Restriction map for
pF1KB3008
(Circular) (Six-base) MAPSORT of: pF1KB3008.seq Check: 2634 from: 1 to: 5579
pF1KB3008 5579 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 732 2617 732
Size: 1885 3694
Acc65I G'GTAC_C
Cuts at: 2595 2595
Size: 5579
AclI AA'CG_TT
Cuts at: 5419 5419
Size: 5579
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1162 2432 3404 3836 4583 4851 1162
Size: 1270 972 432 747 268 1890
Fragments arranged by size:
1890 1270 972 747 432 268
AflIII A'CryG_T
Cuts at: 2071 2931 4035 5012 2071
Size: 860 1104 977 2638
Fragments arranged by size:
2638 1104 977 860
AgeI A'CCGG_T
Cuts at: 3967 3967
Size: 5579
AhdI GACnn_n'nnGTC
Cuts at: 2207 2207
Size: 5579
AleI CACnn'nnGTG
Cuts at: 561 784 561
Size: 223 5356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 579 2128 579
Size: 1549 4030
AlwNI CAG_nnn'CTG
Cuts at: 1483 1885 2002 2038 2884 4451 1483
Size: 402 117 36 846 1567 2611
Fragments arranged by size:
2611 1567 846 402 117 36
ApaLI G'TGCA_C
Cuts at: 2363 4349 2363
Size: 1986 3593
ApoI r'AATT_y
Cuts at: 486 949 1087 2230 2583 4932 5188 486
Size: 463 138 1143 353 2349 256 877
Fragments arranged by size:
2349 1143 877 463 353 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 5579
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5579
AvaI C'yCGr_G
Cuts at: 2599 2599
Size: 5579
BamHI G'GATC_C
Cuts at: 1609 2604 1609
Size: 995 4584
BanI G'GyrC_C
Cuts at: 2595 3009 3257 3292 2595
Size: 414 248 35 4882
Fragments arranged by size:
4882 414 248 35
BanII G_rGCy'C
Cuts at: 753 1424 1757 2593 3623 753
Size: 671 333 836 1030 2709
Fragments arranged by size:
2709 1030 836 671 333
BbeI G_GCGC'C
Cuts at: 3013 3261 3013
Size: 248 5331
BbsI GAAGACnn'nnnn_
Cuts at: 2305 2508 2305
Size: 203 5376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1561 1595 1561
Size: 34 5545
BciVI GTATCCnnnnn_n'
Cuts at: 3477 4238 5579 3477
Size: 761 1341 3477
Fragments arranged by size:
3477 1341 761
BclI T'GATC_A
Cuts at: 350 3099 350
Size: 2749 2830
BfrBI ATG'CAT
Cuts at: 528 528
Size: 5579
BglII A'GATC_T
Cuts at: 591 1461 3094 5131 591
Size: 870 1633 2037 1039
Fragments arranged by size:
2037 1633 1039 870
BlpI GC'TnA_GC
Cuts at: 2691 2691
Size: 5579
Bme1580I G_kGCm'C
Cuts at: 2367 3204 3297 4353 2367
Size: 837 93 1056 3593
Fragments arranged by size:
3593 1056 837 93
BmgBI CAC'GTC
Cuts at: 2074 2303 2074
Size: 229 5350
BmrI ACTGGGnnnn_n'
Cuts at: 128 2791 2872 3207 5351 128
Size: 2663 81 335 2144 356
Fragments arranged by size:
2663 2144 356 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2237 3946 2237
Size: 1709 3870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1862 2015 2073 502
Size: 1360 153 58 4008
Fragments arranged by size:
4008 1360 153 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 2755 3634 4126 4424 398
Size: 2357 879 492 298 1553
Fragments arranged by size:
2357 1553 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1866 5283 1866
Size: 3417 2162
BsaAI yAC'GTr
Cuts at: 2932 2932
Size: 5579
BsaBI GATnn'nnATC
Cuts at: 6 596 1466 3093 5130 6
Size: 590 870 1627 2037 455
Fragments arranged by size:
2037 1627 870 590 455
BsaHI Gr'CG_yC
Cuts at: 698 3010 3258 3960 5456 698
Size: 2312 248 702 1496 821
Fragments arranged by size:
2312 1496 821 702 248
BsaWI w'CCGG_w
Cuts at: 2142 2768 3289 3967 4241 4388 4799 2142
Size: 626 521 678 274 147 411 2922
Fragments arranged by size:
2922 678 626 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1034 1064 1983 2013 2071 2101 1034
Size: 30 919 30 58 30 4512
Fragments arranged by size:
4512 919 58 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1976 1976
Size: 5579
BseYI C'CCAG_C
Cuts at: 1415 2058 3004 3762 4339 4835 1415
Size: 643 946 758 577 496 2159
Fragments arranged by size:
2159 946 758 643 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 1234
Size: 5579
BsiEI CG_ry'CG
Cuts at: 104 3121 3167 4375 104
Size: 3017 46 1208 1308
Fragments arranged by size:
3017 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 753 1757 2367 2593 3371 3561 4353 753
Size: 1004 610 226 778 190 792 1979
Fragments arranged by size:
1979 1004 792 778 610 226 190
BsmI GAATG_Cn'
Cuts at: 528 1542 528
Size: 1014 4565
Bsp1286I G_dGCh'C
Cuts at: 753 1424 1757 2367 2593 3204 3297 3371 3561
Size: 671 333 610 226 611 93 74 190
Cuts at: 3561 3623 4353 753
Size: 62 730 1979
Fragments arranged by size:
1979 730 671 611 610 333 226 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4799 4799
Size: 5579
BspHI T'CATG_A
Cuts at: 864 4755 864
Size: 3891 1688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2629 2843 3145 3526 2629
Size: 214 302 381 4682
Fragments arranged by size:
4682 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2221 3871 3925 5409 5574 50
Size: 2171 1650 54 1484 165 55
Fragments arranged by size:
2171 1650 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1416 1810 3491 1416
Size: 394 1681 3504
Fragments arranged by size:
3504 1681 394
BsrFI r'CCGG_y
Cuts at: 3577 3758 3967 3577
Size: 181 209 5189
Fragments arranged by size:
5189 209 181
BsrGI T'GTAC_A
Cuts at: 2442 2442
Size: 5579
BssSI C'ACGA_G
Cuts at: 3850 4208 3850
Size: 358 5221
BstAPI GCAn_nnn'nTGC
Cuts at: 2253 2956 2253
Size: 703 4876
BstBI TT'CG_AA
Cuts at: 1818 3940 1818
Size: 2122 3457
BstYI r'GATC_y
Cuts at: 1 591 1461 1609 2604 3071 3094 3429 3675
Size: 590 870 148 995 467 23 335 246
Cuts at: 3675 4687 4773 4785 5131 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 995 870 590 467 449 346 335
246 148 86 23 12
BstZ17I GTA'TAC
Cuts at: 733 733
Size: 5579
Bsu36I CC'TnA_GG
Cuts at: 703 703
Size: 5579
BtgI C'CryG_G
Cuts at: 2242 2242
Size: 5579
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3529 3710 3873 3529
Size: 181 163 5235
Fragments arranged by size:
5235 181 163
BtsI GCAGTG_nn'
Cuts at: 285 510 2475 2822 285
Size: 225 1965 347 3042
Fragments arranged by size:
3042 1965 347 225
DraI TTT'AAA
Cuts at: 497 2578 497
Size: 2081 3498
DrdI GACnn_nn'nnGTC
Cuts at: 3285 4143 3285
Size: 858 4721
EaeI y'GGCC_r
Cuts at: 1133 1366 3164 3338 3729 3756 5101 1133
Size: 233 1798 174 391 27 1345 1611
Fragments arranged by size:
1798 1611 1345 391 233 174 27
EagI C'GGCC_G
Cuts at: 3164 3164
Size: 5579
EarI CTCTTCn'nnn_
Cuts at: 607 679 3602 3812 607
Size: 72 2923 210 2374
Fragments arranged by size:
2923 2374 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3787 4097 4243 5389 3787
Size: 310 146 1146 3977
Fragments arranged by size:
3977 1146 310 146
EcoICRI GAG'CTC
Cuts at: 751 1755 2591 751
Size: 1004 836 3739
Fragments arranged by size:
3739 1004 836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1162 1862 2015 2073 2432 3404 3836 4583
Size: 660 700 153 58 359 972 432 747
Cuts at: 4583 4851 502
Size: 268 1230
Fragments arranged by size:
1230 972 747 700 660 432 359 268
153 58
EcoO109I rG'GnC_Cy
Cuts at: 234 1228 2055 2718 234
Size: 994 827 663 3095
Fragments arranged by size:
3095 994 827 663
EcoRI G'AATT_C
Cuts at: 2583 2583
Size: 5579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1746 3598 1746
Size: 1852 3727
FspI TGC'GCA
Cuts at: 3360 3360
Size: 5579
HaeII r_GCGC'y
Cuts at: 3013 3261 4283 5262 3013
Size: 248 1022 979 3330
Fragments arranged by size:
3330 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 2193 2225 3421 3451 3453 3483 451
Size: 32 1710 32 1196 30 2 30 2547
Fragments arranged by size:
2547 1710 1196 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2618 2618
Size: 5579
KasI G'GCGC_C
Cuts at: 3009 3257 3009
Size: 248 5331
KpnI G_GTAC'C
Cuts at: 2599 2599
Size: 5579
MfeI C'AATT_G
Cuts at: 1277 1277
Size: 5579
MluI A'CGCG_T
Cuts at: 5012 5012
Size: 5579
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2014 2191 4250 4434 2014
Size: 177 2059 184 3159
Fragments arranged by size:
3159 2059 184 177
MscI TGG'CCA
Cuts at: 1135 1368 3340 1135
Size: 233 1972 3374
Fragments arranged by size:
3374 1972 233
MslI CAynn'nnrTG
Cuts at: 561 784 1547 3568 3695 561
Size: 223 763 2021 127 2445
Fragments arranged by size:
2445 2021 763 223 127
MspA1I CmG'CkG
Cuts at: 1320 1960 2690 3004 3364 4377 4622 1320
Size: 640 730 314 360 1013 245 2277
Fragments arranged by size:
2277 1013 730 640 360 314 245
NaeI GCC'GGC
Cuts at: 3760 3760
Size: 5579
NarI GG'CG_CC
Cuts at: 3010 3258 3010
Size: 248 5331
NdeI CA'TA_TG
Cuts at: 1392 1392
Size: 5579
NgoMIV G'CCGG_C
Cuts at: 3758 3758
Size: 5579
NsiI A_TGCA'T
Cuts at: 530 530
Size: 5579
NspI r_CATG'y
Cuts at: 2632 3132 4039 2632
Size: 500 907 4172
Fragments arranged by size:
4172 907 500
PasI CC'CwG_GG
Cuts at: 769 769
Size: 5579
PciI A'CATG_T
Cuts at: 4035 4035
Size: 5579
PflMI CCAn_nnn'nTGG
Cuts at: 936 1573 1885 936
Size: 637 312 4630
Fragments arranged by size:
4630 637 312
PfoI T'CCnGG_A
Cuts at: 329 329
Size: 5579
PmeI GTTT'AAAC
Cuts at: 2578 2578
Size: 5579
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 4718
Size: 32 5547
PpuMI rG'GwC_Cy
Cuts at: 1228 2055 1228
Size: 827 4752
PstI C_TGCA'G
Cuts at: 1173 2626 3311 1173
Size: 1453 685 3441
Fragments arranged by size:
3441 1453 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5579
PvuII CAG'CTG
Cuts at: 1320 1960 3004 3364 1320
Size: 640 1044 360 3535
Fragments arranged by size:
3535 1044 640 360
RsrII CG'GwC_CG
Cuts at: 3774 3774
Size: 5579
SacI G_AGCT'C
Cuts at: 753 1757 2593 753
Size: 1004 836 3739
Fragments arranged by size:
3739 1004 836
SalI G'TCGA_C
Cuts at: 2616 2616
Size: 5579
SapI GCTCTTCn'nnn_
Cuts at: 3602 3812 3602
Size: 210 5369
SbfI CC_TGCA'GG
Cuts at: 2626 2626
Size: 5579
ScaI AGT'ACT
Cuts at: 1250 5096 1250
Size: 3846 1733
SfcI C'TryA_G
Cuts at: 33 1169 2427 2622 3307 4300 4491 33
Size: 1136 1258 195 685 993 191 1121
Fragments arranged by size:
1258 1136 1121 993 685 195 191
SfoI GGC'GCC
Cuts at: 3011 3259 3011
Size: 248 5331
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5579
SmaI CCC'GGG
Cuts at: 2601 2601
Size: 5579
SmlI C'TyrA_G
Cuts at: 413 2734 3649 4141 4403 413
Size: 2321 915 492 262 1589
Fragments arranged by size:
2321 1589 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5579
SphI G_CATG'C
Cuts at: 2632 3132 2632
Size: 500 5079
StyI C'CwwG_G
Cuts at: 395 834 853 1623 2713 3067 395
Size: 439 19 770 1090 354 2907
Fragments arranged by size:
2907 1090 770 439 354 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 759 796 2165 3162 3295 3754 3963 759
Size: 37 1369 997 133 459 209 2375
Fragments arranged by size:
2375 1369 997 459 209 133 37
TatI w'GTAC_w
Cuts at: 1248 2442 5094 1248
Size: 1194 2652 1733
Fragments arranged by size:
2652 1733 1194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1734 1766 5096 5128 1734
Size: 32 3330 32 2185
Fragments arranged by size:
3330 2185 32 32
Tth111I GACn'n_nGTC
Cuts at: 271 3376 271
Size: 3105 2474
XbaI T'CTAG_A
Cuts at: 2610 2610
Size: 5579
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3074 3074
Size: 5579
XmaI C'CCGG_G
Cuts at: 2599 2599
Size: 5579
XmnI GAAnn'nnTTC
Cuts at: 2230 2230
Size: 5579
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BbvCI BglI BmtI Bpu10I BsiWI BsmBI BssHII
BstEII BstXI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NcoI NheI NotI NruI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI