Restriction map for
pF1KB1891
(Circular) (Six-base) MAPSORT of: pF1KB1891.seq Check: 2193 from: 1 to: 7502
pF1KB1891 7502 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1075 1388 2716 4418 4477 4540 1075
Size: 313 1328 1702 59 63 4037
Fragments arranged by size:
4037 1702 1328 313 63 59
Acc65I G'GTAC_C
Cuts at: 4518 4518
Size: 7502
AclI AA'CG_TT
Cuts at: 7342 7342
Size: 7502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 1172 2048 2321 3599 5327 5759 6112 6774
Size: 524 876 273 1278 1728 432 353 662
Cuts at: 6774 648
Size: 1376
Fragments arranged by size:
1728 1376 1278 876 662 524 432 353
273
AflIII A'CryG_T
Cuts at: 1078 1762 2050 4854 6654 6935 1078
Size: 684 288 2804 1800 281 1645
Fragments arranged by size:
2804 1800 1645 684 288 281
AleI CACnn'nnGTG
Cuts at: 3886 3886
Size: 7502
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 900 932 3204 3236 900
Size: 32 2272 32 5166
Fragments arranged by size:
5166 2272 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1345 4807 6245 1345
Size: 3462 1438 2602
Fragments arranged by size:
3462 2602 1438
ApaLI G'TGCA_C
Cuts at: 6340 6340
Size: 7502
ApoI r'AATT_y
Cuts at: 376 486 669 745 2346 3138 3267 3276 3423
Size: 110 183 76 1601 792 129 9 147
Cuts at: 3423 3609 4241 4380 4506 6855 7111 376
Size: 186 632 139 126 2349 256 767
Fragments arranged by size:
2349 1601 792 767 632 256 186 183
147 139 129 126 110 76 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 7502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7502
AvaI C'yCGr_G
Cuts at: 1591 4522 1591
Size: 2931 4571
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3905 3938 3905
Size: 33 7469
BamHI G'GATC_C
Cuts at: 4527 4527
Size: 7502
BanI G'GyrC_C
Cuts at: 4518 4932 5180 5215 4518
Size: 414 248 35 6805
Fragments arranged by size:
6805 414 248 35
BanII G_rGCy'C
Cuts at: 1285 1933 1996 4516 5546 1285
Size: 648 63 2520 1030 3241
Fragments arranged by size:
3241 2520 1030 648 63
BbeI G_GCGC'C
Cuts at: 4936 5184 4936
Size: 248 7254
BbsI GAAGACnn'nnnn_
Cuts at: 164 285 2375 4228 164
Size: 121 2090 1853 3438
Fragments arranged by size:
3438 2090 1853 121
BbvCI CC'TCA_GC
Cuts at: 3860 3860
Size: 7502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4199 4233 4199
Size: 34 7468
BciVI GTATCCnnnnn_n'
Cuts at: 557 5400 6456 7502 557
Size: 4843 1056 1046 557
Fragments arranged by size:
4843 1056 1046 557
BclI T'GATC_A
Cuts at: 5022 5022
Size: 7502
BfrBI ATG'CAT
Cuts at: 970 970
Size: 7502
BglII A'GATC_T
Cuts at: 528 1548 2185 2479 2737 2785 2914 3523 5017
Size: 1020 637 294 258 48 129 609 1494
Cuts at: 5017 7054 528
Size: 2037 976
Fragments arranged by size:
2037 1494 1020 976 637 609 294 258
129 48
BlpI GC'TnA_GC
Cuts at: 4614 4614
Size: 7502
Bme1580I G_kGCm'C
Cuts at: 3893 5127 5220 6344 3893
Size: 1234 93 1124 5051
Fragments arranged by size:
5051 1234 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2544 2585 4714 4795 5130 7274 2544
Size: 41 2129 81 335 2144 2772
Fragments arranged by size:
2772 2144 2129 335 81 41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 3910 5869 2708
Size: 1202 1959 4341
Fragments arranged by size:
4341 1959 1202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 728
Size: 7502
Bpu10I CC'TnA_GC
Cuts at: 3860 3860
Size: 7502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 968 1529 2919 4678 5557 6271 6569 968
Size: 561 1390 1759 879 714 298 1901
Fragments arranged by size:
1901 1759 1390 879 714 561 298
BsaI GGTCTCn'nnnn_
Cuts at: 477 2708 7206 477
Size: 2231 4498 773
Fragments arranged by size:
4498 2231 773
BsaAI yAC'GTr
Cuts at: 1081 4855 1081
Size: 3774 3728
BsaBI GATnn'nnATC
Cuts at: 6 1524 2742 3534 3829 5016 7053 6
Size: 1518 1218 792 295 1187 2037 455
Fragments arranged by size:
2037 1518 1218 1187 792 455 295
BsaHI Gr'CG_yC
Cuts at: 4933 5181 5883 7379 4933
Size: 248 702 1496 5056
Fragments arranged by size:
5056 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4691 5212 5890 6301 6448 6722 4691
Size: 521 678 411 147 274 5471
Fragments arranged by size:
5471 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1930 2886 3661 1930
Size: 956 775 5771
Fragments arranged by size:
5771 956 775
BseYI C'CCAG_C
Cuts at: 3548 4927 5685 6350 6758 3548
Size: 1379 758 665 408 4292
Fragments arranged by size:
4292 1379 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 1432
Size: 7502
BsiEI CG_ry'CG
Cuts at: 104 5044 5090 6320 104
Size: 4940 46 1230 1286
Fragments arranged by size:
4940 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1285 1376 1545 4516 5294 5484 6344 1285
Size: 91 169 2971 778 190 860 2443
Fragments arranged by size:
2971 2443 860 778 190 169 91
BsmI GAATG_Cn'
Cuts at: 3771 3771
Size: 7502
Bsp1286I G_dGCh'C
Cuts at: 1285 1376 1545 1933 1996 3893 4516 5127 5220
Size: 91 169 388 63 1897 623 611 93
Cuts at: 5220 5294 5484 5546 6344 1285
Size: 74 190 62 798 2443
Fragments arranged by size:
2443 1897 798 623 611 388 190 169
93 91 74 63 62
BspHI T'CATG_A
Cuts at: 480 5934 480
Size: 5454 2048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1056 3891 4552 4766 5068 5449 1056
Size: 2835 661 214 302 381 3109
Fragments arranged by size:
3109 2835 661 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5794 5848 7332 7497 50
Size: 5744 54 1484 165 55
Fragments arranged by size:
5744 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1058 1464 3980 5414 1058
Size: 406 2516 1434 3146
Fragments arranged by size:
3146 2516 1434 406
BsrFI r'CCGG_y
Cuts at: 5500 5681 5500
Size: 181 7321
BsrGI T'GTAC_A
Cuts at: 209 739 1732 3579 209
Size: 530 993 1847 4132
Fragments arranged by size:
4132 1847 993 530
BssSI C'ACGA_G
Cuts at: 3041 5773 6481 3041
Size: 2732 708 4062
Fragments arranged by size:
4062 2732 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4879 4879
Size: 7502
BstBI TT'CG_AA
Cuts at: 938 5863 938
Size: 4925 2577
BstEII G'GTnAC_C
Cuts at: 2510 2510
Size: 7502
BstXI CCAn_nnnn'nTGG
Cuts at: 1696 1696
Size: 7502
BstYI r'GATC_y
Cuts at: 1 528 1117 1519 1548 2185 2479 2737 2785
Size: 527 589 402 29 637 294 258 48
Cuts at: 2785 2914 3523 4527 4994 5017 5352 5598 5904
Size: 129 609 1004 467 23 335 246 306
Cuts at: 5904 5916 6002 7054 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 1004 637 609 589 527 467 449
402 335 306 294 258 246 129 86
48 29 23 12
BstZ17I GTA'TAC
Cuts at: 2717 2717
Size: 7502
BtgI C'CryG_G
Cuts at: 3318 3339 3318
Size: 21 7481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5452 5633 5796 5452
Size: 181 163 7158
Fragments arranged by size:
7158 181 163
BtsI GCAGTG_nn'
Cuts at: 374 2289 4202 4745 374
Size: 1915 1913 543 3131
Fragments arranged by size:
3131 1915 1913 543
DraI TTT'AAA
Cuts at: 375 4501 375
Size: 4126 3376
DrdI GACnn_nn'nnGTC
Cuts at: 5208 6552 5208
Size: 1344 6158
EaeI y'GGCC_r
Cuts at: 4274 5087 5261 5652 5679 7024 4274
Size: 813 174 391 27 1345 4752
Fragments arranged by size:
4752 1345 813 391 174 27
EagI C'GGCC_G
Cuts at: 5087 5087
Size: 7502
EarI CTCTTCn'nnn_
Cuts at: 781 1191 1236 2562 2754 5525 5735 781
Size: 410 45 1326 192 2771 210 2548
Fragments arranged by size:
2771 2548 1326 410 210 192 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5710 6452 6598 7312 5710
Size: 742 146 714 5900
Fragments arranged by size:
5900 742 714 146
EcoICRI GAG'CTC
Cuts at: 1283 4514 1283
Size: 3231 4271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 728 1172 2048 2321 3599 5327 5759 6112
Size: 80 444 876 273 1278 1728 432 353
Cuts at: 6112 6774 648
Size: 662 1376
Fragments arranged by size:
1728 1376 1278 876 662 444 432 353
273 80
EcoNI CCTnn'n_nnAGG
Cuts at: 3898 3898
Size: 7502
EcoO109I rG'GnC_Cy
Cuts at: 2831 2927 4641 2831
Size: 96 1714 5692
Fragments arranged by size:
5692 1714 96
EcoRI G'AATT_C
Cuts at: 3423 4506 3423
Size: 1083 6419
EcoRV GAT'ATC
Cuts at: 1694 4305 1694
Size: 2611 4891
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2783 5521 2783
Size: 2738 4764
FspI TGC'GCA
Cuts at: 5283 5283
Size: 7502
HaeII r_GCGC'y
Cuts at: 4936 5184 6414 7185 4936
Size: 248 1230 771 5253
Fragments arranged by size:
5253 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 639 671 1678 1710 1966 1998 2163 2195 2977
Size: 32 1007 32 256 32 165 32 782
Cuts at: 2977 3009 3640 3672 5344 5374 5376 5406 639
Size: 32 631 32 1672 30 2 30 2735
Fragments arranged by size:
2735 1672 1007 782 631 256 165 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3975 4541 3975
Size: 566 6936
KasI G'GCGC_C
Cuts at: 4932 5180 4932
Size: 248 7254
KpnI G_GTAC'C
Cuts at: 4522 4522
Size: 7502
MfeI C'AATT_G
Cuts at: 2005 3171 4006 2005
Size: 1166 835 5501
Fragments arranged by size:
5501 1166 835
MluI A'CGCG_T
Cuts at: 6935 6935
Size: 7502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 943 3010 3858 4096 6261 6445 943
Size: 2067 848 238 2165 184 2000
Fragments arranged by size:
2165 2067 2000 848 238 184
MscI TGG'CCA
Cuts at: 4276 5263 4276
Size: 987 6515
MslI CAynn'nnrTG
Cuts at: 2234 3122 3158 3886 5491 5618 2234
Size: 888 36 728 1605 127 4118
Fragments arranged by size:
4118 1605 888 728 127 36
MspA1I CmG'CkG
Cuts at: 423 1192 3548 4613 4927 5287 6071 6316 423
Size: 769 2356 1065 314 360 784 245 1609
Fragments arranged by size:
2356 1609 1065 784 769 360 314 245
NaeI GCC'GGC
Cuts at: 5683 5683
Size: 7502
NarI GG'CG_CC
Cuts at: 4933 5181 4933
Size: 248 7254
NgoMIV G'CCGG_C
Cuts at: 5681 5681
Size: 7502
NsiI A_TGCA'T
Cuts at: 972 972
Size: 7502
NspI r_CATG'y
Cuts at: 1766 2020 2054 4555 5055 6658 1766
Size: 254 34 2501 500 1603 2610
Fragments arranged by size:
2610 2501 1603 500 254 34
PciI A'CATG_T
Cuts at: 1762 2050 6654 1762
Size: 288 4604 2610
Fragments arranged by size:
4604 2610 288
PflMI CCAn_nnn'nTGG
Cuts at: 2900 3628 4117 2900
Size: 728 489 6285
Fragments arranged by size:
6285 728 489
PmeI GTTT'AAAC
Cuts at: 4501 4501
Size: 7502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5948 5980 5948
Size: 32 7470
PpuMI rG'GwC_Cy
Cuts at: 2831 2927 2831
Size: 96 7406
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1721 1753 4008 4040 1721
Size: 32 2255 32 5183
Fragments arranged by size:
5183 2255 32 32
PstI C_TGCA'G
Cuts at: 4549 5234 4549
Size: 685 6817
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7502
PvuII CAG'CTG
Cuts at: 423 1192 3548 4927 5287 423
Size: 769 2356 1379 360 2638
Fragments arranged by size:
2638 2356 1379 769 360
RsrII CG'GwC_CG
Cuts at: 5697 5697
Size: 7502
SacI G_AGCT'C
Cuts at: 1285 4516 1285
Size: 3231 4271
SalI G'TCGA_C
Cuts at: 4539 4539
Size: 7502
SapI GCTCTTCn'nnn_
Cuts at: 5525 5735 5525
Size: 210 7292
SbfI CC_TGCA'GG
Cuts at: 4549 4549
Size: 7502
ScaI AGT'ACT
Cuts at: 7019 7019
Size: 7502
SfcI C'TryA_G
Cuts at: 33 726 1286 2189 4545 5230 6198 6389 33
Size: 693 560 903 2356 685 968 191 1146
Fragments arranged by size:
2356 1146 968 903 693 685 560 191
SfoI GGC'GCC
Cuts at: 4934 5182 4934
Size: 248 7254
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7502
SmaI CCC'GGG
Cuts at: 4524 4524
Size: 7502
SmlI C'TyrA_G
Cuts at: 983 1544 1591 2934 4657 5572 6286 6548 983
Size: 561 47 1343 1723 915 714 262 1937
Fragments arranged by size:
1937 1723 1343 915 714 561 262 47
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7502
SphI G_CATG'C
Cuts at: 4555 5055 4555
Size: 500 7002
SspI AAT'ATT
Cuts at: 1650 2085 4432 1650
Size: 435 2347 4720
Fragments arranged by size:
4720 2347 435
StyI C'CwwG_G
Cuts at: 4310 4636 4990 4310
Size: 326 354 6822
Fragments arranged by size:
6822 354 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1130 2188 2529 2690 3796 5085 5218 5677 5886
Size: 1058 341 161 1106 1289 133 459 209
Cuts at: 5886 1130
Size: 2746
Fragments arranged by size:
2746 1289 1106 1058 459 341 209 161
133
TatI w'GTAC_w
Cuts at: 209 649 739 1405 1732 3579 4051 7017 209
Size: 440 90 666 327 1847 472 2966 694
Fragments arranged by size:
2966 1847 694 666 472 440 327 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7019 7051 7019
Size: 32 7470
Tth111I GACn'n_nGTC
Cuts at: 5299 5299
Size: 7502
XbaI T'CTAG_A
Cuts at: 2482 2770 3120 4533 2482
Size: 288 350 1413 5451
Fragments arranged by size:
5451 1413 350 288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2504 3629 3700 4997 2504
Size: 1125 71 1297 5009
Fragments arranged by size:
5009 1297 1125 71
XhoI C'TCGA_G
Cuts at: 1591 1591
Size: 7502
XmaI C'CCGG_G
Cuts at: 4522 4522
Size: 7502
XmnI GAAnn'nnTTC
Cuts at: 878 1649 2219 3612 3790 878
Size: 771 570 1393 178 4590
Fragments arranged by size:
4590 1393 771 570 178
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
AscI AvrII BglI BmgBI BmtI BsaXI BsiWI BsmBI
BspEI BssHII Bsu36I ClaI CspCI DraIII FseI FspAI
HindIII HpaI NcoI NdeI NheI NotI NruI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
ZraI