(Circular) (Six-base) MAPSORT of: pF1KB2501.seq Check: 3229 from: 1 to: 5027 pF1KB2501 5027 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AccI GT'mk_AC Cuts at: 2065 2065 Size: 5027 Acc65I G'GTAC_C Cuts at: 2043 2043 Size: 5027 AclI AA'CG_TT Cuts at: 4867 4867 Size: 5027 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1331 2852 3284 3637 4299 1331 Size: 1521 432 353 662 2059 Fragments arranged by size: 2059 1521 662 432 353 AflIII A'CryG_T Cuts at: 491 1678 1828 1856 1858 2379 4179 4460 491 Size: 1187 150 28 2 521 1800 281 1058 Fragments arranged by size: 1800 1187 1058 521 281 150 28 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 510 510 Size: 5027 AlwNI CAG_nnn'CTG Cuts at: 1516 2332 3770 1516 Size: 816 1438 2773 Fragments arranged by size: 2773 1438 816 ApaI G_GGCC'C Cuts at: 709 759 709 Size: 50 4977 ApaLI G'TGCA_C Cuts at: 3865 3865 Size: 5027 ApoI r'AATT_y Cuts at: 994 1033 1178 1249 2031 4380 4636 994 Size: 39 145 71 782 2349 256 1385 Fragments arranged by size: 2349 1385 782 256 145 71 39 AseI AT'TA_AT Cuts at: 20 20 Size: 5027 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5027 AvaI C'yCGr_G Cuts at: 322 429 700 2047 322 Size: 107 271 1347 3302 Fragments arranged by size: 3302 1347 271 107 BamHI G'GATC_C Cuts at: 942 1169 2052 942 Size: 227 883 3917 Fragments arranged by size: 3917 883 227 BanI G'GyrC_C Cuts at: 154 425 2043 2457 2705 2740 154 Size: 271 1618 414 248 35 2441 Fragments arranged by size: 2441 1618 414 271 248 35 BanII G_rGCy'C Cuts at: 233 557 701 709 759 1332 1473 2041 3071 Size: 324 144 8 50 573 141 568 1030 Cuts at: 3071 233 Size: 2189 Fragments arranged by size: 2189 1030 573 568 324 144 141 50 8 BbeI G_GCGC'C Cuts at: 2461 2709 2461 Size: 248 4779 BbsI GAAGACnn'nnnn_ Cuts at: 429 773 429 Size: 344 4683 BciVI GTATCCnnnnn_n' Cuts at: 2925 3981 5027 2925 Size: 1056 1046 2925 Fragments arranged by size: 2925 1056 1046 BclI T'GATC_A Cuts at: 2547 2547 Size: 5027 BglI GCCn_nnn'nGGC Cuts at: 598 713 598 Size: 115 4912 BglII A'GATC_T Cuts at: 2542 4579 2542 Size: 2037 2990 BlpI GC'TnA_GC Cuts at: 2139 2139 Size: 5027 Bme1580I G_kGCm'C Cuts at: 157 709 759 2652 2745 3869 157 Size: 552 50 1893 93 1124 1315 Fragments arranged by size: 1893 1315 1124 552 93 50 BmgBI CAC'GTC Cuts at: 492 549 492 Size: 57 4970 BmrI ACTGGGnnnn_n' Cuts at: 2239 2320 2655 4799 2239 Size: 81 335 2144 2467 Fragments arranged by size: 2467 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1967 2012 3394 1967 Size: 45 1382 3600 Fragments arranged by size: 3600 1382 45 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 750 1508 1937 750 Size: 758 429 3840 Fragments arranged by size: 3840 758 429 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 169 2203 3082 3796 4094 169 Size: 2034 879 714 298 1102 Fragments arranged by size: 2034 1102 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 535 610 4731 535 Size: 75 4121 831 Fragments arranged by size: 4121 831 75 BsaAI yAC'GTr Cuts at: 1859 2380 1859 Size: 521 4506 BsaBI GATnn'nnATC Cuts at: 6 2541 4578 6 Size: 2535 2037 455 Fragments arranged by size: 2535 2037 455 BsaHI Gr'CG_yC Cuts at: 434 590 2458 2706 3408 4904 434 Size: 156 1868 248 702 1496 557 Fragments arranged by size: 1868 1496 702 557 248 156 BsaWI w'CCGG_w Cuts at: 2216 2737 3415 3826 3973 4247 2216 Size: 521 678 411 147 274 2996 Fragments arranged by size: 2996 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 959 989 959 Size: 30 4997 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 213 526 529 610 747 1598 213 Size: 313 3 81 137 851 3642 Fragments arranged by size: 3642 851 313 137 81 3 BseYI C'CCAG_C Cuts at: 675 2452 3210 3875 4283 675 Size: 1777 758 665 408 1419 Fragments arranged by size: 1777 1419 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 460 460 Size: 5027 BsiEI CG_ry'CG Cuts at: 104 405 423 2569 2615 3845 104 Size: 301 18 2146 46 1230 1286 Fragments arranged by size: 2146 1286 1230 301 46 18 BsiHKAI G_wGCw'C Cuts at: 452 1332 1361 1482 2041 2819 3009 3869 452 Size: 880 29 121 559 778 190 860 1610 Fragments arranged by size: 1610 880 860 778 559 190 121 29 Bsp1286I G_dGCh'C Cuts at: 157 233 452 557 701 709 759 1332 1361 Size: 76 219 105 144 8 50 573 29 Cuts at: 1361 1473 1482 2041 2652 2745 2819 3009 3071 Size: 112 9 559 611 93 74 190 62 Cuts at: 3071 3869 157 Size: 798 1315 Fragments arranged by size: 1315 798 611 573 559 219 190 144 112 105 93 76 74 62 50 29 9 8 BspHI T'CATG_A Cuts at: 3459 3459 Size: 5027 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2077 2291 2593 2974 2077 Size: 214 302 381 4130 Fragments arranged by size: 4130 381 302 214 BsrBI CCG'CTC Cuts at: 50 504 3319 3373 4857 5022 50 Size: 454 2815 54 1484 165 55 Fragments arranged by size: 2815 1484 454 165 55 54 BsrDI GCAATG_nn' Cuts at: 1562 1941 2939 1562 Size: 379 998 3650 Fragments arranged by size: 3650 998 379 BsrFI r'CCGG_y Cuts at: 422 466 575 3025 3206 422 Size: 44 109 2450 181 2243 Fragments arranged by size: 2450 2243 181 109 44 BssSI C'ACGA_G Cuts at: 3298 4006 3298 Size: 708 4319 BstAPI GCAn_nnn'nTGC Cuts at: 1936 2404 1936 Size: 468 4559 BstBI TT'CG_AA Cuts at: 764 3388 764 Size: 2624 2403 BstXI CCAn_nnnn'nTGG Cuts at: 397 675 397 Size: 278 4749 BstYI r'GATC_y Cuts at: 1 942 1169 2052 2519 2542 2877 3123 3429 Size: 941 227 883 467 23 335 246 306 Cuts at: 3429 3441 3527 4579 1 Size: 12 86 1052 449 Fragments arranged by size: 1052 941 883 467 449 335 306 246 227 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1147 1610 1147 Size: 463 4564 BtgI C'CryG_G Cuts at: 107 251 369 107 Size: 144 118 4765 Fragments arranged by size: 4765 144 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2977 3158 3321 2977 Size: 181 163 4683 Fragments arranged by size: 4683 181 163 BtsI GCAGTG_nn' Cuts at: 477 2270 477 Size: 1793 3234 DraI TTT'AAA Cuts at: 2026 2026 Size: 5027 DraIII CAC_nnn'GTG Cuts at: 1672 1672 Size: 5027 DrdI GACnn_nn'nnGTC Cuts at: 2733 4077 2733 Size: 1344 3683 EaeI y'GGCC_r Cuts at: 402 665 723 1070 2612 2786 3177 3204 4549 Size: 263 58 347 1542 174 391 27 1345 Cuts at: 4549 402 Size: 880 Fragments arranged by size: 1542 1345 880 391 347 263 174 58 27 EagI C'GGCC_G Cuts at: 402 2612 402 Size: 2210 2817 EarI CTCTTCn'nnn_ Cuts at: 511 766 1235 1812 3050 3260 511 Size: 255 469 577 1238 210 2278 Fragments arranged by size: 2278 1238 577 469 255 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3235 3977 4123 4837 3235 Size: 742 146 714 3425 Fragments arranged by size: 3425 742 714 146 EcoICRI GAG'CTC Cuts at: 1330 2039 1330 Size: 709 4318 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 750 1331 1508 1937 2852 3284 3637 4299 750 Size: 581 177 429 915 432 353 662 1478 Fragments arranged by size: 1478 915 662 581 432 429 353 177 EcoO109I rG'GnC_Cy Cuts at: 657 705 706 756 2166 657 Size: 48 1 50 1410 3518 Fragments arranged by size: 3518 1410 50 48 1 EcoRI G'AATT_C Cuts at: 1033 2031 1033 Size: 998 4029 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1153 3046 1153 Size: 1893 3134 FspI TGC'GCA Cuts at: 2808 2808 Size: 5027 HaeII r_GCGC'y Cuts at: 310 604 1995 2461 2709 3939 4710 310 Size: 294 1391 466 248 1230 771 627 Fragments arranged by size: 1391 1230 771 627 466 294 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 703 735 760 792 1991 2023 2869 2899 2901 Size: 32 25 32 1199 32 846 30 2 Cuts at: 2901 2931 703 Size: 30 2799 Fragments arranged by size: 2799 1199 846 32 32 32 30 30 25 2 HincII GTy'rAC Cuts at: 2066 2066 Size: 5027 HindIII A'AGCT_T Cuts at: 863 1905 863 Size: 1042 3985 KasI G'GCGC_C Cuts at: 2457 2705 2457 Size: 248 4779 KpnI G_GTAC'C Cuts at: 2047 2047 Size: 5027 MluI A'CGCG_T Cuts at: 4460 4460 Size: 5027 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3786 3970 3786 Size: 184 4843 MscI TGG'CCA Cuts at: 1072 2788 1072 Size: 1716 3311 MslI CAynn'nnrTG Cuts at: 395 1462 1510 3016 3143 395 Size: 1067 48 1506 127 2279 Fragments arranged by size: 2279 1506 1067 127 48 MspA1I CmG'CkG Cuts at: 315 371 728 2138 2452 2812 3596 3841 315 Size: 56 357 1410 314 360 784 245 1501 Fragments arranged by size: 1501 1410 784 360 357 314 245 56 NaeI GCC'GGC Cuts at: 3208 3208 Size: 5027 NarI GG'CG_CC Cuts at: 2458 2706 2458 Size: 248 4779 NcoI C'CATG_G Cuts at: 107 107 Size: 5027 NgoMIV G'CCGG_C Cuts at: 3206 3206 Size: 5027 NotI GC'GGCC_GC Cuts at: 402 402 Size: 5027 NspI r_CATG'y Cuts at: 753 1461 1682 1832 2080 2580 4183 753 Size: 708 221 150 248 500 1603 1597 Fragments arranged by size: 1603 1597 708 500 248 221 150 PasI CC'CwG_GG Cuts at: 1419 1419 Size: 5027 PciI A'CATG_T Cuts at: 1678 1828 4179 1678 Size: 150 2351 2526 Fragments arranged by size: 2526 2351 150 PmeI GTTT'AAAC Cuts at: 2026 2026 Size: 5027 PmlI CAC'GTG Cuts at: 1859 1859 Size: 5027 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3473 3505 3473 Size: 32 4995 PpuMI rG'GwC_Cy Cuts at: 657 657 Size: 5027 PshAI GACnn'nnGTC Cuts at: 1679 1679 Size: 5027 PspOMI G'GGCC_C Cuts at: 705 755 705 Size: 50 4977 PstI C_TGCA'G Cuts at: 272 364 743 2074 2759 272 Size: 92 379 1331 685 2540 Fragments arranged by size: 2540 1331 685 379 92 PvuI CG_AT'CG Cuts at: 104 104 Size: 5027 PvuII CAG'CTG Cuts at: 2452 2812 2452 Size: 360 4667 RsrII CG'GwC_CG Cuts at: 3222 3222 Size: 5027 SacI G_AGCT'C Cuts at: 1332 2041 1332 Size: 709 4318 SacII CC_GC'GG Cuts at: 372 372 Size: 5027 SalI G'TCGA_C Cuts at: 2064 2064 Size: 5027 SapI GCTCTTCn'nnn_ Cuts at: 511 3050 3260 511 Size: 2539 210 2278 Fragments arranged by size: 2539 2278 210 SbfI CC_TGCA'GG Cuts at: 2074 2074 Size: 5027 ScaI AGT'ACT Cuts at: 1242 4544 1242 Size: 3302 1725 SfcI C'TryA_G Cuts at: 33 268 360 739 922 1400 2070 2755 3723 Size: 235 92 379 183 478 670 685 968 Cuts at: 3723 3914 33 Size: 191 1146 Fragments arranged by size: 1146 968 685 670 478 379 235 191 183 92 SfoI GGC'GCC Cuts at: 2459 2707 2459 Size: 248 4779 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5027 SmaI CCC'GGG Cuts at: 324 431 2049 324 Size: 107 1618 3302 Fragments arranged by size: 3302 1618 107 SmlI C'TyrA_G Cuts at: 184 2182 3097 3811 4073 184 Size: 1998 915 714 262 1138 Fragments arranged by size: 1998 1138 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5027 SphI G_CATG'C Cuts at: 1461 2080 2580 1461 Size: 619 500 3908 Fragments arranged by size: 3908 619 500 SspI AAT'ATT Cuts at: 873 873 Size: 5027 StyI C'CwwG_G Cuts at: 107 1550 1576 2161 2515 107 Size: 1443 26 585 354 2619 Fragments arranged by size: 2619 1443 585 354 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 559 2610 2743 3202 3411 559 Size: 2051 133 459 209 2175 Fragments arranged by size: 2175 2051 459 209 133 TatI w'GTAC_w Cuts at: 1240 1541 1663 1741 4542 1240 Size: 301 122 78 2801 1725 Fragments arranged by size: 2801 1725 301 122 78 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 959 991 4544 4576 959 Size: 32 3553 32 1410 Fragments arranged by size: 3553 1410 32 32 Tth111I GACn'n_nGTC Cuts at: 2824 2824 Size: 5027 XbaI T'CTAG_A Cuts at: 2058 2058 Size: 5027 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1416 2522 1416 Size: 1106 3921 XmaI C'CCGG_G Cuts at: 322 429 2047 322 Size: 107 1618 3302 Fragments arranged by size: 3302 1618 107 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AleI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NruI NsiI PacI PflMI PfoI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI