Restriction map for
pF1KB3048
(Circular) (Six-base) MAPSORT of: pF1KB3048.seq Check: 6482 from: 1 to: 7139
pF1KB3048 7139 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3910 3910
Size: 7139
AatII G_ACGT'C
Cuts at: 1868 1868
Size: 7139
AccI GT'mk_AC
Cuts at: 770 3489 3533 3769 4177 770
Size: 2719 44 236 408 3732
Fragments arranged by size:
3732 2719 408 236 44
Acc65I G'GTAC_C
Cuts at: 886 1931 2294 4155 886
Size: 1045 363 1861 3870
Fragments arranged by size:
3870 1861 1045 363
AclI AA'CG_TT
Cuts at: 6979 6979
Size: 7139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 1556 2256 3236 3691 3741 4964 5396 6143
Size: 933 700 980 455 50 1223 432 747
Cuts at: 6143 6411 623
Size: 268 1351
Fragments arranged by size:
1351 1223 980 933 747 700 455 432
268 50
AfeI AGC'GCT
Cuts at: 842 842
Size: 7139
AflIII A'CryG_T
Cuts at: 2150 2566 2692 3572 4491 5595 6572 2150
Size: 416 126 880 919 1104 977 2717
Fragments arranged by size:
2717 1104 977 919 880 416 126
AgeI A'CCGG_T
Cuts at: 5527 5527
Size: 7139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1650 3267 1650
Size: 1617 5522
AlwNI CAG_nnn'CTG
Cuts at: 3044 4444 6011 3044
Size: 1400 1567 4172
Fragments arranged by size:
4172 1567 1400
ApaI G_GGCC'C
Cuts at: 1163 1264 1163
Size: 101 7038
ApaLI G'TGCA_C
Cuts at: 2277 5909 2277
Size: 3632 3507
ApoI r'AATT_y
Cuts at: 399 559 2013 4107 4143 6492 6748 399
Size: 160 1454 2094 36 2349 256 790
Fragments arranged by size:
2349 2094 1454 790 256 160 36
AseI AT'TA_AT
Cuts at: 20 1010 2504 2579 20
Size: 990 1494 75 4580
Fragments arranged by size:
4580 1494 990 75
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7139
AvaI C'yCGr_G
Cuts at: 1331 4159 1331
Size: 2828 4311
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2278 2311 2278
Size: 33 7106
BamHI G'GATC_C
Cuts at: 1104 4164 1104
Size: 3060 4079
BanI G'GyrC_C
Cuts at: 436 886 1931 2294 4155 4569 4817 4852 436
Size: 450 1045 363 1861 414 248 35 2723
Fragments arranged by size:
2723 1861 1045 450 414 363 248 35
BanII G_rGCy'C
Cuts at: 1163 1264 2269 2392 2603 3433 4153 5183 1163
Size: 101 1005 123 211 830 720 1030 3119
Fragments arranged by size:
3119 1030 1005 830 720 211 123 101
BbeI G_GCGC'C
Cuts at: 4573 4821 4573
Size: 248 6891
BbsI GAAGACnn'nnnn_
Cuts at: 664 724 1194 2333 664
Size: 60 470 1139 5470
Fragments arranged by size:
5470 1139 470 60
BbvCI CC'TCA_GC
Cuts at: 3836 3836
Size: 7139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1064 1098 1064
Size: 34 7105
BciVI GTATCCnnnnn_n'
Cuts at: 105 937 3924 5037 5798 7139 105
Size: 832 2987 1113 761 1341 105
Fragments arranged by size:
2987 1341 1113 832 761 105
BclI T'GATC_A
Cuts at: 4659 4659
Size: 7139
BglI GCCn_nnn'nGGC
Cuts at: 650 2168 650
Size: 1518 5621
BglII A'GATC_T
Cuts at: 4654 6691 4654
Size: 2037 5102
BlpI GC'TnA_GC
Cuts at: 3692 4251 3692
Size: 559 6580
Bme1580I G_kGCm'C
Cuts at: 394 1163 1264 1883 2281 3660 4764 4857 5913
Size: 769 101 619 398 1379 1104 93 1056
Cuts at: 5913 394
Size: 1620
Fragments arranged by size:
1620 1379 1104 1056 769 619 398 101
93
BmgBI CAC'GTC
Cuts at: 768 768
Size: 7139
BmrI ACTGGGnnnn_n'
Cuts at: 421 2951 3386 4351 4432 4767 6911 421
Size: 2530 435 965 81 335 2144 649
Fragments arranged by size:
2530 2144 965 649 435 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5506 5506
Size: 7139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1433 3221 3758 4150 1433
Size: 1788 537 392 4422
Fragments arranged by size:
4422 1788 537 392
Bpu10I CC'TnA_GC
Cuts at: 3836 3836
Size: 7139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 1562 4315 5194 5686 5984 1444
Size: 118 2753 879 492 298 2599
Fragments arranged by size:
2753 2599 879 492 298 118
BsaI GGTCTCn'nnnn_
Cuts at: 2432 6843 2432
Size: 4411 2728
BsaAI yAC'GTr
Cuts at: 2693 3573 4059 4492 2693
Size: 880 486 433 5340
Fragments arranged by size:
5340 880 486 433
BsaBI GATnn'nnATC
Cuts at: 6 2987 3920 4653 6690 6
Size: 2981 933 733 2037 455
Fragments arranged by size:
2981 2037 933 733 455
BsaHI Gr'CG_yC
Cuts at: 1865 3035 3720 3743 4570 4818 5520 7016 1865
Size: 1170 685 23 827 248 702 1496 1988
Fragments arranged by size:
1988 1496 1170 827 702 685 248 23
BsaWI w'CCGG_w
Cuts at: 1907 2184 2535 3983 4328 4849 5527 5801 5948
Size: 277 351 1448 345 521 678 274 147
Cuts at: 5948 6359 1907
Size: 411 2687
Fragments arranged by size:
2687 1448 678 521 411 351 345 277
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 539 569 1494 1524 1732 1762 2166 2196 539
Size: 30 925 30 208 30 404 30 5482
Fragments arranged by size:
5482 925 404 208 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1834 2507 3838 1834
Size: 673 1331 5135
Fragments arranged by size:
5135 1331 673
BseYI C'CCAG_C
Cuts at: 467 1439 1797 3548 4564 5322 5899 6395 467
Size: 972 358 1751 1016 758 577 496 1211
Fragments arranged by size:
1751 1211 1016 972 758 577 496 358
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 387 1358 2028 2394 4068 207
Size: 180 971 670 366 1674 3278
Fragments arranged by size:
3278 1674 971 670 366 180
BsiEI CG_ry'CG
Cuts at: 104 3496 4681 4727 5935 104
Size: 3392 1185 46 1208 1308
Fragments arranged by size:
3392 1308 1208 1185 46
BsiHKAI G_wGCw'C
Cuts at: 2281 2364 4153 4931 5121 5913 2281
Size: 83 1789 778 190 792 3507
Fragments arranged by size:
3507 1789 792 778 190 83
BsiWI C'GTAC_G
Cuts at: 2689 2689
Size: 7139
BsmI GAATG_Cn'
Cuts at: 462 967 1855 2344 2467 3019 3088 3256 3322
Size: 505 888 489 123 552 69 168 66
Cuts at: 3322 3847 3970 4008 462
Size: 525 123 38 3593
Fragments arranged by size:
3593 888 552 525 505 489 168 123
123 69 66 38
Bsp1286I G_dGCh'C
Cuts at: 394 1163 1264 1883 2269 2281 2364 2392 2603
Size: 769 101 619 386 12 83 28 211
Cuts at: 2603 3433 3660 4153 4764 4857 4931 5121 5183
Size: 830 227 493 611 93 74 190 62
Cuts at: 5183 5913 394
Size: 730 1620
Fragments arranged by size:
1620 830 769 730 619 611 493 386
227 211 190 101 93 83 74 62
28 12
BspEI T'CCGG_A
Cuts at: 2184 2535 6359 2184
Size: 351 3824 2964
Fragments arranged by size:
3824 2964 351
BspHI T'CATG_A
Cuts at: 6315 6315
Size: 7139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 229 744 1090 3910 4189 4403 4705 5086 229
Size: 515 346 2820 279 214 302 381 2282
Fragments arranged by size:
2820 2282 515 381 346 302 279 214
BsrBI CCG'CTC
Cuts at: 50 156 2954 5431 5485 6969 7134 50
Size: 106 2798 2477 54 1484 165 55
Fragments arranged by size:
2798 2477 1484 165 106 55 54
BsrDI GCAATG_nn'
Cuts at: 5051 5051
Size: 7139
BsrFI r'CCGG_y
Cuts at: 800 3495 3581 3722 5137 5318 5527 800
Size: 2695 86 141 1415 181 209 2412
Fragments arranged by size:
2695 2412 1415 209 181 141 86
BsrGI T'GTAC_A
Cuts at: 3091 3091
Size: 7139
BssSI C'ACGA_G
Cuts at: 5410 5768 5410
Size: 358 6781
BstAPI GCAn_nnn'nTGC
Cuts at: 4516 4516
Size: 7139
BstBI TT'CG_AA
Cuts at: 5500 5500
Size: 7139
BstXI CCAn_nnnn'nTGG
Cuts at: 3465 3465
Size: 7139
BstYI r'GATC_y
Cuts at: 1 422 1104 2023 4164 4631 4654 4989 5235
Size: 421 682 919 2141 467 23 335 246
Cuts at: 5235 6247 6333 6345 6691 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2141 1012 919 682 467 449 421 346
335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 3490 3770 3490
Size: 280 6859
Bsu36I CC'TnA_GG
Cuts at: 1855 1855
Size: 7139
BtgI C'CryG_G
Cuts at: 107 432 776 1061 3458 107
Size: 325 344 285 2397 3788
Fragments arranged by size:
3788 2397 344 325 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3983 5089 5270 5433 3983
Size: 1106 181 163 5689
Fragments arranged by size:
5689 1106 181 163
BtsI GCAGTG_nn'
Cuts at: 1632 2011 2254 2377 2953 3367 3658 4382 1632
Size: 379 243 123 576 414 291 724 4389
Fragments arranged by size:
4389 724 576 414 379 291 243 123
ClaI AT'CG_AT
Cuts at: 1074 1074
Size: 7139
DraI TTT'AAA
Cuts at: 4138 4138
Size: 7139
DraIII CAC_nnn'GTG
Cuts at: 832 1270 3617 832
Size: 438 2347 4354
Fragments arranged by size:
4354 2347 438
DrdI GACnn_nn'nnGTC
Cuts at: 4845 5703 4845
Size: 858 6281
EaeI y'GGCC_r
Cuts at: 177 311 360 798 1811 2685 3408 3935 4724
Size: 134 49 438 1013 874 723 527 789
Cuts at: 4724 4898 5289 5316 6661 177
Size: 174 391 27 1345 655
Fragments arranged by size:
1345 1013 874 789 723 655 527 438
391 174 134 49 27
EagI C'GGCC_G
Cuts at: 4724 4724
Size: 7139
EarI CTCTTCn'nnn_
Cuts at: 1145 1298 5162 5372 1145
Size: 153 3864 210 2912
Fragments arranged by size:
3864 2912 210 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1369 5347 5657 5803 6949 1369
Size: 3978 310 146 1146 1559
Fragments arranged by size:
3978 1559 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4151 4151
Size: 7139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 1433 1556 2256 3221 3236 3691 3741 3758
Size: 810 123 700 965 15 455 50 17
Cuts at: 3758 4150 4964 5396 6143 6411 623
Size: 392 814 432 747 268 1351
Fragments arranged by size:
1351 965 814 810 747 700 455 432
392 268 123 50 17 15
EcoO109I rG'GnC_Cy
Cuts at: 1159 1160 1260 1317 1901 2516 2891 4278 1159
Size: 1 100 57 584 615 375 1387 4020
Fragments arranged by size:
4020 1387 615 584 375 100 57 1
EcoRI G'AATT_C
Cuts at: 2013 4143 2013
Size: 2130 5009
EcoRV GAT'ATC
Cuts at: 1617 2454 1617
Size: 837 6302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 210 5158 210
Size: 4948 2191
FspI TGC'GCA
Cuts at: 2115 4920 2115
Size: 2805 4334
HaeII r_GCGC'y
Cuts at: 844 4573 4821 5843 6822 844
Size: 3729 248 1022 979 1161
Fragments arranged by size:
3729 1161 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 4981 5011 5013 5043 3257
Size: 32 1692 30 2 30 5353
Fragments arranged by size:
5353 1692 32 30 30 2
HincII GTy'rAC
Cuts at: 1435 1845 3753 4178 1435
Size: 410 1908 425 4396
Fragments arranged by size:
4396 1908 425 410
HindIII A'AGCT_T
Cuts at: 605 1538 605
Size: 933 6206
KasI G'GCGC_C
Cuts at: 4569 4817 4569
Size: 248 6891
KpnI G_GTAC'C
Cuts at: 890 1935 2298 4159 890
Size: 1045 363 1861 3870
Fragments arranged by size:
3870 1861 1045 363
MluI A'CGCG_T
Cuts at: 6572 6572
Size: 7139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1761 2156 5810 5994 1761
Size: 395 3654 184 2906
Fragments arranged by size:
3654 2906 395 184
MscI TGG'CCA
Cuts at: 362 1813 3410 4900 362
Size: 1451 1597 1490 2601
Fragments arranged by size:
2601 1597 1490 1451
MslI CAynn'nnrTG
Cuts at: 187 2357 3166 3560 3709 5128 5255 187
Size: 2170 809 394 149 1419 127 2071
Fragments arranged by size:
2170 2071 1419 809 394 149 127
MspA1I CmG'CkG
Cuts at: 236 248 1443 1760 1928 2301 2761 2774 3696
Size: 12 1195 317 168 373 460 13 922
Cuts at: 3696 4250 4564 4924 5937 6182 236
Size: 554 314 360 1013 245 1193
Fragments arranged by size:
1195 1193 1013 922 554 460 373 360
317 314 245 168 13 12
NaeI GCC'GGC
Cuts at: 3583 3724 5320 3583
Size: 141 1596 5402
Fragments arranged by size:
5402 1596 141
NarI GG'CG_CC
Cuts at: 4570 4818 4570
Size: 248 6891
NcoI C'CATG_G
Cuts at: 107 432 1061 3458 107
Size: 325 629 2397 3788
Fragments arranged by size:
3788 2397 629 325
NdeI CA'TA_TG
Cuts at: 3732 3732
Size: 7139
NgoMIV G'CCGG_C
Cuts at: 3581 3722 5318 3581
Size: 141 1596 5402
Fragments arranged by size:
5402 1596 141
NspI r_CATG'y
Cuts at: 499 2154 2570 3651 4192 4692 5599 499
Size: 1655 416 1081 541 500 907 2039
Fragments arranged by size:
2039 1655 1081 907 541 500 416
PasI CC'CwG_GG
Cuts at: 1752 1752
Size: 7139
PciI A'CATG_T
Cuts at: 2150 2566 5595 2150
Size: 416 3029 3694
Fragments arranged by size:
3694 3029 416
PflMI CCAn_nnn'nTGG
Cuts at: 433 957 2809 433
Size: 524 1852 4763
Fragments arranged by size:
4763 1852 524
PfoI T'CCnGG_A
Cuts at: 809 2019 2956 3464 3887 809
Size: 1210 937 508 423 4061
Fragments arranged by size:
4061 1210 937 508 423
PmeI GTTT'AAAC
Cuts at: 4138 4138
Size: 7139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6278 6310 6278
Size: 32 7107
PpuMI rG'GwC_Cy
Cuts at: 2516 2891 2516
Size: 375 6764
PshAI GACnn'nnGTC
Cuts at: 383 496 3038 3949 383
Size: 113 2542 911 3573
Fragments arranged by size:
3573 2542 911 113
PsiI TTA'TAA
Cuts at: 977 977
Size: 7139
PspOMI G'GGCC_C
Cuts at: 1159 1260 1159
Size: 101 7038
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 870 902 870
Size: 32 7107
PstI C_TGCA'G
Cuts at: 2514 2682 3362 4186 4871 2514
Size: 168 680 824 685 4782
Fragments arranged by size:
4782 824 685 680 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7139
PvuII CAG'CTG
Cuts at: 236 248 1443 2301 2761 3696 4564 4924 236
Size: 12 1195 858 460 935 868 360 2451
Fragments arranged by size:
2451 1195 935 868 858 460 360 12
RsrII CG'GwC_CG
Cuts at: 5334 5334
Size: 7139
SacI G_AGCT'C
Cuts at: 4153 4153
Size: 7139
SalI G'TCGA_C
Cuts at: 4176 4176
Size: 7139
SapI GCTCTTCn'nnn_
Cuts at: 1298 5162 5372 1298
Size: 3864 210 3065
Fragments arranged by size:
3864 3065 210
SbfI CC_TGCA'GG
Cuts at: 4186 4186
Size: 7139
ScaI AGT'ACT
Cuts at: 6656 6656
Size: 7139
SfcI C'TryA_G
Cuts at: 33 1680 2199 2510 2678 3358 4182 4867 5860
Size: 1647 519 311 168 680 824 685 993
Cuts at: 5860 6051 33
Size: 191 1121
Fragments arranged by size:
1647 1121 993 824 685 680 519 311
191 168
SfiI GGCCn_nnn'nGGCC
Cuts at: 650 650
Size: 7139
SfoI GGC'GCC
Cuts at: 4571 4819 4571
Size: 248 6891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7139
SmaI CCC'GGG
Cuts at: 1333 4161 1333
Size: 2828 4311
SmlI C'TyrA_G
Cuts at: 1423 1577 4294 5209 5701 5963 1423
Size: 154 2717 915 492 262 2599
Fragments arranged by size:
2717 2599 915 492 262 154
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7139
SphI G_CATG'C
Cuts at: 3651 4192 4692 3651
Size: 541 500 6098
Fragments arranged by size:
6098 541 500
StuI AGG'CCT
Cuts at: 1255 3640 1255
Size: 2385 4754
StyI C'CwwG_G
Cuts at: 107 227 432 699 1061 1298 3458 3746 4273
Size: 120 205 267 362 237 2160 288 527
Cuts at: 4273 4627 107
Size: 354 2619
Fragments arranged by size:
2619 2160 527 362 354 288 267 237
205 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4722 4855 5314 5523 4722
Size: 133 459 209 6338
Fragments arranged by size:
6338 459 209 133
TatI w'GTAC_w
Cuts at: 1600 1726 2527 2927 3091 3448 6654 1600
Size: 126 801 400 164 357 3206 2085
Fragments arranged by size:
3206 2085 801 400 357 164 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 539 571 1732 1764 2220 2252 6656 6688 539
Size: 32 1161 32 456 32 4404 32 990
Fragments arranged by size:
4404 1161 990 456 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4936 4936
Size: 7139
XbaI T'CTAG_A
Cuts at: 4170 4170
Size: 7139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 817 4634 817
Size: 3817 3322
XmaI C'CCGG_G
Cuts at: 1331 4159 1331
Size: 2828 4311
ZraI GAC'GTC
Cuts at: 1866 1866
Size: 7139
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AloI AscI AvrII BfrBI BmtI
BsmBI BssHII BstEII CspCI EcoNI FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PmlI PspXI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI