Restriction map for
pF1KB3031
(Circular) (Six-base) MAPSORT of: pF1KB3031.seq Check: 1822 from: 1 to: 4892
pF1KB3031 4892 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1930 1930
Size: 4892
Acc65I G'GTAC_C
Cuts at: 1908 1908
Size: 4892
AclI AA'CG_TT
Cuts at: 4732 4732
Size: 4892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 3149 3502 4164 2717
Size: 432 353 662 3445
Fragments arranged by size:
3445 662 432 353
AflIII A'CryG_T
Cuts at: 2244 4044 4325 2244
Size: 1800 281 2811
Fragments arranged by size:
2811 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 597 597
Size: 4892
AleI CACnn'nnGTG
Cuts at: 145 989 1484 145
Size: 844 495 3553
Fragments arranged by size:
3553 844 495
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1502 1502
Size: 4892
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 533 565 533
Size: 32 4860
AlwNI CAG_nnn'CTG
Cuts at: 636 2197 3635 636
Size: 1561 1438 1893
Fragments arranged by size:
1893 1561 1438
ApaLI G'TGCA_C
Cuts at: 1432 1477 3730 1432
Size: 45 2253 2594
Fragments arranged by size:
2594 2253 45
ApoI r'AATT_y
Cuts at: 316 856 1045 1896 4245 4501 316
Size: 540 189 851 2349 256 707
Fragments arranged by size:
2349 851 707 540 256 189
AseI AT'TA_AT
Cuts at: 20 20
Size: 4892
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
AvaI C'yCGr_G
Cuts at: 1157 1912 1157
Size: 755 4137
BamHI G'GATC_C
Cuts at: 1917 1917
Size: 4892
BanI G'GyrC_C
Cuts at: 1908 2322 2570 2605 1908
Size: 414 248 35 4195
Fragments arranged by size:
4195 414 248 35
BanII G_rGCy'C
Cuts at: 1783 1906 2936 1783
Size: 123 1030 3739
Fragments arranged by size:
3739 1030 123
BbeI G_GCGC'C
Cuts at: 2326 2574 2326
Size: 248 4644
BbsI GAAGACnn'nnnn_
Cuts at: 1835 1835
Size: 4892
BciVI GTATCCnnnnn_n'
Cuts at: 2790 3846 4892 2790
Size: 1056 1046 2790
Fragments arranged by size:
2790 1056 1046
BclI T'GATC_A
Cuts at: 963 2412 963
Size: 1449 3443
BglI GCCn_nnn'nGGC
Cuts at: 328 328
Size: 4892
BglII A'GATC_T
Cuts at: 681 2407 4444 681
Size: 1726 2037 1129
Fragments arranged by size:
2037 1726 1129
BlpI GC'TnA_GC
Cuts at: 2004 2004
Size: 4892
Bme1580I G_kGCm'C
Cuts at: 1394 1436 1481 1727 2517 2610 3734 1394
Size: 42 45 246 790 93 1124 2552
Fragments arranged by size:
2552 1124 790 246 93 45 42
BmrI ACTGGGnnnn_n'
Cuts at: 2104 2185 2520 4664 2104
Size: 81 335 2144 2332
Fragments arranged by size:
2332 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3259 3259
Size: 4892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 560 1444 242
Size: 318 884 3690
Fragments arranged by size:
3690 884 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 2068 2947 3661 3959 727
Size: 1341 879 714 298 1660
Fragments arranged by size:
1660 1341 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 814 1607 4596 814
Size: 793 2989 1110
Fragments arranged by size:
2989 1110 793
BsaAI yAC'GTr
Cuts at: 2245 2245
Size: 4892
BsaBI GATnn'nnATC
Cuts at: 6 992 2406 4443 6
Size: 986 1414 2037 455
Fragments arranged by size:
2037 1414 986 455
BsaHI Gr'CG_yC
Cuts at: 1250 2323 2571 3273 4769 1250
Size: 1073 248 702 1496 1373
Fragments arranged by size:
1496 1373 1073 702 248
BsaWI w'CCGG_w
Cuts at: 2081 2602 3280 3691 3838 4112 2081
Size: 521 678 411 147 274 2861
Fragments arranged by size:
2861 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 533 563 533
Size: 30 4862
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 191 671 1441 191
Size: 480 770 3642
Fragments arranged by size:
3642 770 480
BseYI C'CCAG_C
Cuts at: 1393 2317 3075 3740 4148 1393
Size: 924 758 665 408 2137
Fragments arranged by size:
2137 924 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 1835 440
Size: 1395 3497
BsiEI CG_ry'CG
Cuts at: 104 1472 2434 2480 3710 104
Size: 1368 962 46 1230 1286
Fragments arranged by size:
1368 1286 1230 962 46
BsiHKAI G_wGCw'C
Cuts at: 1436 1481 1906 2684 2874 3734 1436
Size: 45 425 778 190 860 2594
Fragments arranged by size:
2594 860 778 425 190 45
Bsp1286I G_dGCh'C
Cuts at: 1394 1436 1481 1727 1783 1906 2517 2610 2684
Size: 42 45 246 56 123 611 93 74
Cuts at: 2684 2874 2936 3734 1394
Size: 190 62 798 2552
Fragments arranged by size:
2552 798 611 246 190 123 93 74
62 56 45 42
BspHI T'CATG_A
Cuts at: 1169 1287 3324 1169
Size: 118 2037 2737
Fragments arranged by size:
2737 2037 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1942 2156 2458 2839 1942
Size: 214 302 381 3995
Fragments arranged by size:
3995 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1285 3184 3238 4722 4887 50
Size: 1235 1899 54 1484 165 55
Fragments arranged by size:
1899 1484 1235 165 55 54
BsrDI GCAATG_nn'
Cuts at: 594 1088 2804 594
Size: 494 1716 2682
Fragments arranged by size:
2682 1716 494
BsrFI r'CCGG_y
Cuts at: 1467 2890 3071 1467
Size: 1423 181 3288
Fragments arranged by size:
3288 1423 181
BssSI C'ACGA_G
Cuts at: 3163 3871 3163
Size: 708 4184
BstAPI GCAn_nnn'nTGC
Cuts at: 2269 2269
Size: 4892
BstBI TT'CG_AA
Cuts at: 3253 3253
Size: 4892
BstXI CCAn_nnnn'nTGG
Cuts at: 1593 1768 1593
Size: 175 4717
BstYI r'GATC_y
Cuts at: 1 423 681 1917 2384 2407 2742 2988 3294
Size: 422 258 1236 467 23 335 246 306
Cuts at: 3294 3306 3392 4444 1
Size: 12 86 1052 449
Fragments arranged by size:
1236 1052 467 449 422 335 306 258
246 86 23 12
BtgI C'CryG_G
Cuts at: 1383 1383
Size: 4892
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 98 2842 3023 3186 98
Size: 2744 181 163 1804
Fragments arranged by size:
2744 1804 181 163
BtsI GCAGTG_nn'
Cuts at: 1479 1491 2135 1479
Size: 12 644 4236
Fragments arranged by size:
4236 644 12
DraI TTT'AAA
Cuts at: 1891 1891
Size: 4892
DraIII CAC_nnn'GTG
Cuts at: 1389 1813 1389
Size: 424 4468
DrdI GACnn_nn'nnGTC
Cuts at: 2598 3942 2598
Size: 1344 3548
EaeI y'GGCC_r
Cuts at: 1375 1497 2477 2651 3042 3069 4414 1375
Size: 122 980 174 391 27 1345 1853
Fragments arranged by size:
1853 1345 980 391 174 122 27
EagI C'GGCC_G
Cuts at: 2477 2477
Size: 4892
EarI CTCTTCn'nnn_
Cuts at: 1694 2915 3125 1694
Size: 1221 210 3461
Fragments arranged by size:
3461 1221 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1149 3100 3842 3988 4702 1149
Size: 1951 742 146 714 1339
Fragments arranged by size:
1951 1339 742 714 146
EcoICRI GAG'CTC
Cuts at: 1904 1904
Size: 4892
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 560 1444 2717 3149 3502 4164 242
Size: 318 884 1273 432 353 662 970
Fragments arranged by size:
1273 970 884 662 432 353 318
EcoNI CCTnn'n_nnAGG
Cuts at: 435 1426 435
Size: 991 3901
EcoO109I rG'GnC_Cy
Cuts at: 2031 2031
Size: 4892
EcoRI G'AATT_C
Cuts at: 1896 1896
Size: 4892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 4892
FspI TGC'GCA
Cuts at: 562 2673 562
Size: 2111 2781
FspAI rTGC'GCAy
Cuts at: 562 562
Size: 4892
HaeII r_GCGC'y
Cuts at: 1283 2326 2574 3804 4575 1283
Size: 1043 248 1230 771 1600
Fragments arranged by size:
1600 1230 1043 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 415 447 583 612 615 644 1151 1183 1758
Size: 32 136 29 3 29 507 32 575
Cuts at: 1758 1790 2734 2764 2766 2796 415
Size: 32 944 30 2 30 2511
Fragments arranged by size:
2511 944 575 507 136 32 32 32
30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 170 1560 1931 170
Size: 1390 371 3131
Fragments arranged by size:
3131 1390 371
KasI G'GCGC_C
Cuts at: 2322 2570 2322
Size: 248 4644
KpnI G_GTAC'C
Cuts at: 1912 1912
Size: 4892
MluI A'CGCG_T
Cuts at: 4325 4325
Size: 4892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1825 3651 3835 1305
Size: 520 1826 184 2362
Fragments arranged by size:
2362 1826 520 184
MscI TGG'CCA
Cuts at: 2653 2653
Size: 4892
MslI CAynn'nnrTG
Cuts at: 145 989 1484 1591 2881 3008 145
Size: 844 495 107 1290 127 2029
Fragments arranged by size:
2029 1290 844 495 127 107
MspA1I CmG'CkG
Cuts at: 2003 2317 2677 3461 3706 2003
Size: 314 360 784 245 3189
Fragments arranged by size:
3189 784 360 314 245
NaeI GCC'GGC
Cuts at: 3073 3073
Size: 4892
NarI GG'CG_CC
Cuts at: 2323 2571 2323
Size: 248 4644
NgoMIV G'CCGG_C
Cuts at: 3071 3071
Size: 4892
NspI r_CATG'y
Cuts at: 1945 2445 4048 1945
Size: 500 1603 2789
Fragments arranged by size:
2789 1603 500
PasI CC'CwG_GG
Cuts at: 1402 1402
Size: 4892
PciI A'CATG_T
Cuts at: 4044 4044
Size: 4892
PfoI T'CCnGG_A
Cuts at: 1421 1421
Size: 4892
PmeI GTTT'AAAC
Cuts at: 1891 1891
Size: 4892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 533 565 3338 3370 533
Size: 32 2773 32 2055
Fragments arranged by size:
2773 2055 32 32
PsiI TTA'TAA
Cuts at: 698 698
Size: 4892
PstI C_TGCA'G
Cuts at: 1486 1939 2624 1486
Size: 453 685 3754
Fragments arranged by size:
3754 685 453
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4892
PvuII CAG'CTG
Cuts at: 2317 2677 2317
Size: 360 4532
RsrII CG'GwC_CG
Cuts at: 3087 3087
Size: 4892
SacI G_AGCT'C
Cuts at: 1906 1906
Size: 4892
SalI G'TCGA_C
Cuts at: 1929 1929
Size: 4892
SapI GCTCTTCn'nnn_
Cuts at: 1694 2915 3125 1694
Size: 1221 210 3461
Fragments arranged by size:
3461 1221 210
SbfI CC_TGCA'GG
Cuts at: 1939 1939
Size: 4892
ScaI AGT'ACT
Cuts at: 734 4409 734
Size: 3675 1217
SfcI C'TryA_G
Cuts at: 33 894 1482 1681 1935 2620 3588 3779 33
Size: 861 588 199 254 685 968 191 1146
Fragments arranged by size:
1146 968 861 685 588 254 199 191
SfoI GGC'GCC
Cuts at: 2324 2572 2324
Size: 248 4644
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
SmaI CCC'GGG
Cuts at: 1914 1914
Size: 4892
SmlI C'TyrA_G
Cuts at: 742 2047 2962 3676 3938 742
Size: 1305 915 714 262 1696
Fragments arranged by size:
1696 1305 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4892
SphI G_CATG'C
Cuts at: 1945 2445 1945
Size: 500 4392
SspI AAT'ATT
Cuts at: 1740 1740
Size: 4892
StuI AGG'CCT
Cuts at: 204 204
Size: 4892
StyI C'CwwG_G
Cuts at: 2026 2380 2026
Size: 354 4538
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 146 277 2475 2608 3067 3276 146
Size: 131 2198 133 459 209 1762
Fragments arranged by size:
2198 1762 459 209 133 131
TatI w'GTAC_w
Cuts at: 732 1795 4407 732
Size: 1063 2612 1217
Fragments arranged by size:
2612 1217 1063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4409 4441 4409
Size: 32 4860
Tth111I GACn'n_nGTC
Cuts at: 2689 2689
Size: 4892
XbaI T'CTAG_A
Cuts at: 1923 1923
Size: 4892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 535 2387 535
Size: 1852 3040
XmaI C'CCGG_G
Cuts at: 1912 1912
Size: 4892
XmnI GAAnn'nnTTC
Cuts at: 1879 1879
Size: 4892
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI