Restriction map for
pF1KB2389
(Circular) (Six-base) MAPSORT of: pF1KB2389.seq Check: 9166 from: 1 to: 6287
pF1KB2389 6287 bp
With 182 enzymes: SgfI *
August 12, 2008 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1082 1218 1530 3145 1082
Size: 136 312 1615 4224
Fragments arranged by size:
4224 1615 312 136
AatII G_ACGT'C
Cuts at: 596 1499 596
Size: 903 5384
AccI GT'mk_AC
Cuts at: 2108 3325 2108
Size: 1217 5070
Acc65I G'GTAC_C
Cuts at: 3303 3303
Size: 6287
AclI AA'CG_TT
Cuts at: 6127 6127
Size: 6287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1155 1309 2083 2187 2214 2449 2833 3260 4112
Size: 154 774 104 27 235 384 427 852
Cuts at: 4112 4544 5291 5559 1155
Size: 432 747 268 1883
Fragments arranged by size:
1883 852 774 747 432 427 384 268
235 154 104 27
AfeI AGC'GCT
Cuts at: 535 3205 535
Size: 2670 3617
AflII C'TTAA_G
Cuts at: 255 255
Size: 6287
AflIII A'CryG_T
Cuts at: 228 546 731 2150 3639 4743 5720 228
Size: 318 185 1419 1489 1104 977 795
Fragments arranged by size:
1489 1419 1104 977 795 318 185
AgeI A'CCGG_T
Cuts at: 4675 4675
Size: 6287
AleI CACnn'nnGTG
Cuts at: 3049 3049
Size: 6287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2169 2169
Size: 6287
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 379 411 379
Size: 32 6255
AlwNI CAG_nnn'CTG
Cuts at: 492 972 2035 3018 3592 5159 492
Size: 480 1063 983 574 1567 1620
Fragments arranged by size:
1620 1567 1063 983 574 480
ApaI G_GGCC'C
Cuts at: 805 1446 805
Size: 641 5646
ApaLI G'TGCA_C
Cuts at: 1771 5057 1771
Size: 3286 3001
ApoI r'AATT_y
Cuts at: 2068 3291 5640 5896 2068
Size: 1223 2349 256 2459
Fragments arranged by size:
2459 2349 1223 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
AvaI C'yCGr_G
Cuts at: 804 3307 804
Size: 2503 3784
AvrII C'CTAG_G
Cuts at: 114 114
Size: 6287
BamHI G'GATC_C
Cuts at: 794 3312 794
Size: 2518 3769
BanI G'GyrC_C
Cuts at: 1825 2264 3248 3303 3717 3965 4000 1825
Size: 439 984 55 414 248 35 4112
Fragments arranged by size:
4112 984 439 414 248 55 35
BanII G_rGCy'C
Cuts at: 805 1446 1988 2312 2552 3301 4331 805
Size: 641 542 324 240 749 1030 2761
Fragments arranged by size:
2761 1030 749 641 542 324 240
BbeI G_GCGC'C
Cuts at: 3252 3721 3969 3252
Size: 469 248 5570
Fragments arranged by size:
5570 469 248
BbsI GAAGACnn'nnnn_
Cuts at: 395 578 1685 2507 395
Size: 183 1107 822 4175
Fragments arranged by size:
4175 1107 822 183
BbvCI CC'TCA_GC
Cuts at: 3224 3224
Size: 6287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1266 1300 1266
Size: 34 6253
BciVI GTATCCnnnnn_n'
Cuts at: 1621 4185 4946 6287 1621
Size: 2564 761 1341 1621
Fragments arranged by size:
2564 1621 1341 761
BclI T'GATC_A
Cuts at: 317 3807 317
Size: 3490 2797
BglI GCCn_nnn'nGGC
Cuts at: 835 1753 835
Size: 918 5369
BglII A'GATC_T
Cuts at: 263 2483 3802 5839 263
Size: 2220 1319 2037 711
Fragments arranged by size:
2220 2037 1319 711
BlpI GC'TnA_GC
Cuts at: 3399 3399
Size: 6287
Bme1580I G_kGCm'C
Cuts at: 739 805 1446 1775 2450 3195 3912 4005 5061
Size: 66 641 329 675 745 717 93 1056
Cuts at: 5061 739
Size: 1965
Fragments arranged by size:
1965 1056 745 717 675 641 329 93
66
BmgBI CAC'GTC
Cuts at: 576 2625 576
Size: 2049 4238
BmrI ACTGGGnnnn_n'
Cuts at: 231 642 1028 3499 3580 3915 6059 231
Size: 411 386 2471 81 335 2144 459
Fragments arranged by size:
2471 2144 459 411 386 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 285 2364 2529 4654 285
Size: 2079 165 2125 1918
Fragments arranged by size:
2125 2079 1918 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2158 2326 2565 2767 3169 2158
Size: 168 239 202 402 5276
Fragments arranged by size:
5276 402 239 202 168
Bpu10I CC'TnA_GC
Cuts at: 715 3224 715
Size: 2509 3778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2065 2106 3463 4342 4834 5132 2065
Size: 41 1357 879 492 298 3220
Fragments arranged by size:
3220 1357 879 492 298 41
BsaI GGTCTCn'nnnn_
Cuts at: 1033 1239 2335 5991 1033
Size: 206 1096 3656 1329
Fragments arranged by size:
3656 1329 1096 206
BsaAI yAC'GTr
Cuts at: 2922 3640 2922
Size: 718 5569
BsaBI GATnn'nnATC
Cuts at: 6 322 2128 2299 3801 5838 6
Size: 316 1806 171 1502 2037 455
Fragments arranged by size:
2037 1806 1502 455 316 171
BsaHI Gr'CG_yC
Cuts at: 593 1496 3249 3718 3966 4668 6164 593
Size: 903 1753 469 248 702 1496 716
Fragments arranged by size:
1753 1496 903 716 702 469 248
BsaWI w'CCGG_w
Cuts at: 3476 3997 4675 4949 5096 5507 3476
Size: 521 678 274 147 411 4256
Fragments arranged by size:
4256 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2248 2278 2299 2329 2248
Size: 30 21 30 6206
Fragments arranged by size:
6206 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 258 1108 2188 2653 2749 2923 258
Size: 850 1080 465 96 174 3622
Fragments arranged by size:
3622 1080 850 465 174 96
BseYI C'CCAG_C
Cuts at: 287 3712 4470 5047 5543 287
Size: 3425 758 577 496 1031
Fragments arranged by size:
3425 1031 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1821 1906 2718 3052 1051
Size: 770 85 812 334 4286
Fragments arranged by size:
4286 812 770 334 85
BsiEI CG_ry'CG
Cuts at: 104 3829 3875 5083 104
Size: 3725 46 1208 1308
Fragments arranged by size:
3725 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1112 1282 1775 1988 2312 3301 4079 4269 5061
Size: 170 493 213 324 989 778 190 792
Cuts at: 5061 1112
Size: 2338
Fragments arranged by size:
2338 989 792 778 493 324 213 190
170
BsmBI CGTCTCn'nnnn_
Cuts at: 2583 2583
Size: 6287
Bsp1286I G_dGCh'C
Cuts at: 739 805 1112 1282 1446 1775 1988 2312 2450
Size: 66 307 170 164 329 213 324 138
Cuts at: 2450 2552 3195 3301 3912 4005 4079 4269 4331
Size: 102 643 106 611 93 74 190 62
Cuts at: 4331 5061 739
Size: 730 1965
Fragments arranged by size:
1965 730 643 611 329 324 307 213
190 170 164 138 106 102 93 74
66 62
BspEI T'CCGG_A
Cuts at: 5507 5507
Size: 6287
BspHI T'CATG_A
Cuts at: 5463 5463
Size: 6287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 683 1082 1218 1530 1597 2188 3145 3337 3551
Size: 399 136 312 67 591 957 192 214
Cuts at: 3551 3853 4234 683
Size: 302 381 2736
Fragments arranged by size:
2736 957 591 399 381 312 302 214
192 136 67
BsrBI CCG'CTC
Cuts at: 50 4579 4633 6117 6282 50
Size: 4529 54 1484 165 55
Fragments arranged by size:
4529 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 665 1322 3218 4199 665
Size: 657 1896 981 2753
Fragments arranged by size:
2753 1896 981 657
BsrFI r'CCGG_y
Cuts at: 1145 4285 4466 4675 1145
Size: 3140 181 209 2757
Fragments arranged by size:
3140 2757 209 181
BssSI C'ACGA_G
Cuts at: 3196 4558 4916 3196
Size: 1362 358 4567
Fragments arranged by size:
4567 1362 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1074 1107 2688 3664 1074
Size: 33 1581 976 3697
Fragments arranged by size:
3697 1581 976 33
BstBI TT'CG_AA
Cuts at: 4648 4648
Size: 6287
BstEII G'GTnAC_C
Cuts at: 622 622
Size: 6287
BstXI CCAn_nnnn'nTGG
Cuts at: 1050 1329 1914 1050
Size: 279 585 5423
Fragments arranged by size:
5423 585 279
BstYI r'GATC_y
Cuts at: 1 263 782 794 1532 2123 2286 2483 3312
Size: 262 519 12 738 591 163 197 829
Cuts at: 3312 3779 3802 4137 4383 5395 5481 5493 5839
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5839 1
Size: 449
Fragments arranged by size:
1012 829 738 591 519 467 449 346
335 262 246 197 163 86 23 12
12
Bsu36I CC'TnA_GG
Cuts at: 191 1267 1870 2422 2636 2705 2854 191
Size: 1076 603 552 214 69 149 3624
Fragments arranged by size:
3624 1076 603 552 214 149 69
BtgI C'CryG_G
Cuts at: 107 768 917 1328 1751 107
Size: 661 149 411 423 4643
Fragments arranged by size:
4643 661 423 411 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4237 4418 4581 4237
Size: 181 163 5943
Fragments arranged by size:
5943 181 163
BtsI GCAGTG_nn'
Cuts at: 1853 3530 1853
Size: 1677 4610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1724 1759 2460 2495 1724
Size: 35 701 35 5516
Fragments arranged by size:
5516 701 35 35
DraI TTT'AAA
Cuts at: 3286 3286
Size: 6287
DraIII CAC_nnn'GTG
Cuts at: 354 354
Size: 6287
DrdI GACnn_nn'nnGTC
Cuts at: 3993 4851 3993
Size: 858 5429
EaeI y'GGCC_r
Cuts at: 170 755 827 914 1022 1331 3077 3872 4046
Size: 585 72 87 108 309 1746 795 174
Cuts at: 4046 4437 4464 5809 170
Size: 391 27 1345 648
Fragments arranged by size:
1746 1345 795 648 585 391 309 174
108 87 72 27
EagI C'GGCC_G
Cuts at: 3872 3872
Size: 6287
EarI CTCTTCn'nnn_
Cuts at: 448 1453 2045 2096 2206 2425 2854 4310 4520
Size: 1005 592 51 110 219 429 1456 210
Cuts at: 4520 448
Size: 2215
Fragments arranged by size:
2215 1456 1005 592 429 219 210 110
51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1261 4495 4805 4951 6097 1261
Size: 3234 310 146 1146 1451
Fragments arranged by size:
3234 1451 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1986 2310 3299 1986
Size: 324 989 4974
Fragments arranged by size:
4974 989 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1155 1309 2083 2158 2187 2214 2326 2449 2565
Size: 154 774 75 29 27 112 123 116
Cuts at: 2565 2767 2833 3169 3260 4112 4544 5291 5559
Size: 202 66 336 91 852 432 747 268
Cuts at: 5559 1155
Size: 1883
Fragments arranged by size:
1883 852 774 747 432 336 268 202
154 123 116 112 91 75 66 29
27
EcoNI CCTnn'n_nnAGG
Cuts at: 1213 2906 3022 1213
Size: 1693 116 4478
Fragments arranged by size:
4478 1693 116
EcoO109I rG'GnC_Cy
Cuts at: 118 633 1298 1442 1443 1635 1891 3015 3426
Size: 515 665 144 1 192 256 1124 411
Cuts at: 3426 118
Size: 2979
Fragments arranged by size:
2979 1124 665 515 411 256 192 144
1
EcoRI G'AATT_C
Cuts at: 3291 3291
Size: 6287
EcoRV GAT'ATC
Cuts at: 1311 1311
Size: 6287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1419 2454 4306 1419
Size: 1035 1852 3400
Fragments arranged by size:
3400 1852 1035
FspI TGC'GCA
Cuts at: 4068 4068
Size: 6287
HaeII r_GCGC'y
Cuts at: 537 973 2721 3207 3252 3721 3969 4991 5970
Size: 436 1748 486 45 469 248 1022 979
Cuts at: 5970 537
Size: 854
Fragments arranged by size:
1748 1022 979 854 486 469 436 248
45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 227 259 467 499 774 806 2311 2343 2592
Size: 32 208 32 275 32 1505 32 249
Cuts at: 2592 2624 4129 4159 4161 4191 227
Size: 32 1505 30 2 30 2323
Fragments arranged by size:
2323 1505 1505 275 249 208 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 558 3326 558
Size: 2768 3519
HindIII A'AGCT_T
Cuts at: 467 2491 467
Size: 2024 4263
KasI G'GCGC_C
Cuts at: 3248 3717 3965 3248
Size: 469 248 5570
Fragments arranged by size:
5570 469 248
KpnI G_GTAC'C
Cuts at: 3307 3307
Size: 6287
MluI A'CGCG_T
Cuts at: 731 2150 5720 731
Size: 1419 3570 1298
Fragments arranged by size:
3570 1419 1298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 213 389 772 4958 5142 213
Size: 176 383 4186 184 1358
Fragments arranged by size:
4186 1358 383 184 176
MscI TGG'CCA
Cuts at: 757 829 916 1024 3079 4048 757
Size: 72 87 108 2055 969 2996
Fragments arranged by size:
2996 2055 969 108 87 72
MslI CAynn'nnrTG
Cuts at: 1048 1327 1912 3049 3079 4276 4403 1048
Size: 279 585 1137 30 1197 127 2932
Fragments arranged by size:
2932 1197 1137 585 279 127 30
MspA1I CmG'CkG
Cuts at: 180 770 1507 2585 2730 2986 3398 3712 4072
Size: 590 737 1078 145 256 412 314 360
Cuts at: 4072 5085 5330 180
Size: 1013 245 1137
Fragments arranged by size:
1137 1078 1013 737 590 412 360 314
256 245 145
NaeI GCC'GGC
Cuts at: 4468 4468
Size: 6287
NarI GG'CG_CC
Cuts at: 3249 3718 3966 3249
Size: 469 248 5570
Fragments arranged by size:
5570 469 248
NcoI C'CATG_G
Cuts at: 107 1328 107
Size: 1221 5066
NdeI CA'TA_TG
Cuts at: 1361 1361
Size: 6287
NgoMIV G'CCGG_C
Cuts at: 4466 4466
Size: 6287
NspI r_CATG'y
Cuts at: 550 846 3340 3840 4747 550
Size: 296 2494 500 907 2090
Fragments arranged by size:
2494 2090 907 500 296
PasI CC'CwG_GG
Cuts at: 2527 2527
Size: 6287
PciI A'CATG_T
Cuts at: 546 4743 546
Size: 4197 2090
PflMI CCAn_nnn'nTGG
Cuts at: 2220 2304 2220
Size: 84 6203
PfoI T'CCnGG_A
Cuts at: 587 1556 2303 2957 587
Size: 969 747 654 3917
Fragments arranged by size:
3917 969 747 654
PmeI GTTT'AAAC
Cuts at: 3286 3286
Size: 6287
PmlI CAC'GTG
Cuts at: 2922 2922
Size: 6287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 166 198 5426 5458 166
Size: 32 5228 32 995
Fragments arranged by size:
5228 995 32 32
PpuMI rG'GwC_Cy
Cuts at: 118 633 1891 3015 118
Size: 515 1258 1124 3390
Fragments arranged by size:
3390 1258 1124 515
PshAI GACnn'nnGTC
Cuts at: 2114 2114
Size: 6287
PspOMI G'GGCC_C
Cuts at: 801 1442 801
Size: 641 5646
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1602 1634 1602
Size: 32 6255
PstI C_TGCA'G
Cuts at: 279 344 2878 3334 4019 279
Size: 65 2534 456 685 2547
Fragments arranged by size:
2547 2534 685 456 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6287
PvuII CAG'CTG
Cuts at: 180 1507 2730 2986 3712 4072 180
Size: 1327 1223 256 726 360 2395
Fragments arranged by size:
2395 1327 1223 726 360 256
RsrII CG'GwC_CG
Cuts at: 4482 4482
Size: 6287
SacI G_AGCT'C
Cuts at: 1988 2312 3301 1988
Size: 324 989 4974
Fragments arranged by size:
4974 989 324
SacII CC_GC'GG
Cuts at: 771 771
Size: 6287
SalI G'TCGA_C
Cuts at: 3324 3324
Size: 6287
SanDI GG'GwC_CC
Cuts at: 1891 1891
Size: 6287
SapI GCTCTTCn'nnn_
Cuts at: 2045 2425 4310 4520 2045
Size: 380 1885 210 3812
Fragments arranged by size:
3812 1885 380 210
SbfI CC_TGCA'GG
Cuts at: 3334 3334
Size: 6287
ScaI AGT'ACT
Cuts at: 5804 5804
Size: 6287
SfcI C'TryA_G
Cuts at: 33 275 340 2874 3330 4015 5008 5199 33
Size: 242 65 2534 456 685 993 191 1121
Fragments arranged by size:
2534 1121 993 685 456 242 191 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 1753 1753
Size: 6287
SfoI GGC'GCC
Cuts at: 3250 3719 3967 3250
Size: 469 248 5570
Fragments arranged by size:
5570 469 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
SmaI CCC'GGG
Cuts at: 806 3309 806
Size: 2503 3784
SmlI C'TyrA_G
Cuts at: 255 2044 2085 3442 4357 4849 5111 255
Size: 1789 41 1357 915 492 262 1431
Fragments arranged by size:
1789 1431 1357 915 492 262 41
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6287
SphI G_CATG'C
Cuts at: 3340 3840 3340
Size: 500 5787
StuI AGG'CCT
Cuts at: 253 253
Size: 6287
StyI C'CwwG_G
Cuts at: 107 114 332 1214 1301 1328 1961 2019 2252
Size: 7 218 882 87 27 633 58 233
Cuts at: 2252 2692 2992 3122 3421 3775 107
Size: 440 300 130 299 354 2619
Fragments arranged by size:
2619 882 633 440 354 300 299 233
218 130 87 58 27 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 961 1843 1974 2540 3061 3870 4003 4462 4671
Size: 882 131 566 521 809 133 459 209
Cuts at: 4671 961
Size: 2577
Fragments arranged by size:
2577 882 809 566 521 459 209 133
131
TatI w'GTAC_w
Cuts at: 5802 5802
Size: 6287
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 583 615 804 836 936 968 2248 2280 2299
Size: 32 189 32 100 32 1280 32 19
Cuts at: 2299 2331 5804 5836 583
Size: 32 3473 32 1034
Fragments arranged by size:
3473 1280 1034 189 100 32 32 32
32 32 32 19
Tth111I GACn'n_nGTC
Cuts at: 360 2965 4084 360
Size: 2605 1119 2563
Fragments arranged by size:
2605 2563 1119
XbaI T'CTAG_A
Cuts at: 2139 3318 2139
Size: 1179 5108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 750 2742 3782 750
Size: 1992 1040 3255
Fragments arranged by size:
3255 1992 1040
XmaI C'CCGG_G
Cuts at: 804 3307 804
Size: 2503 3784
XmnI GAAnn'nnTTC
Cuts at: 2434 2786 2434
Size: 352 5935
ZraI GAC'GTC
Cuts at: 594 1497 594
Size: 903 5384
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AscI BaeI BfrBI BmtI BsiWI BsmI BsrGI
BssHII BstZ17I ClaI FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PsiI PspXI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI