Restriction map for
pF1KB3009
(Circular) (Six-base) MAPSORT of: pF1KB3009.seq Check: 6193 from: 1 to: 5057
pF1KB3009 5057 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1197 2095 1197
Size: 898 4159
Acc65I G'GTAC_C
Cuts at: 1760 2073 1760
Size: 313 4744
AclI AA'CG_TT
Cuts at: 820 4897 820
Size: 4077 980
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 1644 1728 1896 1980 2882 3314 4061 4329
Size: 73 84 168 84 902 432 747 268
Cuts at: 4329 1571
Size: 2299
Fragments arranged by size:
2299 902 747 432 268 168 84 84
73
AflIII A'CryG_T
Cuts at: 2409 3513 4490 2409
Size: 1104 977 2976
Fragments arranged by size:
2976 1104 977
AgeI A'CCGG_T
Cuts at: 3445 3445
Size: 5057
AlwNI CAG_nnn'CTG
Cuts at: 205 330 708 813 2362 3929 205
Size: 125 378 105 1549 1567 1333
Fragments arranged by size:
1567 1549 1333 378 125 105
ApaLI G'TGCA_C
Cuts at: 3827 3827
Size: 5057
ApoI r'AATT_y
Cuts at: 158 1148 1488 2061 4410 4666 158
Size: 990 340 573 2349 256 549
Fragments arranged by size:
2349 990 573 549 340 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5057
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5057
AvaI C'yCGr_G
Cuts at: 619 733 2077 619
Size: 114 1344 3599
Fragments arranged by size:
3599 1344 114
BamHI G'GATC_C
Cuts at: 597 2082 597
Size: 1485 3572
BanI G'GyrC_C
Cuts at: 663 1433 1760 2073 2487 2735 2770 663
Size: 770 327 313 414 248 35 2950
Fragments arranged by size:
2950 770 414 327 313 248 35
BanII G_rGCy'C
Cuts at: 652 701 766 2071 3101 652
Size: 49 65 1305 1030 2608
Fragments arranged by size:
2608 1305 1030 65 49
BbeI G_GCGC'C
Cuts at: 2491 2739 2491
Size: 248 4809
BbsI GAAGACnn'nnnn_
Cuts at: 920 1783 920
Size: 863 4194
BbvCI CC'TCA_GC
Cuts at: 1790 1790
Size: 5057
BciVI GTATCCnnnnn_n'
Cuts at: 2955 3716 5057 2955
Size: 761 1341 2955
Fragments arranged by size:
2955 1341 761
BclI T'GATC_A
Cuts at: 2577 2577
Size: 5057
BglII A'GATC_T
Cuts at: 654 965 1101 2572 4609 654
Size: 311 136 1471 2037 1102
Fragments arranged by size:
2037 1471 1102 311 136
BlpI GC'TnA_GC
Cuts at: 2169 2169
Size: 5057
Bme1580I G_kGCm'C
Cuts at: 666 734 1978 2682 2775 3831 666
Size: 68 1244 704 93 1056 1892
Fragments arranged by size:
1892 1244 1056 704 93 68
BmrI ACTGGGnnnn_n'
Cuts at: 721 1964 2269 2350 2685 4829 721
Size: 1243 305 81 335 2144 949
Fragments arranged by size:
2144 1243 949 335 305 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 313 780 3424 313
Size: 467 2644 1946
Fragments arranged by size:
2644 1946 467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 685 788 1111 1145 1640 425
Size: 260 103 323 34 495 3842
Fragments arranged by size:
3842 495 323 260 103 34
Bpu10I CC'TnA_GC
Cuts at: 1790 1790
Size: 5057
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1010 1217 1878 2233 3112 3604 3902 1010
Size: 207 661 355 879 492 298 2165
Fragments arranged by size:
2165 879 661 492 355 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 327 489 1492 4761 327
Size: 162 1003 3269 623
Fragments arranged by size:
3269 1003 623 162
BsaAI yAC'GTr
Cuts at: 1973 2410 1973
Size: 437 4620
BsaBI GATnn'nnATC
Cuts at: 6 2571 4608 6
Size: 2565 2037 455
Fragments arranged by size:
2565 2037 455
BsaHI Gr'CG_yC
Cuts at: 2488 2736 3438 4934 2488
Size: 248 702 1496 2611
Fragments arranged by size:
2611 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1481 1772 2246 2767 3445 3719 3866 4277 1481
Size: 291 474 521 678 274 147 411 2261
Fragments arranged by size:
2261 678 521 474 411 291 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 562 592 887 917 562
Size: 30 295 30 4702
Fragments arranged by size:
4702 295 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 562 709 774 466
Size: 96 147 65 4749
Fragments arranged by size:
4749 147 96 65
BseYI C'CCAG_C
Cuts at: 419 2482 3240 3817 4313 419
Size: 2063 758 577 496 1163
Fragments arranged by size:
2063 1163 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2599 2645 3853 104
Size: 2495 46 1208 1308
Fragments arranged by size:
2495 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 701 766 2071 2849 3039 3831 701
Size: 65 1305 778 190 792 1927
Fragments arranged by size:
1927 1305 792 778 190 65
BsmBI CGTCTCn'nnnn_
Cuts at: 544 544
Size: 5057
Bsp1286I G_dGCh'C
Cuts at: 652 666 701 734 766 1978 2071 2682 2775
Size: 14 35 33 32 1212 93 611 93
Cuts at: 2775 2849 3039 3101 3831 652
Size: 74 190 62 730 1878
Fragments arranged by size:
1878 1212 730 611 190 93 93 74
62 35 33 32 14
BspEI T'CCGG_A
Cuts at: 4277 4277
Size: 5057
BspHI T'CATG_A
Cuts at: 1296 4233 1296
Size: 2937 2120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2107 2321 2623 3004 2107
Size: 214 302 381 4160
Fragments arranged by size:
4160 381 302 214
BsrBI CCG'CTC
Cuts at: 50 568 3349 3403 4887 5052 50
Size: 518 2781 54 1484 165 55
Fragments arranged by size:
2781 1484 518 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2969 2969
Size: 5057
BsrFI r'CCGG_y
Cuts at: 666 3055 3236 3445 666
Size: 2389 181 209 2278
Fragments arranged by size:
2389 2278 209 181
BssSI C'ACGA_G
Cuts at: 3328 3686 3328
Size: 358 4699
BstAPI GCAn_nnn'nTGC
Cuts at: 1814 2434 1814
Size: 620 4437
BstBI TT'CG_AA
Cuts at: 3418 3418
Size: 5057
BstXI CCAn_nnnn'nTGG
Cuts at: 506 506
Size: 5057
BstYI r'GATC_y
Cuts at: 1 138 359 597 654 965 1101 2082 2549
Size: 137 221 238 57 311 136 981 467
Cuts at: 2549 2572 2907 3153 4165 4251 4263 4609 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 981 467 449 346 335 311 246
238 221 137 136 86 57 23 12
Bsu36I CC'TnA_GG
Cuts at: 632 1016 1354 632
Size: 384 338 4335
Fragments arranged by size:
4335 384 338
BtgI C'CryG_G
Cuts at: 107 119 107
Size: 12 5045
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3007 3188 3351 3007
Size: 181 163 4713
Fragments arranged by size:
4713 181 163
BtsI GCAGTG_nn'
Cuts at: 464 2300 464
Size: 1836 3221
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1881 1916 1881
Size: 35 5022
DraI TTT'AAA
Cuts at: 2056 2056
Size: 5057
DrdI GACnn_nn'nnGTC
Cuts at: 1930 2763 3621 1930
Size: 833 858 3366
Fragments arranged by size:
3366 858 833
EaeI y'GGCC_r
Cuts at: 196 436 528 833 2642 2816 3207 3234 4579
Size: 240 92 305 1809 174 391 27 1345
Cuts at: 4579 196
Size: 674
Fragments arranged by size:
1809 1345 674 391 305 240 174 92
27
EagI C'GGCC_G
Cuts at: 2642 2642
Size: 5057
EarI CTCTTCn'nnn_
Cuts at: 146 686 794 980 1076 1490 1900 3080 3290
Size: 540 108 186 96 414 410 1180 210
Cuts at: 3290 146
Size: 1913
Fragments arranged by size:
1913 1180 540 414 410 210 186 108
96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 360 3265 3575 3721 4867 360
Size: 2905 310 146 1146 550
Fragments arranged by size:
2905 1146 550 310 146
EcoICRI GAG'CTC
Cuts at: 699 764 2069 699
Size: 65 1305 3687
Fragments arranged by size:
3687 1305 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 685 788 1111 1145 1571 1640 1644 1728
Size: 260 103 323 34 426 69 4 84
Cuts at: 1728 1896 1980 2882 3314 4061 4329 425
Size: 168 84 902 432 747 268 1153
Fragments arranged by size:
1153 902 747 432 426 323 268 260
168 103 84 84 69 34 4
EcoNI CCTnn'n_nnAGG
Cuts at: 544 1875 544
Size: 1331 3726
EcoO109I rG'GnC_Cy
Cuts at: 723 738 996 1880 2030 2196 723
Size: 15 258 884 150 166 3584
Fragments arranged by size:
3584 884 258 166 150 15
EcoRI G'AATT_C
Cuts at: 158 2061 158
Size: 1903 3154
EcoRV GAT'ATC
Cuts at: 1005 2008 1005
Size: 1003 4054
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 5057
FspI TGC'GCA
Cuts at: 2838 2838
Size: 5057
HaeII r_GCGC'y
Cuts at: 1824 2491 2739 3761 4740 1824
Size: 667 248 1022 979 2141
Fragments arranged by size:
2141 1022 979 667 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 639 671 771 803 887 919 952
Size: 32 45 32 100 32 84 32 33
Cuts at: 952 984 1899 1931 2899 2929 2931 2961 562
Size: 32 915 32 968 30 2 30 2658
Fragments arranged by size:
2658 968 915 100 84 45 33 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1679 2096 1679
Size: 417 4640
HindIII A'AGCT_T
Cuts at: 1656 1992 1656
Size: 336 4721
HpaI GTT'AAC
Cuts at: 1679 1679
Size: 5057
KasI G'GCGC_C
Cuts at: 2487 2735 2487
Size: 248 4809
KpnI G_GTAC'C
Cuts at: 1764 2077 1764
Size: 313 4744
MluI A'CGCG_T
Cuts at: 4490 4490
Size: 5057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 279 3728 3912 279
Size: 3449 184 1424
Fragments arranged by size:
3449 1424 184
MscI TGG'CCA
Cuts at: 2818 2818
Size: 5057
MslI CAynn'nnrTG
Cuts at: 1971 3046 3173 1971
Size: 1075 127 3855
Fragments arranged by size:
3855 1075 127
MspA1I CmG'CkG
Cuts at: 1580 2168 2482 2842 3855 4100 1580
Size: 588 314 360 1013 245 2537
Fragments arranged by size:
2537 1013 588 360 314 245
NaeI GCC'GGC
Cuts at: 3238 3238
Size: 5057
NarI GG'CG_CC
Cuts at: 2488 2736 2488
Size: 248 4809
NcoI C'CATG_G
Cuts at: 107 107
Size: 5057
NgoMIV G'CCGG_C
Cuts at: 3236 3236
Size: 5057
NspI r_CATG'y
Cuts at: 2110 2610 3517 2110
Size: 500 907 3650
Fragments arranged by size:
3650 907 500
PciI A'CATG_T
Cuts at: 3513 3513
Size: 5057
PflMI CCAn_nnn'nTGG
Cuts at: 445 445
Size: 5057
PmeI GTTT'AAAC
Cuts at: 2056 2056
Size: 5057
PmlI CAC'GTG
Cuts at: 1973 1973
Size: 5057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1787 1819 4196 4228 1787
Size: 32 2377 32 2616
Fragments arranged by size:
2616 2377 32 32
PpuMI rG'GwC_Cy
Cuts at: 723 738 996 2030 723
Size: 15 258 1034 3750
Fragments arranged by size:
3750 1034 258 15
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1447 1479 1447
Size: 32 5025
PstI C_TGCA'G
Cuts at: 596 713 756 1810 2104 2789 596
Size: 117 43 1054 294 685 2864
Fragments arranged by size:
2864 1054 685 294 117 43
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5057
PvuII CAG'CTG
Cuts at: 2482 2842 2482
Size: 360 4697
RsrII CG'GwC_CG
Cuts at: 3252 3252
Size: 5057
SacI G_AGCT'C
Cuts at: 701 766 2071 701
Size: 65 1305 3687
Fragments arranged by size:
3687 1305 65
SalI G'TCGA_C
Cuts at: 2094 2094
Size: 5057
SanDI GG'GwC_CC
Cuts at: 996 996
Size: 5057
SapI GCTCTTCn'nnn_
Cuts at: 794 980 1900 3080 3290 794
Size: 186 920 1180 210 2561
Fragments arranged by size:
2561 1180 920 210 186
SbfI CC_TGCA'GG
Cuts at: 713 2104 713
Size: 1391 3666
ScaI AGT'ACT
Cuts at: 4574 4574
Size: 5057
SfcI C'TryA_G
Cuts at: 33 113 412 592 709 752 1548 1806 2100
Size: 80 299 180 117 43 796 258 294
Cuts at: 2100 2785 3778 3969 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 796 685 299 294 258 191
180 117 80 43
SfoI GGC'GCC
Cuts at: 2489 2737 2489
Size: 248 4809
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5057
SmaI CCC'GGG
Cuts at: 2079 2079
Size: 5057
SmlI C'TyrA_G
Cuts at: 1025 1232 1857 2212 3127 3619 3881 1025
Size: 207 625 355 915 492 262 2201
Fragments arranged by size:
2201 915 625 492 355 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5057
SphI G_CATG'C
Cuts at: 2110 2610 2110
Size: 500 4557
SspI AAT'ATT
Cuts at: 1227 1227
Size: 5057
StuI AGG'CCT
Cuts at: 1128 1128
Size: 5057
StyI C'CwwG_G
Cuts at: 107 499 991 2191 2545 107
Size: 392 492 1200 354 2619
Fragments arranged by size:
2619 1200 492 392 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 411 498 1846 2640 2773 3232 3441 411
Size: 87 1348 794 133 459 209 2027
Fragments arranged by size:
2027 1348 794 459 209 133 87
TatI w'GTAC_w
Cuts at: 1639 4572 1639
Size: 2933 2124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4574 4606 4574
Size: 32 5025
Tth111I GACn'n_nGTC
Cuts at: 1594 2854 1594
Size: 1260 3797
XbaI T'CTAG_A
Cuts at: 2088 2088
Size: 5057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2552 2552
Size: 5057
XmaI C'CCGG_G
Cuts at: 2077 2077
Size: 5057
XmnI GAAnn'nnTTC
Cuts at: 386 886 1909 1966 386
Size: 500 1023 57 3477
Fragments arranged by size:
3477 1023 500 57
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI DraIII FseI FspAI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspOMI
PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI