Restriction map for
pF1KB3064
(Circular) (Six-base) MAPSORT of: pF1KB3064.seq Check: 4910 from: 1 to: 4343
pF1KB3064 4343 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1381 1381
Size: 4343
Acc65I G'GTAC_C
Cuts at: 1359 1359
Size: 4343
AclI AA'CG_TT
Cuts at: 4183 4183
Size: 4343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 680 1010 2168 2600 2953 3615 470
Size: 210 330 1158 432 353 662 1198
Fragments arranged by size:
1198 1158 662 432 353 330 210
AflIII A'CryG_T
Cuts at: 1194 1695 3495 3776 1194
Size: 501 1800 281 1761
Fragments arranged by size:
1800 1761 501 281
AlwNI CAG_nnn'CTG
Cuts at: 562 1003 1648 3086 562
Size: 441 645 1438 1819
Fragments arranged by size:
1819 1438 645 441
ApaLI G'TGCA_C
Cuts at: 3181 3181
Size: 4343
ApoI r'AATT_y
Cuts at: 340 1347 3696 3952 340
Size: 1007 2349 256 731
Fragments arranged by size:
2349 1007 731 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4343
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
AvaI C'yCGr_G
Cuts at: 443 1363 443
Size: 920 3423
BamHI G'GATC_C
Cuts at: 1048 1368 1048
Size: 320 4023
BanI G'GyrC_C
Cuts at: 1281 1359 1773 2021 2056 1281
Size: 78 414 248 35 3568
Fragments arranged by size:
3568 414 248 78 35
BanII G_rGCy'C
Cuts at: 923 963 1357 2387 923
Size: 40 394 1030 2879
Fragments arranged by size:
2879 1030 394 40
BbeI G_GCGC'C
Cuts at: 1777 2025 1777
Size: 248 4095
BbsI GAAGACnn'nnnn_
Cuts at: 796 1234 796
Size: 438 3905
BciVI GTATCCnnnnn_n'
Cuts at: 2241 3297 4343 2241
Size: 1056 1046 2241
Fragments arranged by size:
2241 1056 1046
BclI T'GATC_A
Cuts at: 1863 1863
Size: 4343
BfrBI ATG'CAT
Cuts at: 415 659 950 1223 415
Size: 244 291 273 3535
Fragments arranged by size:
3535 291 273 244
BglII A'GATC_T
Cuts at: 1858 3895 1858
Size: 2037 2306
BlpI GC'TnA_GC
Cuts at: 917 1455 917
Size: 538 3805
Bme1580I G_kGCm'C
Cuts at: 1968 2061 3185 1968
Size: 93 1124 3126
Fragments arranged by size:
3126 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1134 1555 1636 1971 4115 1134
Size: 421 81 335 2144 1362
Fragments arranged by size:
2144 1362 421 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 4343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 947 545
Size: 402 3941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 1519 2398 3112 3410 723
Size: 796 879 714 298 1656
Fragments arranged by size:
1656 879 796 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 368 483 4047 368
Size: 115 3564 664
Fragments arranged by size:
3564 664 115
BsaAI yAC'GTr
Cuts at: 820 1132 1696 820
Size: 312 564 3467
Fragments arranged by size:
3467 564 312
BsaBI GATnn'nnATC
Cuts at: 6 1857 3894 6
Size: 1851 2037 455
Fragments arranged by size:
2037 1851 455
BsaHI Gr'CG_yC
Cuts at: 1774 2022 2724 4220 1774
Size: 248 702 1496 1897
Fragments arranged by size:
1897 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1532 2053 2731 3142 3289 3563 1532
Size: 521 678 411 147 274 2312
Fragments arranged by size:
2312 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1768 2526 3191 3599 1768
Size: 758 665 408 2512
Fragments arranged by size:
2512 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1885 1931 3161 104
Size: 1781 46 1230 1286
Fragments arranged by size:
1781 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 923 1357 2135 2325 3185 923
Size: 434 778 190 860 2081
Fragments arranged by size:
2081 860 778 434 190
BsmI GAATG_Cn'
Cuts at: 661 913 952 661
Size: 252 39 4052
Fragments arranged by size:
4052 252 39
Bsp1286I G_dGCh'C
Cuts at: 923 963 1357 1968 2061 2135 2325 2387 3185
Size: 40 394 611 93 74 190 62 798
Cuts at: 3185 923
Size: 2081
Fragments arranged by size:
2081 798 611 394 190 93 74 62
40
BspHI T'CATG_A
Cuts at: 1116 2775 1116
Size: 1659 2684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1393 1607 1909 2290 1393
Size: 214 302 381 3446
Fragments arranged by size:
3446 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2635 2689 4173 4338 50
Size: 2585 54 1484 165 55
Fragments arranged by size:
2585 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2255 2255
Size: 4343
BsrFI r'CCGG_y
Cuts at: 2341 2522 2341
Size: 181 4162
BsrGI T'GTAC_A
Cuts at: 564 564
Size: 4343
BssSI C'ACGA_G
Cuts at: 1108 2614 3322 1108
Size: 1506 708 2129
Fragments arranged by size:
2129 1506 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1720 1720
Size: 4343
BstBI TT'CG_AA
Cuts at: 2704 2704
Size: 4343
BstXI CCAn_nnnn'nTGG
Cuts at: 386 386
Size: 4343
BstYI r'GATC_y
Cuts at: 1 1000 1048 1368 1835 1858 2193 2439 2745
Size: 999 48 320 467 23 335 246 306
Cuts at: 2745 2757 2843 3895 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 999 467 449 335 320 306 246
86 48 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1233 2293 2474 2637 1233
Size: 1060 181 163 2939
Fragments arranged by size:
2939 1060 181 163
BtsI GCAGTG_nn'
Cuts at: 1586 1586
Size: 4343
DraI TTT'AAA
Cuts at: 201 1342 201
Size: 1141 3202
DrdI GACnn_nn'nnGTC
Cuts at: 362 2049 3393 362
Size: 1687 1344 1312
Fragments arranged by size:
1687 1344 1312
EaeI y'GGCC_r
Cuts at: 675 829 1928 2102 2493 2520 3865 675
Size: 154 1099 174 391 27 1345 1153
Fragments arranged by size:
1345 1153 1099 391 174 154 27
EagI C'GGCC_G
Cuts at: 1928 1928
Size: 4343
EarI CTCTTCn'nnn_
Cuts at: 176 2366 2576 176
Size: 2190 210 1943
Fragments arranged by size:
2190 1943 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 389 2551 3293 3439 4153 389
Size: 2162 742 146 714 579
Fragments arranged by size:
2162 742 714 579 146
EcoICRI GAG'CTC
Cuts at: 921 1355 921
Size: 434 3909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 545 680 947 1010 2168 2600 2953 3615
Size: 75 135 267 63 1158 432 353 662
Cuts at: 3615 470
Size: 1198
Fragments arranged by size:
1198 1158 662 432 353 267 135 75
63
EcoO109I rG'GnC_Cy
Cuts at: 1482 1482
Size: 4343
EcoRI G'AATT_C
Cuts at: 1347 1347
Size: 4343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 4343
FspI TGC'GCA
Cuts at: 1241 2124 1241
Size: 883 3460
FspAI rTGC'GCAy
Cuts at: 1241 1241
Size: 4343
HaeII r_GCGC'y
Cuts at: 1777 2025 3255 4026 1777
Size: 248 1230 771 2094
Fragments arranged by size:
2094 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 381 413 2185 2215 2217 2247 381
Size: 32 1772 30 2 30 2477
Fragments arranged by size:
2477 1772 32 30 30 2
HincII GTy'rAC
Cuts at: 1382 1382
Size: 4343
HindIII A'AGCT_T
Cuts at: 595 595
Size: 4343
KasI G'GCGC_C
Cuts at: 1773 2021 1773
Size: 248 4095
KpnI G_GTAC'C
Cuts at: 1363 1363
Size: 4343
MluI A'CGCG_T
Cuts at: 1194 3776 1194
Size: 2582 1761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3102 3286 3102
Size: 184 4159
MscI TGG'CCA
Cuts at: 2104 2104
Size: 4343
MslI CAynn'nnrTG
Cuts at: 677 935 2332 2459 677
Size: 258 1397 127 2561
Fragments arranged by size:
2561 1397 258 127
MspA1I CmG'CkG
Cuts at: 590 860 1454 1768 2128 2912 3157 590
Size: 270 594 314 360 784 245 1776
Fragments arranged by size:
1776 784 594 360 314 270 245
NaeI GCC'GGC
Cuts at: 2524 2524
Size: 4343
NarI GG'CG_CC
Cuts at: 1774 2022 1774
Size: 248 4095
NcoI C'CATG_G
Cuts at: 107 107
Size: 4343
NgoMIV G'CCGG_C
Cuts at: 2522 2522
Size: 4343
NsiI A_TGCA'T
Cuts at: 417 661 952 1225 417
Size: 244 291 273 3535
Fragments arranged by size:
3535 291 273 244
NspI r_CATG'y
Cuts at: 940 1095 1396 1896 3499 940
Size: 155 301 500 1603 1784
Fragments arranged by size:
1784 1603 500 301 155
PciI A'CATG_T
Cuts at: 3495 3495
Size: 4343
PmeI GTTT'AAAC
Cuts at: 1342 1342
Size: 4343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 4311
PspXI vC'TCGA_Gb
Cuts at: 443 443
Size: 4343
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1121 1153 1121
Size: 32 4311
PstI C_TGCA'G
Cuts at: 618 1390 2075 618
Size: 772 685 2886
Fragments arranged by size:
2886 772 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4343
PvuII CAG'CTG
Cuts at: 860 1768 2128 860
Size: 908 360 3075
Fragments arranged by size:
3075 908 360
RsrII CG'GwC_CG
Cuts at: 2538 2538
Size: 4343
SacI G_AGCT'C
Cuts at: 923 1357 923
Size: 434 3909
SalI G'TCGA_C
Cuts at: 1380 1380
Size: 4343
SapI GCTCTTCn'nnn_
Cuts at: 2366 2576 2366
Size: 210 4133
SbfI CC_TGCA'GG
Cuts at: 1390 1390
Size: 4343
ScaI AGT'ACT
Cuts at: 1086 3860 1086
Size: 2774 1569
SfcI C'TryA_G
Cuts at: 33 149 614 1386 2071 3039 3230 33
Size: 116 465 772 685 968 191 1146
Fragments arranged by size:
1146 968 772 685 465 191 116
SfoI GGC'GCC
Cuts at: 1775 2023 1775
Size: 248 4095
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
SmaI CCC'GGG
Cuts at: 1365 1365
Size: 4343
SmlI C'TyrA_G
Cuts at: 443 738 1498 2413 3127 3389 443
Size: 295 760 915 714 262 1397
Fragments arranged by size:
1397 915 760 714 295 262
SnaBI TAC'GTA
Cuts at: 820 820
Size: 4343
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4343
SphI G_CATG'C
Cuts at: 1396 1896 1396
Size: 500 3843
StyI C'CwwG_G
Cuts at: 107 1262 1477 1831 107
Size: 1155 215 354 2619
Fragments arranged by size:
2619 1155 354 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1926 2059 2518 2727 1926
Size: 133 459 209 3542
Fragments arranged by size:
3542 459 209 133
TatI w'GTAC_w
Cuts at: 564 1084 3858 564
Size: 520 2774 1049
Fragments arranged by size:
2774 1049 520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3860 3892 3860
Size: 32 4311
Tth111I GACn'n_nGTC
Cuts at: 2140 2140
Size: 4343
XbaI T'CTAG_A
Cuts at: 1374 1374
Size: 4343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1838 1838
Size: 4343
XhoI C'TCGA_G
Cuts at: 443 443
Size: 4343
XmaI C'CCGG_G
Cuts at: 1363 1363
Size: 4343
XmnI GAAnn'nnTTC
Cuts at: 389 793 389
Size: 404 3939
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI ZraI