Restriction map for
pF1KB3061
(Circular) (Six-base) MAPSORT of: pF1KB3061.seq Check: 6293 from: 1 to: 4778
pF1KB3061 4778 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1816 1816
Size: 4778
Acc65I G'GTAC_C
Cuts at: 1794 1794
Size: 4778
AclI AA'CG_TT
Cuts at: 4618 4618
Size: 4778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 783 1119 1203 1287 1455 1539 1623 2603
Size: 62 336 84 84 168 84 84 980
Cuts at: 2603 3035 3388 4050 721
Size: 432 353 662 1449
Fragments arranged by size:
1449 980 662 432 353 336 168 84
84 84 84 62
AflII C'TTAA_G
Cuts at: 630 630
Size: 4778
AflIII A'CryG_T
Cuts at: 689 1193 2130 3930 4211 689
Size: 504 937 1800 281 1256
Fragments arranged by size:
1800 1256 937 504 281
AleI CACnn'nnGTG
Cuts at: 842 1514 842
Size: 672 4106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1084 1084
Size: 4778
AlwNI CAG_nnn'CTG
Cuts at: 372 2083 3521 372
Size: 1711 1438 1629
Fragments arranged by size:
1711 1629 1438
ApaLI G'TGCA_C
Cuts at: 1255 1475 1533 1579 1759 3616 1255
Size: 220 58 46 180 1857 2417
Fragments arranged by size:
2417 1857 220 180 58 46
ApoI r'AATT_y
Cuts at: 833 1782 4131 4387 833
Size: 949 2349 256 1224
Fragments arranged by size:
2349 1224 949 256
AseI AT'TA_AT
Cuts at: 20 505 20
Size: 485 4293
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
AvaI C'yCGr_G
Cuts at: 1798 1798
Size: 4778
BamHI G'GATC_C
Cuts at: 286 1001 1169 1337 1673 1803 286
Size: 715 168 168 336 130 3261
Fragments arranged by size:
3261 715 336 168 168 130
BanI G'GyrC_C
Cuts at: 1794 2208 2456 2491 1794
Size: 414 248 35 4081
Fragments arranged by size:
4081 414 248 35
BanII G_rGCy'C
Cuts at: 811 895 1792 2822 811
Size: 84 897 1030 2767
Fragments arranged by size:
2767 1030 897 84
BbeI G_GCGC'C
Cuts at: 2212 2460 2212
Size: 248 4530
BbsI GAAGACnn'nnnn_
Cuts at: 1303 1303
Size: 4778
BciVI GTATCCnnnnn_n'
Cuts at: 760 2676 3732 4778 760
Size: 1916 1056 1046 760
Fragments arranged by size:
1916 1056 1046 760
BclI T'GATC_A
Cuts at: 555 2298 555
Size: 1743 3035
BfrBI ATG'CAT
Cuts at: 1363 1363
Size: 4778
BglII A'GATC_T
Cuts at: 897 2293 4330 897
Size: 1396 2037 1345
Fragments arranged by size:
2037 1396 1345
BlpI GC'TnA_GC
Cuts at: 905 1890 905
Size: 985 3793
Bme1580I G_kGCm'C
Cuts at: 214 1259 1466 1479 1537 1583 1763 2403 2496
Size: 1045 207 13 58 46 180 640 93
Cuts at: 2496 3620 214
Size: 1124 1372
Fragments arranged by size:
1372 1124 1045 640 207 180 93 58
46 13
BmrI ACTGGGnnnn_n'
Cuts at: 1103 1439 1990 2071 2406 4550 1103
Size: 336 551 81 335 2144 1331
Fragments arranged by size:
2144 1331 551 336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3145 3145
Size: 4778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 779 334
Size: 445 4333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 510 970 1954 2833 3547 3845 510
Size: 460 984 879 714 298 1443
Fragments arranged by size:
1443 984 879 714 460 298
BsaI GGTCTCn'nnnn_
Cuts at: 1490 4482 1490
Size: 2992 1786
BsaAI yAC'GTr
Cuts at: 1196 2131 1196
Size: 935 3843
BsaBI GATnn'nnATC
Cuts at: 6 2292 4329 6
Size: 2286 2037 455
Fragments arranged by size:
2286 2037 455
BsaHI Gr'CG_yC
Cuts at: 2209 2457 3159 4655 2209
Size: 248 702 1496 2332
Fragments arranged by size:
2332 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1967 2488 3166 3577 3724 3998 1967
Size: 521 678 411 147 274 2747
Fragments arranged by size:
2747 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 289 319 289
Size: 30 4748
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 172
Size: 4778
BseYI C'CCAG_C
Cuts at: 308 2203 2961 3626 4034 308
Size: 1895 758 665 408 1052
Fragments arranged by size:
1895 1052 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 798 1218 1302 798
Size: 420 84 4274
Fragments arranged by size:
4274 420 84
BsiEI CG_ry'CG
Cuts at: 104 1227 2320 2366 3596 104
Size: 1123 1093 46 1230 1286
Fragments arranged by size:
1286 1230 1123 1093 46
BsiHKAI G_wGCw'C
Cuts at: 182 811 895 1259 1479 1537 1583 1658 1763
Size: 629 84 364 220 58 46 75 105
Cuts at: 1763 1792 2570 2760 3620 182
Size: 29 778 190 860 1340
Fragments arranged by size:
1340 860 778 629 364 220 190 105
84 75 58 46 29
Bsp1286I G_dGCh'C
Cuts at: 182 214 811 895 1259 1466 1479 1537 1583
Size: 32 597 84 364 207 13 58 46
Cuts at: 1583 1658 1763 1792 2403 2496 2570 2760 2822
Size: 75 105 29 611 93 74 190 62
Cuts at: 2822 3620 182
Size: 798 1340
Fragments arranged by size:
1340 798 611 597 364 207 190 105
93 84 75 74 62 58 46 32
29 13
BspHI T'CATG_A
Cuts at: 3210 3210
Size: 4778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1828 2042 2344 2725 1828
Size: 214 302 381 3881
Fragments arranged by size:
3881 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1229 1313 1335 3070 3124 4608 4773 50
Size: 1179 84 22 1735 54 1484 165 55
Fragments arranged by size:
1735 1484 1179 165 84 55 54 22
BsrDI GCAATG_nn'
Cuts at: 1122 2690 1122
Size: 1568 3210
BsrFI r'CCGG_y
Cuts at: 135 1481 2776 2957 135
Size: 1346 1295 181 1956
Fragments arranged by size:
1956 1346 1295 181
BsrGI T'GTAC_A
Cuts at: 421 421
Size: 4778
BssSI C'ACGA_G
Cuts at: 1330 3049 3757 1330
Size: 1719 708 2351
Fragments arranged by size:
2351 1719 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1625 2155 1625
Size: 530 4248
BstBI TT'CG_AA
Cuts at: 3139 3139
Size: 4778
BstYI r'GATC_y
Cuts at: 1 286 327 897 1001 1169 1337 1673 1803
Size: 285 41 570 104 168 168 336 130
Cuts at: 1803 2270 2293 2628 2874 3180 3192 3278 4330
Size: 467 23 335 246 306 12 86 1052
Cuts at: 4330 1
Size: 449
Fragments arranged by size:
1052 570 467 449 336 335 306 285
246 168 168 130 104 86 41 23
12
Bsu36I CC'TnA_GG
Cuts at: 1585 1585
Size: 4778
BtgI C'CryG_G
Cuts at: 107 448 107
Size: 341 4437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2728 2909 3072 2728
Size: 181 163 4434
Fragments arranged by size:
4434 181 163
BtsI GCAGTG_nn'
Cuts at: 671 786 1150 1290 2021 671
Size: 115 364 140 731 3428
Fragments arranged by size:
3428 731 364 140 115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1104 1139 1104
Size: 35 4743
DraI TTT'AAA
Cuts at: 1777 1777
Size: 4778
DraIII CAC_nnn'GTG
Cuts at: 1254 1254
Size: 4778
DrdI GACnn_nn'nnGTC
Cuts at: 1231 2484 3828 1231
Size: 1253 1344 2181
Fragments arranged by size:
2181 1344 1253
EaeI y'GGCC_r
Cuts at: 717 1734 2363 2537 2928 2955 4300 717
Size: 1017 629 174 391 27 1345 1195
Fragments arranged by size:
1345 1195 1017 629 391 174 27
EagI C'GGCC_G
Cuts at: 2363 2363
Size: 4778
EarI CTCTTCn'nnn_
Cuts at: 355 1138 2801 3011 355
Size: 783 1663 210 2122
Fragments arranged by size:
2122 1663 783 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2986 3728 3874 4588 2986
Size: 742 146 714 3176
Fragments arranged by size:
3176 742 714 146
EcoICRI GAG'CTC
Cuts at: 809 893 1790 809
Size: 84 897 3797
Fragments arranged by size:
3797 897 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 721 779 783 1119 1203 1287 1455 1539
Size: 387 58 4 336 84 84 168 84
Cuts at: 1539 1623 2603 3035 3388 4050 334
Size: 84 980 432 353 662 1062
Fragments arranged by size:
1062 980 662 432 387 353 336 168
84 84 84 84 58 4
EcoNI CCTnn'n_nnAGG
Cuts at: 187 546 187
Size: 359 4419
EcoO109I rG'GnC_Cy
Cuts at: 142 225 336 369 683 1589 1917 142
Size: 83 111 33 314 906 328 3003
Fragments arranged by size:
3003 906 328 314 111 83 33
EcoRI G'AATT_C
Cuts at: 833 1782 833
Size: 949 3829
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 177 600 2797 177
Size: 423 2197 2158
Fragments arranged by size:
2197 2158 423
FspI TGC'GCA
Cuts at: 2559 2559
Size: 4778
HaeII r_GCGC'y
Cuts at: 2212 2460 3690 4461 2212
Size: 248 1230 771 2529
Fragments arranged by size:
2529 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 424 456 540 572 882 914 2620
Size: 32 103 32 84 32 310 32 1706
Cuts at: 2620 2650 2652 2682 289
Size: 30 2 30 2385
Fragments arranged by size:
2385 1706 310 103 84 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 609 1817 609
Size: 1208 3570
HindIII A'AGCT_T
Cuts at: 582 582
Size: 4778
KasI G'GCGC_C
Cuts at: 2208 2456 2208
Size: 248 4530
KpnI G_GTAC'C
Cuts at: 1798 1798
Size: 4778
MluI A'CGCG_T
Cuts at: 4211 4211
Size: 4778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 937 3537 3721 937
Size: 2600 184 1994
Fragments arranged by size:
2600 1994 184
MscI TGG'CCA
Cuts at: 1736 2539 1736
Size: 803 3975
MslI CAynn'nnrTG
Cuts at: 453 842 1026 1321 1514 1614 2767 2894 453
Size: 389 184 295 193 100 1153 127 2337
Fragments arranged by size:
2337 1153 389 295 193 184 127 100
MspA1I CmG'CkG
Cuts at: 312 999 1671 1889 2203 2563 3347 3592 312
Size: 687 672 218 314 360 784 245 1498
Fragments arranged by size:
1498 784 687 672 360 314 245 218
NaeI GCC'GGC
Cuts at: 137 2959 137
Size: 2822 1956
NarI GG'CG_CC
Cuts at: 2209 2457 2209
Size: 248 4530
NcoI C'CATG_G
Cuts at: 107 448 107
Size: 341 4437
NdeI CA'TA_TG
Cuts at: 1023 1611 1023
Size: 588 4190
NgoMIV G'CCGG_C
Cuts at: 135 2957 135
Size: 2822 1956
NsiI A_TGCA'T
Cuts at: 1365 1365
Size: 4778
NspI r_CATG'y
Cuts at: 645 693 1367 1831 2331 3934 645
Size: 48 674 464 500 1603 1489
Fragments arranged by size:
1603 1489 674 500 464 48
PasI CC'CwG_GG
Cuts at: 332 332
Size: 4778
PciI A'CATG_T
Cuts at: 689 3930 689
Size: 3241 1537
PfoI T'CCnGG_A
Cuts at: 282 338 282
Size: 56 4722
PmeI GTTT'AAAC
Cuts at: 1777 1777
Size: 4778
PmlI CAC'GTG
Cuts at: 1196 1196
Size: 4778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 4746
PpuMI rG'GwC_Cy
Cuts at: 142 336 1589 142
Size: 194 1253 3331
Fragments arranged by size:
3331 1253 194
PshAI GACnn'nnGTC
Cuts at: 491 491
Size: 4778
PstI C_TGCA'G
Cuts at: 1160 1825 2510 1160
Size: 665 685 3428
Fragments arranged by size:
3428 685 665
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4778
PvuII CAG'CTG
Cuts at: 312 2203 2563 312
Size: 1891 360 2527
Fragments arranged by size:
2527 1891 360
RsrII CG'GwC_CG
Cuts at: 2973 2973
Size: 4778
SacI G_AGCT'C
Cuts at: 811 895 1792 811
Size: 84 897 3797
Fragments arranged by size:
3797 897 84
SalI G'TCGA_C
Cuts at: 1815 1815
Size: 4778
SapI GCTCTTCn'nnn_
Cuts at: 355 2801 3011 355
Size: 2446 210 2122
Fragments arranged by size:
2446 2122 210
SbfI CC_TGCA'GG
Cuts at: 1825 1825
Size: 4778
ScaI AGT'ACT
Cuts at: 1648 4295 1648
Size: 2647 2131
SfcI C'TryA_G
Cuts at: 33 231 413 1156 1821 2506 3474 3665 33
Size: 198 182 743 665 685 968 191 1146
Fragments arranged by size:
1146 968 743 685 665 198 191 182
SfoI GGC'GCC
Cuts at: 2210 2458 2210
Size: 248 4530
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
SmaI CCC'GGG
Cuts at: 1800 1800
Size: 4778
SmlI C'TyrA_G
Cuts at: 489 630 949 1933 2848 3562 3824 489
Size: 141 319 984 915 714 262 1443
Fragments arranged by size:
1443 984 915 714 319 262 141
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4778
SphI G_CATG'C
Cuts at: 645 1367 1831 2331 645
Size: 722 464 500 3092
Fragments arranged by size:
3092 722 500 464
StuI AGG'CCT
Cuts at: 796 1216 1384 1636 796
Size: 420 168 252 3938
Fragments arranged by size:
3938 420 252 168
StyI C'CwwG_G
Cuts at: 107 448 464 542 1912 2266 107
Size: 341 16 78 1370 354 2619
Fragments arranged by size:
2619 1370 354 341 78 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2361 2494 2953 3162 2361
Size: 133 459 209 3977
Fragments arranged by size:
3977 459 209 133
TatI w'GTAC_w
Cuts at: 421 1646 4293 421
Size: 1225 2647 906
Fragments arranged by size:
2647 1225 906
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 193 225 1391 1423 4295 4327 193
Size: 32 1166 32 2872 32 644
Fragments arranged by size:
2872 1166 644 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2575 2575
Size: 4778
XbaI T'CTAG_A
Cuts at: 1809 1809
Size: 4778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2273 2273
Size: 4778
XmaI C'CCGG_G
Cuts at: 1798 1798
Size: 4778
XmnI GAAnn'nnTTC
Cuts at: 796 964 1048 1132 1216 1300 1384 1636 796
Size: 168 84 84 84 84 84 252 3938
Fragments arranged by size:
3938 252 168 84 84 84 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BspEI BssHII BstEII BstXI BstZ17I
ClaI EcoRV FseI FspAI HpaI MfeI NheI NotI
NruI PacI PflMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI