Restriction map for
pF1KB3033
(Circular) (Six-base) MAPSORT of: pF1KB3033.seq Check: 516 from: 1 to: 4802
pF1KB3033 4802 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 987 987
Size: 4802
AatII G_ACGT'C
Cuts at: 334 1574 1735 334
Size: 1240 161 3401
Fragments arranged by size:
3401 1240 161
AccI GT'mk_AC
Cuts at: 842 1478 1840 842
Size: 636 362 3804
Fragments arranged by size:
3804 636 362
Acc65I G'GTAC_C
Cuts at: 1818 1818
Size: 4802
AclI AA'CG_TT
Cuts at: 4642 4642
Size: 4802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 298 481 514 2627 3059 3806 4074 217
Size: 81 183 33 2113 432 747 268 945
Fragments arranged by size:
2113 945 747 432 268 183 81 33
AflIII A'CryG_T
Cuts at: 1784 2154 3258 4235 1784
Size: 370 1104 977 2351
Fragments arranged by size:
2351 1104 977 370
AgeI A'CCGG_T
Cuts at: 1016 3190 1016
Size: 2174 2628
AleI CACnn'nnGTG
Cuts at: 944 1591 944
Size: 647 4155
AlwNI CAG_nnn'CTG
Cuts at: 1011 2107 3674 1011
Size: 1096 1567 2139
Fragments arranged by size:
2139 1567 1096
ApaI G_GGCC'C
Cuts at: 483 908 1677 1711 483
Size: 425 769 34 3574
Fragments arranged by size:
3574 769 425 34
ApaLI G'TGCA_C
Cuts at: 1075 3572 1075
Size: 2497 2305
ApoI r'AATT_y
Cuts at: 1806 4155 4411 1806
Size: 2349 256 2197
Fragments arranged by size:
2349 2197 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4802
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4802
AvaI C'yCGr_G
Cuts at: 675 1676 1822 675
Size: 1001 146 3655
Fragments arranged by size:
3655 1001 146
BamHI G'GATC_C
Cuts at: 1827 1827
Size: 4802
BanI G'GyrC_C
Cuts at: 120 771 1818 2232 2480 2515 120
Size: 651 1047 414 248 35 2407
Fragments arranged by size:
2407 1047 651 414 248 35
BanII G_rGCy'C
Cuts at: 215 483 908 1400 1433 1677 1711 1816 2846
Size: 268 425 492 33 244 34 105 1030
Cuts at: 2846 215
Size: 2171
Fragments arranged by size:
2171 1030 492 425 268 244 105 34
33
BbeI G_GCGC'C
Cuts at: 124 2236 2484 124
Size: 2112 248 2442
Fragments arranged by size:
2442 2112 248
BbvCI CC'TCA_GC
Cuts at: 1597 1597
Size: 4802
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4768
BciVI GTATCCnnnnn_n'
Cuts at: 2700 3461 4802 2700
Size: 761 1341 2700
Fragments arranged by size:
2700 1341 761
BclI T'GATC_A
Cuts at: 2322 2322
Size: 4802
BglI GCCn_nnn'nGGC
Cuts at: 427 826 427
Size: 399 4403
BglII A'GATC_T
Cuts at: 485 1448 2317 4354 485
Size: 963 869 2037 933
Fragments arranged by size:
2037 963 933 869
BlpI GC'TnA_GC
Cuts at: 1122 1914 1122
Size: 792 4010
Bme1580I G_kGCm'C
Cuts at: 483 674 774 908 974 1079 1677 1711 2427
Size: 191 100 134 66 105 598 34 716
Cuts at: 2427 2520 3576 483
Size: 93 1056 1709
Fragments arranged by size:
1709 1056 716 598 191 134 105 100
93 66 34
BmgBI CAC'GTC
Cuts at: 339 1092 339
Size: 753 4049
BmrI ACTGGGnnnn_n'
Cuts at: 803 2014 2095 2430 4574 803
Size: 1211 81 335 2144 1031
Fragments arranged by size:
2144 1211 1031 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3169 3169
Size: 4802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 727 1064 1234 1339 1797 667
Size: 60 337 170 105 458 3672
Fragments arranged by size:
3672 458 337 170 105 60
Bpu10I CC'TnA_GC
Cuts at: 242 1597 242
Size: 1355 3447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1978 2857 3349 3647 1978
Size: 879 492 298 3133
Fragments arranged by size:
3133 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4506 4506
Size: 4802
BsaAI yAC'GTr
Cuts at: 1173 2155 1173
Size: 982 3820
BsaBI GATnn'nnATC
Cuts at: 6 2316 4353 6
Size: 2310 2037 455
Fragments arranged by size:
2310 2037 455
BsaHI Gr'CG_yC
Cuts at: 121 331 1571 1732 2233 2481 3183 4679 121
Size: 210 1240 161 501 248 702 1496 244
Fragments arranged by size:
1496 1240 702 501 248 244 210 161
BsaWI w'CCGG_w
Cuts at: 617 1016 1991 2512 3190 3464 3611 4022 617
Size: 399 975 521 678 274 147 411 1397
Fragments arranged by size:
1397 975 678 521 411 399 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 175 205 1315 1345 175
Size: 30 1110 30 3632
Fragments arranged by size:
3632 1110 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 180 334 1231 1323 1459 180
Size: 154 897 92 136 3523
Fragments arranged by size:
3523 897 154 136 92
BseYI C'CCAG_C
Cuts at: 1339 2227 2985 3562 4058 1339
Size: 888 758 577 496 2083
Fragments arranged by size:
2083 888 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 1597 361
Size: 1236 3566
BsiEI CG_ry'CG
Cuts at: 104 1088 2344 2390 3598 104
Size: 984 1256 46 1208 1308
Fragments arranged by size:
1308 1256 1208 984 46
BsiHKAI G_wGCw'C
Cuts at: 682 941 1079 1162 1223 1325 1816 2594 2784
Size: 259 138 83 61 102 491 778 190
Cuts at: 2784 3576 682
Size: 792 1908
Fragments arranged by size:
1908 792 778 491 259 190 138 102
83 61
BsmI GAATG_Cn'
Cuts at: 1608 1608
Size: 4802
BsmBI CGTCTCn'nnnn_
Cuts at: 1171 1495 1171
Size: 324 4478
Bsp1286I G_dGCh'C
Cuts at: 215 483 674 682 774 908 941 974 1079
Size: 268 191 8 92 134 33 33 105
Cuts at: 1079 1162 1223 1325 1400 1433 1677 1711 1816
Size: 83 61 102 75 33 244 34 105
Cuts at: 1816 2427 2520 2594 2784 2846 3576 215
Size: 611 93 74 190 62 730 1441
Fragments arranged by size:
1441 730 611 268 244 191 190 134
105 105 102 93 92 83 75 74
62 61 34 33 33 33 8
BspEI T'CCGG_A
Cuts at: 4022 4022
Size: 4802
BspHI T'CATG_A
Cuts at: 1436 3978 1436
Size: 2542 2260
BspMI ACCTGCnnnn'nnnn_
Cuts at: 987 1731 1785 1852 2066 2368 2749 987
Size: 744 54 67 214 302 381 3040
Fragments arranged by size:
3040 744 381 302 214 67 54
BsrBI CCG'CTC
Cuts at: 50 1082 1109 1121 3094 3148 4632 4797 50
Size: 1032 27 12 1973 54 1484 165 55
Fragments arranged by size:
1973 1484 1032 165 55 54 27 12
BsrDI GCAATG_nn'
Cuts at: 2714 2714
Size: 4802
BsrFI r'CCGG_y
Cuts at: 117 166 774 1016 1609 2800 2981 3190 117
Size: 49 608 242 593 1191 181 209 1729
Fragments arranged by size:
1729 1191 608 593 242 209 181 49
BssSI C'ACGA_G
Cuts at: 3073 3431 3073
Size: 358 4444
BstAPI GCAn_nnn'nTGC
Cuts at: 2179 2179
Size: 4802
BstBI TT'CG_AA
Cuts at: 3163 3163
Size: 4802
BstXI CCAn_nnnn'nTGG
Cuts at: 549 549
Size: 4802
BstYI r'GATC_y
Cuts at: 1 485 533 1448 1827 2294 2317 2652 2898
Size: 484 48 915 379 467 23 335 246
Cuts at: 2898 3910 3996 4008 4354 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 915 484 467 449 379 346 335
246 86 48 23 12
Bsu36I CC'TnA_GG
Cuts at: 1424 1424
Size: 4802
BtgI C'CryG_G
Cuts at: 366 548 1379 366
Size: 182 831 3789
Fragments arranged by size:
3789 831 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2752 2933 3096 2752
Size: 181 163 4458
Fragments arranged by size:
4458 181 163
BtsI GCAGTG_nn'
Cuts at: 743 815 972 1283 1556 2045 743
Size: 72 157 311 273 489 3500
Fragments arranged by size:
3500 489 311 273 157 72
DraI TTT'AAA
Cuts at: 1801 1801
Size: 4802
DraIII CAC_nnn'GTG
Cuts at: 1074 1074
Size: 4802
DrdI GACnn_nn'nnGTC
Cuts at: 2508 3366 2508
Size: 858 3944
EaeI y'GGCC_r
Cuts at: 203 380 722 827 1085 1140 1189 1355 2387
Size: 177 342 105 258 55 49 166 1032
Cuts at: 2387 2561 2952 2979 4324 203
Size: 174 391 27 1345 681
Fragments arranged by size:
1345 1032 681 391 342 258 177 174
166 105 55 49 27
EagI C'GGCC_G
Cuts at: 1085 2387 1085
Size: 1302 3500
EarI CTCTTCn'nnn_
Cuts at: 2825 3035 2825
Size: 210 4592
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 121 1070 3010 3320 3466 4612 121
Size: 949 1940 310 146 1146 311
Fragments arranged by size:
1940 1146 949 311 310 146
EcoICRI GAG'CTC
Cuts at: 1814 1814
Size: 4802
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 298 481 514 667 727 1064 1234 1339
Size: 81 183 33 153 60 337 170 105
Cuts at: 1339 1797 2627 3059 3806 4074 217
Size: 458 830 432 747 268 945
Fragments arranged by size:
945 830 747 458 432 337 268 183
170 153 105 81 60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 259 856 259
Size: 597 4205
EcoO109I rG'GnC_Cy
Cuts at: 273 456 905 1112 1262 1419 1673 1707 1941
Size: 183 449 207 150 157 254 34 234
Cuts at: 1941 273
Size: 3134
Fragments arranged by size:
3134 449 254 234 207 183 157 150
34
EcoRI G'AATT_C
Cuts at: 1806 1806
Size: 4802
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 4802
FseI GG_CCGG'CC
Cuts at: 170 170
Size: 4802
FspI TGC'GCA
Cuts at: 2583 2583
Size: 4802
HaeII r_GCGC'y
Cuts at: 124 2236 2484 3506 4485 124
Size: 2112 248 1022 979 441
Fragments arranged by size:
2112 1022 979 441 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1276 1308 2644 2674 2676 2706 1276
Size: 32 1336 30 2 30 3372
Fragments arranged by size:
3372 1336 32 30 30 2
HincII GTy'rAC
Cuts at: 1518 1841 1518
Size: 323 4479
KasI G'GCGC_C
Cuts at: 120 2232 2480 120
Size: 2112 248 2442
Fragments arranged by size:
2442 2112 248
KpnI G_GTAC'C
Cuts at: 1822 1822
Size: 4802
MluI A'CGCG_T
Cuts at: 1784 4235 1784
Size: 2451 2351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 1207 3473 3657 314
Size: 893 2266 184 1459
Fragments arranged by size:
2266 1459 893 184
MscI TGG'CCA
Cuts at: 205 382 829 1142 2563 205
Size: 177 447 313 1421 2444
Fragments arranged by size:
2444 1421 447 313 177
MslI CAynn'nnrTG
Cuts at: 667 820 944 1591 2791 2918 667
Size: 153 124 647 1200 127 2551
Fragments arranged by size:
2551 1200 647 153 127 124
MspA1I CmG'CkG
Cuts at: 598 898 1512 1913 2227 2587 3600 3845 598
Size: 300 614 401 314 360 1013 245 1555
Fragments arranged by size:
1555 1013 614 401 360 314 300 245
NaeI GCC'GGC
Cuts at: 119 168 1611 2983 119
Size: 49 1443 1372 1938
Fragments arranged by size:
1938 1443 1372 49
NarI GG'CG_CC
Cuts at: 121 2233 2481 121
Size: 2112 248 2442
Fragments arranged by size:
2442 2112 248
NcoI C'CATG_G
Cuts at: 366 548 366
Size: 182 4620
NgoMIV G'CCGG_C
Cuts at: 117 166 1609 2981 117
Size: 49 1443 1372 1938
Fragments arranged by size:
1938 1443 1372 49
NspI r_CATG'y
Cuts at: 510 1794 1855 2355 3262 510
Size: 1284 61 500 907 2050
Fragments arranged by size:
2050 1284 907 500 61
PasI CC'CwG_GG
Cuts at: 1767 1767
Size: 4802
PciI A'CATG_T
Cuts at: 3258 3258
Size: 4802
PflMI CCAn_nnn'nTGG
Cuts at: 901 1011 901
Size: 110 4692
PmeI GTTT'AAAC
Cuts at: 1801 1801
Size: 4802
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 3941
Size: 32 4770
PpuMI rG'GwC_Cy
Cuts at: 273 456 1112 1262 1419 273
Size: 183 656 150 157 3656
Fragments arranged by size:
3656 656 183 157 150
PspOMI G'GGCC_C
Cuts at: 479 904 1673 1707 479
Size: 425 769 34 3574
Fragments arranged by size:
3574 769 425 34
PstI C_TGCA'G
Cuts at: 597 1251 1278 1511 1849 2534 597
Size: 654 27 233 338 685 2865
Fragments arranged by size:
2865 685 654 338 233 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4802
PvuII CAG'CTG
Cuts at: 898 1512 2227 2587 898
Size: 614 715 360 3113
Fragments arranged by size:
3113 715 614 360
RsrII CG'GwC_CG
Cuts at: 2997 2997
Size: 4802
SacI G_AGCT'C
Cuts at: 1816 1816
Size: 4802
SalI G'TCGA_C
Cuts at: 1839 1839
Size: 4802
SanDI GG'GwC_CC
Cuts at: 1112 1112
Size: 4802
SapI GCTCTTCn'nnn_
Cuts at: 2825 3035 2825
Size: 210 4592
SbfI CC_TGCA'GG
Cuts at: 1849 1849
Size: 4802
ScaI AGT'ACT
Cuts at: 4319 4319
Size: 4802
SfcI C'TryA_G
Cuts at: 33 593 1247 1274 1507 1845 2530 3523 3714
Size: 560 654 27 233 338 685 993 191
Cuts at: 3714 33
Size: 1121
Fragments arranged by size:
1121 993 685 654 560 338 233 191
27
SfoI GGC'GCC
Cuts at: 122 2234 2482 122
Size: 2112 248 2442
Fragments arranged by size:
2442 2112 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4802
SmaI CCC'GGG
Cuts at: 1824 1824
Size: 4802
SmlI C'TyrA_G
Cuts at: 1957 2872 3364 3626 1957
Size: 915 492 262 3133
Fragments arranged by size:
3133 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4802
SphI G_CATG'C
Cuts at: 1855 2355 1855
Size: 500 4302
StuI AGG'CCT
Cuts at: 265 265
Size: 4802
StyI C'CwwG_G
Cuts at: 366 548 1936 2290 366
Size: 182 1388 354 2878
Fragments arranged by size:
2878 1388 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1353 2385 2518 2977 3186 1353
Size: 1032 133 459 209 2969
Fragments arranged by size:
2969 1032 459 209 133
TatI w'GTAC_w
Cuts at: 1461 1493 4317 1461
Size: 32 2824 1946
Fragments arranged by size:
2824 1946 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 175 207 362 394 795 827 1315 1347 1703
Size: 32 155 32 401 32 488 32 356
Cuts at: 1703 1735 4319 4351 175
Size: 32 2584 32 626
Fragments arranged by size:
2584 626 488 401 356 155 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 922 2599 922
Size: 1677 3125
XbaI T'CTAG_A
Cuts at: 1833 1833
Size: 4802
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 303 549 636 1663 2297 303
Size: 246 87 1027 634 2808
Fragments arranged by size:
2808 1027 634 246 87
XmaI C'CCGG_G
Cuts at: 1822 1822
Size: 4802
ZraI GAC'GTC
Cuts at: 332 1572 1733 332
Size: 1240 161 3401
Fragments arranged by size:
3401 1240 161
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
ClaI CspCI EcoRV FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI