Restriction map for
pF1KB3046
(Circular) (Six-base) MAPSORT of: pF1KB3046.seq Check: 3146 from: 1 to: 7634
pF1KB3046 7634 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3715 3715
Size: 7634
AccI GT'mk_AC
Cuts at: 797 1574 4672 797
Size: 777 3098 3759
Fragments arranged by size:
3759 3098 777
Acc65I G'GTAC_C
Cuts at: 365 658 4650 365
Size: 293 3992 3349
Fragments arranged by size:
3992 3349 293
AclI AA'CG_TT
Cuts at: 7474 7474
Size: 7634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 869 1721 1870 3445 4571 5459 5891 6638
Size: 679 852 149 1575 1126 888 432 747
Cuts at: 6638 6906 190
Size: 268 918
Fragments arranged by size:
1575 1126 918 888 852 747 679 432
268 149
AflIII A'CryG_T
Cuts at: 2674 2711 3711 4986 6090 7067 2674
Size: 37 1000 1275 1104 977 3241
Fragments arranged by size:
3241 1275 1104 1000 977 37
AgeI A'CCGG_T
Cuts at: 6022 6022
Size: 7634
AhdI GACnn_n'nnGTC
Cuts at: 2712 2712
Size: 7634
AleI CACnn'nnGTG
Cuts at: 784 2108 3970 4348 784
Size: 1324 1862 378 4070
Fragments arranged by size:
4070 1862 1324 378
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4628 4628
Size: 7634
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2374 2406 2845 2877 2374
Size: 32 439 32 7131
Fragments arranged by size:
7131 439 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4939 6506 4939
Size: 1567 6067
ApaI G_GGCC'C
Cuts at: 3648 3648
Size: 7634
ApaLI G'TGCA_C
Cuts at: 853 6404 853
Size: 5551 2083
ApoI r'AATT_y
Cuts at: 217 679 723 1447 1985 3100 3478 3577 3631
Size: 462 44 724 538 1115 378 99 54
Cuts at: 3631 4459 4638 6987 7243 217
Size: 828 179 2349 256 608
Fragments arranged by size:
2349 1115 828 724 608 538 462 378
256 179 99 54 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 7634
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7634
AvaI C'yCGr_G
Cuts at: 1272 1382 3647 3735 4654 1272
Size: 110 2265 88 919 4252
Fragments arranged by size:
4252 2265 919 110 88
AvrII C'CTAG_G
Cuts at: 2355 2355
Size: 7634
BamHI G'GATC_C
Cuts at: 708 4659 708
Size: 3951 3683
BanI G'GyrC_C
Cuts at: 365 658 1042 3438 4650 5064 5312 5347 365
Size: 293 384 2396 1212 414 248 35 2652
Fragments arranged by size:
2652 2396 1212 414 384 293 248 35
BanII G_rGCy'C
Cuts at: 447 2810 2897 3648 4648 5678 447
Size: 2363 87 751 1000 1030 2403
Fragments arranged by size:
2403 2363 1030 1000 751 87
BbeI G_GCGC'C
Cuts at: 5068 5316 5068
Size: 248 7386
BbsI GAAGACnn'nnnn_
Cuts at: 178 1769 2404 3160 178
Size: 1591 635 756 4652
Fragments arranged by size:
4652 1591 756 635
BbvCI CC'TCA_GC
Cuts at: 734 1954 2920 3386 734
Size: 1220 966 466 4982
Fragments arranged by size:
4982 1220 966 466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1814 1848 4549 4583 90
Size: 34 1690 34 2701 34 3141
Fragments arranged by size:
3141 2701 1690 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1560 2400 3136 5532 6293 7634 1560
Size: 840 736 2396 761 1341 1560
Fragments arranged by size:
2396 1560 1341 840 761 736
BclI T'GATC_A
Cuts at: 5154 5154
Size: 7634
BfrBI ATG'CAT
Cuts at: 1224 1224
Size: 7634
BglI GCCn_nnn'nGGC
Cuts at: 2201 2201
Size: 7634
BglII A'GATC_T
Cuts at: 2640 5149 7186 2640
Size: 2509 2037 3088
Fragments arranged by size:
3088 2509 2037
BlpI GC'TnA_GC
Cuts at: 4746 4746
Size: 7634
Bme1580I G_kGCm'C
Cuts at: 857 3361 3648 5259 5352 6408 857
Size: 2504 287 1611 93 1056 2083
Fragments arranged by size:
2504 2083 1611 1056 287 93
BmrI ACTGGGnnnn_n'
Cuts at: 481 1870 1912 3610 4342 4846 4927 5262 7406
Size: 1389 42 1698 732 504 81 335 2144
Cuts at: 7406 481
Size: 709
Fragments arranged by size:
2144 1698 1389 732 709 504 335 81
42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1720 3411 3926 6001 1720
Size: 1691 515 2075 3353
Fragments arranged by size:
3353 2075 1691 515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 4145 1463
Size: 2682 4952
Bpu10I CC'TnA_GC
Cuts at: 734 1917 1954 2920 3386 3434 734
Size: 1183 37 966 466 48 4934
Fragments arranged by size:
4934 1183 966 466 48 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1451 1690 3921 4349 4810 5689 6181 6479 1451
Size: 239 2231 428 461 879 492 298 2606
Fragments arranged by size:
2606 2231 879 492 461 428 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 7338 7338
Size: 7634
BsaAI yAC'GTr
Cuts at: 4987 4987
Size: 7634
BsaBI GATnn'nnATC
Cuts at: 6 907 4222 5148 7185 6
Size: 901 3315 926 2037 455
Fragments arranged by size:
3315 2037 926 901 455
BsaHI Gr'CG_yC
Cuts at: 5065 5313 6015 7511 5065
Size: 248 702 1496 5188
Fragments arranged by size:
5188 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4823 5344 6022 6296 6443 6854 4823
Size: 521 678 274 147 411 5603
Fragments arranged by size:
5603 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 836 866 982 1012 2845 2875 2908 2938 3974
Size: 30 116 30 1833 30 33 30 1036
Cuts at: 3974 4004 836
Size: 30 4466
Fragments arranged by size:
4466 1833 1036 116 33 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 857 2560 4507 857
Size: 1703 1947 3984
Fragments arranged by size:
3984 1947 1703
BseYI C'CCAG_C
Cuts at: 930 2453 4381 5059 5817 6394 6890 930
Size: 1523 1928 678 758 577 496 1674
Fragments arranged by size:
1928 1674 1523 758 678 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 1270 4579 185
Size: 1085 3309 3240
Fragments arranged by size:
3309 3240 1085
BsiEI CG_ry'CG
Cuts at: 104 5176 5222 6430 104
Size: 5072 46 1208 1308
Fragments arranged by size:
5072 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 447 857 1706 3193 3614 4648 5426 5616 6408
Size: 410 849 1487 421 1034 778 190 792
Cuts at: 6408 447
Size: 1673
Fragments arranged by size:
1673 1487 1034 849 792 778 421 410
190
BsmI GAATG_Cn'
Cuts at: 635 2221 2280 2332 635
Size: 1586 59 52 5937
Fragments arranged by size:
5937 1586 59 52
BsmBI CGTCTCn'nnnn_
Cuts at: 2163 2611 4367 2163
Size: 448 1756 5430
Fragments arranged by size:
5430 1756 448
Bsp1286I G_dGCh'C
Cuts at: 447 857 1706 2810 2897 3193 3361 3614 3648
Size: 410 849 1104 87 296 168 253 34
Cuts at: 3648 4648 5259 5352 5426 5616 5678 6408 447
Size: 1000 611 93 74 190 62 730 1673
Fragments arranged by size:
1673 1104 1000 849 730 611 410 296
253 190 168 93 87 74 62 34
BspEI T'CCGG_A
Cuts at: 6854 6854
Size: 7634
BspHI T'CATG_A
Cuts at: 6810 6810
Size: 7634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3715 4684 4898 5200 5581 3715
Size: 969 214 302 381 5768
Fragments arranged by size:
5768 969 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5926 5980 7464 7629 50
Size: 5876 54 1484 165 55
Fragments arranged by size:
5876 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 586 1523 2336 2687 4181 5546 586
Size: 937 813 351 1494 1365 2674
Fragments arranged by size:
2674 1494 1365 937 813 351
BsrFI r'CCGG_y
Cuts at: 5632 5813 6022 5632
Size: 181 209 7244
Fragments arranged by size:
7244 209 181
BssSI C'ACGA_G
Cuts at: 5905 6263 5905
Size: 358 7276
BstAPI GCAn_nnn'nTGC
Cuts at: 1375 2338 2350 5011 1375
Size: 963 12 2661 3998
Fragments arranged by size:
3998 2661 963 12
BstBI TT'CG_AA
Cuts at: 5995 5995
Size: 7634
BstEII G'GTnAC_C
Cuts at: 1266 2436 4533 1266
Size: 1170 2097 4367
Fragments arranged by size:
4367 2097 1170
BstXI CCAn_nnnn'nTGG
Cuts at: 786 1548 4350 4355 786
Size: 762 2802 5 4065
Fragments arranged by size:
4065 2802 762 5
BstYI r'GATC_y
Cuts at: 1 708 1462 2457 2640 4659 5126 5149 5484
Size: 707 754 995 183 2019 467 23 335
Cuts at: 5484 5730 6742 6828 6840 7186 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2019 1012 995 754 707 467 449 346
335 246 183 86 23 12
BtgI C'CryG_G
Cuts at: 1064 2093 3048 4298 1064
Size: 1029 955 1250 4400
Fragments arranged by size:
4400 1250 1029 955
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5584 5765 5928 5584
Size: 181 163 7290
Fragments arranged by size:
7290 181 163
BtsI GCAGTG_nn'
Cuts at: 1832 3099 3133 4877 1832
Size: 1267 34 1744 4589
Fragments arranged by size:
4589 1744 1267 34
ClaI AT'CG_AT
Cuts at: 1834 1834
Size: 7634
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3473 3508 3473
Size: 35 7599
DraI TTT'AAA
Cuts at: 2783 4633 2783
Size: 1850 5784
DraIII CAC_nnn'GTG
Cuts at: 1906 3618 1906
Size: 1712 5922
DrdI GACnn_nn'nnGTC
Cuts at: 2059 5340 6198 2059
Size: 3281 858 3495
Fragments arranged by size:
3495 3281 858
EaeI y'GGCC_r
Cuts at: 312 416 1067 1656 2626 3962 4240 4357 5219
Size: 104 651 589 970 1336 278 117 862
Cuts at: 5219 5393 5784 5811 7156 312
Size: 174 391 27 1345 790
Fragments arranged by size:
1345 1336 970 862 790 651 589 391
278 174 117 104 27
EagI C'GGCC_G
Cuts at: 5219 5219
Size: 7634
EarI CTCTTCn'nnn_
Cuts at: 3221 3581 4395 5657 5867 3221
Size: 360 814 1262 210 4988
Fragments arranged by size:
4988 1262 814 360 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 814 1881 5842 6152 6298 7444 814
Size: 1067 3961 310 146 1146 1004
Fragments arranged by size:
3961 1146 1067 1004 310 146
EcoICRI GAG'CTC
Cuts at: 445 4646 445
Size: 4201 3433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 869 1463 1721 1870 3445 4145 4571 5459
Size: 679 594 258 149 1575 700 426 888
Cuts at: 5459 5891 6638 6906 190
Size: 432 747 268 918
Fragments arranged by size:
1575 918 888 747 700 679 594 432
426 268 258 149
EcoNI CCTnn'n_nnAGG
Cuts at: 3586 3586
Size: 7634
EcoO109I rG'GnC_Cy
Cuts at: 3644 4773 3644
Size: 1129 6505
EcoRI G'AATT_C
Cuts at: 4638 4638
Size: 7634
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5653 5653
Size: 7634
FspI TGC'GCA
Cuts at: 5415 5415
Size: 7634
HaeII r_GCGC'y
Cuts at: 5068 5316 6338 7317 5068
Size: 248 1022 979 5385
Fragments arranged by size:
5385 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 218 250 392 424 2698 2730 5476 5506 5508
Size: 32 142 32 2274 32 2746 30 2
Cuts at: 5508 5538 218
Size: 30 2314
Fragments arranged by size:
2746 2314 2274 142 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1197 3142 3948 4673 1197
Size: 1945 806 725 4158
Fragments arranged by size:
4158 1945 806 725
HindIII A'AGCT_T
Cuts at: 2561 2561
Size: 7634
KasI G'GCGC_C
Cuts at: 5064 5312 5064
Size: 248 7386
KpnI G_GTAC'C
Cuts at: 369 662 4654 369
Size: 293 3992 3349
Fragments arranged by size:
3992 3349 293
MfeI C'AATT_G
Cuts at: 2055 2055
Size: 7634
MluI A'CGCG_T
Cuts at: 2674 7067 2674
Size: 4393 3241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1993 3276 4033 6305 6489 1993
Size: 1283 757 2272 184 3138
Fragments arranged by size:
3138 2272 1283 757 184
MscI TGG'CCA
Cuts at: 314 418 1069 1658 2628 3964 5395 314
Size: 104 651 589 970 1336 1431 2553
Fragments arranged by size:
2553 1431 1336 970 651 589 104
MslI CAynn'nnrTG
Cuts at: 628 784 886 1142 2108 2269 3751 3958 3970
Size: 156 102 256 966 161 1482 207 12
Cuts at: 3970 4348 5623 5750 628
Size: 378 1275 127 2512
Fragments arranged by size:
2512 1482 1275 966 378 256 207 161
156 127 102 12
MspA1I CmG'CkG
Cuts at: 1773 2453 3409 4495 4745 5059 5419 6432 6677
Size: 680 956 1086 250 314 360 1013 245
Cuts at: 6677 1773
Size: 2730
Fragments arranged by size:
2730 1086 1013 956 680 360 314 250
245
NaeI GCC'GGC
Cuts at: 5815 5815
Size: 7634
NarI GG'CG_CC
Cuts at: 5065 5313 5065
Size: 248 7386
NcoI C'CATG_G
Cuts at: 1064 2093 3048 1064
Size: 1029 955 5650
Fragments arranged by size:
5650 1029 955
NgoMIV G'CCGG_C
Cuts at: 5813 5813
Size: 7634
NsiI A_TGCA'T
Cuts at: 1226 1226
Size: 7634
NspI r_CATG'y
Cuts at: 2715 3275 3715 4687 5187 6094 2715
Size: 560 440 972 500 907 4255
Fragments arranged by size:
4255 972 907 560 500 440
PciI A'CATG_T
Cuts at: 2711 3711 6090 2711
Size: 1000 2379 4255
Fragments arranged by size:
4255 2379 1000
PflMI CCAn_nnn'nTGG
Cuts at: 558 1000 1989 558
Size: 442 989 6203
Fragments arranged by size:
6203 989 442
PfoI T'CCnGG_A
Cuts at: 275 2392 275
Size: 2117 5517
PmeI GTTT'AAAC
Cuts at: 4633 4633
Size: 7634
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2845 2877 6773 6805 2845
Size: 32 3896 32 3674
Fragments arranged by size:
3896 3674 32 32
PspOMI G'GGCC_C
Cuts at: 3644 3644
Size: 7634
PstI C_TGCA'G
Cuts at: 2976 3374 4681 5366 2976
Size: 398 1307 685 5244
Fragments arranged by size:
5244 1307 685 398
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7634
PvuII CAG'CTG
Cuts at: 1773 3409 4495 5059 5419 1773
Size: 1636 1086 564 360 3988
Fragments arranged by size:
3988 1636 1086 564 360
RsrII CG'GwC_CG
Cuts at: 5829 5829
Size: 7634
SacI G_AGCT'C
Cuts at: 447 4648 447
Size: 4201 3433
SalI G'TCGA_C
Cuts at: 4671 4671
Size: 7634
SapI GCTCTTCn'nnn_
Cuts at: 5657 5867 5657
Size: 210 7424
SbfI CC_TGCA'GG
Cuts at: 4681 4681
Size: 7634
ScaI AGT'ACT
Cuts at: 7151 7151
Size: 7634
SfcI C'TryA_G
Cuts at: 33 586 794 2585 2972 3370 4599 4677 5362
Size: 553 208 1791 387 398 1229 78 685
Cuts at: 5362 6355 6546 33
Size: 993 191 1121
Fragments arranged by size:
1791 1229 1121 993 685 553 398 387
208 191 78
SfoI GGC'GCC
Cuts at: 5066 5314 5066
Size: 248 7386
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7634
SmaI CCC'GGG
Cuts at: 1274 4656 1274
Size: 3382 4252
SmlI C'TyrA_G
Cuts at: 1466 1705 3900 4364 4789 5704 6196 6458 1466
Size: 239 2195 464 425 915 492 262 2642
Fragments arranged by size:
2642 2195 915 492 464 425 262 239
SpeI A'CTAG_T
Cuts at: 67 1371 67
Size: 1304 6330
SphI G_CATG'C
Cuts at: 4687 5187 4687
Size: 500 7134
SspI AAT'ATT
Cuts at: 487 487
Size: 7634
StuI AGG'CCT
Cuts at: 1966 3516 1966
Size: 1550 6084
StyI C'CwwG_G
Cuts at: 746 1064 1148 2093 2355 2693 2889 2944 3048
Size: 318 84 945 262 338 196 55 104
Cuts at: 3048 3236 3442 3777 3968 4251 4768 5122 746
Size: 188 206 335 191 283 517 354 3258
Fragments arranged by size:
3258 945 517 354 338 335 318 283
262 206 196 191 188 104 84 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1274 1642 1894 2086 2200 2594 3629 5217 5350
Size: 368 252 192 114 394 1035 1588 133
Cuts at: 5350 5809 6018 1274
Size: 459 209 2890
Fragments arranged by size:
2890 1588 1035 459 394 368 252 209
192 133 114
TatI w'GTAC_w
Cuts at: 1009 3218 4607 7149 1009
Size: 2209 1389 2542 1494
Fragments arranged by size:
2542 2209 1494 1389
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 257 289 836 868 2430 2462 3044 3076 7151
Size: 32 547 32 1562 32 582 32 4075
Cuts at: 7151 7183 257
Size: 32 708
Fragments arranged by size:
4075 1562 708 582 547 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5431 5431
Size: 7634
XbaI T'CTAG_A
Cuts at: 4665 4665
Size: 7634
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1986 4128 5129 1986
Size: 2142 1001 4491
Fragments arranged by size:
4491 2142 1001
XmaI C'CCGG_G
Cuts at: 1272 4654 1272
Size: 3382 4252
XmnI GAAnn'nnTTC
Cuts at: 599 2036 3577 4151 599
Size: 1437 1541 574 4082
Fragments arranged by size:
4082 1541 1437 574
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI BaeI BmgBI BmtI BsiWI
BsrGI BssHII BstZ17I Bsu36I EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI PacI PasI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI