size: 5051
SacII CC_GC'GG
Cuts at: 562 607 562
Size: 45 5006
SalI G'TCGA_C
Cuts at: 2088 2088
Size: 5051
SapI GCTCTTCn'nnn_
Cuts at: 3074 3284 3074
Size: 210 4841
SbfI CC_TGCA'GG
Cuts at: 2098 2098
Size: 5051
ScaI AGT'ACT
Cuts at: 4568 4568
Size: 5051
SfcI C'TryA_G
Cuts at: 33 195 1281 1925 2094 2779 3747 3938 33
Size: 162 1086 644 169 685 968 191 1146
Fragments arranged by size:
1146 1086 968 685 644 191 169 162
SfoI GGC'GCC
Cuts at: 399 2483 2731 399
Size: 2084 248 2719
Fragments arranged by size:
2719 2084 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
SgrAI Cr'CCGG_yG
Cuts at: 489 525 489
Size: 36 5015
SmaI CCC'GGG
Cuts at: 321 345 407 620 2073 321
Size: 24 62 213 1453 3299
Fragments arranged by size:
3299 1453 213 62 24
SmlI C'TyrA_G
Cuts at: 800 1347 1900 2206 3121 3835 4097 800
Size: 547 553 306 915 714 262 1754
Fragments arranged by size:
1754 915 714 553 547 306 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5051
SphI G_CATG'C
Cuts at: 2104 2604 2104
Size: 500 4551
SrfI GCCC'GGGC
Cuts at: 321 345 407 321
Size: 24 62 4965
Fragments arranged by size:
4965 62 24
SspI AAT'ATT
Cuts at: 810 1240 1401 810
Size: 430 161 4460
Fragments arranged by size:
4460 430 161
StyI C'CwwG_G
Cuts at: 2185 2539 2185
Size: 354 4697
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1993 2634 2767 3226 3435 1993
Size: 641 133 459 209 3609
Fragments arranged by size:
3609 641 459 209 133
TatI w'GTAC_w
Cuts at: 4566 4566
Size: 5051
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4568 4600 4568
Size: 32 5019
Tth111I GACn'n_nGTC
Cuts at: 857 2848 857
Size: 1991 3060
XbaI T'CTAG_A
Cuts at: 1825 2082 1825
Size: 257 4794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2546 2546
Size: 5051
XmaI C'CCGG_G
Cuts at: 319 343 405 618 2071 319
Size: 24 62 213 1453 3299
Fragments arranged by size:
3299 1453 213 62 24
XmnI GAAnn'nnTTC
Cuts at: 711 1652 1779 711
Size: 941 127 3983
Fragments arranged by size:
3983 941 127
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AvrII BaeI BcgI BmgBI BmtI BsiWI BsrGI
BstEII BstXI ClaI CspCI DraIII EcoNI EcoRV FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SnaBI StuI SwaI XhoI ZraI