Restriction map for
pF1KB3093
(Circular) (Six-base) MAPSORT of: pF1KB3093.seq Check: 3585 from: 1 to: 5348
pF1KB3093 5348 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 211 211
Size: 5348
AatII G_ACGT'C
Cuts at: 1322 1322
Size: 5348
AccI GT'mk_AC
Cuts at: 2386 2386
Size: 5348
Acc65I G'GTAC_C
Cuts at: 2364 2364
Size: 5348
AclI AA'CG_TT
Cuts at: 5188 5188
Size: 5348
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1306 3173 3605 4352 4620 1306
Size: 1867 432 747 268 2034
Fragments arranged by size:
2034 1867 747 432 268
AflIII A'CryG_T
Cuts at: 2700 3804 4781 2700
Size: 1104 977 3267
Fragments arranged by size:
3267 1104 977
AgeI A'CCGG_T
Cuts at: 3736 3736
Size: 5348
AhdI GACnn_n'nnGTC
Cuts at: 1100 1100
Size: 5348
AleI CACnn'nnGTG
Cuts at: 205 421 205
Size: 216 5132
AlwNI CAG_nnn'CTG
Cuts at: 453 990 1176 1884 2653 4220 453
Size: 537 186 708 769 1567 1581
Fragments arranged by size:
1581 1567 769 708 537 186
ApaI G_GGCC'C
Cuts at: 642 1043 642
Size: 401 4947
ApaLI G'TGCA_C
Cuts at: 4118 4118
Size: 5348
ApoI r'AATT_y
Cuts at: 2352 4701 4957 2352
Size: 2349 256 2743
Fragments arranged by size:
2743 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5348
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5348
AvaI C'yCGr_G
Cuts at: 177 189 541 1820 2368 177
Size: 12 352 1279 548 3157
Fragments arranged by size:
3157 1279 548 352 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 165 198 165
Size: 33 5315
BamHI G'GATC_C
Cuts at: 2373 2373
Size: 5348
BanI G'GyrC_C
Cuts at: 193 257 300 883 927 2364 2778 3026 3061
Size: 64 43 583 44 1437 414 248 35
Cuts at: 3061 193
Size: 2480
Fragments arranged by size:
2480 1437 583 414 248 64 44 43
35
BanII G_rGCy'C
Cuts at: 548 642 652 1043 2111 2183 2362 3392 548
Size: 94 10 391 1068 72 179 1030 2504
Fragments arranged by size:
2504 1068 1030 391 179 94 72 10
BbeI G_GCGC'C
Cuts at: 197 261 887 2782 3030 197
Size: 64 626 1895 248 2515
Fragments arranged by size:
2515 1895 626 248 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2011 2045 2011
Size: 34 5314
BciVI GTATCCnnnnn_n'
Cuts at: 193 3246 4007 5348 193
Size: 3053 761 1341 193
Fragments arranged by size:
3053 1341 761 193
BclI T'GATC_A
Cuts at: 1019 1619 2868 1019
Size: 600 1249 3499
Fragments arranged by size:
3499 1249 600
BglI GCCn_nnn'nGGC
Cuts at: 523 892 1519 2149 523
Size: 369 627 630 3722
Fragments arranged by size:
3722 630 627 369
BglII A'GATC_T
Cuts at: 1844 2863 4900 1844
Size: 1019 2037 2292
Fragments arranged by size:
2292 2037 1019
BlpI GC'TnA_GC
Cuts at: 811 2460 811
Size: 1649 3699
Bme1580I G_kGCm'C
Cuts at: 212 305 379 642 932 1043 1348 1642 2029
Size: 93 74 263 290 111 305 294 387
Cuts at: 2029 2237 2973 3066 4122 212
Size: 208 736 93 1056 1438
Fragments arranged by size:
1438 1056 736 387 305 294 290 263
208 111 93 93 74
BmrI ACTGGGnnnn_n'
Cuts at: 2560 2641 2976 5120 2560
Size: 81 335 2144 2788
Fragments arranged by size:
2788 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 417 2001 3715 417
Size: 1584 1714 2050
Fragments arranged by size:
2050 1714 1584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1294 2208 2269 343
Size: 951 914 61 3422
Fragments arranged by size:
3422 951 914 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 460 1096 2524 3403 3895 4193 256
Size: 204 636 1428 879 492 298 1411
Fragments arranged by size:
1428 1411 879 636 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 5052 5052
Size: 5348
BsaAI yAC'GTr
Cuts at: 1731 2701 1731
Size: 970 4378
BsaBI GATnn'nnATC
Cuts at: 6 2170 2862 4899 6
Size: 2164 692 2037 455
Fragments arranged by size:
2164 2037 692 455
BsaHI Gr'CG_yC
Cuts at: 194 258 628 884 1319 1964 2779 3027 3729
Size: 64 370 256 435 645 815 248 702
Cuts at: 3729 5225 194
Size: 1496 317
Fragments arranged by size:
1496 815 702 645 435 370 317 256
248 64
BsaWI w'CCGG_w
Cuts at: 1798 2087 2537 3058 3736 4010 4157 4568 1798
Size: 289 450 521 678 274 147 411 2578
Fragments arranged by size:
2578 678 521 450 411 289 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 358 388 727 757 358
Size: 30 339 30 4949
Fragments arranged by size:
4949 339 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 1792 253
Size: 1539 3809
BseYI C'CCAG_C
Cuts at: 307 467 656 1132 1554 1641 1721 2773 3531
Size: 160 189 476 422 87 80 1052 758
Cuts at: 3531 4108 4604 307
Size: 577 496 1051
Fragments arranged by size:
1052 1051 758 577 496 476 422 189
160 87 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 702 718 841 1075 1234 1779 1792 1897 1993
Size: 16 123 234 159 545 13 105 96
Cuts at: 1993 702
Size: 4057
Fragments arranged by size:
4057 545 234 159 123 105 96 16
13
BsiEI CG_ry'CG
Cuts at: 104 1961 2890 2936 4144 104
Size: 1857 929 46 1208 1308
Fragments arranged by size:
1857 1308 1208 929 46
BsiHKAI G_wGCw'C
Cuts at: 548 1112 1806 2111 2362 3140 3330 4122 548
Size: 564 694 305 251 778 190 792 1774
Fragments arranged by size:
1774 792 778 694 564 305 251 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1543 1543
Size: 5348
Bsp1286I G_dGCh'C
Cuts at: 212 305 379 548 642 652 932 1043 1112
Size: 93 74 169 94 10 280 111 69
Cuts at: 1112 1348 1642 1806 2029 2111 2183 2237 2362
Size: 236 294 164 223 82 72 54 125
Cuts at: 2362 2973 3066 3140 3330 3392 4122 212
Size: 611 93 74 190 62 730 1438
Fragments arranged by size:
1438 730 611 294 280 236 223 190
169 164 125 111 94 93 93 82
74 74 72 69 62 54 10
BspEI T'CCGG_A
Cuts at: 4568 4568
Size: 5348
BspHI T'CATG_A
Cuts at: 4524 4524
Size: 5348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 211 452 872 2398 2612 2914 3295 211
Size: 241 420 1526 214 302 381 2264
Fragments arranged by size:
2264 1526 420 381 302 241 214
BsrBI CCG'CTC
Cuts at: 50 3640 3694 5178 5343 50
Size: 3590 54 1484 165 55
Fragments arranged by size:
3590 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3260 3260
Size: 5348
BsrFI r'CCGG_y
Cuts at: 3346 3527 3736 3346
Size: 181 209 4958
Fragments arranged by size:
4958 209 181
BssSI C'ACGA_G
Cuts at: 1051 3619 3977 1051
Size: 2568 358 2422
Fragments arranged by size:
2568 2422 358
BstAPI GCAn_nnn'nTGC
Cuts at: 864 1062 2725 864
Size: 198 1663 3487
Fragments arranged by size:
3487 1663 198
BstBI TT'CG_AA
Cuts at: 3709 3709
Size: 5348
BstEII G'GTnAC_C
Cuts at: 582 582
Size: 5348
BstXI CCAn_nnnn'nTGG
Cuts at: 423 702 960 423
Size: 279 258 4811
Fragments arranged by size:
4811 279 258
BstYI r'GATC_y
Cuts at: 1 1844 2373 2840 2863 3198 3444 4456 4542
Size: 1843 529 467 23 335 246 1012 86
Cuts at: 4542 4554 4900 1
Size: 12 346 449
Fragments arranged by size:
1843 1012 529 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1508 2305 1508
Size: 797 4551
BtgI C'CryG_G
Cuts at: 107 1340 2102 107
Size: 1233 762 3353
Fragments arranged by size:
3353 1233 762
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2243 3298 3479 3642 2243
Size: 1055 181 163 3949
Fragments arranged by size:
3949 1055 181 163
BtsI GCAGTG_nn'
Cuts at: 1804 2027 2321 2591 1804
Size: 223 294 270 4561
Fragments arranged by size:
4561 294 270 223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 765 800 765
Size: 35 5313
DraI TTT'AAA
Cuts at: 2347 2347
Size: 5348
DrdI GACnn_nn'nnGTC
Cuts at: 1324 3054 3912 1324
Size: 1730 858 2760
Fragments arranged by size:
2760 1730 858
EaeI y'GGCC_r
Cuts at: 413 455 482 524 593 665 893 905 962
Size: 42 27 42 69 72 228 12 57
Cuts at: 962 1028 1253 1591 1958 2339 2933 3107 3498
Size: 66 225 338 367 381 594 174 391
Cuts at: 3498 3525 4870 413
Size: 27 1345 891
Fragments arranged by size:
1345 891 594 391 381 367 338 228
225 174 72 69 66 57 42 42
27 27 12
EagI C'GGCC_G
Cuts at: 1958 2933 1958
Size: 975 4373
EarI CTCTTCn'nnn_
Cuts at: 2128 3371 3581 2128
Size: 1243 210 3895
Fragments arranged by size:
3895 1243 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3556 3866 4012 5158 3556
Size: 310 146 1146 3746
Fragments arranged by size:
3746 1146 310 146
EcoICRI GAG'CTC
Cuts at: 546 2109 2360 546
Size: 1563 251 3534
Fragments arranged by size:
3534 1563 251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1294 1306 2208 2269 3173 3605 4352 4620
Size: 951 12 902 61 904 432 747 268
Cuts at: 4620 343
Size: 1071
Fragments arranged by size:
1071 951 904 902 747 432 268 61
12
EcoNI CCTnn'n_nnAGG
Cuts at: 874 874
Size: 5348
EcoO109I rG'GnC_Cy
Cuts at: 570 639 1040 1047 1173 1269 1824 1982 2309
Size: 69 401 7 126 96 555 158 327
Cuts at: 2309 2487 570
Size: 178 3431
Fragments arranged by size:
3431 555 401 327 178 158 126 96
69 7
EcoRI G'AATT_C
Cuts at: 2352 2352
Size: 5348
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3367 3367
Size: 5348
FspI TGC'GCA
Cuts at: 1237 3129 1237
Size: 1892 3456
HaeII r_GCGC'y
Cuts at: 197 261 887 1728 2782 3030 4052 5031 197
Size: 64 626 841 1054 248 1022 979 514
Fragments arranged by size:
1054 1022 979 841 626 514 248 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1086 1118 1611 1643 3190 3220 3222 3252 1086
Size: 32 493 32 1547 30 2 30 3182
Fragments arranged by size:
3182 1547 493 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1323 2387 1323
Size: 1064 4284
KasI G'GCGC_C
Cuts at: 193 257 883 2778 3026 193
Size: 64 626 1895 248 2515
Fragments arranged by size:
2515 1895 626 248 64
KpnI G_GTAC'C
Cuts at: 2368 2368
Size: 5348
MluI A'CGCG_T
Cuts at: 4781 4781
Size: 5348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1379 1716 4019 4203 1379
Size: 337 2303 184 2524
Fragments arranged by size:
2524 2303 337 184
MscI TGG'CCA
Cuts at: 415 526 964 1030 1255 1593 3109 415
Size: 111 438 66 225 338 1516 2654
Fragments arranged by size:
2654 1516 438 338 225 111 66
MslI CAynn'nnrTG
Cuts at: 112 205 421 601 1715 2263 3337 3464 112
Size: 93 216 180 1114 548 1074 127 1996
Fragments arranged by size:
1996 1114 1074 548 216 180 127 93
MspA1I CmG'CkG
Cuts at: 471 612 660 670 716 1305 1406 1659 1672
Size: 141 48 10 46 589 101 253 13
Cuts at: 1672 2247 2459 2773 3133 4146 4391 471
Size: 575 212 314 360 1013 245 1428
Fragments arranged by size:
1428 1013 589 575 360 314 253 245
212 141 101 48 46 13 10
NaeI GCC'GGC
Cuts at: 3529 3529
Size: 5348
NarI GG'CG_CC
Cuts at: 194 258 884 2779 3027 194
Size: 64 626 1895 248 2515
Fragments arranged by size:
2515 1895 626 248 64
NcoI C'CATG_G
Cuts at: 107 107
Size: 5348
NgoMIV G'CCGG_C
Cuts at: 3527 3527
Size: 5348
NspI r_CATG'y
Cuts at: 810 1367 2337 2401 2901 3808 810
Size: 557 970 64 500 907 2350
Fragments arranged by size:
2350 970 907 557 500 64
PasI CC'CwG_GG
Cuts at: 294 1176 294
Size: 882 4466
PciI A'CATG_T
Cuts at: 3804 3804
Size: 5348
PflMI CCAn_nnn'nTGG
Cuts at: 663 1589 1698 1914 2337 663
Size: 926 109 216 423 3674
Fragments arranged by size:
3674 926 423 216 109
PmeI GTTT'AAAC
Cuts at: 2347 2347
Size: 5348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4487 4519 4487
Size: 32 5316
PpuMI rG'GwC_Cy
Cuts at: 570 1824 570
Size: 1254 4094
PspOMI G'GGCC_C
Cuts at: 638 1039 638
Size: 401 4947
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2068 2100 2068
Size: 32 5316
PstI C_TGCA'G
Cuts at: 449 986 1664 1740 2328 2395 3080 449
Size: 537 678 76 588 67 685 2717
Fragments arranged by size:
2717 685 678 588 537 76 67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5348
PvuII CAG'CTG
Cuts at: 471 612 660 1305 2773 3133 471
Size: 141 48 645 1468 360 2686
Fragments arranged by size:
2686 1468 645 360 141 48
RsrII CG'GwC_CG
Cuts at: 3543 3543
Size: 5348
SacI G_AGCT'C
Cuts at: 548 2111 2362 548
Size: 1563 251 3534
Fragments arranged by size:
3534 1563 251
SalI G'TCGA_C
Cuts at: 2385 2385
Size: 5348
SanDI GG'GwC_CC
Cuts at: 1824 1824
Size: 5348
SapI GCTCTTCn'nnn_
Cuts at: 3371 3581 3371
Size: 210 5138
SbfI CC_TGCA'GG
Cuts at: 2395 2395
Size: 5348
ScaI AGT'ACT
Cuts at: 4865 4865
Size: 5348
SfcI C'TryA_G
Cuts at: 33 445 982 1071 1660 1736 2292 2324 2391
Size: 412 537 89 589 76 556 32 67
Cuts at: 2391 3076 4069 4260 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 589 556 537 412 191
89 76 67 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 1519 1519
Size: 5348
SfoI GGC'GCC
Cuts at: 195 259 885 2780 3028 195
Size: 64 626 1895 248 2515
Fragments arranged by size:
2515 1895 626 248 64
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5348
SmaI CCC'GGG
Cuts at: 1822 2370 1822
Size: 548 4800
SmlI C'TyrA_G
Cuts at: 271 475 1111 2503 3418 3910 4172 271
Size: 204 636 1392 915 492 262 1447
Fragments arranged by size:
1447 1392 915 636 492 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5348
SphI G_CATG'C
Cuts at: 810 2401 2901 810
Size: 1591 500 3257
Fragments arranged by size:
3257 1591 500
StuI AGG'CCT
Cuts at: 250 250
Size: 5348
StyI C'CwwG_G
Cuts at: 107 312 587 2482 2836 107
Size: 205 275 1895 354 2619
Fragments arranged by size:
2619 1895 354 275 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 307 433 819 2931 3064 3523 3732 307
Size: 126 386 2112 133 459 209 1923
Fragments arranged by size:
2112 1923 459 386 209 133 126
TatI w'GTAC_w
Cuts at: 263 1886 2219 4863 263
Size: 1623 333 2644 748
Fragments arranged by size:
2644 1623 748 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 358 390 727 759 1796 1828 4865 4897 358
Size: 32 337 32 1037 32 3037 32 809
Fragments arranged by size:
3037 1037 809 337 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3145 3145
Size: 5348
XbaI T'CTAG_A
Cuts at: 2379 2379
Size: 5348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1836 2843 1836
Size: 1007 4341
XmaI C'CCGG_G
Cuts at: 1820 2368 1820
Size: 548 4800
ZraI GAC'GTC
Cuts at: 1320 1320
Size: 5348
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI AscI AvrII BbsI BbvCI
BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsrGI BssHII
BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspXI SacII SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI