Restriction map for
pF1KB2260
(Circular) (Six-base) MAPSORT of: pF1KB2260.seq Check: 1966 from: 1 to: 5741
pF1KB2260 5741 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 360 2586 360
Size: 2226 3515
AccI GT'mk_AC
Cuts at: 1883 2779 1883
Size: 896 4845
Acc65I G'GTAC_C
Cuts at: 2757 2757
Size: 5741
AclI AA'CG_TT
Cuts at: 5581 5581
Size: 5741
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 1167 1275 3566 3998 4351 5013 583
Size: 584 108 2291 432 353 662 1311
Fragments arranged by size:
2291 1311 662 584 432 353 108
AflIII A'CryG_T
Cuts at: 2471 3093 4893 5174 2471
Size: 622 1800 281 3038
Fragments arranged by size:
3038 1800 622 281
AleI CACnn'nnGTG
Cuts at: 1874 1874
Size: 5741
AlwNI CAG_nnn'CTG
Cuts at: 263 846 3046 4484 263
Size: 583 2200 1438 1520
Fragments arranged by size:
2200 1520 1438 583
ApaI G_GGCC'C
Cuts at: 578 1172 1796 2175 2204 2523 578
Size: 594 624 379 29 319 3796
Fragments arranged by size:
3796 624 594 379 319 29
ApaLI G'TGCA_C
Cuts at: 1867 4579 1867
Size: 2712 3029
ApoI r'AATT_y
Cuts at: 2325 2745 5094 5350 2325
Size: 420 2349 256 2716
Fragments arranged by size:
2716 2349 420 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5741
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5741
AvaI C'yCGr_G
Cuts at: 476 611 1100 2761 476
Size: 135 489 1661 3456
Fragments arranged by size:
3456 1661 489 135
AvrII C'CTAG_G
Cuts at: 1071 1071
Size: 5741
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1540 1573 1540
Size: 33 5708
BamHI G'GATC_C
Cuts at: 2766 2766
Size: 5741
BanI G'GyrC_C
Cuts at: 326 409 1240 1657 2757 3171 3419 3454 326
Size: 83 831 417 1100 414 248 35 2613
Fragments arranged by size:
2613 1100 831 417 414 248 83 35
BanII G_rGCy'C
Cuts at: 578 653 993 1058 1101 1172 1313 1351 1568
Size: 75 340 65 43 71 141 38 217
Cuts at: 1568 1796 2175 2204 2523 2755 3785 578
Size: 228 379 29 319 232 1030 2534
Fragments arranged by size:
2534 1030 379 340 319 232 228 217
141 75 71 65 43 38 29
BbeI G_GCGC'C
Cuts at: 1661 3175 3423 1661
Size: 1514 248 3979
Fragments arranged by size:
3979 1514 248
BbvCI CC'TCA_GC
Cuts at: 937 1447 937
Size: 510 5231
BciVI GTATCCnnnnn_n'
Cuts at: 3639 4695 5741 3639
Size: 1056 1046 3639
Fragments arranged by size:
3639 1056 1046
BclI T'GATC_A
Cuts at: 3261 3261
Size: 5741
BglI GCCn_nnn'nGGC
Cuts at: 949 2170 2532 2630 949
Size: 1221 362 98 4060
Fragments arranged by size:
4060 1221 362 98
BglII A'GATC_T
Cuts at: 1896 1961 3256 5293 1896
Size: 65 1295 2037 2344
Fragments arranged by size:
2344 2037 1295 65
BlpI GC'TnA_GC
Cuts at: 2853 2853
Size: 5741
Bme1580I G_kGCm'C
Cuts at: 331 412 578 1118 1172 1796 1871 2142 2175
Size: 81 166 540 54 624 75 271 33
Cuts at: 2175 2204 2523 3366 3459 4583 331
Size: 29 319 843 93 1124 1489
Fragments arranged by size:
1489 1124 843 624 540 319 271 166
93 81 75 54 33 29
BmgBI CAC'GTC
Cuts at: 2472 2472
Size: 5741
BmrI ACTGGGnnnn_n'
Cuts at: 1243 2953 3034 3369 5513 1243
Size: 1710 81 335 2144 1471
Fragments arranged by size:
2144 1710 1471 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 546 4108 546
Size: 3562 2179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 882 1327 2170 2679 687
Size: 195 445 843 509 3749
Fragments arranged by size:
3749 843 509 445 195
Bpu10I CC'TnA_GC
Cuts at: 937 1447 937
Size: 510 5231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2017 2917 3796 4510 4808 1877
Size: 140 900 879 714 298 2810
Fragments arranged by size:
2810 900 879 714 298 140
BsaI GGTCTCn'nnnn_
Cuts at: 5445 5445
Size: 5741
BsaAI yAC'GTr
Cuts at: 3094 3094
Size: 5741
BsaBI GATnn'nnATC
Cuts at: 6 3255 5292 6
Size: 3249 2037 455
Fragments arranged by size:
3249 2037 455
BsaHI Gr'CG_yC
Cuts at: 1658 1733 3172 3420 4122 5618 1658
Size: 75 1439 248 702 1496 1781
Fragments arranged by size:
1781 1496 1439 702 248 75
BsaWI w'CCGG_w
Cuts at: 224 2930 3451 4129 4540 4687 4961 224
Size: 2706 521 678 411 147 274 1004
Fragments arranged by size:
2706 1004 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 346 376 880 904 910 934 940 346
Size: 30 504 24 6 24 6 5147
Fragments arranged by size:
5147 504 30 24 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 373 1435 1492 370
Size: 3 1062 57 4619
Fragments arranged by size:
4619 1062 57 3
BseYI C'CCAG_C
Cuts at: 512 984 2058 2374 2522 2693 3166 3924 4589
Size: 472 1074 316 148 171 473 758 665
Cuts at: 4589 4997 512
Size: 408 1256
Fragments arranged by size:
1256 1074 758 665 473 472 408 316
171 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1414 1748 2092 2413 373
Size: 1041 334 344 321 3701
Fragments arranged by size:
3701 1041 344 334 321
BsiEI CG_ry'CG
Cuts at: 104 1128 1355 2614 3283 3329 4559 104
Size: 1024 227 1259 669 46 1230 1286
Fragments arranged by size:
1286 1259 1230 1024 669 227 46
BsiHKAI G_wGCw'C
Cuts at: 653 1871 2755 3533 3723 4583 653
Size: 1218 884 778 190 860 1811
Fragments arranged by size:
1811 1218 884 860 778 190
BsmI GAATG_Cn'
Cuts at: 1992 1992
Size: 5741
Bsp1286I G_dGCh'C
Cuts at: 331 412 578 653 993 1058 1101 1118 1172
Size: 81 166 75 340 65 43 17 54
Cuts at: 1172 1313 1351 1568 1796 1871 2142 2175 2204
Size: 141 38 217 228 75 271 33 29
Cuts at: 2204 2523 2755 3366 3459 3533 3723 3785 4583
Size: 319 232 611 93 74 190 62 798
Cuts at: 4583 331
Size: 1489
Fragments arranged by size:
1489 798 611 340 319 271 232 228
217 190 166 141 93 81 75 75
74 65 62 54 43 38 33 29
17
BspHI T'CATG_A
Cuts at: 626 4173 626
Size: 3547 2194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 284 360 948 2541 2586 2791 3005 3307 3688
Size: 76 588 1593 45 205 214 302 381
Cuts at: 3688 284
Size: 2337
Fragments arranged by size:
2337 1593 588 381 302 214 205 76
45
BsrBI CCG'CTC
Cuts at: 50 4033 4087 5571 5736 50
Size: 3983 54 1484 165 55
Fragments arranged by size:
3983 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 543 1406 3653 543
Size: 863 2247 2631
Fragments arranged by size:
2631 2247 863
BsrFI r'CCGG_y
Cuts at: 461 932 2591 3739 3920 461
Size: 471 1659 1148 181 2282
Fragments arranged by size:
2282 1659 1148 471 181
BsrGI T'GTAC_A
Cuts at: 2283 2283
Size: 5741
BssSI C'ACGA_G
Cuts at: 1574 4012 4720 1574
Size: 2438 708 2595
Fragments arranged by size:
2595 2438 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2719 3118 2719
Size: 399 5342
BstBI TT'CG_AA
Cuts at: 4102 4102
Size: 5741
BstXI CCAn_nnnn'nTGG
Cuts at: 1259 2530 1259
Size: 1271 4470
BstYI r'GATC_y
Cuts at: 1 375 1896 1961 2766 3233 3256 3591 3837
Size: 374 1521 65 805 467 23 335 246
Cuts at: 3837 4143 4155 4241 5293 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1521 1052 805 467 449 374 335 306
246 86 65 23 12
Bsu36I CC'TnA_GG
Cuts at: 548 548
Size: 5741
BtgI C'CryG_G
Cuts at: 107 581 764 1037 1328 107
Size: 474 183 273 291 4520
Fragments arranged by size:
4520 474 291 273 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3691 3872 4035 3691
Size: 181 163 5397
Fragments arranged by size:
5397 181 163
BtsI GCAGTG_nn'
Cuts at: 275 312 1153 1765 2411 2984 275
Size: 37 841 612 646 573 3032
Fragments arranged by size:
3032 841 646 612 573 37
DraI TTT'AAA
Cuts at: 2740 2740
Size: 5741
DraIII CAC_nnn'GTG
Cuts at: 396 396
Size: 5741
DrdI GACnn_nn'nnGTC
Cuts at: 2551 3447 4791 2551
Size: 896 1344 3501
Fragments arranged by size:
3501 1344 896
EaeI y'GGCC_r
Cuts at: 856 1125 1264 1331 1379 1969 2267 2297 2611
Size: 269 139 67 48 590 298 30 314
Cuts at: 2611 3326 3500 3891 3918 5263 856
Size: 715 174 391 27 1345 1334
Fragments arranged by size:
1345 1334 715 590 391 314 298 269
174 139 67 48 30 27
EagI C'GGCC_G
Cuts at: 1125 2611 3326 1125
Size: 1486 715 3540
Fragments arranged by size:
3540 1486 715
EarI CTCTTCn'nnn_
Cuts at: 829 1186 1469 3764 3974 829
Size: 357 283 2295 210 2596
Fragments arranged by size:
2596 2295 357 283 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 785 1574 3949 4691 4837 5551 785
Size: 789 2375 742 146 714 975
Fragments arranged by size:
2375 975 789 742 714 146
EcoICRI GAG'CTC
Cuts at: 651 2753 651
Size: 2102 3639
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 687 882 1167 1275 1327 2170 2679 3566
Size: 104 195 285 108 52 843 509 887
Cuts at: 3566 3998 4351 5013 583
Size: 432 353 662 1311
Fragments arranged by size:
1311 887 843 662 509 432 353 285
195 108 104 52
EcoNI CCTnn'n_nnAGG
Cuts at: 439 1450 1876 2008 2497 2713 439
Size: 1011 426 132 489 216 3467
Fragments arranged by size:
3467 1011 489 426 216 132
EcoO109I rG'GnC_Cy
Cuts at: 120 320 432 543 567 575 941 1089 1168
Size: 200 112 111 24 8 366 148 79
Cuts at: 1168 1169 1537 1793 2037 2171 2172 2200 2292
Size: 1 368 256 244 134 1 28 92
Cuts at: 2292 2519 2631 2880 120
Size: 227 112 249 2981
Fragments arranged by size:
2981 368 366 256 249 244 227 200
148 134 112 112 111 92 79 28
24 8 1 1
EcoRI G'AATT_C
Cuts at: 2745 2745
Size: 5741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3760 3760
Size: 5741
FspI TGC'GCA
Cuts at: 2195 3522 2195
Size: 1327 4414
HaeII r_GCGC'y
Cuts at: 1661 3175 3423 4653 5424 1661
Size: 1514 248 1230 771 1978
Fragments arranged by size:
1978 1514 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 621 653 1808 1840 3583 3613 3615
Size: 32 480 32 1155 32 1743 30 2
Cuts at: 3615 3645 109
Size: 30 2205
Fragments arranged by size:
2205 1743 1155 480 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1884 2780 1884
Size: 896 4845
HindIII A'AGCT_T
Cuts at: 1840 1840
Size: 5741
KasI G'GCGC_C
Cuts at: 1657 3171 3419 1657
Size: 1514 248 3979
Fragments arranged by size:
3979 1514 248
KpnI G_GTAC'C
Cuts at: 2761 2761
Size: 5741
MluI A'CGCG_T
Cuts at: 5174 5174
Size: 5741
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 278 1451 4500 4684 278
Size: 1173 3049 184 1335
Fragments arranged by size:
3049 1335 1173 184
MscI TGG'CCA
Cuts at: 858 1333 1381 2269 2299 3502 858
Size: 475 48 888 30 1203 3097
Fragments arranged by size:
3097 1203 888 475 48 30
MslI CAynn'nnrTG
Cuts at: 1874 2443 3730 3857 1874
Size: 569 1287 127 3758
Fragments arranged by size:
3758 1287 569 127
MspA1I CmG'CkG
Cuts at: 491 512 798 2148 2420 2697 2852 3166 3526
Size: 21 286 1350 272 277 155 314 360
Cuts at: 3526 4310 4555 491
Size: 784 245 1677
Fragments arranged by size:
1677 1350 784 360 314 286 277 272
245 155 21
NaeI GCC'GGC
Cuts at: 2593 3922 2593
Size: 1329 4412
NarI GG'CG_CC
Cuts at: 1658 3172 3420 1658
Size: 1514 248 3979
Fragments arranged by size:
3979 1514 248
NcoI C'CATG_G
Cuts at: 107 581 1037 107
Size: 474 456 4811
Fragments arranged by size:
4811 474 456
NgoMIV G'CCGG_C
Cuts at: 2591 3920 2591
Size: 1329 4412
NspI r_CATG'y
Cuts at: 219 427 978 2233 2259 2794 3294 4897 219
Size: 208 551 1255 26 535 500 1603 1063
Fragments arranged by size:
1603 1255 1063 551 535 500 208 26
PasI CC'CwG_GG
Cuts at: 210 414 1504 1774 2176 210
Size: 204 1090 270 402 3775
Fragments arranged by size:
3775 1090 402 270 204
PciI A'CATG_T
Cuts at: 4893 4893
Size: 5741
PflMI CCAn_nnn'nTGG
Cuts at: 263 1043 2148 2420 263
Size: 780 1105 272 3584
Fragments arranged by size:
3584 1105 780 272
PfoI T'CCnGG_A
Cuts at: 451 2579 451
Size: 2128 3613
PmeI GTTT'AAAC
Cuts at: 2740 2740
Size: 5741
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1207 1239 4187 4219 1207
Size: 32 2948 32 2729
Fragments arranged by size:
2948 2729 32 32
PpuMI rG'GwC_Cy
Cuts at: 543 567 1089 1537 2037 2292 543
Size: 24 522 448 500 255 3992
Fragments arranged by size:
3992 522 500 448 255 24
PshAI GACnn'nnGTC
Cuts at: 681 681
Size: 5741
PspOMI G'GGCC_C
Cuts at: 574 1168 1792 2171 2200 2519 574
Size: 594 624 379 29 319 3796
Fragments arranged by size:
3796 624 594 379 319 29
PstI C_TGCA'G
Cuts at: 779 1148 1730 2788 3473 779
Size: 369 582 1058 685 3047
Fragments arranged by size:
3047 1058 685 582 369
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5741
PvuII CAG'CTG
Cuts at: 798 2148 2697 3166 3526 798
Size: 1350 549 469 360 3013
Fragments arranged by size:
3013 1350 549 469 360
RsrII CG'GwC_CG
Cuts at: 3936 3936
Size: 5741
SacI G_AGCT'C
Cuts at: 653 2755 653
Size: 2102 3639
SalI G'TCGA_C
Cuts at: 1882 2778 1882
Size: 896 4845
SanDI GG'GwC_CC
Cuts at: 1089 1089
Size: 5741
SapI GCTCTTCn'nnn_
Cuts at: 3764 3974 3764
Size: 210 5531
SbfI CC_TGCA'GG
Cuts at: 1730 2788 1730
Size: 1058 4683
ScaI AGT'ACT
Cuts at: 364 5258 364
Size: 4894 847
SexAI A'CCwGG_T
Cuts at: 392 392
Size: 5741
SfcI C'TryA_G
Cuts at: 33 775 1144 1623 1726 2514 2784 3469 4437
Size: 742 369 479 103 788 270 685 968
Cuts at: 4437 4628 33
Size: 191 1146
Fragments arranged by size:
1146 968 788 742 685 479 369 270
191 103
SfoI GGC'GCC
Cuts at: 1659 3173 3421 1659
Size: 1514 248 3979
Fragments arranged by size:
3979 1514 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5741
SmaI CCC'GGG
Cuts at: 478 613 1102 2763 478
Size: 135 489 1661 3456
Fragments arranged by size:
3456 1661 489 135
SmlI C'TyrA_G
Cuts at: 1892 2032 2896 3811 4525 4787 1892
Size: 140 864 915 714 262 2846
Fragments arranged by size:
2846 915 864 714 262 140
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5741
SphI G_CATG'C
Cuts at: 219 427 2259 2794 3294 219
Size: 208 1832 535 500 2666
Fragments arranged by size:
2666 1832 535 500 208
SrfI GCCC'GGGC
Cuts at: 1102 1102
Size: 5741
StyI C'CwwG_G
Cuts at: 107 350 570 581 1037 1071 1119 1334 2875
Size: 243 220 11 456 34 48 215 1541
Cuts at: 2875 3229 107
Size: 354 2619
Fragments arranged by size:
2619 1541 456 354 243 220 215 48
34 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 427 3324 3457 3916 4125 427
Size: 2897 133 459 209 2043
Fragments arranged by size:
2897 2043 459 209 133
TatI w'GTAC_w
Cuts at: 362 2283 5256 362
Size: 1921 2973 847
Fragments arranged by size:
2973 1921 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 749 781 880 912 5258 5290 749
Size: 32 99 32 4346 32 1200
Fragments arranged by size:
4346 1200 99 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 622 3538 622
Size: 2916 2825
XbaI T'CTAG_A
Cuts at: 2772 2772
Size: 5741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 802 906 1259 2421 3236 802
Size: 104 353 1162 815 3307
Fragments arranged by size:
3307 1162 815 353 104
XmaI C'CCGG_G
Cuts at: 476 611 1100 2761 476
Size: 135 489 1661 3456
Fragments arranged by size:
3456 1661 489 135
XmnI GAAnn'nnTTC
Cuts at: 1999 1999
Size: 5741
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
BbsI BcgI BfrBI BmtI BsiWI BsmBI BspEI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SspI
StuI SwaI XhoI ZraI