Restriction map for
pF1KB3047
(Circular) (Six-base) MAPSORT of: pF1KB3047.seq Check: 3289 from: 1 to: 6554
pF1KB3047 6554 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1770 1770
Size: 6554
AccI GT'mk_AC
Cuts at: 3007 3149 3592 3007
Size: 142 443 5969
Fragments arranged by size:
5969 443 142
Acc65I G'GTAC_C
Cuts at: 3570 3570
Size: 6554
AclI AA'CG_TT
Cuts at: 856 1401 6394 856
Size: 545 4993 1016
Fragments arranged by size:
4993 1016 545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1235 2216 4379 4811 5558 5826 1235
Size: 981 2163 432 747 268 1963
Fragments arranged by size:
2163 1963 981 747 432 268
AflIII A'CryG_T
Cuts at: 2696 2775 3906 5010 5987 2696
Size: 79 1131 1104 977 3263
Fragments arranged by size:
3263 1131 1104 977 79
AgeI A'CCGG_T
Cuts at: 4942 4942
Size: 6554
AleI CACnn'nnGTG
Cuts at: 1354 1354
Size: 6554
AlwNI CAG_nnn'CTG
Cuts at: 3039 3233 3859 5426 3039
Size: 194 626 1567 4167
Fragments arranged by size:
4167 1567 626 194
ApaI G_GGCC'C
Cuts at: 2647 2647
Size: 6554
ApaLI G'TGCA_C
Cuts at: 2570 5324 2570
Size: 2754 3800
ApoI r'AATT_y
Cuts at: 121 398 1818 1854 2005 2303 2339 3558 5907
Size: 277 1420 36 151 298 36 1219 2349
Cuts at: 5907 6163 121
Size: 256 512
Fragments arranged by size:
2349 1420 1219 512 298 277 256 151
36 36
AseI AT'TA_AT
Cuts at: 20 2314 20
Size: 2294 4260
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6554
AvaI C'yCGr_G
Cuts at: 2429 3574 2429
Size: 1145 5409
AvrII C'CTAG_G
Cuts at: 958 958
Size: 6554
BamHI G'GATC_C
Cuts at: 411 3267 3579 411
Size: 2856 312 3386
Fragments arranged by size:
3386 2856 312
BanI G'GyrC_C
Cuts at: 3570 3984 4232 4267 3570
Size: 414 248 35 5857
Fragments arranged by size:
5857 414 248 35
BanII G_rGCy'C
Cuts at: 1093 1294 2647 3518 3568 4598 1093
Size: 201 1353 871 50 1030 3049
Fragments arranged by size:
3049 1353 1030 871 201 50
BbeI G_GCGC'C
Cuts at: 3988 4236 3988
Size: 248 6306
BbsI GAAGACnn'nnnn_
Cuts at: 2893 3209 2893
Size: 316 6238
BbvCI CC'TCA_GC
Cuts at: 1787 1787
Size: 6554
BciVI GTATCCnnnnn_n'
Cuts at: 2104 4452 5213 6554 2104
Size: 2348 761 1341 2104
Fragments arranged by size:
2348 2104 1341 761
BclI T'GATC_A
Cuts at: 3461 4074 3461
Size: 613 5941
BglII A'GATC_T
Cuts at: 496 634 2718 4069 6106 496
Size: 138 2084 1351 2037 944
Fragments arranged by size:
2084 2037 1351 944 138
BlpI GC'TnA_GC
Cuts at: 3666 3666
Size: 6554
Bme1580I G_kGCm'C
Cuts at: 2574 2647 4179 4272 5328 2574
Size: 73 1532 93 1056 3800
Fragments arranged by size:
3800 1532 1056 93 73
BmrI ACTGGGnnnn_n'
Cuts at: 2961 3766 3847 4182 6326 2961
Size: 805 81 335 2144 3189
Fragments arranged by size:
3189 2144 805 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4921 4921
Size: 6554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1172 1381 1636 2480 2699 2916 2960 3054
Size: 400 209 255 844 219 217 44 94
Cuts at: 3054 772
Size: 4272
Fragments arranged by size:
4272 844 400 255 219 217 209 94
44
Bpu10I CC'TnA_GC
Cuts at: 1787 1787
Size: 6554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1876 3730 4609 5101 5399 1876
Size: 1854 879 492 298 3031
Fragments arranged by size:
3031 1854 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6258 6258
Size: 6554
BsaAI yAC'GTr
Cuts at: 2121 2778 3907 2121
Size: 657 1129 4768
Fragments arranged by size:
4768 1129 657
BsaBI GATnn'nnATC
Cuts at: 6 2369 4068 6105 6
Size: 2363 1699 2037 455
Fragments arranged by size:
2363 2037 1699 455
BsaHI Gr'CG_yC
Cuts at: 3985 4233 4935 6431 3985
Size: 248 702 1496 4108
Fragments arranged by size:
4108 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2879 3743 4264 4942 5216 5363 5774 2879
Size: 864 521 678 274 147 411 3659
Fragments arranged by size:
3659 864 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 745 775 1149 1179 1934 1964 745
Size: 30 374 30 755 30 5335
Fragments arranged by size:
5335 755 374 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 1804 2698 343
Size: 1461 894 4199
Fragments arranged by size:
4199 1461 894
BseYI C'CCAG_C
Cuts at: 2991 3233 3979 4737 5314 5810 2991
Size: 242 746 758 577 496 3735
Fragments arranged by size:
3735 758 746 577 496 242
BsiEI CG_ry'CG
Cuts at: 104 4096 4142 5350 104
Size: 3992 46 1208 1308
Fragments arranged by size:
3992 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1093 2574 3568 4346 4536 5328 1093
Size: 1481 994 778 190 792 2319
Fragments arranged by size:
2319 1481 994 792 778 190
BsmI GAATG_Cn'
Cuts at: 2136 2136
Size: 6554
BsmBI CGTCTCn'nnnn_
Cuts at: 325 325
Size: 6554
Bsp1286I G_dGCh'C
Cuts at: 1093 1294 2574 2647 3518 3568 4179 4272 4346
Size: 201 1280 73 871 50 611 93 74
Cuts at: 4346 4536 4598 5328 1093
Size: 190 62 730 2319
Fragments arranged by size:
2319 1280 871 730 611 201 190 93
74 73 62 50
BspEI T'CCGG_A
Cuts at: 2879 5774 2879
Size: 2895 3659
BspHI T'CATG_A
Cuts at: 2730 5730 2730
Size: 3000 3554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 330 1770 3604 3818 4120 4501 330
Size: 1440 1834 214 302 381 2383
Fragments arranged by size:
2383 1834 1440 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4846 4900 6384 6549 50
Size: 4796 54 1484 165 55
Fragments arranged by size:
4796 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2292 2367 4466 2292
Size: 75 2099 4380
Fragments arranged by size:
4380 2099 75
BsrFI r'CCGG_y
Cuts at: 4552 4733 4942 4552
Size: 181 209 6164
Fragments arranged by size:
6164 209 181
BsrGI T'GTAC_A
Cuts at: 3123 3123
Size: 6554
BssSI C'ACGA_G
Cuts at: 4825 5183 4825
Size: 358 6196
BstAPI GCAn_nnn'nTGC
Cuts at: 2820 3931 2820
Size: 1111 5443
BstBI TT'CG_AA
Cuts at: 3541 4915 3541
Size: 1374 5180
BstEII G'GTnAC_C
Cuts at: 1915 2857 1915
Size: 942 5612
BstXI CCAn_nnnn'nTGG
Cuts at: 1287 1287
Size: 6554
BstYI r'GATC_y
Cuts at: 1 411 496 634 2718 2876 3267 3579 4046
Size: 410 85 138 2084 158 391 312 467
Cuts at: 4046 4069 4404 4650 5662 5748 5760 6106 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2084 1012 467 449 410 391 346 335
312 246 158 138 86 85 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 6554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 699 2867 4504 4685 4848 699
Size: 2168 1637 181 163 2405
Fragments arranged by size:
2405 2168 1637 181 163
BtsI GCAGTG_nn'
Cuts at: 1842 3797 1842
Size: 1955 4599
DraI TTT'AAA
Cuts at: 209 2037 2472 2483 3553 209
Size: 1828 435 11 1070 3210
Fragments arranged by size:
3210 1828 1070 435 11
DrdI GACnn_nn'nnGTC
Cuts at: 4260 5118 4260
Size: 858 5696
EaeI y'GGCC_r
Cuts at: 4139 4313 4704 4731 6076 4139
Size: 174 391 27 1345 4617
Fragments arranged by size:
4617 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4139 4139
Size: 6554
EarI CTCTTCn'nnn_
Cuts at: 770 1044 1079 2235 2758 4577 4787 770
Size: 274 35 1156 523 1819 210 2537
Fragments arranged by size:
2537 1819 1156 523 274 210 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4762 5072 5218 6364 4762
Size: 310 146 1146 4952
Fragments arranged by size:
4952 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1091 3566 1091
Size: 2475 4079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1172 1235 1381 1636 2216 2480 2699 2916
Size: 400 63 146 255 580 264 219 217
Cuts at: 2916 2960 3054 4379 4811 5558 5826 772
Size: 44 94 1325 432 747 268 1500
Fragments arranged by size:
1500 1325 747 580 432 400 268 264
255 219 217 146 94 63 44
EcoO109I rG'GnC_Cy
Cuts at: 1276 2056 3036 3693 1276
Size: 780 980 657 4137
Fragments arranged by size:
4137 980 780 657
EcoRI G'AATT_C
Cuts at: 2303 3558 2303
Size: 1255 5299
EcoRV GAT'ATC
Cuts at: 2535 2634 2535
Size: 99 6455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3334 4573 3334
Size: 1239 5315
FspI TGC'GCA
Cuts at: 2912 4335 2912
Size: 1423 5131
HaeII r_GCGC'y
Cuts at: 3988 4236 5258 6237 3988
Size: 248 1022 979 4305
Fragments arranged by size:
4305 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 307 339 939 971 1149 1181 1372 1404 2011
Size: 32 600 32 178 32 191 32 607
Cuts at: 2011 2043 2373 2405 2478 2510 3085 3117 3403
Size: 32 330 32 73 32 575 32 286
Cuts at: 3403 3435 4396 4426 4428 4458 307
Size: 32 961 30 2 30 2403
Fragments arranged by size:
2403 961 607 600 575 330 286 191
178 73 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3593 3593
Size: 6554
HindIII A'AGCT_T
Cuts at: 1247 1247
Size: 6554
KasI G'GCGC_C
Cuts at: 3984 4232 3984
Size: 248 6306
KpnI G_GTAC'C
Cuts at: 3574 3574
Size: 6554
MluI A'CGCG_T
Cuts at: 5987 5987
Size: 6554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 782 5225 5409 782
Size: 4443 184 1927
Fragments arranged by size:
4443 1927 184
MscI TGG'CCA
Cuts at: 4315 4315
Size: 6554
MslI CAynn'nnrTG
Cuts at: 770 1354 2776 4543 4670 770
Size: 584 1422 1767 127 2654
Fragments arranged by size:
2654 1767 1422 584 127
MspA1I CmG'CkG
Cuts at: 1018 2677 3233 3665 3979 4339 5352 5597 1018
Size: 1659 556 432 314 360 1013 245 1975
Fragments arranged by size:
1975 1659 1013 556 432 360 314 245
NaeI GCC'GGC
Cuts at: 4735 4735
Size: 6554
NarI GG'CG_CC
Cuts at: 3985 4233 3985
Size: 248 6306
NcoI C'CATG_G
Cuts at: 107 107
Size: 6554
NdeI CA'TA_TG
Cuts at: 2848 3050 2848
Size: 202 6352
NgoMIV G'CCGG_C
Cuts at: 4733 4733
Size: 6554
NspI r_CATG'y
Cuts at: 2251 2700 3607 4107 5014 2251
Size: 449 907 500 907 3791
Fragments arranged by size:
3791 907 907 500 449
PciI A'CATG_T
Cuts at: 2696 5010 2696
Size: 2314 4240
PfoI T'CCnGG_A
Cuts at: 2759 2937 2759
Size: 178 6376
PmeI GTTT'AAAC
Cuts at: 3553 3553
Size: 6554
PmlI CAC'GTG
Cuts at: 2778 2778
Size: 6554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5693 5725 5693
Size: 32 6522
PpuMI rG'GwC_Cy
Cuts at: 1276 3036 1276
Size: 1760 4794
PsiI TTA'TAA
Cuts at: 1097 1097
Size: 6554
PspOMI G'GGCC_C
Cuts at: 2643 2643
Size: 6554
PstI C_TGCA'G
Cuts at: 3601 4286 3601
Size: 685 5869
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6554
PvuII CAG'CTG
Cuts at: 1018 2677 3233 3979 4339 1018
Size: 1659 556 746 360 3233
Fragments arranged by size:
3233 1659 746 556 360
RsrII CG'GwC_CG
Cuts at: 4749 4749
Size: 6554
SacI G_AGCT'C
Cuts at: 1093 3568 1093
Size: 2475 4079
SalI G'TCGA_C
Cuts at: 3591 3591
Size: 6554
SapI GCTCTTCn'nnn_
Cuts at: 4577 4787 4577
Size: 210 6344
SbfI CC_TGCA'GG
Cuts at: 3601 3601
Size: 6554
ScaI AGT'ACT
Cuts at: 602 6071 602
Size: 5469 1085
SexAI A'CCwGG_T
Cuts at: 390 390
Size: 6554
SfcI C'TryA_G
Cuts at: 33 951 2786 3008 3443 3597 4282 5275 5466
Size: 918 1835 222 435 154 685 993 191
Cuts at: 5466 33
Size: 1121
Fragments arranged by size:
1835 1121 993 918 685 435 222 191
154
SfoI GGC'GCC
Cuts at: 3986 4234 3986
Size: 248 6306
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6554
SmaI CCC'GGG
Cuts at: 3576 3576
Size: 6554
SmlI C'TyrA_G
Cuts at: 1891 3709 4624 5116 5378 1891
Size: 1818 915 492 262 3067
Fragments arranged by size:
3067 1818 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6554
SphI G_CATG'C
Cuts at: 3607 4107 3607
Size: 500 6054
StuI AGG'CCT
Cuts at: 1334 1334
Size: 6554
StyI C'CwwG_G
Cuts at: 107 958 1046 1280 1499 1641 3272 3688 4042
Size: 851 88 234 219 142 1631 416 354
Cuts at: 4042 107
Size: 2619
Fragments arranged by size:
2619 1631 851 416 354 234 219 142
88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 606 1854 4137 4270 4729 4938 606
Size: 1248 2283 133 459 209 2222
Fragments arranged by size:
2283 2222 1248 459 209 133
TatI w'GTAC_w
Cuts at: 600 3123 3156 6069 600
Size: 2523 33 2913 1085
Fragments arranged by size:
2913 2523 1085 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6071 6103 6071
Size: 32 6522
Tth111I GACn'n_nGTC
Cuts at: 4351 4351
Size: 6554
XbaI T'CTAG_A
Cuts at: 3352 3585 3352
Size: 233 6321
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1059 2088 4049 1059
Size: 1029 1961 3564
Fragments arranged by size:
3564 1961 1029
XmaI C'CCGG_G
Cuts at: 3574 3574
Size: 6554
XmnI GAAnn'nnTTC
Cuts at: 3537 3537
Size: 6554
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI BaeI
BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PshAI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI