Restriction map for
pF1KB3090
(Circular) (Six-base) MAPSORT of: pF1KB3090.seq Check: 4161 from: 1 to: 4967
pF1KB3090 4967 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 259 2005 259
Size: 1746 3221
Acc65I G'GTAC_C
Cuts at: 1983 1983
Size: 4967
AclI AA'CG_TT
Cuts at: 4807 4807
Size: 4967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 1178 1244 1439 1478 2792 3224 3577 4239
Size: 501 66 195 39 1314 432 353 662
Cuts at: 4239 677
Size: 1405
Fragments arranged by size:
1405 1314 662 501 432 353 195 66
39
AflIII A'CryG_T
Cuts at: 1525 2319 4119 4400 1525
Size: 794 1800 281 2092
Fragments arranged by size:
2092 1800 794 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 134 929 134
Size: 795 4172
AlwNI CAG_nnn'CTG
Cuts at: 453 794 2272 3710 453
Size: 341 1478 1438 1710
Fragments arranged by size:
1710 1478 1438 341
ApaLI G'TGCA_C
Cuts at: 3805 3805
Size: 4967
ApoI r'AATT_y
Cuts at: 861 1971 4320 4576 861
Size: 1110 2349 256 1252
Fragments arranged by size:
2349 1252 1110 256
AseI AT'TA_AT
Cuts at: 20 1486 1571 20
Size: 1466 85 3416
Fragments arranged by size:
3416 1466 85
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4967
AvaI C'yCGr_G
Cuts at: 1987 1987
Size: 4967
BamHI G'GATC_C
Cuts at: 472 1992 472
Size: 1520 3447
BanI G'GyrC_C
Cuts at: 285 1983 2397 2645 2680 285
Size: 1698 414 248 35 2572
Fragments arranged by size:
2572 1698 414 248 35
BanII G_rGCy'C
Cuts at: 1981 3011 1981
Size: 1030 3937
BbeI G_GCGC'C
Cuts at: 2401 2649 2401
Size: 248 4719
BbsI GAAGACnn'nnnn_
Cuts at: 614 614
Size: 4967
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4933
BciVI GTATCCnnnnn_n'
Cuts at: 2865 3921 4967 2865
Size: 1056 1046 2865
Fragments arranged by size:
2865 1056 1046
BclI T'GATC_A
Cuts at: 2487 2487
Size: 4967
BglII A'GATC_T
Cuts at: 2482 4519 2482
Size: 2037 2930
BlpI GC'TnA_GC
Cuts at: 2079 2079
Size: 4967
Bme1580I G_kGCm'C
Cuts at: 1896 2592 2685 3809 1896
Size: 696 93 1124 3054
Fragments arranged by size:
3054 1124 696 93
BmrI ACTGGGnnnn_n'
Cuts at: 804 2179 2260 2595 4739 804
Size: 1375 81 335 2144 1032
Fragments arranged by size:
2144 1375 1032 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 326 3334 326
Size: 3008 1959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 770 1082 1226 1268 1388 552
Size: 218 312 144 42 120 4131
Fragments arranged by size:
4131 312 218 144 120 42
Bpu10I CC'TnA_GC
Cuts at: 968 968
Size: 4967
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1065 2143 3022 3736 4034 1065
Size: 1078 879 714 298 1998
Fragments arranged by size:
1998 1078 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1319 4671 1319
Size: 3352 1615
BsaAI yAC'GTr
Cuts at: 2320 2320
Size: 4967
BsaBI GATnn'nnATC
Cuts at: 6 2481 4518 6
Size: 2475 2037 455
Fragments arranged by size:
2475 2037 455
BsaHI Gr'CG_yC
Cuts at: 2398 2646 3348 4844 2398
Size: 248 702 1496 2521
Fragments arranged by size:
2521 1496 702 248
BsaWI w'CCGG_w
Cuts at: 827 2156 2677 3355 3766 3913 4187 827
Size: 1329 521 678 411 147 274 1607
Fragments arranged by size:
1607 1329 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 743 773 743
Size: 30 4937
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1453 1809 1453
Size: 356 4611
BseYI C'CCAG_C
Cuts at: 2392 3150 3815 4223 2392
Size: 758 665 408 3136
Fragments arranged by size:
3136 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2509 2555 3785 104
Size: 2405 46 1230 1286
Fragments arranged by size:
2405 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1981 2759 2949 3809 1981
Size: 778 190 860 3139
Fragments arranged by size:
3139 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 497 497
Size: 4967
Bsp1286I G_dGCh'C
Cuts at: 1896 1981 2592 2685 2759 2949 3011 3809 1896
Size: 85 611 93 74 190 62 798 3054
Fragments arranged by size:
3054 798 611 190 93 85 74 62
BspHI T'CATG_A
Cuts at: 1274 3399 1274
Size: 2125 2842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2017 2231 2533 2914 2017
Size: 214 302 381 4070
Fragments arranged by size:
4070 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3259 3313 4797 4962 50
Size: 3209 54 1484 165 55
Fragments arranged by size:
3209 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 653 1014 2879 653
Size: 361 1865 2741
Fragments arranged by size:
2741 1865 361
BsrFI r'CCGG_y
Cuts at: 2965 3146 2965
Size: 181 4786
BssSI C'ACGA_G
Cuts at: 311 3238 3946 311
Size: 2927 708 1332
Fragments arranged by size:
2927 1332 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2344 2344
Size: 4967
BstBI TT'CG_AA
Cuts at: 3328 3328
Size: 4967
BstYI r'GATC_y
Cuts at: 1 472 1992 2459 2482 2817 3063 3369 3381
Size: 471 1520 467 23 335 246 306 12
Cuts at: 3381 3467 4519 1
Size: 86 1052 449
Fragments arranged by size:
1520 1052 471 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 1072 1072
Size: 4967
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2917 3098 3261 2917
Size: 181 163 4623
Fragments arranged by size:
4623 181 163
BtsI GCAGTG_nn'
Cuts at: 2210 2210
Size: 4967
DraI TTT'AAA
Cuts at: 1556 1966 1556
Size: 410 4557
DrdI GACnn_nn'nnGTC
Cuts at: 2673 4017 2673
Size: 1344 3623
EaeI y'GGCC_r
Cuts at: 688 943 1667 2552 2726 3117 3144 4489 688
Size: 255 724 885 174 391 27 1345 1166
Fragments arranged by size:
1345 1166 885 724 391 255 174 27
EagI C'GGCC_G
Cuts at: 2552 2552
Size: 4967
EarI CTCTTCn'nnn_
Cuts at: 621 1430 2990 3200 621
Size: 809 1560 210 2388
Fragments arranged by size:
2388 1560 809 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3175 3917 4063 4777 3175
Size: 742 146 714 3365
Fragments arranged by size:
3365 742 714 146
EcoICRI GAG'CTC
Cuts at: 1979 1979
Size: 4967
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 677 770 1082 1178 1226 1244 1268 1388
Size: 125 93 312 96 48 18 24 120
Cuts at: 1388 1439 1478 2792 3224 3577 4239 552
Size: 51 39 1314 432 353 662 1280
Fragments arranged by size:
1314 1280 662 432 353 312 125 120
96 93 51 48 39 24 18
EcoO109I rG'GnC_Cy
Cuts at: 142 745 2106 142
Size: 603 1361 3003
Fragments arranged by size:
3003 1361 603
EcoRI G'AATT_C
Cuts at: 1971 1971
Size: 4967
EcoRV GAT'ATC
Cuts at: 1162 1162
Size: 4967
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 249 2986 249
Size: 2737 2230
FspI TGC'GCA
Cuts at: 2748 2748
Size: 4967
HaeII r_GCGC'y
Cuts at: 2401 2649 3879 4650 2401
Size: 248 1230 771 2718
Fragments arranged by size:
2718 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 802 834 1384 1416 1465 1497 2809 2839 2841
Size: 32 550 32 49 32 1312 30 2
Cuts at: 2841 2871 802
Size: 30 2898
Fragments arranged by size:
2898 1312 550 49 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1190 2006 1190
Size: 816 4151
KasI G'GCGC_C
Cuts at: 2397 2645 2397
Size: 248 4719
KpnI G_GTAC'C
Cuts at: 1987 1987
Size: 4967
MfeI C'AATT_G
Cuts at: 1519 1789 1519
Size: 270 4697
MluI A'CGCG_T
Cuts at: 4400 4400
Size: 4967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1777 3726 3910 1777
Size: 1949 184 2834
Fragments arranged by size:
2834 1949 184
MscI TGG'CCA
Cuts at: 690 945 2728 690
Size: 255 1783 2929
Fragments arranged by size:
2929 1783 255
MslI CAynn'nnrTG
Cuts at: 2956 3083 2956
Size: 127 4840
MspA1I CmG'CkG
Cuts at: 1182 2078 2392 2752 3536 3781 1182
Size: 896 314 360 784 245 2368
Fragments arranged by size:
2368 896 784 360 314 245
NaeI GCC'GGC
Cuts at: 3148 3148
Size: 4967
NarI GG'CG_CC
Cuts at: 2398 2646 2398
Size: 248 4719
NcoI C'CATG_G
Cuts at: 1072 1072
Size: 4967
NdeI CA'TA_TG
Cuts at: 683 683
Size: 4967
NgoMIV G'CCGG_C
Cuts at: 3146 3146
Size: 4967
NspI r_CATG'y
Cuts at: 906 916 1087 1529 2020 2520 4123 906
Size: 10 171 442 491 500 1603 1750
Fragments arranged by size:
1750 1603 500 491 442 171 10
PciI A'CATG_T
Cuts at: 1525 4119 1525
Size: 2594 2373
PfoI T'CCnGG_A
Cuts at: 1541 1541
Size: 4967
PmeI GTTT'AAAC
Cuts at: 1966 1966
Size: 4967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 4935
PpuMI rG'GwC_Cy
Cuts at: 142 745 142
Size: 603 4364
PstI C_TGCA'G
Cuts at: 2014 2699 2014
Size: 685 4282
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4967
PvuII CAG'CTG
Cuts at: 1182 2392 2752 1182
Size: 1210 360 3397
Fragments arranged by size:
3397 1210 360
RsrII CG'GwC_CG
Cuts at: 3162 3162
Size: 4967
SacI G_AGCT'C
Cuts at: 1981 1981
Size: 4967
SalI G'TCGA_C
Cuts at: 2004 2004
Size: 4967
SapI GCTCTTCn'nnn_
Cuts at: 2990 3200 2990
Size: 210 4757
SbfI CC_TGCA'GG
Cuts at: 2014 2014
Size: 4967
ScaI AGT'ACT
Cuts at: 654 4484 654
Size: 3830 1137
SexAI A'CCwGG_T
Cuts at: 1328 1328
Size: 4967
SfcI C'TryA_G
Cuts at: 33 249 435 636 2010 2695 3663 3854 33
Size: 216 186 201 1374 685 968 191 1146
Fragments arranged by size:
1374 1146 968 685 216 201 191 186
SfoI GGC'GCC
Cuts at: 2399 2647 2399
Size: 248 4719
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4967
SmaI CCC'GGG
Cuts at: 1989 1989
Size: 4967
SmlI C'TyrA_G
Cuts at: 1044 2122 3037 3751 4013 1044
Size: 1078 915 714 262 1998
Fragments arranged by size:
1998 1078 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4967
SphI G_CATG'C
Cuts at: 906 2020 2520 906
Size: 1114 500 3353
Fragments arranged by size:
3353 1114 500
StyI C'CwwG_G
Cuts at: 533 1072 1099 1304 2101 2455 533
Size: 539 27 205 797 354 3045
Fragments arranged by size:
3045 797 539 354 205 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2550 2683 3142 3351 2550
Size: 133 459 209 4166
Fragments arranged by size:
4166 459 209 133
TatI w'GTAC_w
Cuts at: 652 938 4482 652
Size: 286 3544 1137
Fragments arranged by size:
3544 1137 286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4484 4516 4484
Size: 32 4935
Tth111I GACn'n_nGTC
Cuts at: 346 2764 346
Size: 2418 2549
XbaI T'CTAG_A
Cuts at: 595 867 1998 595
Size: 272 1131 3564
Fragments arranged by size:
3564 1131 272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2462 2462
Size: 4967
XmaI C'CCGG_G
Cuts at: 1987 1987
Size: 4967
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI