Restriction map for
pF1KB3050
(Circular) (Six-base) MAPSORT of: pF1KB3050.seq Check: 976 from: 1 to: 4733
pF1KB3050 4733 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 424 424
Size: 4733
AccI GT'mk_AC
Cuts at: 1771 1771
Size: 4733
Acc65I G'GTAC_C
Cuts at: 1749 1749
Size: 4733
AclI AA'CG_TT
Cuts at: 4573 4573
Size: 4733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 702 2558 2990 3737 4005 702
Size: 1856 432 747 268 1430
Fragments arranged by size:
1856 1430 747 432 268
AflIII A'CryG_T
Cuts at: 280 1442 2085 3189 4166 280
Size: 1162 643 1104 977 847
Fragments arranged by size:
1162 1104 977 847 643
AgeI A'CCGG_T
Cuts at: 3121 3121
Size: 4733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 821 821
Size: 4733
AlwNI CAG_nnn'CTG
Cuts at: 1020 1033 2038 3605 1020
Size: 13 1005 1567 2148
Fragments arranged by size:
2148 1567 1005 13
ApaI G_GGCC'C
Cuts at: 169 1428 169
Size: 1259 3474
ApaLI G'TGCA_C
Cuts at: 3503 3503
Size: 4733
ApoI r'AATT_y
Cuts at: 511 1737 4086 4342 511
Size: 1226 2349 256 902
Fragments arranged by size:
2349 1226 902 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4733
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
AvaI C'yCGr_G
Cuts at: 301 567 1541 1753 301
Size: 266 974 212 3281
Fragments arranged by size:
3281 974 266 212
BamHI G'GATC_C
Cuts at: 273 1758 273
Size: 1485 3248
BanI G'GyrC_C
Cuts at: 1059 1181 1282 1608 1749 2163 2411 2446 1059
Size: 122 101 326 141 414 248 35 3346
Fragments arranged by size:
3346 414 326 248 141 122 101 35
BanII G_rGCy'C
Cuts at: 169 1325 1428 1513 1747 2777 169
Size: 1156 103 85 234 1030 2125
Fragments arranged by size:
2125 1156 1030 234 103 85
BbeI G_GCGC'C
Cuts at: 2167 2415 2167
Size: 248 4485
BbsI GAAGACnn'nnnn_
Cuts at: 1051 1235 1051
Size: 184 4549
BbvCI CC'TCA_GC
Cuts at: 227 227
Size: 4733
BciVI GTATCCnnnnn_n'
Cuts at: 2631 3392 4733 2631
Size: 761 1341 2631
Fragments arranged by size:
2631 1341 761
BclI T'GATC_A
Cuts at: 2253 2253
Size: 4733
BglI GCCn_nnn'nGGC
Cuts at: 238 829 1024 1300 238
Size: 591 195 276 3671
Fragments arranged by size:
3671 591 276 195
BglII A'GATC_T
Cuts at: 2248 4285 2248
Size: 2037 2696
BlpI GC'TnA_GC
Cuts at: 790 1845 790
Size: 1055 3678
Bme1580I G_kGCm'C
Cuts at: 169 1186 1428 1613 1631 2358 2451 3507 169
Size: 1017 242 185 18 727 93 1056 1395
Fragments arranged by size:
1395 1056 1017 727 242 185 93 18
BmrI ACTGGGnnnn_n'
Cuts at: 546 637 1264 1945 2026 2361 4505 546
Size: 91 627 681 81 335 2144 774
Fragments arranged by size:
2144 774 681 627 335 91 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 906 1532 3100 906
Size: 626 1568 2539
Fragments arranged by size:
2539 1568 626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 898 1142 1735 856
Size: 42 244 593 3854
Fragments arranged by size:
3854 593 244 42
Bpu10I CC'TnA_GC
Cuts at: 227 956 227
Size: 729 4004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 1078 1909 2788 3280 3578 220
Size: 858 831 879 492 298 1375
Fragments arranged by size:
1375 879 858 831 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4437 4437
Size: 4733
BsaAI yAC'GTr
Cuts at: 2086 2086
Size: 4733
BsaBI GATnn'nnATC
Cuts at: 6 2247 4284 6
Size: 2241 2037 455
Fragments arranged by size:
2241 2037 455
BsaHI Gr'CG_yC
Cuts at: 2164 2412 3114 4610 2164
Size: 248 702 1496 2287
Fragments arranged by size:
2287 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1922 2443 3121 3395 3542 3953 1922
Size: 521 678 274 147 411 2702
Fragments arranged by size:
2702 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 871 901 1678 1708 871
Size: 30 777 30 3896
Fragments arranged by size:
3896 777 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 853 895 1069 217
Size: 636 42 174 3881
Fragments arranged by size:
3881 636 174 42
BseYI C'CCAG_C
Cuts at: 428 687 1498 2158 2916 3493 3989 428
Size: 259 811 660 758 577 496 1172
Fragments arranged by size:
1172 811 758 660 577 496 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 688 315
Size: 373 4360
BsiEI CG_ry'CG
Cuts at: 104 2275 2321 3529 104
Size: 2171 46 1208 1308
Fragments arranged by size:
2171 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1128 1478 1747 2525 2715 3507 1128
Size: 350 269 778 190 792 2354
Fragments arranged by size:
2354 792 778 350 269 190
Bsp1286I G_dGCh'C
Cuts at: 169 1128 1186 1325 1428 1478 1513 1613 1631
Size: 959 58 139 103 50 35 100 18
Cuts at: 1631 1747 2358 2451 2525 2715 2777 3507 169
Size: 116 611 93 74 190 62 730 1395
Fragments arranged by size:
1395 959 730 611 190 139 116 103
100 93 74 62 58 50 35 18
BspEI T'CCGG_A
Cuts at: 3953 3953
Size: 4733
BspHI T'CATG_A
Cuts at: 3909 3909
Size: 4733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 424 1718 1783 1997 2299 2680 424
Size: 1294 65 214 302 381 2477
Fragments arranged by size:
2477 1294 381 302 214 65
BsrBI CCG'CTC
Cuts at: 50 218 546 3025 3079 4563 4728 50
Size: 168 328 2479 54 1484 165 55
Fragments arranged by size:
2479 1484 328 168 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1535 2645 1535
Size: 1110 3623
BsrFI r'CCGG_y
Cuts at: 185 2731 2912 3121 185
Size: 2546 181 209 1797
Fragments arranged by size:
2546 1797 209 181
BssSI C'ACGA_G
Cuts at: 3004 3362 3004
Size: 358 4375
BstAPI GCAn_nnn'nTGC
Cuts at: 2110 2110
Size: 4733
BstBI TT'CG_AA
Cuts at: 3094 3094
Size: 4733
BstYI r'GATC_y
Cuts at: 1 273 1758 2225 2248 2583 2829 3841 3927
Size: 272 1485 467 23 335 246 1012 86
Cuts at: 3927 3939 4285 1
Size: 12 346 449
Fragments arranged by size:
1485 1012 467 449 346 335 272 246
86 23 12
BtgI C'CryG_G
Cuts at: 128 743 857 1295 128
Size: 615 114 438 3566
Fragments arranged by size:
3566 615 438 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2683 2864 3027 2683
Size: 181 163 4389
Fragments arranged by size:
4389 181 163
BtsI GCAGTG_nn'
Cuts at: 1976 1976
Size: 4733
DraI TTT'AAA
Cuts at: 1732 1732
Size: 4733
DraIII CAC_nnn'GTG
Cuts at: 558 558
Size: 4733
DrdI GACnn_nn'nnGTC
Cuts at: 2439 3297 2439
Size: 858 3875
EaeI y'GGCC_r
Cuts at: 125 450 867 924 1292 1301 1593 2318 2492
Size: 325 417 57 368 9 292 725 174
Cuts at: 2492 2883 2910 4255 125
Size: 391 27 1345 603
Fragments arranged by size:
1345 725 603 417 391 368 325 292
174 57 27 9
EagI C'GGCC_G
Cuts at: 2318 2318
Size: 4733
EarI CTCTTCn'nnn_
Cuts at: 1501 2756 2966 1501
Size: 1255 210 3268
Fragments arranged by size:
3268 1255 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1012 2941 3251 3397 4543 1012
Size: 1929 310 146 1146 1202
Fragments arranged by size:
1929 1202 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1745 1745
Size: 4733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 702 856 898 1142 1735 2558 2990 3737 4005
Size: 154 42 244 593 823 432 747 268
Cuts at: 4005 702
Size: 1430
Fragments arranged by size:
1430 823 747 593 432 268 244 154
42
EcoNI CCTnn'n_nnAGG
Cuts at: 1453 1714 1453
Size: 261 4472
EcoO109I rG'GnC_Cy
Cuts at: 165 166 207 231 305 843 853 1017 1424
Size: 1 41 24 74 538 10 164 407
Cuts at: 1424 1872 165
Size: 448 3026
Fragments arranged by size:
3026 538 448 407 164 74 41 24
10 1
EcoRI G'AATT_C
Cuts at: 1737 1737
Size: 4733
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 516 2752 516
Size: 2236 2497
FspI TGC'GCA
Cuts at: 2514 2514
Size: 4733
HaeII r_GCGC'y
Cuts at: 835 2167 2415 3437 4416 835
Size: 1332 248 1022 979 1152
Fragments arranged by size:
1332 1152 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 871 903 1680 1712 2575 2605 2607 2637 871
Size: 32 777 32 863 30 2 30 2967
Fragments arranged by size:
2967 863 777 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1772 1772
Size: 4733
KasI G'GCGC_C
Cuts at: 2163 2411 2163
Size: 248 4485
KpnI G_GTAC'C
Cuts at: 1753 1753
Size: 4733
MluI A'CGCG_T
Cuts at: 4166 4166
Size: 4733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 301 1562 1588 3404 3588 301
Size: 1261 26 1816 184 1446
Fragments arranged by size:
1816 1446 1261 184 26
MscI TGG'CCA
Cuts at: 127 869 1595 2494 127
Size: 742 726 899 2366
Fragments arranged by size:
2366 899 742 726
MslI CAynn'nnrTG
Cuts at: 1288 1294 1343 2722 2849 1288
Size: 6 49 1379 127 3172
Fragments arranged by size:
3172 1379 127 49 6
MspA1I CmG'CkG
Cuts at: 226 387 428 1104 1110 1396 1844 2158 2518
Size: 161 41 676 6 286 448 314 360
Cuts at: 2518 3531 3776 226
Size: 1013 245 1183
Fragments arranged by size:
1183 1013 676 448 360 314 286 245
161 41 6
NaeI GCC'GGC
Cuts at: 187 2914 187
Size: 2727 2006
NarI GG'CG_CC
Cuts at: 2164 2412 2164
Size: 248 4485
NcoI C'CATG_G
Cuts at: 128 743 128
Size: 615 4118
NgoMIV G'CCGG_C
Cuts at: 185 2912 185
Size: 2727 2006
NspI r_CATG'y
Cuts at: 284 984 1446 1786 2286 3193 284
Size: 700 462 340 500 907 1824
Fragments arranged by size:
1824 907 700 500 462 340
PasI CC'CwG_GG
Cuts at: 149 780 1464 1614 149
Size: 631 684 150 3268
Fragments arranged by size:
3268 684 631 150
PciI A'CATG_T
Cuts at: 280 1442 3189 280
Size: 1162 1747 1824
Fragments arranged by size:
1824 1747 1162
PflMI CCAn_nnn'nTGG
Cuts at: 876 1342 1519 1602 876
Size: 466 177 83 4007
Fragments arranged by size:
4007 466 177 83
PmeI GTTT'AAAC
Cuts at: 1732 1732
Size: 4733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 4701
PpuMI rG'GwC_Cy
Cuts at: 207 305 207
Size: 98 4635
PspOMI G'GGCC_C
Cuts at: 165 1424 165
Size: 1259 3474
PstI C_TGCA'G
Cuts at: 968 1025 1109 1780 2465 968
Size: 57 84 671 685 3236
Fragments arranged by size:
3236 685 671 84 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4733
PvuII CAG'CTG
Cuts at: 387 1104 1110 1396 2158 2518 387
Size: 717 6 286 762 360 2602
Fragments arranged by size:
2602 762 717 360 286 6
RsrII CG'GwC_CG
Cuts at: 2928 2928
Size: 4733
SacI G_AGCT'C
Cuts at: 1747 1747
Size: 4733
SalI G'TCGA_C
Cuts at: 1770 1770
Size: 4733
SanDI GG'GwC_CC
Cuts at: 305 305
Size: 4733
SapI GCTCTTCn'nnn_
Cuts at: 1501 2756 2966 1501
Size: 1255 210 3268
Fragments arranged by size:
3268 1255 210
SbfI CC_TGCA'GG
Cuts at: 1025 1780 1025
Size: 755 3978
ScaI AGT'ACT
Cuts at: 4250 4250
Size: 4733
SfcI C'TryA_G
Cuts at: 33 964 1021 1105 1776 2461 3454 3645 33
Size: 931 57 84 671 685 993 191 1121
Fragments arranged by size:
1121 993 931 685 671 191 84 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 238 1300 238
Size: 1062 3671
SfoI GGC'GCC
Cuts at: 2165 2413 2165
Size: 248 4485
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
SmaI CCC'GGG
Cuts at: 303 569 1755 303
Size: 266 1186 3281
Fragments arranged by size:
3281 1186 266
SmlI C'TyrA_G
Cuts at: 235 1093 1888 2803 3295 3557 235
Size: 858 795 915 492 262 1411
Fragments arranged by size:
1411 915 858 795 492 262
SpeI A'CTAG_T
Cuts at: 67 1315 67
Size: 1248 3485
SphI G_CATG'C
Cuts at: 1786 2286 1786
Size: 500 4233
StuI AGG'CCT
Cuts at: 823 823
Size: 4733
StyI C'CwwG_G
Cuts at: 128 743 824 1867 2221 128
Size: 615 81 1043 354 2640
Fragments arranged by size:
2640 1043 615 354 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1091 1441 2316 2449 2908 3117 1091
Size: 350 875 133 459 209 2707
Fragments arranged by size:
2707 875 459 350 209 133
TatI w'GTAC_w
Cuts at: 575 591 1133 4248 575
Size: 16 542 3115 1060
Fragments arranged by size:
3115 1060 542 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1678 1710 4250 4282 1678
Size: 32 2540 32 2129
Fragments arranged by size:
2540 2129 32 32
Tth111I GACn'n_nGTC
Cuts at: 1531 2530 1531
Size: 999 3734
XbaI T'CTAG_A
Cuts at: 1764 1764
Size: 4733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 750 1293 2228 750
Size: 543 935 3255
Fragments arranged by size:
3255 935 543
XmaI C'CCGG_G
Cuts at: 301 567 1753 301
Size: 266 1186 3281
Fragments arranged by size:
3281 1186 266
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI