Restriction map for
pF1KB1630
(Circular) (Six-base) MAPSORT of: pF1KB1630.seq Check: 5434 from: 1 to: 6269
pF1KB1630 6269 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 823 1378 3307 823
Size: 555 1929 3785
Fragments arranged by size:
3785 1929 555
Acc65I G'GTAC_C
Cuts at: 861 2505 3285 861
Size: 1644 780 3845
Fragments arranged by size:
3845 1644 780
AclI AA'CG_TT
Cuts at: 6109 6109
Size: 6269
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1605 1852 2972 4094 4526 4879 5541 1111
Size: 494 247 1120 1122 432 353 662 1839
Fragments arranged by size:
1839 1122 1120 662 494 432 353 247
AfeI AGC'GCT
Cuts at: 258 258
Size: 6269
AflII C'TTAA_G
Cuts at: 3073 3073
Size: 6269
AflIII A'CryG_T
Cuts at: 1783 2085 3621 5421 5702 1783
Size: 302 1536 1800 281 2350
Fragments arranged by size:
2350 1800 1536 302 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 257 257
Size: 6269
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 499 531 2416 2448 499
Size: 32 1885 32 4320
Fragments arranged by size:
4320 1885 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1510 2031 3574 5012 1510
Size: 521 1543 1438 2767
Fragments arranged by size:
2767 1543 1438 521
ApaLI G'TGCA_C
Cuts at: 5107 5107
Size: 6269
ApoI r'AATT_y
Cuts at: 847 1003 1084 2471 2837 3273 5622 5878 847
Size: 156 81 1387 366 436 2349 256 1238
Fragments arranged by size:
2349 1387 1238 436 366 256 156 81
AseI AT'TA_AT
Cuts at: 20 1390 20
Size: 1370 4899
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6269
AvaI C'yCGr_G
Cuts at: 145 3289 145
Size: 3144 3125
AvrII C'CTAG_G
Cuts at: 732 732
Size: 6269
BamHI G'GATC_C
Cuts at: 3294 3294
Size: 6269
BanI G'GyrC_C
Cuts at: 476 861 2505 3285 3699 3947 3982 476
Size: 385 1644 780 414 248 35 2763
Fragments arranged by size:
2763 1644 780 414 385 248 35
BanII G_rGCy'C
Cuts at: 2789 3098 3283 4313 2789
Size: 309 185 1030 4745
Fragments arranged by size:
4745 1030 309 185
BbeI G_GCGC'C
Cuts at: 480 3703 3951 480
Size: 3223 248 2798
Fragments arranged by size:
3223 2798 248
BbsI GAAGACnn'nnnn_
Cuts at: 448 620 2164 2264 448
Size: 172 1544 100 4453
Fragments arranged by size:
4453 1544 172 100
BbvCI CC'TCA_GC
Cuts at: 1354 1354
Size: 6269
BciVI GTATCCnnnnn_n'
Cuts at: 721 2217 4167 5223 6269 721
Size: 1496 1950 1056 1046 721
Fragments arranged by size:
1950 1496 1056 1046 721
BclI T'GATC_A
Cuts at: 3789 3789
Size: 6269
BfrBI ATG'CAT
Cuts at: 1536 1738 2344 1536
Size: 202 606 5461
Fragments arranged by size:
5461 606 202
BglI GCCn_nnn'nGGC
Cuts at: 221 221
Size: 6269
BglII A'GATC_T
Cuts at: 874 1863 3784 5821 874
Size: 989 1921 2037 1322
Fragments arranged by size:
2037 1921 1322 989
BlpI GC'TnA_GC
Cuts at: 3381 3381
Size: 6269
Bme1580I G_kGCm'C
Cuts at: 3894 3987 5111 3894
Size: 93 1124 5052
Fragments arranged by size:
5052 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3481 3562 3897 6041 3481
Size: 81 335 2144 3709
Fragments arranged by size:
3709 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1337 4636 1337
Size: 3299 2970
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2773 3082 2720
Size: 53 309 5907
Fragments arranged by size:
5907 309 53
Bpu10I CC'TnA_GC
Cuts at: 1354 2519 1354
Size: 1165 5104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3445 4324 5038 5336 3445
Size: 879 714 298 4378
Fragments arranged by size:
4378 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5973 5973
Size: 6269
BsaAI yAC'GTr
Cuts at: 3622 3622
Size: 6269
BsaBI GATnn'nnATC
Cuts at: 6 1535 1850 2027 3783 5820 6
Size: 1529 315 177 1756 2037 455
Fragments arranged by size:
2037 1756 1529 455 315 177
BsaHI Gr'CG_yC
Cuts at: 224 272 477 3700 3948 4650 6146 224
Size: 48 205 3223 248 702 1496 347
Fragments arranged by size:
3223 1496 702 347 248 205 48
BsaWI w'CCGG_w
Cuts at: 3458 3979 4657 5068 5215 5489 3458
Size: 521 678 411 147 274 4238
Fragments arranged by size:
4238 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 568 2384 3038 3117 568
Size: 1816 654 79 3720
Fragments arranged by size:
3720 1816 654 79
BseYI C'CCAG_C
Cuts at: 3694 4452 5117 5525 3694
Size: 758 665 408 4438
Fragments arranged by size:
4438 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 122 179 3811 3857 5087 104
Size: 18 57 3632 46 1230 1286
Fragments arranged by size:
3632 1286 1230 57 46 18
BsiHKAI G_wGCw'C
Cuts at: 1887 2789 3098 3283 4061 4251 5111 1887
Size: 902 309 185 778 190 860 3045
Fragments arranged by size:
3045 902 860 778 309 190 185
BsmI GAATG_Cn'
Cuts at: 2284 2284
Size: 6269
BsmBI CGTCTCn'nnnn_
Cuts at: 917 917
Size: 6269
Bsp1286I G_dGCh'C
Cuts at: 1887 2789 3098 3283 3894 3987 4061 4251 4313
Size: 902 309 185 611 93 74 190 62
Cuts at: 4313 5111 1887
Size: 798 3045
Fragments arranged by size:
3045 902 798 611 309 190 185 93
74 62
BspHI T'CATG_A
Cuts at: 4701 4701
Size: 6269
BspMI ACCTGCnnnn'nnnn_
Cuts at: 438 1679 3319 3533 3835 4216 438
Size: 1241 1640 214 302 381 2491
Fragments arranged by size:
2491 1640 1241 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4561 4615 6099 6264 50
Size: 4511 54 1484 165 55
Fragments arranged by size:
4511 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3095 4181 3095
Size: 1086 5183
BsrFI r'CCGG_y
Cuts at: 4267 4448 4267
Size: 181 6088
BssHII G'CGCG_C
Cuts at: 152 152
Size: 6269
BssSI C'ACGA_G
Cuts at: 1607 4540 5248 1607
Size: 2933 708 2628
Fragments arranged by size:
2933 2628 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3646 3646
Size: 6269
BstBI TT'CG_AA
Cuts at: 2249 4630 2249
Size: 2381 3888
BstYI r'GATC_y
Cuts at: 1 336 874 1863 3294 3761 3784 4119 4365
Size: 335 538 989 1431 467 23 335 246
Cuts at: 4365 4671 4683 4769 5821 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1431 1052 989 538 467 449 335 335
306 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1379 1379
Size: 6269
BtgI C'CryG_G
Cuts at: 116 116
Size: 6269
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4219 4400 4563 4219
Size: 181 163 5925
Fragments arranged by size:
5925 181 163
BtsI GCAGTG_nn'
Cuts at: 3512 3512
Size: 6269
ClaI AT'CG_AT
Cuts at: 2129 2129
Size: 6269
DraI TTT'AAA
Cuts at: 3268 3268
Size: 6269
DraIII CAC_nnn'GTG
Cuts at: 2294 2294
Size: 6269
DrdI GACnn_nn'nnGTC
Cuts at: 3975 5319 3975
Size: 1344 4925
EaeI y'GGCC_r
Cuts at: 119 251 3854 4028 4419 4446 5791 119
Size: 132 3603 174 391 27 1345 597
Fragments arranged by size:
3603 1345 597 391 174 132 27
EagI C'GGCC_G
Cuts at: 119 3854 119
Size: 3735 2534
EarI CTCTTCn'nnn_
Cuts at: 557 749 764 1130 2366 2588 4292 4502 557
Size: 192 15 366 1236 222 1704 210 2324
Fragments arranged by size:
2324 1704 1236 366 222 210 192 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 119 4477 5219 5365 6079 119
Size: 4358 742 146 714 309
Fragments arranged by size:
4358 742 714 309 146
EcoICRI GAG'CTC
Cuts at: 2787 3096 3281 2787
Size: 309 185 5775
Fragments arranged by size:
5775 309 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1605 1852 2720 2773 2972 3082 4094 4526
Size: 494 247 868 53 199 110 1012 432
Cuts at: 4526 4879 5541 1111
Size: 353 662 1839
Fragments arranged by size:
1839 1012 868 662 494 432 353 247
199 110 53
EcoO109I rG'GnC_Cy
Cuts at: 3408 3408
Size: 6269
EcoRI G'AATT_C
Cuts at: 847 1084 2837 3273 847
Size: 237 1753 436 3843
Fragments arranged by size:
3843 1753 436 237
EcoRV GAT'ATC
Cuts at: 2127 2127
Size: 6269
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 525 929 1882 2032 2575 2632 4288 525
Size: 404 953 150 543 57 1656 2506
Fragments arranged by size:
2506 1656 953 543 404 150 57
FspI TGC'GCA
Cuts at: 4050 4050
Size: 6269
HaeII r_GCGC'y
Cuts at: 260 480 3703 3951 5181 5952 260
Size: 220 3223 248 1230 771 577
Fragments arranged by size:
3223 1230 771 577 248 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 607 639 671 1135 1167 1601 1633
Size: 32 289 32 32 464 32 434 32
Cuts at: 1633 2215 2247 2796 2828 4111 4141 4143 4173
Size: 582 32 549 32 1283 30 2 30
Cuts at: 4173 286
Size: 2382
Fragments arranged by size:
2382 1283 582 549 464 434 289 32
32 32 32 32 32 32 30 30
2
HincII GTy'rAC
Cuts at: 3308 3308
Size: 6269
HindIII A'AGCT_T
Cuts at: 2614 2614
Size: 6269
KasI G'GCGC_C
Cuts at: 476 3699 3947 476
Size: 3223 248 2798
Fragments arranged by size:
3223 2798 248
KpnI G_GTAC'C
Cuts at: 865 2509 3289 865
Size: 1644 780 3845
Fragments arranged by size:
3845 1644 780
MfeI C'AATT_G
Cuts at: 2637 2637
Size: 6269
MluI A'CGCG_T
Cuts at: 1783 5702 1783
Size: 3919 2350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 472 1673 5028 5212 472
Size: 1201 3355 184 1529
Fragments arranged by size:
3355 1529 1201 184
MscI TGG'CCA
Cuts at: 4030 4030
Size: 6269
MslI CAynn'nnrTG
Cuts at: 1456 4258 4385 1456
Size: 2802 127 3340
Fragments arranged by size:
3340 2802 127
MspA1I CmG'CkG
Cuts at: 118 185 2761 2910 3380 3694 4054 4838 5083
Size: 67 2576 149 470 314 360 784 245
Cuts at: 5083 118
Size: 1304
Fragments arranged by size:
2576 1304 784 470 360 314 245 149
67
NaeI GCC'GGC
Cuts at: 4450 4450
Size: 6269
NarI GG'CG_CC
Cuts at: 477 3700 3948 477
Size: 3223 248 2798
Fragments arranged by size:
3223 2798 248
NdeI CA'TA_TG
Cuts at: 803 1262 2922 803
Size: 459 1660 4150
Fragments arranged by size:
4150 1660 459
NgoMIV G'CCGG_C
Cuts at: 4448 4448
Size: 6269
NsiI A_TGCA'T
Cuts at: 1538 1740 2346 1538
Size: 202 606 5461
Fragments arranged by size:
5461 606 202
NspI r_CATG'y
Cuts at: 1668 2089 3322 3822 5425 1668
Size: 421 1233 500 1603 2512
Fragments arranged by size:
2512 1603 1233 500 421
PciI A'CATG_T
Cuts at: 2085 5421 2085
Size: 3336 2933
PfoI T'CCnGG_A
Cuts at: 2789 2789
Size: 6269
PmeI GTTT'AAAC
Cuts at: 3268 3268
Size: 6269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 4715
Size: 32 6237
PsiI TTA'TAA
Cuts at: 1930 2243 1930
Size: 313 5956
PstI C_TGCA'G
Cuts at: 1690 2166 3316 4001 1690
Size: 476 1150 685 3958
Fragments arranged by size:
3958 1150 685 476
PvuI CG_AT'CG
Cuts at: 104 179 104
Size: 75 6194
PvuII CAG'CTG
Cuts at: 2761 2910 3694 4054 2761
Size: 149 784 360 4976
Fragments arranged by size:
4976 784 360 149
RsrII CG'GwC_CG
Cuts at: 4464 4464
Size: 6269
SacI G_AGCT'C
Cuts at: 2789 3098 3283 2789
Size: 309 185 5775
Fragments arranged by size:
5775 309 185
SacII CC_GC'GG
Cuts at: 119 119
Size: 6269
SalI G'TCGA_C
Cuts at: 3306 3306
Size: 6269
SapI GCTCTTCn'nnn_
Cuts at: 557 2366 4292 4502 557
Size: 1809 1926 210 2324
Fragments arranged by size:
2324 1926 1809 210
SbfI CC_TGCA'GG
Cuts at: 3316 3316
Size: 6269
ScaI AGT'ACT
Cuts at: 1613 1684 5786 1613
Size: 71 4102 2096
Fragments arranged by size:
4102 2096 71
SexAI A'CCwGG_T
Cuts at: 1509 1509
Size: 6269
SfcI C'TryA_G
Cuts at: 33 1686 1943 2162 2262 3312 3997 4965 5156
Size: 1653 257 219 100 1050 685 968 191
Cuts at: 5156 33
Size: 1146
Fragments arranged by size:
1653 1146 1050 968 685 257 219 191
100
SfoI GGC'GCC
Cuts at: 478 3701 3949 478
Size: 3223 248 2798
Fragments arranged by size:
3223 2798 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6269
SmaI CCC'GGG
Cuts at: 3291 3291
Size: 6269
SmlI C'TyrA_G
Cuts at: 3073 3424 4339 5053 5315 3073
Size: 351 915 714 262 4027
Fragments arranged by size:
4027 915 714 351 262
SpeI A'CTAG_T
Cuts at: 67 1245 3261 67
Size: 1178 2016 3075
Fragments arranged by size:
3075 2016 1178
SphI G_CATG'C
Cuts at: 3322 3822 3322
Size: 500 5769
SspI AAT'ATT
Cuts at: 290 2755 290
Size: 2465 3804
StyI C'CwwG_G
Cuts at: 732 1230 2387 3403 3757 732
Size: 498 1157 1016 354 3244
Fragments arranged by size:
3244 1157 1016 498 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1895 2400 3852 3985 4444 4653 1895
Size: 505 1452 133 459 209 3511
Fragments arranged by size:
3511 1452 505 459 209 133
TatI w'GTAC_w
Cuts at: 1611 1682 5784 1611
Size: 71 4102 2096
Fragments arranged by size:
4102 2096 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5786 5818 5786
Size: 32 6237
Tth111I GACn'n_nGTC
Cuts at: 4066 4066
Size: 6269
XbaI T'CTAG_A
Cuts at: 3300 3300
Size: 6269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3087 3764 3087
Size: 677 5592
XmaI C'CCGG_G
Cuts at: 3289 3289
Size: 6269
XmnI GAAnn'nnTTC
Cuts at: 2057 2360 2057
Size: 303 5966
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PciI PfoI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AleI ApaI AscI BaeI
BcgI BmgBI BmtI BsaXI BsgI BsiWI BspEI BsrGI
BstEII BstXI Bsu36I CspCI EcoNI FseI FspAI HpaI
NcoI NheI NotI NruI PacI PasI PflMI PmlI
PpuMI PshAI PspOMI PspXI PsrI SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI