Restriction map for
pF1KB3016
(Circular) (Six-base) MAPSORT of: pF1KB3016.seq Check: 4163 from: 1 to: 5450
pF1KB3016 5450 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 2327 2327
Size: 5450
AccI GT'mk_AC
Cuts at: 2144 2488 2144
Size: 344 5106
Acc65I G'GTAC_C
Cuts at: 2466 2466
Size: 5450
AclI AA'CG_TT
Cuts at: 2023 5290 2023
Size: 3267 2183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 1295 3275 3707 4060 4722 485
Size: 810 1980 432 353 662 1213
Fragments arranged by size:
1980 1213 810 662 432 353
AflIII A'CryG_T
Cuts at: 1594 2802 4602 4883 1594
Size: 1208 1800 281 2161
Fragments arranged by size:
2161 1800 1208 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 491 523 2039 2071 491
Size: 32 1516 32 3870
Fragments arranged by size:
3870 1516 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1000 2755 4193 1000
Size: 1755 1438 2257
Fragments arranged by size:
2257 1755 1438
ApaLI G'TGCA_C
Cuts at: 4288 4288
Size: 5450
ApoI r'AATT_y
Cuts at: 604 826 2209 2454 4803 5059 604
Size: 222 1383 245 2349 256 995
Fragments arranged by size:
2349 1383 995 256 245 222
AseI AT'TA_AT
Cuts at: 20 20
Size: 5450
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5450
AvaI C'yCGr_G
Cuts at: 2470 2470
Size: 5450
BamHI G'GATC_C
Cuts at: 487 1464 2475 487
Size: 977 1011 3462
Fragments arranged by size:
3462 1011 977
BanI G'GyrC_C
Cuts at: 138 1823 2466 2880 3128 3163 138
Size: 1685 643 414 248 35 2425
Fragments arranged by size:
2425 1685 643 414 248 35
BanII G_rGCy'C
Cuts at: 1812 2382 2464 3494 1812
Size: 570 82 1030 3768
Fragments arranged by size:
3768 1030 570 82
BbeI G_GCGC'C
Cuts at: 142 2884 3132 142
Size: 2742 248 2460
Fragments arranged by size:
2742 2460 248
BbsI GAAGACnn'nnnn_
Cuts at: 1668 1668
Size: 5450
BbvCI CC'TCA_GC
Cuts at: 2073 2073
Size: 5450
BciVI GTATCCnnnnn_n'
Cuts at: 493 3348 4404 5450 493
Size: 2855 1056 1046 493
Fragments arranged by size:
2855 1056 1046 493
BclI T'GATC_A
Cuts at: 2970 2970
Size: 5450
BglI GCCn_nnn'nGGC
Cuts at: 147 1298 147
Size: 1151 4299
BglII A'GATC_T
Cuts at: 1632 2008 2965 5002 1632
Size: 376 957 2037 2080
Fragments arranged by size:
2080 2037 957 376
BlpI GC'TnA_GC
Cuts at: 2562 2562
Size: 5450
Bme1580I G_kGCm'C
Cuts at: 3075 3168 4292 3075
Size: 93 1124 4233
Fragments arranged by size:
4233 1124 93
BmgBI CAC'GTC
Cuts at: 236 2245 236
Size: 2009 3441
BmrI ACTGGGnnnn_n'
Cuts at: 2047 2662 2743 3078 5222 2047
Size: 615 81 335 2144 2275
Fragments arranged by size:
2275 2144 615 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 210 3817 210
Size: 3607 1843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 944 1267 1769 1796 163
Size: 781 323 502 27 3817
Fragments arranged by size:
3817 781 502 323 27
Bpu10I CC'TnA_GC
Cuts at: 2073 2073
Size: 5450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2410 2626 3505 4219 4517 2410
Size: 216 879 714 298 3343
Fragments arranged by size:
3343 879 714 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 167 581 1927 5154 167
Size: 414 1346 3227 463
Fragments arranged by size:
3227 1346 463 414
BsaAI yAC'GTr
Cuts at: 2803 2803
Size: 5450
BsaBI GATnn'nnATC
Cuts at: 6 2964 5001 6
Size: 2958 2037 455
Fragments arranged by size:
2958 2037 455
BsaHI Gr'CG_yC
Cuts at: 139 2324 2881 3129 3831 5327 139
Size: 2185 557 248 702 1496 262
Fragments arranged by size:
2185 1496 702 557 262 248
BsaWI w'CCGG_w
Cuts at: 2639 3160 3838 4249 4396 4670 2639
Size: 521 678 411 147 274 3419
Fragments arranged by size:
3419 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 1104 169
Size: 935 4515
BseYI C'CCAG_C
Cuts at: 2875 3633 4298 4706 2875
Size: 758 665 408 3619
Fragments arranged by size:
3619 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1619 1619
Size: 5450
BsiEI CG_ry'CG
Cuts at: 104 2992 3038 4268 104
Size: 2888 46 1230 1286
Fragments arranged by size:
2888 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1812 2464 3242 3432 4292 1812
Size: 652 778 190 860 2970
Fragments arranged by size:
2970 860 778 652 190
BsmI GAATG_Cn'
Cuts at: 766 766
Size: 5450
Bsp1286I G_dGCh'C
Cuts at: 1812 2382 2464 3075 3168 3242 3432 3494 4292
Size: 570 82 611 93 74 190 62 798
Cuts at: 4292 1812
Size: 2970
Fragments arranged by size:
2970 798 611 570 190 93 82 74
62
BspHI T'CATG_A
Cuts at: 2082 2118 3882 2082
Size: 36 1764 3650
Fragments arranged by size:
3650 1764 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2500 2714 3016 3397 2500
Size: 214 302 381 4553
Fragments arranged by size:
4553 381 302 214
BsrBI CCG'CTC
Cuts at: 50 165 189 3742 3796 5280 5445 50
Size: 115 24 3553 54 1484 165 55
Fragments arranged by size:
3553 1484 165 115 55 54 24
BsrDI GCAATG_nn'
Cuts at: 1661 3362 1661
Size: 1701 3749
BsrFI r'CCGG_y
Cuts at: 3448 3629 3448
Size: 181 5269
BssSI C'ACGA_G
Cuts at: 848 3721 4429 848
Size: 2873 708 1869
Fragments arranged by size:
2873 1869 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2827 2827
Size: 5450
BstBI TT'CG_AA
Cuts at: 3811 3811
Size: 5450
BstEII G'GTnAC_C
Cuts at: 2130 2130
Size: 5450
BstXI CCAn_nnnn'nTGG
Cuts at: 1820 1820
Size: 5450
BstYI r'GATC_y
Cuts at: 1 487 1328 1464 1632 2008 2475 2942 2965
Size: 486 841 136 168 376 467 467 23
Cuts at: 2965 3300 3546 3852 3864 3950 5002 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 841 486 467 467 449 376 335
306 246 168 136 86 23 12
BtgI C'CryG_G
Cuts at: 896 896
Size: 5450
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 192 3400 3581 3744 192
Size: 3208 181 163 1898
Fragments arranged by size:
3208 1898 181 163
BtsI GCAGTG_nn'
Cuts at: 2238 2693 2238
Size: 455 4995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1785 1820 1785
Size: 35 5415
DraI TTT'AAA
Cuts at: 719 2449 719
Size: 1730 3720
DrdI GACnn_nn'nnGTC
Cuts at: 3156 4500 3156
Size: 1344 4106
EaeI y'GGCC_r
Cuts at: 948 1290 1536 3035 3209 3600 3627 4972 948
Size: 342 246 1499 174 391 27 1345 1426
Fragments arranged by size:
1499 1426 1345 391 342 246 174 27
EagI C'GGCC_G
Cuts at: 3035 3035
Size: 5450
EarI CTCTTCn'nnn_
Cuts at: 179 215 3473 3683 179
Size: 36 3258 210 1946
Fragments arranged by size:
3258 1946 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3658 4400 4546 5260 3658
Size: 742 146 714 3848
Fragments arranged by size:
3848 742 714 146
EcoICRI GAG'CTC
Cuts at: 1810 2462 1810
Size: 652 4798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 485 944 1267 1295 1769 1796 3275 3707
Size: 322 459 323 28 474 27 1479 432
Cuts at: 3707 4060 4722 163
Size: 353 662 891
Fragments arranged by size:
1479 891 662 474 459 432 353 323
322 28 27
EcoO109I rG'GnC_Cy
Cuts at: 1945 2408 2589 1945
Size: 463 181 4806
Fragments arranged by size:
4806 463 181
EcoRI G'AATT_C
Cuts at: 2454 2454
Size: 5450
EcoRV GAT'ATC
Cuts at: 657 903 657
Size: 246 5204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1900 3469 1900
Size: 1569 3881
FspI TGC'GCA
Cuts at: 3231 3231
Size: 5450
HaeII r_GCGC'y
Cuts at: 142 2884 3132 4362 5133 142
Size: 2742 248 1230 771 459
Fragments arranged by size:
2742 1230 771 459 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1065 1097 1880 1912 2299 2331 2344 2376 3292
Size: 32 783 32 387 32 13 32 916
Cuts at: 3292 3322 3324 3354 1065
Size: 30 2 30 3161
Fragments arranged by size:
3161 916 783 387 32 32 32 32
30 30 13 2
HincII GTy'rAC
Cuts at: 2489 2489
Size: 5450
HindIII A'AGCT_T
Cuts at: 1903 1903
Size: 5450
KasI G'GCGC_C
Cuts at: 138 2880 3128 138
Size: 2742 248 2460
Fragments arranged by size:
2742 2460 248
KpnI G_GTAC'C
Cuts at: 2470 2470
Size: 5450
MluI A'CGCG_T
Cuts at: 4883 4883
Size: 5450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 283 1217 4209 4393 283
Size: 934 2992 184 1340
Fragments arranged by size:
2992 1340 934 184
MscI TGG'CCA
Cuts at: 950 1538 3211 950
Size: 588 1673 3189
Fragments arranged by size:
3189 1673 588
MslI CAynn'nnrTG
Cuts at: 911 3439 3566 911
Size: 2528 127 2795
Fragments arranged by size:
2795 2528 127
MspA1I CmG'CkG
Cuts at: 2561 2875 3235 4019 4264 2561
Size: 314 360 784 245 3747
Fragments arranged by size:
3747 784 360 314 245
NaeI GCC'GGC
Cuts at: 3631 3631
Size: 5450
NarI GG'CG_CC
Cuts at: 139 2881 3129 139
Size: 2742 248 2460
Fragments arranged by size:
2742 2460 248
NcoI C'CATG_G
Cuts at: 896 896
Size: 5450
NgoMIV G'CCGG_C
Cuts at: 3629 3629
Size: 5450
NspI r_CATG'y
Cuts at: 1598 2503 3003 4606 1598
Size: 905 500 1603 2442
Fragments arranged by size:
2442 1603 905 500
PciI A'CATG_T
Cuts at: 1594 4602 1594
Size: 3008 2442
PflMI CCAn_nnn'nTGG
Cuts at: 1114 1114
Size: 5450
PfoI T'CCnGG_A
Cuts at: 179 1244 179
Size: 1065 4385
PmeI GTTT'AAAC
Cuts at: 2449 2449
Size: 5450
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 5418
PpuMI rG'GwC_Cy
Cuts at: 2408 2408
Size: 5450
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 633 665 633
Size: 32 5418
PstI C_TGCA'G
Cuts at: 2497 3182 2497
Size: 685 4765
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5450
PvuII CAG'CTG
Cuts at: 2875 3235 2875
Size: 360 5090
RsrII CG'GwC_CG
Cuts at: 3645 3645
Size: 5450
SacI G_AGCT'C
Cuts at: 1812 2464 1812
Size: 652 4798
SalI G'TCGA_C
Cuts at: 2487 2487
Size: 5450
SapI GCTCTTCn'nnn_
Cuts at: 179 3473 3683 179
Size: 3294 210 1946
Fragments arranged by size:
3294 1946 210
SbfI CC_TGCA'GG
Cuts at: 2497 2497
Size: 5450
ScaI AGT'ACT
Cuts at: 1961 4967 1961
Size: 3006 2444
SfcI C'TryA_G
Cuts at: 33 650 1332 1406 2493 3178 4146 4337 33
Size: 617 682 74 1087 685 968 191 1146
Fragments arranged by size:
1146 1087 968 685 682 617 191 74
SfoI GGC'GCC
Cuts at: 140 2882 3130 140
Size: 2742 248 2460
Fragments arranged by size:
2742 2460 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5450
SmaI CCC'GGG
Cuts at: 2472 2472
Size: 5450
SmlI C'TyrA_G
Cuts at: 2389 2605 3520 4234 4496 2389
Size: 216 915 714 262 3343
Fragments arranged by size:
3343 915 714 262 216
SpeI A'CTAG_T
Cuts at: 67 1393 67
Size: 1326 4124
SphI G_CATG'C
Cuts at: 2503 3003 2503
Size: 500 4950
SspI AAT'ATT
Cuts at: 788 1052 1146 788
Size: 264 94 5092
Fragments arranged by size:
5092 264 94
StyI C'CwwG_G
Cuts at: 142 896 2584 2938 142
Size: 754 1688 354 2654
Fragments arranged by size:
2654 1688 754 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3033 3166 3625 3834 3033
Size: 133 459 209 4649
Fragments arranged by size:
4649 459 209 133
TatI w'GTAC_w
Cuts at: 1959 4965 1959
Size: 3006 2444
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4967 4999 4967
Size: 32 5418
Tth111I GACn'n_nGTC
Cuts at: 3247 3247
Size: 5450
XbaI T'CTAG_A
Cuts at: 2481 2481
Size: 5450
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1233 2945 1233
Size: 1712 3738
XmaI C'CCGG_G
Cuts at: 2470 2470
Size: 5450
ZraI GAC'GTC
Cuts at: 2325 2325
Size: 5450
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BmtI BsaXI BsiWI
BsmBI BspEI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII
EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI